Strain Name:
C57BL/6J-MtgxR2283Btlr/Mmmh
Stock Number:
040282-MU
Citation ID:
RRID:MMRRC_040282-MU
Other Names:
R2283 (G1), C57BL/6J-MtgxR2283Btlr
Major Collection:

Strain Information

Asxl2
Name: ASXL transcriptional regulator 2
Synonyms: 4930556B16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 75302
Homologene: 10102
Anapc1
Name: anaphase promoting complex subunit 1
Synonyms: tsg24, Apc1, 2610021O03Rik, Mcpr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17222
Homologene: 7414
Nav2
Name: neuron navigator 2
Synonyms: POMFIL2, Unc53H2, HELAD1, RAINB2, 5330421F07Rik, Rainb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 78286
Homologene: 52330
Rsf1
Name: remodeling and spacing factor 1
Synonyms: p325, XAP8, Hbxap, C030033M12Rik, 4832420A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233532
Homologene: 41142
Elp1
Name: elongator complex protein 1
Synonyms: 3110040G09Rik, C78473, IKAP, Ikbkap
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230233
HGNC: HGNC:5959
Homologene: 2699
Fas
Name: Fas (TNF receptor superfamily member 6)
Synonyms: CD95, APO-1, TNFR6, Tnfrsf6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 14102
VEGA: 19
Homologene: 27
Kif26a
Name: kinesin family member 26A
Synonyms: N-11 kinesin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 668303
Homologene: 18970
Sp3
Name: trans-acting transcription factor 3
Synonyms: D130027J01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20687
Homologene: 7952
Zfp931
Name: zinc finger protein 931
Synonyms: 2810021G02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 353208
Homologene: 136272
Clec18a
Name: C-type lectin domain family 18, member A
Synonyms: Mrcl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 353287
Homologene: 18755
Ppa1
Name: pyrophosphatase (inorganic) 1
Synonyms: 2010317E03Rik, Pyp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 67895
HGNC: HGNC:9226
Homologene: 5356
Bhmt
Name: betaine-homocysteine methyltransferase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 12116
HGNC: HGNC:1047
Homologene: 1295
Zfp608
Name: zinc finger protein 608
Synonyms: 4932417D18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 269023
VEGA: 18
Homologene: 18485
Fras1
Name: Fraser extracellular matrix complex subunit 1
Synonyms: bl, E130113P14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231470
Homologene: 23516
Adgb
Name: androglobin
Synonyms: 9130014G24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 215772
Homologene: 100289
St8sia3
Name: ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 3
Synonyms: ST8SiaIII, Siat8c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 20451
Homologene: 8285
Nuggc
Name: nuclear GTPase, germinal center associated
Synonyms: LOC239151, SLIP-GC, Gm600
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 100503545
Homologene: 72641
Tex10
Name: testis expressed gene 10
Synonyms: clone 18330, 2810462N03Rik, 2610206N19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 269536
Homologene: 32361
Dlg5
Name: discs large MAGUK scaffold protein 5
Synonyms: 4933429D20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 71228
HGNC: HGNC:2904
Homologene: 3486
Krt2
Name: keratin 2
Synonyms: Krt2-2, Krt2e, Krt2-17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 16681
HGNC: HGNC:6439
Cel
Name: carboxyl ester lipase
Synonyms: BAL, 1810036E18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12613
HGNC: HGNC:1848
Homologene: 37529
Mmp8
Name: matrix metallopeptidase 8
Synonyms: Collagenase-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 17394
VEGA: 9
HGNC: HGNC:7175
Homologene: 22482
Hrh1
Name: histamine receptor H1
Synonyms: Bphs, Hir
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 15465
HGNC: HGNC:5182
Homologene: 668
Aadacl2
Name: arylacetamide deacetylase like 2
Synonyms: EG639634
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 639634
Homologene: 28634
Ift88
Name: intraflagellar transport 88
Synonyms: Tg737, polaris, Oak Ridge polycystic kidneys, orpk, IFT88, fxo, TgN737Rpw, Tg737Rpw, Ttc10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 21821
Homologene: 4761
Masp2
Name: mannan-binding lectin serine peptidase 2
Synonyms: MAp19, MASP-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 17175
HGNC: HGNC:6902
Homologene: 4819
Or2b6
Name: olfactory receptor family 2 subfamily B member 6
Synonyms: MOR256-11, GA_x6K02T2QHY8-11597382-11598323, Olfr11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218066
HGNC: HGNC:8241
Homologene: 40838
Pcsk4
Name: proprotein convertase subtilisin/kexin type 4
Synonyms: PC4, SPC5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 18551
HGNC: HGNC:8746
Homologene: 22495
Gpt2
Name: glutamic pyruvate transaminase (alanine aminotransferase) 2
Synonyms: ALT2, 4631422C05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 108682
Homologene: 68832
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 2 at 28,560,468 bp
  • A to C, chromosome 2 at 72,971,177 bp
  • A to T, chromosome 2 at 128,642,548 bp
  • A to G, chromosome 2 at 178,069,921 bp
  • T to C, chromosome 3 at 60,007,130 bp
  • A to T, chromosome 4 at 48,462,764 bp
  • T to C, chromosome 4 at 56,775,014 bp
  • A to G, chromosome 4 at 148,606,068 bp
  • T to C, chromosome 5 at 96,654,305 bp
  • C to T, chromosome 6 at 114,480,439 bp
  • G to T, chromosome 7 at 49,491,404 bp
  • G to GACGGCGGCT, chromosome 7 at 97,579,909 bp
  • A to G, chromosome 7 at 104,840,652 bp
  • T to A, chromosome 8 at 85,516,189 bp
  • A to G, chromosome 8 at 111,075,508 bp
  • A to G, chromosome 9 at 7,563,236 bp
  • T to C, chromosome 10 at 10,377,891 bp
  • G to A, chromosome 10 at 61,661,009 bp
  • T to C, chromosome 10 at 80,322,750 bp
  • A to G, chromosome 12 at 3,451,650 bp
  • T to C, chromosome 12 at 112,177,353 bp
  • G to A, chromosome 13 at 21,639,020 bp
  • G to A, chromosome 13 at 93,620,301 bp
  • G to A, chromosome 14 at 24,158,663 bp
  • T to C, chromosome 14 at 57,480,011 bp
  • T to C, chromosome 14 at 65,638,612 bp
  • T to C, chromosome 15 at 101,814,387 bp
  • T to A, chromosome 18 at 54,988,374 bp
  • G to T, chromosome 18 at 64,271,730 bp
  • G to A, chromosome 19 at 34,307,249 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2283 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040282-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.