Strain Name:
C57BL/6J-MtgxR2235Btlr/Mmmh
Stock Number:
040236-MU
Citation ID:
RRID:MMRRC_040236-MU
Other Names:
R2235 (G1), C57BL/6J-MtgxR2235Btlr
Major Collection:

Gene Information

Chrm4
Name: cholinergic receptor, muscarinic 4
Synonyms: muscarinic acetylcholine receptor 4, Chrm-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12672
HGNC: HGNC:1953
Homologene: 20192
Ubp1
Name: upstream binding protein 1
Synonyms: LBP-1a, NF2d9, LBP-1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 22221
VEGA: 9
Homologene: 8435
Igf1r
Name: insulin-like growth factor I receptor
Synonyms: A330103N21Rik, CD221, line 186, IGF-1R, hyft
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 16001
HGNC: HGNC:5465
Homologene: 30997
Smg7
Name: Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans)
Synonyms: 9430023P16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226517
Homologene: 32235
Pds5a
Name: PDS5 cohesin associated factor A
Synonyms: 9030416H16Rik, E230024D05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 71521
Homologene: 22877
Racgap1
Name: Rac GTPase-activating protein 1
Synonyms: Band25, GTPase, gtl11, MgcRacGAP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 26934
HGNC: HGNC:9804
Homologene: 8077
Tpr
Name: translocated promoter region, nuclear basket protein
Synonyms: 2610029M07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 108989
Homologene: 37753
Rnf216
Name: ring finger protein 216
Synonyms: Ubce7ip1, UIP83, F830018F18Rik, 2810055G22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 108086
Homologene: 19442
Crh
Name: corticotropin releasing hormone
Synonyms: corticotropin releasing factor, LOC383938, corticotropin-releasing factor, CRF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 12918
HGNC: HGNC:2355
Homologene: 599
Ralgapa1
Name: Ral GTPase activating protein, alpha subunit 1
Synonyms: Tulip1, 2310003F20Rik, 4930400K19Rik, Garnl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 56784
VEGA: 12
Homologene: 84805
Acbd6
Name: acyl-Coenzyme A binding domain containing 6
Synonyms: 0610010G04Rik, 2610100E10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 72482
Homologene: 12465
Sox4
Name: SRY (sex determining region Y)-box 4
Synonyms: Sox-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20677
Homologene: 2338
Pld3
Name: phospholipase D family, member 3
Synonyms: Sam-9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18807
Homologene: 7893
Smap1
Name: small ArfGAP 1
Synonyms: 4921514B13Rik, 1700056O10Rik, 4921525H11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98366
Homologene: 134676
E2f4
Name: E2F transcription factor 4
Synonyms: 2010111M04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 104394
HGNC: HGNC:3118
Homologene: 1471
Dync1i2
Name: dynein cytoplasmic 1 intermediate chain 2
Synonyms: 3110079H08Rik, Dncic2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 13427
HGNC: HGNC:2964
Homologene: 37921
Bach1
Name: BTB and CNC homology 1, basic leucine zipper transcription factor 1
Synonyms: 6230421P05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 12013
HGNC: HGNC:935
Homologene: 916
Tenm3
Name: teneurin transmembrane protein 3
Synonyms: Ten-m3, 2610100B16Rik, Odz3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 23965
Homologene: 22673
Atad2b
Name: ATPase family, AAA domain containing 2B
Synonyms: D530031C13Rik, 1110014E10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 320817
VEGA: 12
Homologene: 86351
Vit
Name: vitrin
Synonyms: AKH, 1700110E08Rik, 1700052E02Rik, 2810429K11Rik, akhirin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 74199
VEGA: 17
Homologene: 24942
Lmo2
Name: LIM domain only 2
Synonyms: Rbtn2, Rhom-2, Rbtn-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16909
HGNC: HGNC:6642
Homologene: 4072
Nfkbid
Name: nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, delta
Synonyms: IkappaBNS
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 243910
Homologene: 13101
Col1a2
Name: collagen, type I, alpha 2
Synonyms: Col1a-2, Cola-2, Cola2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12843
HGNC: HGNC:2198
Homologene: 69
Fam160a1
Name: family with sequence similarity 160, member A1
Synonyms: 9930021J17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229488
Homologene: 85149
Dnah6
Name: dynein, axonemal, heavy chain 6
