Strain Name:
C57BL/6J-MtgxR3401Btlr/Mmmh
Stock Number:
040620-MU
Citation ID:
RRID:MMRRC_040620-MU
Other Names:
R3401 (G1), C57BL/6J-MtgxR3401Btlr
Major Collection:

Gene Information

Htr2a
Name: 5-hydroxytryptamine (serotonin) receptor 2A
Synonyms: 5-HT2A receptor, Htr2, Htr-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 15558
VEGA: 14
HGNC: HGNC:5293
Homologene: 68073
Ndrg3
Name: N-myc downstream regulated gene 3
Synonyms: Ndr3, 4833415O14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 29812
Homologene: 40903
Ppp1r37
Name: protein phosphatase 1, regulatory subunit 37
Synonyms: Lrrc68
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 232947
Homologene: 17851
Atrx
Name: ATRX, chromatin remodeler
Synonyms: 4833408C14Rik, XH2, Hp1bp2, DXHXS6677E, Xnp, Rad54, alpha thalassemia/mental retardation syndrome X-linked, HP1-BP38
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 22589
HGNC: HGNC:886
Homologene: 416
Dennd4c
Name: DENN/MADD domain containing 4C
Synonyms: 1700065A05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 329877
Homologene: 23057
Polr3b
Name: polymerase (RNA) III (DNA directed) polypeptide B
Synonyms: RPC2, 2700078H01Rik, A330032P03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 70428
VEGA: 10
Homologene: 6981
Trp53bp2
Name: transformation related protein 53 binding protein 2
Synonyms: ASPP2, 53BP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 209456
Homologene: 3959
Fntb
Name: farnesyltransferase, CAAX box, beta
Synonyms: 2010013E13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 110606
HGNC: HGNC:3785
Homologene: 1535
Ralgapa1
Name: Ral GTPase activating protein, alpha subunit 1
Synonyms: Tulip1, 2310003F20Rik, 4930400K19Rik, Garnl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 56784
VEGA: 12
Homologene: 84805
Cul5
Name: cullin 5
Synonyms: VACM-1, C330021I08Rik, C030032G03Rik, 8430423K24Rik, 4921514I20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 75717
HGNC: HGNC:2556
Homologene: 2597
B3gat1
Name: beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)
Synonyms: GlcAT-P, 0710007K08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 76898
HGNC: HGNC:921
Homologene: 49551
Trim25
Name: tripartite motif-containing 25
Synonyms: estrogen-responsive finger protein, Zfp147
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217069
Homologene: 48325
Pkd2
Name: polycystin 2, transient receptor potential cation channel
Synonyms: PC2, C030034P18Rik, polycystin-2, TRPP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 18764
HGNC: HGNC:9009
Homologene: 20104
Zfp157
Name: zinc finger protein 157
Synonyms: A630094N24Rik, 2610020C11Rik, Roma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 72154
Homologene: 57009
Fli1
Name: Friend leukemia integration 1
Synonyms: Sic1, SIC-1, EWSR2, Fli-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 14247
HGNC: HGNC:3749
Homologene: 55624
Eif1ad
Name: eukaryotic translation initiation factor 1A domain containing
Synonyms: 2010003J03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 69860
VEGA: 19
Homologene: 41860
Naip5
Name: NLR family, apoptosis inhibitory protein 5
Synonyms: Naip-rs3, Birc1e, Lgn1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 17951
HGNC: HGNC:7634
Homologene: 113589
Dnah3
Name: dynein, axonemal, heavy chain 3
Synonyms: Dnahc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 381917
HGNC: HGNC:2949
Homologene: 19674
Stard9
Name: START domain containing 9
Synonyms: 4831403C07Rik, Kif16a, N-3 kinesin, E230025N21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 668880
Homologene: 130712
Tma16
Name: translation machinery associated 16
Synonyms: 1810029B16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 66282
Homologene: 10146
Gucy1a2
Name: guanylate cyclase 1, soluble, alpha 2
Synonyms: 6330407I18Rik, A230060L24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 234889
HGNC: HGNC:4684
Homologene: 47953
Nlrp4d
Name: NLR family, pyrin domain containing 4D
Synonyms: Nalp-beta, Nalp4d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 384752
Sntg2
Name: syntrophin, gamma 2
Synonyms: 2210008K22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 268534
Homologene: 41298
Hip1r
Name: huntingtin interacting protein 1 related
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 29816
Homologene: 78348
Cactin
Name: cactin, spliceosome C complex subunit
Synonyms: 2510012J08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 70312
VEGA: 10
Homologene: 69553
Vmn2r68
Name: vomeronasal 2, receptor 68
Synonyms: EG620697, Vmn2r68-ps
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 620697
Homologene: 115466
Tead2
Name: TEA domain family member 2
Synonyms: TEAD-2, Etdf, TEF-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 21677
Homologene: 19662
Zhx1
Name: zinc fingers and homeoboxes 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 22770
Homologene: 5225
Uprt
Name: uracil phosphoribosyltransferase
Synonyms: LOC331487
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 331487
Homologene: 25175
Faim2
Name: Fas apoptotic inhibitory molecule 2
Synonyms: Tmbim2, NMP25, lifeguard, 2900002L20Rik, Lfg
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 72393
VEGA: 15
Homologene: 8192
Tmem74
Name: transmembrane protein 74
Synonyms: B230382K22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239408
VEGA: 15
Homologene: 17684
Gcsam
Name: germinal center associated, signaling and motility
Synonyms: Gcet2, M17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 14525
Homologene: 49157
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 182,455,872 bp
  • A to G, chromosome 2 at 120,703,689 bp
  • C to T, chromosome 2 at 156,948,288 bp
  • C to T, chromosome 4 at 86,774,543 bp
  • A to G, chromosome 5 at 104,480,327 bp
  • A to G, chromosome 5 at 123,996,983 bp
  • T to A, chromosome 5 at 138,457,011 bp
  • A to T, chromosome 7 at 10,362,854 bp
  • C to T, chromosome 7 at 19,532,787 bp
  • T to A, chromosome 7 at 45,223,673 bp
  • TCC to TC, chromosome 7 at 85,221,550 bp
  • A to G, chromosome 7 at 119,967,656 bp
  • C to T, chromosome 8 at 66,484,171 bp
  • T to A, chromosome 9 at 3,635,154 bp
  • C to A, chromosome 9 at 26,755,853 bp
  • G to A, chromosome 9 at 32,461,274 bp
  • A to T, chromosome 9 at 53,621,212 bp
  • G to A, chromosome 10 at 81,325,875 bp
  • C to T, chromosome 10 at 84,699,491 bp
  • T to C, chromosome 11 at 89,010,881 bp
  • A to G, chromosome 12 at 30,288,172 bp
  • T to C, chromosome 12 at 55,659,137 bp
  • C to A, chromosome 12 at 76,887,888 bp
  • G to A, chromosome 13 at 100,221,903 bp
  • T to C, chromosome 14 at 74,645,059 bp
  • C to T, chromosome 15 at 43,867,021 bp
  • T to C, chromosome 15 at 58,054,349 bp
  • C to T, chromosome 15 at 99,520,348 bp
  • C to T, chromosome 16 at 45,619,821 bp
  • T to C, chromosome 19 at 5,368,248 bp
  • A to G, chromosome X at 104,506,258 bp
  • A to G, chromosome X at 105,888,981 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3401 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040620-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.