Strain Name:
C57BL/6J-MtgxR3025Btlr/Mmmh
Stock Number:
040541-MU
Citation ID:
RRID:MMRRC_040541-MU
Other Names:
R3025 (G1), C57BL/6J-MtgxR3025Btlr
Major Collection:

Gene Information

Rock2
Name: Rho-associated coiled-coil containing protein kinase 2
Synonyms: Rock-II, B230113H15Rik, Rho-kinase, ROKalpha, Rock2m
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 19878
VEGA: 12
Homologene: 21010
Tars2
Name: threonyl-tRNA synthetase 2, mitochondrial (putative)
Synonyms: 2610024N01Rik, Tarsl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 71807
Homologene: 23520
Scaf4
Name: SR-related CTD-associated factor 4
Synonyms: Sra4, Sfrs15, Srsf15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 224432
VEGA: 16
Homologene: 16227
Homer1
Name: homer scaffolding protein 1
Synonyms: Ves-1, PSD-Zip45
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 26556
Homologene: 3155
Sec61a1
Name: Sec61 alpha 1 subunit (S. cerevisiae)
Synonyms: Sec61a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 53421
Homologene: 55537
Chd6
Name: chromodomain helicase DNA binding protein 6
Synonyms: 6330406J24Rik, 5430439G14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 71389
Homologene: 32772
Dnajc16
Name: DnaJ heat shock protein family (Hsp40) member C16
Synonyms: 2900037O03Rik, 4732437J24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 214063
Homologene: 45414
Cacna1a
Name: calcium channel, voltage-dependent, P/Q type, alpha 1A subunit
Synonyms: Cacnl1a4, alpha1A, Ccha1a, SCA6, nmf352, smrl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 12286
HGNC: HGNC:1388
Homologene: 56383
Akap6
Name: A kinase (PRKA) anchor protein 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 238161
VEGA: 12
HGNC: HGNC:376
Homologene: 3157
Msh4
Name: mutS homolog 4
Synonyms: mMsh4, 4930485C04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 55993
HGNC: HGNC:7327
Homologene: 1830
Abcg4
Name: ATP binding cassette subfamily G member 4
Synonyms: 6430517O04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 192663
Homologene: 75179
Rp1
Name: retinitis pigmentosa 1 (human)
Synonyms: oxygen-regulated protein 1, Orp1, mG145, Dcdc3, Rp1h
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 19888
Homologene: 4564
Map3k19
Name: mitogen-activated protein kinase kinase kinase 19
Synonyms: Ysk4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 22625
Homologene: 19318
Olfr228
Name: olfactory receptor 228
Synonyms: GA_x6K02T0101M-139-669, MOR189-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258400
Homologene: 17233
Kcnb2
Name: potassium voltage gated channel, Shab-related subfamily, member 2
Synonyms: Kv2.2, 9630047L19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98741
HGNC: HGNC:6232
Homologene: 31263
Ogfod1
Name: 2-oxoglutarate and iron-dependent oxygenase domain containing 1
Synonyms: 4930415J21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 270086
Homologene: 41238
Vmn2r106
Name: vomeronasal 2, receptor 106
Synonyms: EG224576
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224576
Homologene: 135824
Ace
Name: angiotensin I converting enzyme (peptidyl-dipeptidase A) 1
Synonyms: CD143
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 11421
HGNC: HGNC:2707
Homologene: 37351
Gm10842
Name: predicted gene 10842
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Atp13a2
Name: ATPase type 13A2
Synonyms: 1110012E06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 74772
Homologene: 56940
Olfr808
Name: olfactory receptor 808
Synonyms: GA_x6K02T2PULF-11446184-11447122, MOR112-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 258930
Homologene: 17438
Vmn1r57
Name: vomeronasal 1 receptor 57
Synonyms: Gm7519
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 665150
Homologene: 41799
Cptp
Name: ceramide-1-phosphate transfer protein
Synonyms: Gltpd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 79554
Homologene: 11550
Gm17509
Name: predicted gene, 17509
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 4,352,675 bp
  • C to A, chromosome 1 at 15,710,835 bp
  • T to C, chromosome 1 at 127,838,553 bp
  • A to T, chromosome 2 at 86,483,739 bp
  • GATCAT to GAT, chromosome 2 at 160,966,552 bp
  • C to T, chromosome 3 at 95,747,640 bp
  • T to A, chromosome 3 at 153,863,491 bp
  • C to T, chromosome 4 at 140,994,348 bp
  • C to A, chromosome 4 at 141,774,611 bp
  • T to C, chromosome 4 at 155,867,221 bp
  • A to T, chromosome 6 at 88,512,220 bp
  • A to T, chromosome 7 at 5,220,715 bp
  • T to A, chromosome 8 at 84,580,225 bp
  • A to T, chromosome 8 at 94,063,052 bp
  • A to G, chromosome 9 at 44,274,860 bp
  • T to A, chromosome 10 at 129,767,673 bp
  • T to C, chromosome 11 at 105,147,076 bp
  • T to A, chromosome 11 at 105,974,093 bp
  • A to G, chromosome 12 at 16,977,810 bp
  • A to G, chromosome 12 at 53,140,143 bp
  • C to T, chromosome 13 at 93,402,074 bp
  • T to C, chromosome 13 at 117,220,576 bp
  • A to T, chromosome 16 at 90,251,938 bp
  • A to G, chromosome 17 at 20,278,885 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3025 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040541-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.