Strain Name:
C57BL/6J-MtgxR3125Btlr/Mmmh
Stock Number:
040598-MU
Citation ID:
RRID:MMRRC_040598-MU
Other Names:
R3125 (G1), C57BL/6J-MtgxR3125Btlr
Major Collection:

Strain Information

Kit
Name: KIT proto-oncogene receptor tyrosine kinase
Synonyms: Steel Factor Receptor, c-KIT, Dominant white spotting, belly-spot, Tr-kit, SOW3, SCO5, SCO1, Gsfsow3, Gsfsco5, Gsfsco1, CD117
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 16590
HGNC: HGNC:6342
Homologene: 187
Plin2
Name: perilipin 2
Synonyms: Adrp, adipophilin, ADPH, Adfp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 11520
HGNC: HGNC:248
Homologene: 872
Hsd17b12
Name: hydroxysteroid (17-beta) dehydrogenase 12
Synonyms: KIK-I, 2610510O05Rik, keratoadhesin, keratonectin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 56348
Homologene: 95094
Ltbp4
Name: latent transforming growth factor beta binding protein 4
Synonyms: 2310046A13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 108075
HGNC: HGNC:6717
Homologene: 2645
Mat2b
Name: methionine adenosyltransferase II, beta
Synonyms: MATIIbeta, 2410018D16Rik, 1110064C04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 108645
HGNC: HGNC:6905
Homologene: 8323
Exog
Name: endo/exonuclease (5'-3'), endonuclease G-like
Synonyms: ENGL-a, ENDOGL2, ENGL-B, Endogl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 208194
VEGA: 9
HGNC: HGNC:3347
Homologene: 3745
Trappc9
Name: trafficking protein particle complex 9
Synonyms: 4632408O18Rik, 2900005P22Rik, Nibp, TRS130, 1810044A24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 76510
VEGA: 15
Homologene: 81931
Kif15
Name: kinesin family member 15
Synonyms: N-10 kinesin, HKLP2, 3930402I10Rik, 3110023M17Rik, Knsl7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 209737
VEGA: 9
Homologene: 23210
Dyrk1a
Name: dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a
Synonyms: Dyrk, Mnbh, D16Ertd493e, 2310043O08Rik, D16Ertd272e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 13548
HGNC: HGNC:3091
Homologene: 55576
Cep135
Name: centrosomal protein 135
Synonyms: LOC381644, Cep4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 381644
Homologene: 45709
Dlx1
Name: distal-less homeobox 1
Synonyms: DII B, Dlx, Dlx-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 13390
HGNC: HGNC:2914
Homologene: 22558
Icam5
Name: intercellular adhesion molecule 5, telencephalin
Synonyms: CD50, Tlcn, TLN, Icam3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 15898
VEGA: 9
HGNC: HGNC:5348
Homologene: 2447
Dhx32
Name: DEAH (Asp-Glu-Ala-His) box polypeptide 32
Synonyms: Ddx32
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 101437
Homologene: 56798
Htt
Name: huntingtin
Synonyms: huntingtin, HD, htt, IT15, C430023I11Rik, Hdh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 15194
HGNC: HGNC:4851
Homologene: 1593
Pnpla6
Name: patatin-like phospholipase domain containing 6
Synonyms: Swiss-cheese, MSws, Nte
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 50767
Homologene: 21333
Pnpla7
Name: patatin-like phospholipase domain containing 7
Synonyms: E430013P11Rik, NRE
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 241274
Homologene: 62431
Zfp574
Name: zinc finger protein 574
Synonyms: A630056B21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 232976
Homologene: 11238
Dna2
Name: DNA replication helicase/nuclease 2
Synonyms: E130315B21Rik, Dna2l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 327762
HGNC: HGNC:2939
Homologene: 6124
Robo4
Name: roundabout guidance receptor 4
Synonyms: 1200012D01Rik, Magic roundabout
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 74144
Homologene: 10397
Trpm2
Name: transient receptor potential cation channel, subfamily M, member 2
Synonyms: Trrp7, LTRPC2, TRPC7, 9830168K16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 28240
Homologene: 20709
Atp4a
Name: ATPase, H+/K+ exchanging, gastric, alpha polypeptide
Synonyms: H+K+-transporting alpha 1, H+/K+-ATPase alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 11944
HGNC: HGNC:819
Homologene: 68081
Fign
Name: fidgetin
Synonyms: Fgn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 60344
Homologene: 5813
Pthlh
Name: parathyroid hormone-like peptide
Synonyms: Pthrp, parathyroid hormone-like hormone, PTH-like, PTH-related peptide, parathyroid hormone-related peptide, parathyroid hormone-related protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 19227
HGNC: HGNC:9607
Homologene: 2113
Trim9
Name: tripartite motif-containing 9
Synonyms: C030048G07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 94090
VEGA: 12
Homologene: 9045
Trpm6
Name: transient receptor potential cation channel, subfamily M, member 6
Synonyms: CHAK2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 225997
VEGA: 19
Homologene: 9767
Slc6a18
Name: solute carrier family 6 (neurotransmitter transporter), member 18
Synonyms: XT2, D630001K16Rik, Xtrp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 22598
VEGA: 13
Homologene: 40785
Cfap206
Name: cilia and flagella associated protein 206
Synonyms: 1700003M02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 69329
Homologene: 18713
Lonp1
Name: lon peptidase 1, mitochondrial
Synonyms: LON, 1200017E13Rik, Prss15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 74142
VEGA: 17
HGNC: HGNC:9479
Homologene: 3521
D930020B18Rik
Name: RIKEN cDNA D930020B18 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216393
