Strain Name:
C57BL/6J-MtgxR3076Btlr/Mmmh
Stock Number:
040566-MU
Citation ID:
RRID:MMRRC_040566-MU
Other Names:
R3076 (G1), C57BL/6J-MtgxR3076Btlr
Major Collection:

Strain Information

Meis1
Name: Meis homeobox 1
Synonyms: C530044H18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17268
HGNC: HGNC:7000
Homologene: 86803
Magi1
Name: membrane associated guanylate kinase, WW and PDZ domain containing 1
Synonyms: WWP3, Gukmi1, AIP3, BAP1, Baiap1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14924
HGNC: HGNC:946
Homologene: 31257
Col18a1
Name: collagen, type XVIII, alpha 1
Synonyms: endostatin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 12822
HGNC: HGNC:2195
Homologene: 7673
Aopep
Name: aminopeptidase O
Synonyms: ApO, 2010111I01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 72061
HGNC: HGNC:1361
Homologene: 66273
Matk
Name: megakaryocyte-associated tyrosine kinase
Synonyms: Ntk, HYL, CHK, Csk homologous kinase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 17179
HGNC: HGNC:6906
Homologene: 48104
Cct8
Name: chaperonin containing Tcp1, subunit 8 (theta)
Synonyms: Cctq, Tcpq
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 12469
HGNC: HGNC:1623
Homologene: 4802
Nbr1
Name: NBR1, autophagy cargo receptor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17966
HGNC: HGNC:6746
Homologene: 7438
Ascc2
Name: activating signal cointegrator 1 complex subunit 2
Synonyms: 2610034L15Rik, ASC1p100, 1700011I11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 75452
Homologene: 41774
St7
Name: suppression of tumorigenicity 7
Synonyms: RAY1, HELG, SEN4, TSG7, Fam4a2, 9430001H04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 64213
Homologene: 10185
Nbeal2
Name: neurobeachin-like 2
Synonyms: 1110014F23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235627
Homologene: 86422
Mtpn
Name: myotrophin
Synonyms: V1, Gcdp, 5033418D15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14489
Homologene: 40607
Plaa
Name: phospholipase A2, activating protein
Synonyms: D4Ertd618e, Ufd3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 18786
HGNC: HGNC:9043
Homologene: 3138
Mnt
Name: max binding protein
Synonyms: Rox, bHLHd3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17428
HGNC: HGNC:7188
Homologene: 7842
Svil
Name: supervillin
Synonyms: B430302E16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225115
Homologene: 25090
Amigo2
Name: adhesion molecule with Ig like domain 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 105827
VEGA: 15
Homologene: 18649
Khdc3
Name: KH domain containing 3, subcortical maternal complex member
Synonyms: ecat1, FILIA, 2410004A20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 66991
Homologene: 49842
Adgrd1
Name: adhesion G protein-coupled receptor D1
Synonyms: E230012M21Rik, Gpr133
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 243277
Homologene: 34616
Shtn1
Name: shootin 1
Synonyms: shootin1, 4930506M07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 71653
VEGA: 19
Homologene: 41249
Med28
Name: mediator complex subunit 28
Synonyms: Eg1, 1500003D12Rik, magicin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 66999
Homologene: 11873
Uggt2
Name: UDP-glucose glycoprotein glucosyltransferase 2
Synonyms: 3110001A05Rik, 1810064L21Rik, 3110027P15Rik, A230065J02Rik, Ugcgl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 66435
Homologene: 56875
Nyap2
Name: neuronal tyrosine-phophorylated phosphoinositide 3-kinase adaptor 2
Synonyms: Jr6, 9430031J16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 241134
Homologene: 18239
Atp1a3
Name: ATPase, Na+/K+ transporting, alpha 3 polypeptide
Synonyms: Atpa-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 232975
HGNC: HGNC:801
Homologene: 113729
Ugcg
Name: UDP-glucose ceramide glucosyltransferase
Synonyms: GlcT-1, Epcs21, Ugcgl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 22234
Homologene: 37763
Dock3
Name: dedicator of cyto-kinesis 3
Synonyms: PBP, Moca
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 208869
HGNC: HGNC:2989
Homologene: 21030
Ltbp2
Name: latent transforming growth factor beta binding protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 16997
HGNC: HGNC:6715
Homologene: 369
Clstn3
Name: calsyntenin 3
Synonyms: Cst-3, CSTN3, alcadein-beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232370
Homologene: 22836
Cabcoco1
Name: ciliary associated calcium binding coiled-coil 1
Synonyms: 1700040L02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 73287
Homologene: 12495
Npr2
Name: natriuretic peptide receptor 2
Synonyms: guanylyl cyclase-B, cn, pwe
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230103
HGNC: HGNC:7944
Homologene: 2970
Or1e29
Name: olfactory receptor family 1 subfamily E member 29
Synonyms: GA_x6K02T2P1NL-3932085-3931147, MOR135-6, Olfr389
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 259011
Homologene: 85925
Arfgef3
Name: ARFGEF family member 3
