Strain Name:
C57BL/6J-MtgxR2321Btlr/Mmmh
Stock Number:
040313-MU
Citation ID:
RRID:MMRRC_040313-MU
Other Names:
R2321 (G1), C57BL/6J-MtgxR2321Btlr
Major Collection:

Gene Information

Adcy9
Name: adenylate cyclase 9
Synonyms: ACtp10, D16Wsu65e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 11515
HGNC: HGNC:240
Homologene: 868
Tm9sf4
Name: transmembrane 9 superfamily protein member 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99237
Homologene: 21620
Senp6
Name: SUMO/sentrin specific peptidase 6
Synonyms: 2810017C20Rik, E130319N12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 215351
Homologene: 9196
Dcaf1
Name: DDB1 and CUL4 associated factor 1
Synonyms: B930007L02Rik, Vprbp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 321006
Homologene: 8805
Tasp1
Name: taspase, threonine aspartase 1
Synonyms: 4930485D02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 75812
Homologene: 9795
Rnps1
Name: RNA binding protein with serine rich domain 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 19826
VEGA: 17
Homologene: 40648
Zgrf1
Name: zinc finger, GRF-type containing 1
Synonyms: 4930422G04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 71643
Homologene: 34708
Tet2
Name: tet methylcytosine dioxygenase 2
Synonyms: E130014J05Rik, Ayu17-449
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 214133
Homologene: 49498
Rbm26
Name: RNA binding motif protein 26
Synonyms: C230097K14Rik, 1700009P03Rik, Pro1777
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 74213
Homologene: 41468
Fbxo32
Name: F-box protein 32
Synonyms: atrogin-1, MAFbx, ATROGIN1, 4833442G10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 67731
VEGA: 15
Homologene: 12182
Ppp1r2
Name: protein phosphatase 1, regulatory inhibitor subunit 2
Synonyms: 2310007G06Rik, 4930440J04Rik, 5430408E15Rik, I-2, 0610025N14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 66849
HGNC: HGNC:9288
Homologene: 4546
Ncam1
Name: neural cell adhesion molecule 1
Synonyms: NCAM-120, NCAM-140, NCAM, NCAM-1, E-NCAM, CD56, NCAM-180
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 17967
HGNC: HGNC:7656
Homologene: 40754
Clca4b
Name: chloride channel accessory 4B
Synonyms: AI747448
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 99709
HGNC: HGNC:2018
Homologene: 40808
Abcf2
Name: ATP-binding cassette, sub-family F (GCN20), member 2
Synonyms: 0710005O05Rik, Drr3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 27407
HGNC: HGNC:71
Homologene: 21408
Cpq
Name: carboxypeptidase Q
Synonyms: 2610034C17Rik, Pgcp, Lal-1, HLS2, 1190003P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 54381
VEGA: 15
Homologene: 9401
Tmem131l
Name: transmembrane 131 like
Synonyms: D930015E06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229473
Homologene: 9057
Slamf9
Name: SLAM family member 9
Synonyms: SF2001, Cd2f10, 2310026I04Rik, CD84-H1, CD2F-10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98365
Homologene: 12642
Dnajc12
Name: DnaJ heat shock protein family (Hsp40) member C12
Synonyms: Jdp1, mJDP1, J domain protein 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 30045
Homologene: 8492
Reln
Name: reelin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 19699
HGNC: HGNC:9957
Homologene: 3699
Myh15
Name: myosin, heavy chain 15
Synonyms: EG667772
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 667772
VEGA: 16
Homologene: 18929
Enpep
Name: glutamyl aminopeptidase
Synonyms: Ly51, Ly-51, Bp-1/6C3, APA, aminopeptidase-A, 6030431M22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 13809
HGNC: HGNC:3355
Homologene: 68216
Plekhg4
Name: pleckstrin homology domain containing, family G (with RhoGef domain) member 4
Synonyms: 4931414L13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 102075
Homologene: 18516
3110009E18Rik
Name: RIKEN cDNA 3110009E18 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 73103
Homologene: 41754
Uroc1
Name: urocanase domain containing 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243537
Homologene: 76629
Wdr64
Name: WD repeat domain 64
Synonyms: 4930415O10Rik, 4930511H01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 75820
