Strain Name:
C57BL/6J-MtgxR2845Btlr/Mmmh
Stock Number:
040438-MU
Citation ID:
RRID:MMRRC_040438-MU
Other Names:
R2845 (G1), C57BL/6J-MtgxR2845Btlr
Major Collection:

Strain Information

Rfx3
Name: regulatory factor X, 3 (influences HLA class II expression)
Synonyms: MRFX3, C230093O12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 19726
HGNC: HGNC:9984
Homologene: 7917
Plekha1
Name: pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 1
Synonyms: TAPP1, C920009D07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 101476
Homologene: 11001
Mark2
Name: MAP/microtubule affinity regulating kinase 2
Synonyms: Par-1, Emk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 13728
HGNC: HGNC:3332
Homologene: 69013
Zfp407
Name: zinc finger protein 407
Synonyms: LOC381139, LOC240469, 6430585N13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 240476
Homologene: 86402
Fntb
Name: farnesyltransferase, CAAX box, beta
Synonyms: 2010013E13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 110606
Homologene: 1535
Sbf1
Name: SET binding factor 1
Synonyms: 2610510A08Rik, Mtmr5, B230113C15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 77980
Homologene: 84710
Zbtb8os
Name: zinc finger and BTB domain containing 8 opposite strand
Synonyms: 2310028N13Rik, 2010001H09Rik, Arch
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 67106
Homologene: 12083
Csnk1g2
Name: casein kinase 1, gamma 2
Synonyms: 2810429I12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 103236
VEGA: 10
HGNC: HGNC:2455
Homologene: 100845
Cep152
Name: centrosomal protein 152
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 99100
Homologene: 37159
Slc15a4
Name: solute carrier family 15, member 4
Synonyms: C130069N12Rik, PTR4, PHT1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100561
Homologene: 26432
Armh4
Name: armadillo-like helical domain containing 4
Synonyms: 3632451O06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 67419
Homologene: 12127
Fhad1
Name: forkhead-associated (FHA) phosphopeptide binding domain 1
Synonyms: 2900090M10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 329977
Homologene: 77947
Cplane1
Name: ciliogenesis and planar polarity effector 1
Synonyms: b2b012Clo, Jbts17, Hug, 2410089E03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 73692
Homologene: 11315
Efcab7
Name: EF-hand calcium binding domain 7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230500
Homologene: 19952
Ccdc88b
Name: coiled-coil domain containing 88B
Synonyms: 2610041P08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 78317
VEGA: 19
Homologene: 49992
Col19a1
Name: collagen, type XIX, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12823
HGNC: HGNC:2196
Homologene: 55608
Adamts7
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 7
Synonyms: ADAM-TS7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 108153
HGNC: HGNC:223
Homologene: 22803
Skint10
Name: selection and upkeep of intraepithelial T cells 10
Synonyms: A030001H23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230613
Homologene: 136292
Pign
Name: phosphatidylinositol glycan anchor biosynthesis, class N
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 27392
HGNC: HGNC:8967
Homologene: 6330
Frem3
Name: Fras1 related extracellular matrix protein 3
Synonyms: LOC333315
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 333315
Homologene: 35388
Or10al3
Name: olfactory receptor family 10 subfamily AL member 3
Synonyms: GA_x6K02T2PSCP-2159633-2160598, MOR263-7, Olfr119
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 258095
Homologene: 17195
Dgkz
Name: diacylglycerol kinase zeta
Synonyms: mDGK[z], E130307B02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 104418
HGNC: HGNC:2857
Homologene: 37831
Hsd3b1
Name: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
Synonyms: D3Ertd383e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 15492
Homologene: 69149
Ptpru
Name: protein tyrosine phosphatase, receptor type, U
Synonyms: Ptprl, RPTPlambda
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 19273
HGNC: HGNC:9683
Homologene: 4168
Ssh3
Name: slingshot protein phosphatase 3
Synonyms: SSH-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 245857
VEGA: 19
Homologene: 32372
Cherp
Name: calcium homeostasis endoplasmic reticulum protein
Synonyms: 5730408I11Rik, SCAF6, DAN16, D8Wsu96e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 27967
Homologene: 4656
Arhgap25
Name: Rho GTPase activating protein 25
Synonyms: A130039I20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232201
Homologene: 45507
Tgfbr3l
Name: transforming growth factor, beta receptor III-like
Synonyms: LOC100039590, Gm14378
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 100044509
Homologene: 130375
Tas2r138
Name: taste receptor, type 2, member 138
Synonyms: mt2r31, Tas2r38, T2R138
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 387513
HGNC: HGNC:9584
Homologene: 47976
Gpx6
Name: glutathione peroxidase 6
Synonyms: olfactory, 1700020G18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 75512
HGNC: HGNC:4558
Homologene: 130008
Bahd1
Name: bromo adjacent homology domain containing 1
Synonyms: LOC228536
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228536
Homologene: 8976
Zfp24
Name: zinc finger protein 24
Synonyms: KOX17, ZF-12, 3526401F17Rik, 5033419P20Rik, Zfp191
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 59057
Homologene: 5068
Mrgpra2a
Name: MAS-related GPR, member A2A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 668727
Homologene: 79615
Atg4a
Name: autophagy related 4A, cysteine peptidase
Synonyms: autophagin 2, Autl2, Apg4a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 666468
Homologene: 70873
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 24,559,681 bp
  • C to A, chromosome 1 at 105,657,796 bp
  • A to G, chromosome 2 at 91,940,686 bp
  • G to A, chromosome 2 at 118,922,523 bp
  • A to G, chromosome 2 at 125,587,974 bp
  • T to C, chromosome 3 at 98,852,778 bp
  • T to A, chromosome 4 at 99,909,638 bp
  • T to C, chromosome 4 at 112,715,826 bp
  • A to T, chromosome 4 at 129,341,516 bp
  • T to A, chromosome 4 at 131,819,661 bp
  • G to T, chromosome 4 at 141,904,968 bp
  • G to A, chromosome 4 at 143,654,298 bp
  • A to G, chromosome 5 at 127,604,536 bp
  • T to C, chromosome 6 at 40,612,767 bp
  • C to T, chromosome 6 at 87,459,967 bp
  • T to A, chromosome 7 at 47,427,130 bp
  • G to T, chromosome 7 at 130,908,365 bp
  • A to G, chromosome 8 at 4,249,280 bp
  • C to A, chromosome 8 at 72,466,403 bp
  • T to C, chromosome 8 at 80,613,220 bp
  • T to A, chromosome 9 at 90,174,464 bp
  • A to G, chromosome 10 at 80,638,604 bp
  • C to A, chromosome 12 at 76,887,888 bp
  • A to T, chromosome 13 at 21,318,875 bp
  • T to C, chromosome 14 at 49,772,855 bp
  • G to A, chromosome 15 at 8,216,380 bp
  • A to G, chromosome 15 at 89,303,218 bp
  • T to C, chromosome 17 at 37,700,823 bp
  • G to T, chromosome 18 at 24,017,828 bp
  • G to T, chromosome 18 at 84,558,397 bp
  • T to C, chromosome 19 at 4,265,296 bp
  • T to G, chromosome 19 at 6,854,465 bp
  • T to C, chromosome 19 at 7,286,862 bp
  • T to C, chromosome 19 at 27,806,786 bp
  • G to A, chromosome X at 140,992,840 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2845 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040438-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.