Strain Name:
C57BL/6J-MtgxR2475Btlr/Mmmh
Stock Number:
040406-MU
Citation ID:
RRID:MMRRC_040406-MU
Other Names:
R2475 (G1), C57BL/6J-MtgxR2475Btlr
Major Collection:

Gene Information

Atic
Name: 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase
Synonyms: 2610509C24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 108147
HGNC: HGNC:794
Homologene: 2983
Trpc2
Name: transient receptor potential cation channel, subfamily C, member 2
Synonyms: TRPC2b, TRPC2a, mTrp2, trp2, Trrp2, 3010009O07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22064
Homologene: 135989
Actn4
Name: actinin alpha 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 60595
HGNC: HGNC:166
Homologene: 55857
Bbx
Name: bobby sox HMG box containing
Synonyms: 5730403O13Rik, 5530401J07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 70508
Homologene: 10634
Secisbp2l
Name: SECIS binding protein 2-like
Synonyms: 3110001I20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 70354
Homologene: 18923
Fntb
Name: farnesyltransferase, CAAX box, beta
Synonyms: 2010013E13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 110606
HGNC: HGNC:3785
Homologene: 1535
Lingo1
Name: leucine rich repeat and Ig domain containing 1
Synonyms: UNQ201, LERN1, 4930471K13Rik, LINGO-1, Lrrn6a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235402
Homologene: 41891
Nipbl
Name: NIPBL cohesin loading factor
Synonyms: 4921518A06Rik, 4933421G18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 71175
VEGA: 15
Homologene: 15850
Wrnip1
Name: Werner helicase interacting protein 1
Synonyms: WHIP, 4833444L21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 78903
Homologene: 10592
Mavs
Name: mitochondrial antiviral signaling protein
Synonyms: IPS-1, D430028G21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228607
Homologene: 17004
Aldh1a1
Name: aldehyde dehydrogenase family 1, subfamily A1
Synonyms: ALDH1, E1, Ahd-2, Ahd2, Raldh1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 11668
HGNC: HGNC:402
Homologene: 110441
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Atp7b
Name: ATPase, Cu++ transporting, beta polypeptide
Synonyms: WND, Wilson protein, Atp7a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11979
HGNC: HGNC:870
Homologene: 20063
Trpm8
Name: transient receptor potential cation channel, subfamily M, member 8
Synonyms: CMR1, TRPP8, Trp-p8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 171382
Homologene: 23433
Phf19
Name: PHD finger protein 19
Synonyms: 3110009G19Rik, 3321402G02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74016
Homologene: 12524
Inpp4b
Name: inositol polyphosphate-4-phosphatase, type II
Synonyms: E130107I17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234515
HGNC: HGNC:6075
Homologene: 20832
Rufy4
Name: RUN and FYVE domain containing 4
Synonyms: F930048N03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 435626
Homologene: 52359
Pramel24
Name: PRAME like 24
Synonyms: Gm13078
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 277666
Homologene: 133722
Grip1
Name: glutamate receptor interacting protein 1
Synonyms: 4931400F03Rik, eb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 74053
Homologene: 12938
Man2b2
Name: mannosidase 2, alpha B2
Synonyms: 135 kDa alpha-D-mannosidase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 17160
Homologene: 7411
AC238840.3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
4930438A08Rik
Name: RIKEN cDNA 4930438A08 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 73988
Homologene: 104884
Ccdc28b
Name: coiled coil domain containing 28B
Synonyms: 1110002E08Rik, 1810010N17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 66264
Homologene: 11509
Olfr112
Name: olfactory receptor 112
Synonyms: MOR218-4, GA_x6K02T2PSCP-2023769-2022807, 4930580F03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 258096
Gpsm3
Name: G-protein signalling modulator 3 (AGS3-like, C. elegans)
Synonyms: AGS4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 106512
Homologene: 11136
Gm5862
Name: predicted gene 5862
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 545739
Homologene: 69402
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 71,559,269 bp
  • T to C, chromosome 1 at 74,147,663 bp
  • A to G, chromosome 1 at 88,354,449 bp
  • C to T, chromosome 2 at 34,895,795 bp
  • A to T, chromosome 2 at 76,811,243 bp
  • A to G, chromosome 2 at 76,872,750 bp
  • C to T, chromosome 2 at 125,740,737 bp
  • G to T, chromosome 2 at 131,240,450 bp
  • A to T, chromosome 4 at 129,620,652 bp
  • T to A, chromosome 4 at 143,726,825 bp
  • G to A, chromosome 5 at 26,019,492 bp
  • A to G, chromosome 5 at 36,807,875 bp
  • G to A, chromosome 7 at 28,898,266 bp
  • T to A, chromosome 7 at 38,867,834 bp
  • C to T, chromosome 7 at 102,086,386 bp
  • C to T, chromosome 8 at 21,994,776 bp
  • T to A, chromosome 8 at 82,041,978 bp
  • T to A, chromosome 9 at 56,619,626 bp
  • T to C, chromosome 10 at 119,978,496 bp
  • A to G, chromosome 11 at 58,287,496 bp
  • C to A, chromosome 12 at 76,887,888 bp
  • G to A, chromosome 13 at 32,806,958 bp
  • A to T, chromosome 15 at 8,335,006 bp
  • C to A, chromosome 16 at 50,220,519 bp
  • A to G, chromosome 17 at 34,590,560 bp
  • A to G, chromosome 17 at 37,563,711 bp
  • T to A, chromosome 19 at 20,640,078 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2475 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040406-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.