Strain Name:
C57BL/6J-MtgxR3014Btlr/Mmmh
Stock Number:
040535-MU
Citation ID:
RRID:MMRRC_040535-MU
Other Names:
R3014 (G1), C57BL/6J-MtgxR3014Btlr
Major Collection:

Gene Information

Gria4
Name: glutamate receptor, ionotropic, AMPA4 (alpha 4)
Synonyms: Glur4, Gluralpha4, spkw1, Glur-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 14802
HGNC: HGNC:4574
Homologene: 20227
Gmps
Name: guanine monophosphate synthetase
Synonyms: Gm9479
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229363
HGNC: HGNC:4378
Homologene: 68367
Ptpn23
Name: protein tyrosine phosphatase, non-receptor type 23
Synonyms: PTP-TD14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 104831
Homologene: 135706
Rttn
Name: rotatin
Synonyms: 4921538A15Rik, C530033I08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 246102
VEGA: 18
Homologene: 65275
Tenm2
Name: teneurin transmembrane protein 2
Synonyms: 2610040L17Rik, Odz2, 9330187F13Rik, Ten-m2, D3Bwg1534e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 23964
Homologene: 22672
Tox
Name: thymocyte selection-associated high mobility group box
Synonyms: 1700007F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 252838
Homologene: 8822
Psmg1
Name: proteasome (prosome, macropain) assembly chaperone 1
Synonyms: Dscr2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 56088
HGNC: HGNC:3043
Homologene: 2759
Dmbt1
Name: deleted in malignant brain tumors 1
Synonyms: MUCLIN, gp300, Crpd, hensin, CRP-[a], ebnerin, vomeroglandin, CRP-[b]
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 12945
HGNC: HGNC:2926
Homologene: 68990
Grm7
Name: glutamate receptor, metabotropic 7
Synonyms: E130018M02Rik, mGluR7, Tg(SMN2)89Ahmb, 6330570A01Rik, SMN2, mGlu7a receptor, Gpr1g
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 108073
HGNC: HGNC:4599
Homologene: 20233
Srsf7
Name: serine and arginine-rich splicing factor 7
Synonyms: NX-96, 9G8, Sfrs7, 9430065L19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 225027
VEGA: 17
Homologene: 134446
Lama5
Name: laminin, alpha 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16776
HGNC: HGNC:6485
Homologene: 4060
Fam193a
Name: family with sequence homology 193, member A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231128
Homologene: 2746
Sv2a
Name: synaptic vesicle glycoprotein 2 a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 64051
Homologene: 32237
Hmces
Name: 5-hydroxymethylcytosine (hmC) binding, ES cell specific
Synonyms: Srap1, 8430410A17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232210
Homologene: 6636
Ppip5k2
Name: diphosphoinositol pentakisphosphate kinase 2
Synonyms: Hisppd1, Cfap160, Vip2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227399
Homologene: 49409
Dnah2
Name: dynein, axonemal, heavy chain 2
Synonyms: D330014H01Rik, Dnahc2, Dnhd3, 2900022L05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 327954
HGNC: HGNC:2948
Homologene: 72110
Map3k5
Name: mitogen-activated protein kinase kinase kinase 5
Synonyms: Mekk5, ASK1, 7420452D20Rik, ASK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 26408
HGNC: HGNC:6857
Homologene: 38114
Gm4981
Name: predicted gene 4981
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 245263
Homologene: 134545
Angpt4
Name: angiopoietin 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 11602
HGNC: HGNC:487
Homologene: 36302
Parp3
Name: poly (ADP-ribose) polymerase family, member 3
Synonyms: PARP-3, Adprt3, A930002C11Rik, Adprtl3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235587
HGNC: HGNC:273
Homologene: 4005
Gm5155
Name: predicted gene 5155
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 381852
Homologene: 115938
Myo1a
Name: myosin IA
Synonyms: Myhl, brush border myosin 1, BBM-I
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 432516
VEGA: 10
HGNC: HGNC:7595