Synonyms: B930094H20Rik, D10Bwg1379e, BIG3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 215821
VEGA: 10
Homologene: 41366
Nrxn3
Name: neurexin III
Synonyms: neurexin III beta, neurexin III alpha, neurexin III beta, neurexin III alpha, 9330112C09Rik, D12Bwg0831e, 4933401A11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 18191
HGNC: HGNC:8010
Homologene: 83225
Kif14
Name: kinesin family member 14
Synonyms: N-3 kinesin, D1Ertd367e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 381293
Homologene: 8916
Or1o11
Name: olfactory receptor family 1 subfamily O member 11
Synonyms: MOR156-5, GA_x6K02T2PSCP-1893605-1894534, Olfr108
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 258457
Homologene: 44985
Phldb2
Name: pleckstrin homology like domain, family B, member 2
Synonyms: LL5beta, LL5b, C820004H04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 208177
Homologene: 17100
Sh2d2a
Name: SH2 domain containing 2A
Synonyms: TSAd, Lck-associated adapter protein, Lad, RIBP, Rlk/Itk-binding protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 27371
Homologene: 2958
Gtf3c4
Name: general transcription factor IIIC, polypeptide 4
Synonyms: KAT12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 269252
HGNC: HGNC:4667
Homologene: 8147
Ptprb
Name: protein tyrosine phosphatase, receptor type, B
Synonyms: 3230402H02Rik, VE-PTP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 19263
VEGA: 10
HGNC: HGNC:9665
Homologene: 2125
Dlgap3
Name: DLG associated protein 3
Synonyms: PSD-95/SAP90-binding protein 3, SAP90/PSD 95 associated protein 3, DAP3, Sapap3, Prpl8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242667
Homologene: 18276
Ces1b
Name: carboxylesterase 1B
Synonyms: Gm5158
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 382044
HGNC: HGNC:1863
Homologene: 117484
Mmp28
Name: matrix metallopeptidase 28 (epilysin)
Synonyms: epilysin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 118453
Homologene: 41559
Zfp647
Name: zinc finger protein 647
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239546
VEGA: 15
Homologene: 27805
Nrsn1
Name: neurensin 1
Synonyms: Vmp, Neuro-p24, Neurensin-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 22360
Homologene: 7597
Qars
Name: glutaminyl-tRNA synthetase
Synonyms: 1110018N24Rik, 1200016L19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 97541
HGNC: HGNC:9751
Homologene: 133975
Mrgpre
Name: MAS-related GPR, member E
Synonyms: MrgE, C130069N09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 244238
Homologene: 18469
Vmn1r223
Name: vomeronasal 1 receptor 223
Synonyms: Gm11330
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 100036518
Homologene: 110880
Nhsl2
Name: NHS-like 2
Synonyms: 1110062M06Rik, Gm10456
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 100042480
Homologene: 19759
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 81,241,971 bp
  • T to G, chromosome 1 at 136,519,645 bp
  • A to T, chromosome 2 at 28,835,153 bp
  • T to C, chromosome 3 at 87,852,170 bp
  • T to A, chromosome 4 at 43,640,182 bp
  • T to G, chromosome 4 at 59,213,922 bp
  • T to C, chromosome 4 at 94,569,805 bp
  • A to G, chromosome 4 at 127,195,706 bp
  • A to G, chromosome 5 at 45,522,478 bp
  • A to T, chromosome 5 at 129,129,105 bp
  • T to G, chromosome 6 at 17,846,238 bp
  • C to T, chromosome 6 at 35,521,944 bp
  • T to C, chromosome 6 at 93,757,687 bp
  • G to A, chromosome 6 at 124,436,814 bp
  • A to G, chromosome 7 at 24,980,073 bp
  • C to T, chromosome 7 at 143,781,296 bp
  • T to C, chromosome 8 at 93,068,225 bp
  • C to A, chromosome 9 at 73,102,930 bp
  • T to C, chromosome 9 at 106,941,526 bp
  • C to T, chromosome 9 at 108,509,511 bp
  • A to T, chromosome 9 at 110,631,700 bp
  • T to C, chromosome 10 at 18,603,530 bp
  • T to G, chromosome 10 at 68,525,645 bp
  • T to C, chromosome 10 at 77,088,928 bp
  • A to G, chromosome 10 at 81,259,379 bp
  • T to C, chromosome 10 at 116,344,026 bp
  • T to A, chromosome 11 at 4,672,446 bp
  • A to T, chromosome 11 at 19,011,254 bp
  • G to A, chromosome 11 at 73,776,640 bp
  • G to C, chromosome 11 at 74,843,110 bp
  • T to A, chromosome 11 at 83,444,368 bp
  • T to A, chromosome 11 at 101,572,454 bp
  • C to G, chromosome 12 at 84,875,720 bp
  • T to G, chromosome 12 at 89,260,416 bp
  • G to A, chromosome 13 at 23,250,165 bp
  • T to C, chromosome 13 at 25,253,559 bp
  • A to C, chromosome 13 at 63,240,115 bp
  • A to T, chromosome 14 at 119,054,748 bp
  • A to T, chromosome 15 at 76,918,009 bp
  • C to T, chromosome 15 at 97,245,434 bp
  • T to C, chromosome 16 at 45,825,010 bp
  • A to G, chromosome 16 at 87,488,877 bp
  • T to C, chromosome 17 at 37,445,484 bp
  • T to C, chromosome 18 at 5,116,055 bp
  • T to C, chromosome 19 at 58,995,086 bp
  • C to T, chromosome X at 102,077,595 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3076 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040566-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.