Synonyms: A730004I20Rik, Dnahc6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 330355
HGNC: HGNC:2951
Homologene: 15221
Zfp990
Name: zinc finger protein 990
Synonyms: Gm13225
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 101056073
Sis
Name: sucrase isomaltase (alpha-glucosidase)
Synonyms: sucrase-isomaltase, 2010204N08Rik, Si-s
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 69983
Homologene: 37424
Kcnab1
Name: potassium voltage-gated channel, shaker-related subfamily, beta member 1
Synonyms: Akr8a8, Kvbeta1.1, mKv(beta)1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 16497
HGNC: HGNC:6228
Homologene: 56491
Usp40
Name: ubiquitin specific peptidase 40
Synonyms: B230215L03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227334
Homologene: 32400
Spaca6
Name: sperm acrosome associated 6
Synonyms: Ncrna00085, B230206P06Rik, 4930546H06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 75202
Homologene: 53443
Prph2
Name: peripherin 2
Synonyms: Nmf193, rds, Tspan22, Rd2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 19133
HGNC: HGNC:9942
Homologene: 273
Slc4a11
Name: solute carrier family 4, sodium bicarbonate transporter-like, member 11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 269356
Homologene: 12931
A2m
Name: alpha-2-macroglobulin
Synonyms: A2mp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232345
HGNC: HGNC:7
Homologene: 37248
Adcy2
Name: adenylate cyclase 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 210044
VEGA: 13
HGNC: HGNC:233
Homologene: 75133
Olfr1346
Name: olfactory receptor 1346
Synonyms: GA_x6K02T2QGBW-3203957-3204898, MOR103-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258918
Homologene: 74134
Osbpl6
Name: oxysterol binding protein-like 6
Synonyms: ORP-6, 1110062M20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99031
Homologene: 101447
Cts6
Name: cathepsin 6
Synonyms: 1600022N02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 58518
Homologene: 75165
Mto1
Name: mitochondrial tRNA translation optimization 1
Synonyms: 5730419A02Rik, 2310039H01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 68291
Homologene: 5876
Zkscan6
Name: zinc finger with KRAB and SCAN domains 6
Synonyms: D11Ertd714e, Zfp535, KOX11, 1700128E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 52712
Homologene: 12119
Trim65
Name: tripartite motif-containing 65
Synonyms: 4732463G12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 338364
Homologene: 27844
Hid1
Name: HID1 domain containing
Synonyms: C630004H02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217310
Homologene: 12737
Tbx18
Name: T-box18
Synonyms: 2810012F10Rik, 2810404D13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 76365
Homologene: 11384
Lrrc49
Name: leucine rich repeat containing 49
Synonyms: D430025H09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 102747
Homologene: 9782
Tmprss11c
Name: transmembrane protease, serine 11c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 435845
Homologene: 78847
Myot
Name: myotilin
Synonyms: Ttid, 5530402I04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 58916
Homologene: 4942
Itprid1
Name: ITPR interacting domain containing 1
Synonyms: D530004J12Rik, Ccdc129
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232016
Homologene: 52344
Iglon5
Name: IgLON family member 5
Synonyms: A230106M20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 210094
Homologene: 46295
Kcnt2
Name: potassium channel, subfamily T, member 2
Synonyms: E330038N15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240776
Homologene: 16121
Scgb1b27
Name: secretoglobin, family 1B, member 27
Synonyms: Sal-1, Abp, Abpa27, Tcp, Abpa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 11354
Homologene: 114479
Vmn2r124
Name: vomeronasal 2, receptor 124
Synonyms: Gm7196, Vmn2r-ps113
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 637021
Homologene: 115024
Sel1l2
Name: sel-1 suppressor of lin-12-like 2 (C. elegans)
Synonyms: LOC228684
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228684
Homologene: 16802
Hyou1
Name: hypoxia up-regulated 1
Synonyms: Grp170, Orp150, CBP-140, Cab140, 140 kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12282
Homologene: 4658
Alpk1
Name: alpha-kinase 1
Synonyms: 8430410J10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 71481