Homologene: 21113
Vmn2r13
Name: vomeronasal 2, receptor 13
Synonyms: Gm4867
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231589
Homologene: 129606
Xkr6
Name: X-linked Kx blood group related 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 219149
Homologene: 18287
Olfr1347
Name: olfactory receptor 1347
Synonyms: MOR103-19_i, GA_x6K02T2QGBW-3218686-3217748, MOR103-11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258383
Homologene: 128065
Lypd10
Name: Ly6/PLAUR domain containing 10
Synonyms: BC049730
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 232972
Kcnd3
Name: potassium voltage-gated channel, Shal-related family, member 3
Synonyms: potassium channel Kv4.3L, potassium channel Kv4.3M, Kv4.3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 56543
HGNC: HGNC:6239
Homologene: 21036
Kng1
Name: kininogen 1
Synonyms: H-kininigen, L-kininogen
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 16644
HGNC: HGNC:6383
Homologene: 88343
Gprin1
Name: G protein-regulated inducer of neurite outgrowth 1
Synonyms: GRIN1, Z16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 26913
Homologene: 8072
Olfr1377
Name: olfactory receptor 1377
Synonyms: GA_x6K02T2QP88-4453480-4452557, MOR129-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258913
Homologene: 64929
Hcar2
Name: hydroxycarboxylic acid receptor 2
Synonyms: Pumag, HM74, PUMA-G, Gpr109b, Niacr1, Gpr109a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 80885
Homologene: 4391
Olfr303
Name: olfactory receptor 303
Synonyms: MOR104-2, GA_x6K02T2NHDJ-9712819-9713778
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258612
Homologene: 105157
Tas2r107
Name: taste receptor, type 2, member 107
Synonyms: T2R07, mGR06, Tas2r7, T2R4, STC 5-1, mt2r43
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 387342
Homologene: 137369
Olfr723
Name: olfactory receptor 723
Synonyms: MOR247-4, MOR247-3P, GA_x6K02T2PMLR-5600424-5599495
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 259147
Homologene: 71986
Gm10220
Name: predicted gene 10220
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 434689
Homologene: 69402
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 97,744,075 bp
  • G to T, chromosome 2 at 151,929,597 bp
  • A to G, chromosome 2 at 180,181,562 bp
  • A to G, chromosome 3 at 64,015,436 bp
  • G to A, chromosome 3 at 96,189,435 bp
  • C to T, chromosome 3 at 105,658,766 bp
  • A to G, chromosome 4 at 6,741,581 bp
  • G to C, chromosome 5 at 26,117,828 bp
  • A to T, chromosome 5 at 34,465,672 bp
  • T to A, chromosome 5 at 109,171,761 bp
  • A to T, chromosome 5 at 123,864,705 bp
  • T to G, chromosome 6 at 87,917,841 bp
  • G to T, chromosome 6 at 110,646,348 bp
  • A to G, chromosome 6 at 131,660,009 bp
  • A to T, chromosome 7 at 6,488,471 bp
  • A to G, chromosome 7 at 17,915,666 bp
  • A to G, chromosome 7 at 24,713,396 bp
  • A to G, chromosome 7 at 86,394,676 bp
  • A to G, chromosome 7 at 131,032,097 bp
  • A to G, chromosome 9 at 4,464,294 bp
  • C to A, chromosome 9 at 106,471,315 bp
  • T to C, chromosome 9 at 110,389,695 bp
  • A to T, chromosome 10 at 20,094,429 bp
  • G to A, chromosome 10 at 58,235,534 bp
  • T to G, chromosome 10 at 127,716,345 bp
  • T to C, chromosome 11 at 36,023,973 bp
  • A to G, chromosome 11 at 50,984,707 bp
  • A to T, chromosome 11 at 69,430,478 bp
  • T to A, chromosome 13 at 54,738,475 bp
  • G to A, chromosome 14 at 49,929,032 bp
  • T to C, chromosome 14 at 63,819,344 bp
  • C to T, chromosome 16 at 23,079,370 bp
  • T to A, chromosome 16 at 95,980,048 bp
  • T to C, chromosome 17 at 80,201,561 bp
  • G to C, chromosome 18 at 89,014,620 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3014 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040535-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.