Strain Name:
C57BL/6J-MtgxR2302Btlr/Mmmh
Stock Number:
040301-MU
Citation ID:
RRID:MMRRC_040301-MU
Other Names:
R2302 (G1), C57BL/6J-MtgxR2302Btlr
Major Collection:

Gene Information

Cdh3
Name: cadherin 3
Synonyms: Cadp, Pcad, P-cadherin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 12560
HGNC: HGNC:1762
Homologene: 20425
Pomt1
Name: protein-O-mannosyltransferase 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 99011
HGNC: HGNC:9202
Homologene: 68548
Zfp609
Name: zinc finger protein 609
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 214812
Homologene: 72220
Zzef1
Name: zinc finger, ZZ-type with EF hand domain 1
Synonyms: 8430405D05Rik, C130099L13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 195018
Homologene: 9027
Poldip3
Name: polymerase (DNA-directed), delta interacting protein 3
Synonyms: 1110008P04Rik, PDIP46
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 73826
Homologene: 13007
Hyls1
Name: HYLS1, centriolar and ciliogenesis associated
Synonyms: 3010015K02Rik, hydrolethalus syndrome 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 76832
Homologene: 82283
Prokr2
Name: prokineticin receptor 2
Synonyms: EG-VEGRF2, PKR2, Gpcr73l1, Gpr73l1, B830005M06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 246313
Homologene: 16368
Rab3b
Name: RAB3B, member RAS oncogene family
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 69908
HGNC: HGNC:9778
Homologene: 2149
Reck
Name: reversion-inducing-cysteine-rich protein with kazal motifs
Synonyms: St15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 53614
Homologene: 9622
Ank1
Name: ankyrin 1, erythroid
Synonyms: pale, Ank-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 11733
HGNC: HGNC:492
Homologene: 55427
Scn1a
Name: sodium channel, voltage-gated, type I, alpha
Synonyms: Nav1.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 20265
Homologene: 21375
Ttn
Name: titin
Synonyms: shru, connectin, mdm, 2310057K23Rik, D330041I19Rik, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, L56, 2310074I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22138
Homologene: 130650
Ahrr
Name: aryl-hydrocarbon receptor repressor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 11624
HGNC: HGNC:346
Homologene: 49215
Gsap
Name: gamma-secretase activating protein
Synonyms: A530088I07Rik, Pion
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 212167
Homologene: 45504
Myh8
Name: myosin, heavy polypeptide 8, skeletal muscle, perinatal
Synonyms: Myhs-p, 4832426G23Rik, Myhsp, MyHC-pn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 17885
HGNC: HGNC:7578
Homologene: 68256
Lrrc7
Name: leucine rich repeat containing 7
Synonyms: densin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 242274
Homologene: 10817
Ifngr1
Name: interferon gamma receptor 1
Synonyms: IFN-gamma R, IFN-gammaR, Ifngr, Ifgr, Nktar, CD119
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 15979
HGNC: HGNC:5439
Homologene: 359
Zscan20
Name: zinc finger and SCAN domains 20
Synonyms: Zfp31
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 269585
Homologene: 51463
Csmd3
Name: CUB and Sushi multiple domains 3
Synonyms: 4930500N14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 239420
Homologene: 65982
Kdm1b
Name: lysine (K)-specific demethylase 1B
Synonyms: Aof1, 4632428N09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 218214
Homologene: 14695
Slfn10-ps
Name: schlafen 10, pseudogene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 237887
Lhfpl2
Name: lipoma HMGIC fusion partner-like 2
Synonyms: vgim
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 218454
HGNC: HGNC:6588
Homologene: 4222
Svopl
Name: SV2 related protein homolog (rat)-like
Synonyms: 9430071P14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 320590
Homologene: 52168
Dhcr7
Name: 7-dehydrocholesterol reductase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 13360
HGNC: HGNC:2860
Homologene: 1042
Gbp9
Name: guanylate-binding protein 9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 236573
Homologene: 128731
Krt1
Name: keratin 1
Synonyms: Krt-2.1, Krt2-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 16678
VEGA: 15
HGNC: HGNC:6412
Homologene: 38146
Tbx5
Name: T-box 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 21388
Homologene: 160
Cutal
Name: cutA divalent cation tolerance homolog-like
Synonyms: D730039F16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 77996
Homologene: 66286
Mlc1
Name: megalencephalic leukoencephalopathy with subcortical cysts 1 homolog (human)
Synonyms: Kiaa0027-hp, WKL1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 170790
Homologene: 15775
Ppil6
Name: peptidylprolyl isomerase (cyclophilin)-like 6
Synonyms: 2900084F20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 73075
VEGA: 10
Homologene: 52155
Slco5a1
Name: solute carrier organic anion transporter family, member 5A1
Synonyms: A630033C23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 240726
Homologene: 57135
Cnn2
Name: calponin 2
Synonyms: h2-calponin, Calpo2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 12798
HGNC: HGNC:2156
Homologene: 3215
Mup4
Name: major urinary protein 4
Synonyms: Mup-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 17843
Homologene: 74304
Sftpd
Name: surfactant associated protein D
Synonyms: Sftp4, SP-D, Sfpd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 20390
VEGA: 14
Homologene: 2272
Agpat2
Name: 1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta)
Synonyms: BSCL1, LPAAT-beta, 2510002J07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 67512
HGNC: HGNC:325
Homologene: 4678
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 12,879,262 bp
  • A to T, chromosome 2 at 26,604,195 bp
  • G to A, chromosome 2 at 32,243,659 bp
  • A to T, chromosome 2 at 34,882,447 bp
  • T to C, chromosome 2 at 66,277,745 bp
  • A to T, chromosome 2 at 76,894,352 bp
  • A to G, chromosome 2 at 132,381,184 bp
  • C to G, chromosome 3 at 158,135,244 bp
  • T to A, chromosome 4 at 43,931,015 bp
  • T to A, chromosome 4 at 59,960,702 bp
  • T to C, chromosome 4 at 108,929,443 bp
  • T to C, chromosome 4 at 128,588,264 bp
  • T to C, chromosome 5 at 21,250,727 bp
  • T to C, chromosome 5 at 105,094,092 bp
  • A to G, chromosome 5 at 119,841,859 bp
  • A to T, chromosome 6 at 38,041,166 bp
  • A to G, chromosome 7 at 143,837,892 bp
  • G to A, chromosome 8 at 23,119,399 bp
  • C to A, chromosome 8 at 106,545,069 bp
  • T to C, chromosome 9 at 35,564,069 bp
  • T to C, chromosome 9 at 65,794,897 bp
  • T to C, chromosome 10 at 19,609,645 bp
  • T to A, chromosome 10 at 41,501,799 bp
  • C to G, chromosome 10 at 79,991,399 bp
  • A to T, chromosome 11 at 67,286,239 bp
  • A to T, chromosome 11 at 72,850,785 bp
  • A to T, chromosome 11 at 83,028,930 bp
  • TCATTGTCC to TCATTGTCCATTGTCC, chromosome 13 at 47,064,088 bp
  • C to A, chromosome 13 at 74,277,661 bp
  • T to C, chromosome 13 at 94,174,546 bp
  • T to A, chromosome 14 at 41,172,442 bp
  • C to A, chromosome 15 at 48,314,051 bp
  • T to C, chromosome 15 at 83,129,268 bp
  • A to G, chromosome 15 at 88,965,437 bp
  • C to T, chromosome 15 at 101,846,187 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2302 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040301-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.