Strain Name:
C57BL/6J-MtgxR2887Btlr/Mmmh
Stock Number:
040475-MU
Citation ID:
RRID:MMRRC_040475-MU
Other Names:
R2887 (G1), C57BL/6J-MtgxR2887Btlr
Major Collection:

Gene Information

Adamts20
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 20
Synonyms: ADAMTS-20, bt
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223838
Homologene: 11808
Tcf7l1
Name: transcription factor 7 like 1 (T cell specific, HMG box)
Synonyms: Tcf3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 21415
Homologene: 7563
Aurka
Name: aurora kinase A
Synonyms: Ayk1, Ark1, IAK, AIRK1, aurora A, Aurora-A, Stk6, IAK1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20878
Homologene: 2670
Eefsec
Name: eukaryotic elongation factor, selenocysteine-tRNA-specific
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 65967
Homologene: 11073
Clock
Name: circadian locomotor output cycles kaput
Synonyms: 5330400M04Rik, KAT13D, bHLHe8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 12753
HGNC: HGNC:2082
Homologene: 3603
Ptprc
Name: protein tyrosine phosphatase, receptor type, C
Synonyms: T200, B220, CD45, Lyt-4, Ly-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 19264
HGNC: HGNC:9666
Homologene: 2126
Serpina3c
Name: serine (or cysteine) peptidase inhibitor, clade A, member 3C
Synonyms: Kalbp, 1A1, Klkbp, alpha-1 antiproteinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 16625
HGNC: HGNC:16
Homologene: 111129
Clec7a
Name: C-type lectin domain family 7, member a
Synonyms: dectin-1, BGR, beta-GR, beta-glucan receptor, Clecsf12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 56644
Homologene: 49606
Golm1
Name: golgi membrane protein 1
Synonyms: D030064E01Rik, PSEC0257, GP73, 2310001L02Rik, Golph2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 105348
VEGA: 13
Homologene: 12346
Ppp1r3a
Name: protein phosphatase 1, regulatory subunit 3A
Synonyms: GM, RGL
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 140491
HGNC: HGNC:9291
Homologene: 48124
Lama4
Name: laminin, alpha 4
Synonyms: laminin [a]4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 16775
HGNC: HGNC:6484
Homologene: 37604
Zfp14
Name: zinc finger protein 14
Synonyms: Krox-9, Zfp-14, 4732429I09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 243906
Homologene: 45619
Lamp1
Name: lysosomal-associated membrane protein 1
Synonyms: CD107a, Lamp-1, Perk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 16783
HGNC: HGNC:6499
Homologene: 4061
Vmn2r98
Name: vomeronasal 2, receptor 98
Synonyms: EG224552
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224552
Homologene: 115024
Slc7a13
Name: solute carrier family 7, (cationic amino acid transporter, y+ system) member 13
Synonyms: AGT1, XAT2, AGT-1, 0610009O04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 74087
Homologene: 23656
Rimklb
Name: ribosomal modification protein rimK-like family member B
Synonyms: 4931417E21Rik, 4933426K21Rik, NAAGS
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 108653
Homologene: 18968
Ivl
Name: involucrin
Synonyms: 1110019C06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 16447
HGNC: HGNC:6187
Homologene: 137207
Fibin
Name: fin bud initiation factor homolog (zebrafish)
Synonyms: Fibin, 1110018M03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 67606
Homologene: 12163
1810009A15Rik
Name: RIKEN cDNA 1810009A15 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 66276
Homologene: 84408
Or13a28
Name: olfactory receptor family 13 subfamily A member 28
Synonyms: IH7, MOR253-1, GA_x6K02T2PBJ9-42783826-42784758, Olfr61
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 18362
Homologene: 79374
Slc35a5
Name: solute carrier family 35, member A5
Synonyms: D16Ertd450e, 1010001J06Rik, D730043G07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 74102
Homologene: 9930
Emg1
Name: EMG1 N1-specific pseudouridine methyltransferase
Synonyms: Grcc2f
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14791
Homologene: 4617
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 138,080,178 bp
  • T to A, chromosome 2 at 110,362,777 bp
  • A to G, chromosome 2 at 172,367,120 bp
  • C to A, chromosome 3 at 92,571,392 bp
  • A to G, chromosome 4 at 19,819,052 bp
  • G to A, chromosome 5 at 76,245,273 bp
  • T to G, chromosome 6 at 14,718,249 bp
  • G to A, chromosome 6 at 72,632,088 bp
  • T to C, chromosome 6 at 88,258,359 bp
  • T to C, chromosome 6 at 122,472,698 bp
  • T to C, chromosome 6 at 124,705,063 bp
  • C to T, chromosome 6 at 129,470,997 bp
  • G to T, chromosome 7 at 30,038,765 bp
  • A to T, chromosome 7 at 140,638,225 bp
  • T to A, chromosome 8 at 13,173,891 bp
  • A to C, chromosome 10 at 39,092,254 bp
  • T to C, chromosome 12 at 104,147,290 bp
  • G to T, chromosome 13 at 59,640,230 bp
  • C to T, chromosome 15 at 94,330,578 bp
  • A to G, chromosome 16 at 45,151,560 bp
  • G to T, chromosome 17 at 19,081,177 bp
  • T to C, chromosome 19 at 8,890,031 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R2887 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040475-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.