Strain Name:
C57BL/6J-MtgxR3087Btlr/Mmmh
Stock Number:
040576-MU
Citation ID:
RRID:MMRRC_040576-MU
Other Names:
R3087 (G1), C57BL/6J-MtgxR3087Btlr
Major Collection:

Strain Information

Spidr
Name: scaffolding protein involved in DNA repair
Synonyms: 2310008H04Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 224008
VEGA: 16
Homologene: 51693
Tlr6
Name: toll-like receptor 6
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 21899
Homologene: 21223
Rps6kb1
Name: ribosomal protein S6 kinase, polypeptide 1
Synonyms: S6K1, p70S6K1, p70/85s6k, 2610318I15Rik, p70s6k
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 72508
Homologene: 81703
Mast4
Name: microtubule associated serine/threonine kinase family member 4
Synonyms: 4930420O11Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 328329
Homologene: 42094
Map4k4
Name: mitogen-activated protein kinase kinase kinase kinase 4
Synonyms: Nik, 9430080K19Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 26921
HGNC: HGNC:6866
Homologene: 7442
Dkk2
Name: dickkopf WNT signaling pathway inhibitor 2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 56811
HGNC: HGNC:2892
Homologene: 8681
Itsn2
Name: intersectin 2
Synonyms: Sh3p18, Ese2, Sh3d1B, Eh domain, SH3 domain regulator of endocytosis 2
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 20403
VEGA: 12
HGNC: HGNC:6184
Homologene: 22627
Flt3
Name: FMS-like tyrosine kinase 3
Synonyms: wmfl, Flk-2, Flk2, Flt-3, CD135
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 14255
HGNC: HGNC:3765
Homologene: 3040
Ttn
Name: titin
Synonyms: shru, L56, mdm, connectin, D330041I19Rik, 2310057K23Rik, 2310074I15Rik, 2310036G12Rik, D830007G01Rik, 1100001C23Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Prdm1
Name: PR domain containing 1, with ZNF domain
Synonyms: Blimp1, Blimp-1, PRDI-BF1, b2b1765Clo
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 12142
HGNC: HGNC:9346
Homologene: 925
Aknad1
Name: AKNA domain containing 1
Synonyms: 4921525H12Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 329738
Homologene: 51892
Cdc16
Name: CDC16 cell division cycle 16
Synonyms: 2700071J12Rik, 2810431D22Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 69957
HGNC: HGNC:1720
Homologene: 2899
Ppic
Name: peptidylprolyl isomerase C
Synonyms: cyclophilin C, CyP-20c
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 19038
VEGA: 18
HGNC: HGNC:9256
Homologene: 727
Cblif
Name: cobalamin binding intrinsic factor
Synonyms: Gif
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 14603
HGNC: HGNC:4268
Homologene: 3773
Stab1
Name: stabilin 1
Synonyms: MS-1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 192187
Homologene: 9035
Pramel25
Name: PRAME like 25
Synonyms: MGC:91194, Gm13023
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 194227
Homologene: 103830
Itgad
Name: integrin, alpha D
Synonyms: Cd11d
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 381924
HGNC: HGNC:6146
Homologene: 56919
Fbll1
Name: fibrillarin-like 1
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237730
Homologene: 28185
Vstm4
Name: V-set and transmembrane domain containing 4
Synonyms: E130203B14Rik
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 320736
Homologene: 18707
Fmo5
Name: flavin containing monooxygenase 5
Synonyms: 5033418D19Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14263
HGNC: HGNC:3773
Homologene: 68185
Pabpc2
Name: poly(A) binding protein, cytoplasmic 2
Synonyms: Pabp2
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 18459
Homologene: 56509
Vmn2r107
Name: vomeronasal 2, receptor 107
Synonyms: V2r6
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 22312
Homologene: 129750
Gm7275
Name: predicted gene 7275
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 639644
VEGA: 16
Arhgef39
Name: Rho guanine nucleotide exchange factor 39
Synonyms: E130306D19Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230098
Homologene: 19225
Cyp2u1
Name: cytochrome P450, family 2, subfamily u, polypeptide 1
Synonyms: 8430436A10Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 71519
Homologene: 77704
Gmeb2
Name: glucocorticoid modulatory element binding protein 2
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 229004
HGNC: HGNC:4371
Homologene: 8228
Mdfic
Name: MyoD family inhibitor domain containing
Synonyms: Kdt1, clone 1.5
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 16543
Homologene: 18404
Ces2e
Name: carboxylesterase 2E
Synonyms: 9030624L02Rik, Ces5
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 234673
HGNC: HGNC:1864
Homologene: 86210
Ifi44l
Name: interferon-induced protein 44 like
Synonyms: NS1178, H-28, H28
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 15061
Homologene: 48468
Vmn1r11
Name: vomeronasal 1 receptor 11
Synonyms: V1rc3
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 113860
Homologene: 128345
Samd13
Name: sterile alpha motif domain containing 13
Synonyms: LOC381481
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 75015
Fam222a
Name: family with sequence similarity 222, member A
Synonyms: BC057022
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 433940
Homologene: 41895
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 40,021,082 bp
  • T to A, chromosome 2 at 76,754,824 bp
  • G to T, chromosome 2 at 181,255,640 bp
  • T to C, chromosome 3 at 97,641,695 bp
  • C to T, chromosome 3 at 108,756,863 bp
  • G to A, chromosome 3 at 131,303,027 bp
  • A to C, chromosome 3 at 132,086,139 bp
  • T to A, chromosome 3 at 146,662,797 bp
  • T to C, chromosome 3 at 151,762,857 bp
  • C to T, chromosome 4 at 43,497,581 bp
  • A to G, chromosome 4 at 143,793,846 bp
  • A to T, chromosome 5 at 64,954,325 bp
  • A to G, chromosome 5 at 114,611,954 bp
  • A to G, chromosome 5 at 147,348,046 bp
  • T to A, chromosome 6 at 15,799,669 bp
  • A to C, chromosome 6 at 57,137,706 bp
  • G to A, chromosome 7 at 128,203,437 bp
  • T to C, chromosome 8 at 13,759,004 bp
  • T to A, chromosome 8 at 104,930,715 bp
  • T to C, chromosome 10 at 44,446,827 bp
  • A to T, chromosome 11 at 35,798,190 bp
  • A to G, chromosome 11 at 86,516,738 bp
  • T to C, chromosome 12 at 4,666,303 bp
  • G to T, chromosome 13 at 102,853,926 bp
  • T to C, chromosome 14 at 31,150,786 bp
  • T to C, chromosome 14 at 32,892,635 bp
  • T to C, chromosome 16 at 15,968,619 bp
  • A to G, chromosome 16 at 48,073,735 bp
  • G to A, chromosome 17 at 20,360,345 bp
  • A to G, chromosome 18 at 39,774,266 bp
  • G to A, chromosome 18 at 53,411,093 bp
  • A to T, chromosome 19 at 11,760,373 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3087 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040576-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.