Homologene: 51634
A2ml1
Name: alpha-2-macroglobulin like 1
Synonyms: BC048546, Ovos2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232400
Homologene: 45969
Serpinh1
Name: serine (or cysteine) peptidase inhibitor, clade H, member 1
Synonyms: Serpinh2, Cbp1, Cbp2, J6, gp46, colligin, Hsp47
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 12406
HGNC: HGNC:1546
Homologene: 20331
Pnmal2
Name: PNMA-like 2
Synonyms: EG434128
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 434128
Homologene: 35491
Crybg2
Name: crystallin beta-gamma domain containing 2
Synonyms: Aim1l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230806
Homologene: 19232
Otol1
Name: otolin 1
Synonyms: Gm414, LOC229389
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229389
Homologene: 19018
Slc26a4
Name: solute carrier family 26, member 4
Synonyms: Pds, pendrin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 23985
VEGA: 12
HGNC: HGNC:8818
Homologene: 20132
Rad51ap2
Name: RAD51 associated protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 209550
VEGA: 12
Homologene: 85245
Arhgef40
Name: Rho guanine nucleotide exchange factor (GEF) 40
Synonyms: E130112L23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 268739
Homologene: 19560
Olfr1389
Name: olfactory receptor 1389
Synonyms: GA_x6K02T2QP88-6004387-6003452, MOR256-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 259069
Homologene: 86690
Men1
Name: multiple endocrine neoplasia 1
Synonyms: menin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 17283
HGNC: HGNC:7010
Homologene: 7418
Slc22a13
Name: solute carrier family 22 (organic cation transporter), member 13
Synonyms: ORCTL3, OCTL1, OCTL3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 102570
VEGA: 9
HGNC: HGNC:8494
Homologene: 3140
Bhlha15
Name: basic helix-loop-helix family, member a15
Synonyms: 1810009C13Rik, Bhlhb8, Mist1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 17341
Homologene: 7838
Tmem71
Name: transmembrane protein 71
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 213068
VEGA: 15
Homologene: 51638
Krtap17-1
Name: keratin associated protein 17-1
Synonyms: A030006P16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 77914
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 120,160,596 bp
  • G to T, chromosome 1 at 172,477,413 bp
  • A to C, chromosome 1 at 175,795,087 bp
  • A to G, chromosome 2 at 140,057,412 bp
  • T to A, chromosome 2 at 153,204,586 bp
  • T to A, chromosome 3 at 70,018,525 bp
  • T to A, chromosome 3 at 83,936,023 bp
  • C to A, chromosome 3 at 127,562,407 bp
  • A to T, chromosome 3 at 129,309,147 bp
  • G to T, chromosome 3 at 129,309,148 bp
  • T to C, chromosome 3 at 133,486,339 bp
  • C to A, chromosome 3 at 144,932,373 bp
  • A to G, chromosome 4 at 134,074,511 bp
  • T to C, chromosome 5 at 21,915,020 bp
  • A to T, chromosome 5 at 24,567,253 bp
  • T to C, chromosome 5 at 144,191,196 bp
  • A to G, chromosome 6 at 90,347,247 bp
  • C to T, chromosome 6 at 128,580,386 bp
  • G to T, chromosome 7 at 16,945,565 bp
  • T to C, chromosome 7 at 99,346,385 bp
  • T to C, chromosome 8 at 105,377,540 bp
  • A to C, chromosome 9 at 49,544,832 bp
  • A to C, chromosome 9 at 80,123,740 bp
  • T to C, chromosome 9 at 106,838,473 bp
  • A to T, chromosome 9 at 119,195,628 bp
  • G to T, chromosome 10 at 63,407,211 bp
  • A to G, chromosome 11 at 49,431,280 bp
  • T to C, chromosome 11 at 99,993,920 bp
  • G to A, chromosome 12 at 11,457,057 bp
  • A to G, chromosome 12 at 31,540,544 bp
  • T to C, chromosome 14 at 51,994,276 bp
  • T to C, chromosome 14 at 105,153,427 bp
  • A to G, chromosome 15 at 33,594,145 bp
  • T to C, chromosome 15 at 58,191,293 bp
  • T to G, chromosome 15 at 66,552,000 bp
  • A to G, chromosome 16 at 4,288,268 bp
  • A to T, chromosome 16 at 31,265,303 bp
  • T to A, chromosome 16 at 49,113,073 bp
  • T to A, chromosome 17 at 24,422,168 bp
  • A to G, chromosome 19 at 6,339,838 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2321 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040313-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.