Homologene: 87610
Prr29
Name: proline rich 29
Synonyms: 2310007L24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 75573
Homologene: 130357
Serpina12
Name: serine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 12
Synonyms: vaspin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 68054
Homologene: 23588
Fem1b
Name: fem 1 homolog b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 14155
VEGA: 9
HGNC: HGNC:3649
Homologene: 7714
Fam227b
Name: family with sequence similarity 227, member B
Synonyms: 4930525F21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 75823
Homologene: 27384
Hip1r
Name: huntingtin interacting protein 1 related
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 29816
Homologene: 78348
Ip6k3
Name: inositol hexaphosphate kinase 3
Synonyms: D830007E07Rik, Ihpk3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 271424
Homologene: 65257
Map3k21
Name: mitogen-activated protein kinase kinase kinase 21
Synonyms: BC021891
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234878
Homologene: 32778
Alox5
Name: arachidonate 5-lipoxygenase
Synonyms: 5LO, 5-LOX, 5LX
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 11689
HGNC: HGNC:435
Homologene: 561
Stx6
Name: syntaxin 6
Synonyms: 2310039E05Rik, 2410005I16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 58244
Homologene: 115622
Aoc1l1
Name: amine oxidase copper containing 1-like 1
Synonyms: Doxl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243376
HGNC: HGNC:80
Homologene: 19443
Dcaf8l
Name: DDB1 and CUL4 associated factor 8 like
Synonyms: PC326, PC231, Pex3, Pet2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 18630
Homologene: 137214
Bag4
Name: BCL2-associated athanogene 4
Synonyms: 2410112I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 67384
HGNC: HGNC:940
Homologene: 31270
Togaram1
Name: TOG array regulator of axonemal microtubules 1
Synonyms: A430041B07Rik, Fam179b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 328108
VEGA: 12
Homologene: 15025
Vav3
Name: vav 3 oncogene
Synonyms: A530094I06Rik, Idd18.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 57257
Homologene: 38143
Npb
Name: neuropeptide B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 208990
Homologene: 102151
Or2y3
Name: olfactory receptor family 2 subfamily Y member 3
Synonyms: GA_x6K02T2PSCP-2531299-2530355, MOR256-4, Olfr131
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 258867
Homologene: 105889
Mcpt8
Name: mast cell protease 8
Synonyms: MMCP-8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 17231
VEGA: 14
Dach2
Name: dachshund family transcription factor 2
Synonyms: 9430028N04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 93837
Homologene: 33472
Ifi27l2b
Name: interferon, alpha-inducible protein 27 like 2B
Synonyms: 1810023F06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217845
Homologene: 137395
Or13a22
Name: olfactory receptor family 13 subfamily A member 22
Synonyms: GA_x6K02T2PBJ9-42641642-42642574, MOR253-7, Olfr535
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258956
Homologene: 138320
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 155,158,908 bp
  • A to G, chromosome 2 at 25,042,138 bp
  • T to A, chromosome 2 at 63,978,700 bp
  • T to A, chromosome 2 at 71,532,396 bp
  • C to T, chromosome 2 at 94,033,958 bp
  • T to C, chromosome 2 at 126,124,086 bp
  • G to A, chromosome 3 at 109,628,168 bp
  • T to A, chromosome 4 at 34,716,310 bp
  • G to T, chromosome 4 at 86,657,144 bp
  • T to C, chromosome 5 at 34,804,531 bp
  • G to T, chromosome 5 at 75,647,827 bp
  • C to T, chromosome 5 at 75,647,828 bp
  • T to C, chromosome 5 at 76,621,363 bp
  • A to G, chromosome 5 at 124,000,141 bp
  • A to T, chromosome 6 at 48,975,371 bp
  • A to T, chromosome 6 at 116,427,137 bp
  • A to T, chromosome 6 at 147,263,291 bp
  • G to T, chromosome 7 at 25,081,601 bp
  • C to T, chromosome 7 at 27,327,778 bp
  • G to A, chromosome 7 at 30,720,225 bp
  • T to C, chromosome 7 at 133,725,356 bp
  • T to C, chromosome 7 at 140,492,851 bp
  • G to T, chromosome 8 at 3,534,670 bp
  • C to T, chromosome 8 at 25,769,488 bp
  • A to T, chromosome 8 at 125,941,854 bp
  • A to G, chromosome 9 at 21,036,658 bp
  • CGG to CG, chromosome 9 at 37,411,490 bp
  • T to C, chromosome 9 at 62,796,554 bp
  • A to G, chromosome 9 at 119,448,537 bp
  • A to C, chromosome 9 at 122,987,961 bp
  • G to A, chromosome 10 at 62,949,202 bp
  • T to A, chromosome 10 at 77,911,374 bp
  • T to A, chromosome 10 at 121,678,442 bp
  • A to G, chromosome 11 at 40,684,847 bp
  • A to G, chromosome 11 at 106,374,885 bp
  • G to A, chromosome 11 at 120,608,902 bp
  • G to T, chromosome 12 at 64,966,344 bp
  • C to T, chromosome 12 at 70,248,393 bp
  • T to C, chromosome 12 at 103,451,335 bp
  • G to A, chromosome 12 at 104,037,983 bp
  • A to G, chromosome 13 at 73,677,802 bp
  • A to T, chromosome 14 at 56,083,941 bp
  • G to A, chromosome 15 at 73,025,967 bp
  • G to A, chromosome 16 at 94,668,801 bp
  • T to C, chromosome 17 at 27,157,542 bp
  • G to A, chromosome 17 at 38,082,012 bp
  • A to G, chromosome 17 at 56,626,488 bp
  • C to G, chromosome 19 at 18,854,431 bp
  • A to T, chromosome X at 89,404,721 bp
  • T to C, chromosome X at 113,819,967 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3125 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040598-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.