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Strain Name:
C57BL/6J-MtgxR2235Btlr/Mmmh
Stock Number:
040236-MU
Citation ID:
RRID:MMRRC_040236-MU
Other Names:
R2235 (G1), C57BL/6J-MtgxR2235Btlr
Major Collection:

Strain Information

Chrm4
Name: cholinergic receptor, muscarinic 4
Synonyms: Chrm-4, muscarinic acetylcholine receptor 4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 12672
HGNC: HGNC:1953
Homologene: 20192
Ubp1
Name: upstream binding protein 1
Synonyms: NF2d9, LBP-1b, LBP-1a
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 22221
VEGA: 9
Homologene: 8435
Igf1r
Name: insulin-like growth factor I receptor
Synonyms: CD221, IGF-1R, line 186, hyft, A330103N21Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 16001
HGNC: HGNC:5465
Homologene: 30997
Smg7
Name: SMG7 nonsense mediated mRNA decay factor
Synonyms: 9430023P16Rik, Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans)
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226517
Homologene: 32235
Pds5a
Name: PDS5 cohesin associated factor A
Synonyms: E230024D05Rik, 9030416H16Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 71521
Homologene: 22877
Racgap1
Name: Rac GTPase-activating protein 1
Synonyms: MgcRacGAP, GTPase, Band25, gtl11
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 26934
HGNC: HGNC:9804
Homologene: 8077
Tpr
Name: translocated promoter region, nuclear basket protein
Synonyms: 2610029M07Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 108989
Homologene: 37753
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 23,859,058 bp
  • A to T, chromosome 1 at 78,497,962 bp
  • A to G, chromosome 1 at 88,004,325 bp
  • G to T, chromosome 1 at 93,725,212 bp
  • T to C, chromosome 1 at 140,573,693 bp
  • T to A, chromosome 1 at 150,442,092 bp
  • A to T, chromosome 1 at 152,868,313 bp
  • A to G, chromosome 1 at 155,558,708 bp
  • T to C, chromosome 1 at 192,054,391 bp
  • C to T, chromosome 2 at 71,249,420 bp
  • T to C, chromosome 2 at 76,586,769 bp
  • A to G, chromosome 2 at 91,928,530 bp
  • T to C, chromosome 2 at 103,981,062 bp
  • T to C, chromosome 2 at 130,685,624 bp
  • T to C, chromosome 2 at 140,244,165 bp
  • A to G, chromosome 2 at 181,222,274 bp
  • A to T, chromosome 3 at 19,693,932 bp
  • T to A, chromosome 3 at 65,319,467 bp
  • A to T, chromosome 3 at 72,913,194 bp
  • A to T, chromosome 3 at 85,661,101 bp
  • T to C, chromosome 3 at 127,680,920 bp
  • G to T, chromosome 3 at 145,009,068 bp
  • T to A, chromosome 4 at 145,537,891 bp
  • A to T, chromosome 5 at 65,654,098 bp
  • G to T, chromosome 5 at 86,282,086 bp
  • G to A, chromosome 5 at 143,090,926 bp
  • G to A, chromosome 6 at 4,518,822 bp
  • A to T, chromosome 6 at 55,897,812 bp
  • T to C, chromosome 6 at 73,100,085 bp
  • T to C, chromosome 6 at 121,642,064 bp
  • T to C, chromosome 7 at 6,474,442 bp
  • G to A, chromosome 7 at 27,541,107 bp
  • T to A, chromosome 7 at 30,425,859 bp
  • T to C, chromosome 7 at 34,021,824 bp
  • T to C, chromosome 7 at 43,480,638 bp
  • T to A, chromosome 7 at 68,212,080 bp
  • A to G, chromosome 7 at 108,880,965 bp
  • T to C, chromosome 8 at 48,276,169 bp
  • T to A, chromosome 8 at 105,298,651 bp
  • C to T, chromosome 9 at 44,389,091 bp
  • A to T, chromosome 9 at 60,598,157 bp
  • A to C, chromosome 9 at 78,457,564 bp
  • G to T, chromosome 9 at 87,724,350 bp
  • T to G, chromosome 9 at 113,964,644 bp
  • T to C, chromosome 11 at 65,828,272 bp
  • A to G, chromosome 11 at 115,351,119 bp
  • G to T, chromosome 11 at 116,130,677 bp
  • T to G, chromosome 12 at 5,006,745 bp
  • A to T, chromosome 12 at 55,717,071 bp
  • C to T, chromosome 13 at 28,952,630 bp
  • T to A, chromosome 13 at 61,195,433 bp
  • A to T, chromosome 13 at 68,668,492 bp
  • A to G, chromosome 15 at 75,565,189 bp
  • A to G, chromosome 15 at 99,626,536 bp
  • C to T, chromosome 16 at 36,125,075 bp
  • A to G, chromosome 16 at 87,720,113 bp
  • T to C, chromosome 17 at 17,838,245 bp
  • A to T, chromosome 17 at 18,049,665 bp
  • A to T, chromosome 17 at 46,911,166 bp
  • T to C, chromosome 17 at 78,605,438 bp
  • A to G, chromosome 18 at 44,354,272 bp
  • A to G, chromosome X at 10,303,367 bp
  • A to T, chromosome X at 76,267,224 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2235 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040236-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.