Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR2356Btlr/Mmmh
Stock Number:
040338-MU
Citation ID:
RRID:MMRRC_040338-MU
Other Names:
R2356 (G1), C57BL/6J-MtgxR2356Btlr
Major Collection:

Strain Information

Itga8
Name: integrin alpha 8
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 241226
HGNC: HGNC:6144
Homologene: 37396
Apoa5
Name: apolipoprotein A-V
Synonyms: RAP3, Apoav, 1300007O05Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 66113
Homologene: 14197
Erbb4
Name: erb-b2 receptor tyrosine kinase 4
Synonyms: ErbB4, Her4
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13869
HGNC: HGNC:3432
Homologene: 21084
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Matk
Name: megakaryocyte-associated tyrosine kinase
Synonyms: Ntk, HYL, CHK, Csk homologous kinase
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 17179
HGNC: HGNC:6906
Homologene: 48104
Cxcr4
Name: C-X-C motif chemokine receptor 4
Synonyms: PB-CKR, fusin, CD184, Cmkar4, Sdf1r, b2b220Clo
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12767
HGNC: HGNC:2561
Homologene: 20739
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 9,930,801 bp
  • A to T, chromosome 1 at 34,559,903 bp
  • T to A, chromosome 1 at 68,078,596 bp
  • A to G, chromosome 1 at 128,589,514 bp
  • A to T, chromosome 1 at 135,406,817 bp
  • T to C, chromosome 2 at 12,200,141 bp
  • A to T, chromosome 2 at 61,800,007 bp
  • A to G, chromosome 2 at 72,435,216 bp
  • C to A, chromosome 2 at 167,512,636 bp
  • T to C, chromosome 3 at 95,894,232 bp
  • A to G, chromosome 3 at 137,937,749 bp
  • T to A, chromosome 4 at 15,970,863 bp
  • A to G, chromosome 4 at 83,460,148 bp
  • C to T, chromosome 4 at 105,039,721 bp
  • C to A, chromosome 4 at 115,344,261 bp
  • T to G, chromosome 4 at 156,222,701 bp
  • G to T, chromosome 5 at 28,166,332 bp
  • C to T, chromosome 5 at 31,088,594 bp
  • G to T, chromosome 5 at 124,821,211 bp
  • A to G, chromosome 5 at 142,455,409 bp
  • C to A, chromosome 5 at 145,862,521 bp
  • G to A, chromosome 5 at 150,471,432 bp
  • G to A, chromosome 6 at 42,291,625 bp
  • A to G, chromosome 6 at 85,953,365 bp
  • A to G, chromosome 7 at 17,972,316 bp
  • C to T, chromosome 7 at 27,507,859 bp
  • A to T, chromosome 7 at 45,518,628 bp
  • G to A, chromosome 7 at 55,932,966 bp
  • G to T, chromosome 7 at 104,636,627 bp
  • T to G, chromosome 7 at 127,591,576 bp
  • T to A, chromosome 7 at 128,766,286 bp
  • A to T, chromosome 8 at 23,085,672 bp
  • G to A, chromosome 8 at 105,015,938 bp
  • T to A, chromosome 8 at 119,588,287 bp
  • T to C, chromosome 8 at 123,229,169 bp
  • C to A, chromosome 9 at 22,243,040 bp
  • T to A, chromosome 9 at 46,270,043 bp
  • T to C, chromosome 9 at 66,414,406 bp
  • T to A, chromosome 9 at 107,917,870 bp
  • A to G, chromosome 9 at 124,349,306 bp
  • G to A, chromosome 10 at 81,261,543 bp
  • A to G, chromosome 10 at 81,492,922 bp
  • A to G, chromosome 10 at 82,283,955 bp
  • T to C, chromosome 10 at 114,401,516 bp
  • T to C, chromosome 10 at 129,093,892 bp
  • A to T, chromosome 11 at 49,664,362 bp
  • A to T, chromosome 11 at 53,466,447 bp
  • T to C, chromosome 11 at 65,010,025 bp
  • T to C, chromosome 11 at 68,492,917 bp
  • T to A, chromosome 11 at 69,904,520 bp
  • T to A, chromosome 11 at 80,428,704 bp
  • G to C, chromosome 11 at 87,085,728 bp
  • A to G, chromosome 11 at 98,919,292 bp
  • T to C, chromosome 12 at 24,653,583 bp
  • C to T, chromosome 12 at 52,519,147 bp
  • A to G, chromosome 12 at 69,198,218 bp
  • A to C, chromosome 12 at 72,652,381 bp
  • G to T, chromosome 12 at 104,217,367 bp
  • T to C, chromosome 12 at 111,109,792 bp
  • G to C, chromosome 13 at 21,291,368 bp
  • A to T, chromosome 13 at 22,883,950 bp
  • A to T, chromosome 13 at 49,039,168 bp
  • A to G, chromosome 13 at 67,390,627 bp
  • A to T, chromosome 14 at 20,710,634 bp
  • A to G, chromosome 14 at 24,170,428 bp
  • C to T, chromosome 14 at 30,890,634 bp
  • T to C, chromosome 14 at 49,016,281 bp
  • T to C, chromosome 14 at 101,886,945 bp
  • C to T, chromosome 15 at 44,533,019 bp
  • A to T, chromosome 15 at 98,597,016 bp
  • T to A, chromosome 16 at 15,684,204 bp
  • G to T, chromosome 16 at 20,560,499 bp
  • A to T, chromosome 16 at 29,281,069 bp
  • A to T, chromosome 16 at 89,487,901 bp
  • G to T, chromosome 16 at 89,487,902 bp
  • G to A, chromosome 16 at 93,590,983 bp
  • T to C, chromosome 17 at 26,543,404 bp
  • A to G, chromosome 17 at 35,621,671 bp
  • T to A, chromosome 17 at 65,833,050 bp
  • T to A, chromosome 17 at 67,810,114 bp
  • A to T, chromosome 18 at 11,084,310 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2356 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040338-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.