Homologene: 11849
Clca1
Name: chloride channel accessory 1
Synonyms: gob-5, gob5, Clca3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 23844
HGNC: HGNC:2015
Homologene: 984
Traf5
Name: TNF receptor-associated factor 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 22033
Homologene: 27079
Olfr518
Name: olfactory receptor 518
Synonyms: GA_x6K02T2PBJ9-11211854-11210853, MOR268-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258303
HGNC: HGNC:8162
Homologene: 133601
Ly6h
Name: lymphocyte antigen 6 complex, locus H
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 23934
HGNC: HGNC:6728
Homologene: 1758
Thap4
Name: THAP domain containing 4
Synonyms: 2010320B01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 67026
Homologene: 12075
Fndc11
Name: fibronectin type III domain containing 11
Synonyms: 1700026M20Rik, BC051628
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 332713
Homologene: 11442
BC035947
Name: cDNA sequence BC035947
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Csta1
Name: cystatin A1
Synonyms: Csta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 209294
HGNC: HGNC:2481
Homologene: 3819
Otc
Name: ornithine transcarbamylase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 18416
HGNC: HGNC:8512
Homologene: 446
Cldn34b3
Name: claudin 34B3
Synonyms: Gm4937
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 238829
Homologene: 79770
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 23,859,058 bp
  • A to T, chromosome 1 at 78,497,962 bp
  • A to G, chromosome 1 at 88,004,325 bp
  • G to T, chromosome 1 at 93,725,212 bp
  • T to C, chromosome 1 at 140,573,693 bp
  • T to A, chromosome 1 at 150,442,092 bp
  • A to T, chromosome 1 at 152,868,313 bp
  • A to G, chromosome 1 at 155,558,708 bp
  • T to C, chromosome 1 at 192,054,391 bp
  • C to T, chromosome 2 at 71,249,420 bp
  • T to C, chromosome 2 at 76,586,769 bp
  • A to G, chromosome 2 at 91,928,530 bp
  • T to C, chromosome 2 at 103,981,062 bp
  • T to C, chromosome 2 at 130,685,624 bp
  • T to C, chromosome 2 at 140,244,165 bp
  • A to G, chromosome 2 at 181,222,274 bp
  • A to T, chromosome 3 at 19,693,932 bp
  • T to A, chromosome 3 at 65,319,467 bp
  • A to T, chromosome 3 at 72,913,194 bp
  • A to T, chromosome 3 at 85,661,101 bp
  • T to C, chromosome 3 at 127,680,920 bp
  • G to T, chromosome 3 at 145,009,068 bp
  • T to A, chromosome 4 at 145,537,891 bp
  • A to T, chromosome 5 at 65,654,098 bp
  • G to T, chromosome 5 at 86,282,086 bp
  • G to A, chromosome 5 at 143,090,926 bp
  • G to A, chromosome 6 at 4,518,822 bp
  • A to T, chromosome 6 at 55,897,812 bp
  • T to C, chromosome 6 at 73,100,085 bp
  • T to C, chromosome 6 at 121,642,064 bp
  • T to C, chromosome 7 at 6,474,442 bp
  • G to A, chromosome 7 at 27,541,107 bp
  • T to A, chromosome 7 at 30,425,859 bp
  • T to C, chromosome 7 at 34,021,824 bp
  • T to C, chromosome 7 at 43,480,638 bp
  • T to A, chromosome 7 at 68,212,080 bp
  • A to G, chromosome 7 at 108,880,965 bp
  • T to C, chromosome 8 at 48,276,169 bp
  • T to A, chromosome 8 at 105,298,651 bp
  • C to T, chromosome 9 at 44,389,091 bp
  • A to T, chromosome 9 at 60,598,157 bp
  • A to C, chromosome 9 at 78,457,564 bp
  • G to T, chromosome 9 at 87,724,350 bp
  • T to G, chromosome 9 at 113,964,644 bp
  • T to C, chromosome 11 at 65,828,272 bp
  • A to G, chromosome 11 at 115,351,119 bp
  • G to T, chromosome 11 at 116,130,677 bp
  • T to G, chromosome 12 at 5,006,745 bp
  • A to T, chromosome 12 at 55,717,071 bp
  • C to T, chromosome 13 at 28,952,630 bp
  • T to A, chromosome 13 at 61,195,433 bp
  • A to T, chromosome 13 at 68,668,492 bp
  • A to G, chromosome 15 at 75,565,189 bp
  • A to G, chromosome 15 at 99,626,536 bp
  • C to T, chromosome 16 at 36,125,075 bp
  • A to G, chromosome 16 at 87,720,113 bp
  • T to C, chromosome 17 at 17,838,245 bp
  • A to T, chromosome 17 at 18,049,665 bp
  • A to T, chromosome 17 at 46,911,166 bp
  • T to C, chromosome 17 at 78,605,438 bp
  • A to G, chromosome 18 at 44,354,272 bp
  • A to G, chromosome X at 10,303,367 bp
  • A to T, chromosome X at 76,267,224 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2235 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040236-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.