Strain Name:
C57BL/6J-MtgxR3414Btlr/Mmmh
Stock Number:
040632-MU
Citation ID:
RRID:MMRRC_040632-MU
Other Names:
R3414 (G1), C57BL/6J-MtgxR3414Btlr
Major Collection:

Gene Information

Inpp5d
Name: inositol polyphosphate-5-phosphatase D
Synonyms: SHIP, SHIP1, SHIP-1, Src homology 2 domain-containing inositol-5-phosphatase, s-SHIP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 16331
HGNC: HGNC:6079
Homologene: 4046
Nos2
Name: nitric oxide synthase 2, inducible
Synonyms: Nos2a, Nos-2, iNOS, NOS-II
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 18126
HGNC: HGNC:7873
Homologene: 55473
Il4i1
Name: interleukin 4 induced 1
Synonyms: H-46, H-4, H4, Fig1, Fig1-ps, H46
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 14204
Homologene: 22567
Sec24a
Name: Sec24 related gene family, member A (S. cerevisiae)
Synonyms: 9430090N21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 77371
Homologene: 70131
Eef2
Name: eukaryotic translation elongation factor 2
Synonyms: Ef-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 13629
VEGA: 10
HGNC: HGNC:3214
Homologene: 134867
Mrps5
Name: mitochondrial ribosomal protein S5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 77721
Homologene: 32726
Ddx18
Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 18
Synonyms: 2310005B10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 66942
HGNC: HGNC:2741
Homologene: 6697
Utp25
Name: UTP25 small subunit processome component
Synonyms: Diexf, AA408296
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 215193
Homologene: 6170
Il7
Name: interleukin 7
Synonyms: hlb368, Il-7, A630026I06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 16196
HGNC: HGNC:6023
Homologene: 680
Lama1
Name: laminin, alpha 1
Synonyms: Lama
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 16772
VEGA: 17
HGNC: HGNC:6481
Homologene: 21146
Nup54
Name: nucleoporin 54
Synonyms: 54kDa, 3110079L04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 269113
Homologene: 41169
Mtus1
Name: mitochondrial tumor suppressor 1
Synonyms: Atip1, MTSG1, B430305I03Rik, MD44
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 102103
Homologene: 100292
Dhx29
Name: DEAH (Asp-Glu-Ala-His) box polypeptide 29
Synonyms: E130202M19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 218629
VEGA: 13
Homologene: 10387
AW554918
Name: expressed sequence AW554918
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 225289
Homologene: 52652
Zfhx4
Name: zinc finger homeodomain 4
Synonyms: Zfh-4, C130041O22Rik, Zfh4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 80892
Homologene: 23477
Ttn
Name: titin
Synonyms: shru, connectin, mdm, 2310057K23Rik, D330041I19Rik, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, L56, 2310074I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22138
Homologene: 130650
Rusc2
Name: RUN and SH3 domain containing 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 100213
Homologene: 18967
Unc13b
Name: unc-13 homolog B
Synonyms: Munc13-2, Unc13h2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 22249
Homologene: 31376
Naip2
Name: NLR family, apoptosis inhibitory protein 2
Synonyms: Naip2, Birc1b, Naip-rs6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 17948
HGNC: HGNC:7634
Homologene: 136092
Trim69
Name: tripartite motif-containing 69
Synonyms: 4921519C19Rik, Trif, Rnf36
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 70928
Homologene: 18827
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 380698
Homologene: 70869
Proc
Name: protein C
Synonyms: PC, inactivator of coagulation factors Va, VIII
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 19123
VEGA: 18
HGNC: HGNC:9451
Homologene: 37288
Tusc1
Name: tumor suppressor candidate 1
Synonyms: TSG-9, 2200001D17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 69136
Homologene: 84597
Psg21
Name: pregnancy-specific glycoprotein 21
Synonyms: 1600025N01Rik, 1600026N13Rik, 1600019C01Rik, cea8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 72242
HGNC: null
Homologene: 110989
Ppip5k1
Name: diphosphoinositol pentakisphosphate kinase 1
Synonyms: B430315C20Rik, Hisppd2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 327655
Homologene: 49395
Nsd3
Name: nuclear receptor binding SET domain protein 3
Synonyms: WHISTLE, Whsc1l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 234135
Homologene: 56960
Cttnbp2
Name: cortactin binding protein 2
Synonyms: ORF4, 3010022N24Rik, Cortbp2, 9130022E09Rik, 4732477G22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 30785
Homologene: 14125
3425401B19Rik
Name: RIKEN cDNA 3425401B19 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 100504518
VEGA: 14
Homologene: 54908
Spata13
Name: spermatogenesis associated 13
Synonyms: ESTM11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 219140
Homologene: 19482
Ifi202b
Name: interferon activated gene 202B
Synonyms: p202
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 26388
HGNC: null
Homologene: null
Fcna
Name: ficolin A
Synonyms: Fcn1, ficolin A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 14133
HGNC: null
Homologene: 117949
Atp8b4
Name: ATPase, class I, type 8B, member 4
Synonyms: Im
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 241633
Homologene: 133162
Ergic2
Name: ERGIC and golgi 2
Synonyms: 4930572C01Rik, 1200009B18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 67456
Homologene: 6574
Pla2g4f
Name: phospholipase A2, group IVF
Synonyms: Pla2zeta, 4732472I07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 271844
Homologene: 77933
Hace1
Name: HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1
Synonyms: 1700042J16Rik, A730034A22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 209462
Homologene: 10807
Slc1a7
Name: solute carrier family 1 (glutamate transporter), member 7
Synonyms: EAAT5, A930031E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 242607
Homologene: 21327
Olfr808
Name: olfactory receptor 808
Synonyms: MOR112-2, GA_x6K02T2PULF-11446184-11447122
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 258930
Homologene: 17438
Top1mt
Name: DNA topoisomerase 1, mitochondrial
Synonyms: 2900052H09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 72960
Homologene: 43082
Olfr156
Name: olfactory receptor 156
Synonyms: OR37B, GA_x6K02T2N78B-16125144-16126100, mOR37b, Olfr37b, MOR262-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 29846
Homologene: 133652
Spag8
Name: sperm associated antigen 8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 433700
Homologene: 8250
Vmn1r2
Name: vomeronasal 1 receptor 2
Synonyms: Gm11776
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 100312470
HGNC: null
Homologene: 128342
Klrc1
Name: killer cell lectin-like receptor subfamily C, member 1
Synonyms: CD159a, NKG2B, NKG2A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 16641
HGNC: HGNC:6374
Homologene: 86848
Olfr710
Name: olfactory receptor 710
Synonyms: GA_x6K02T2PBJ9-9325348-9324416, MOR260-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 258594
HGNC: null
Homologene: 27218
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 87,668,057 bp
  • T to C, chromosome 1 at 121,562,149 bp
  • A to G, chromosome 1 at 173,963,913 bp
  • A to C, chromosome 1 at 193,128,502 bp
  • G to C, chromosome 2 at 25,627,493 bp
  • A to T, chromosome 2 at 76,811,243 bp
  • T to C, chromosome 2 at 120,303,106 bp
  • A to T, chromosome 2 at 121,327,661 bp
  • T to C, chromosome 2 at 122,178,644 bp
  • C to A, chromosome 2 at 126,375,757 bp
  • A to G, chromosome 2 at 127,596,912 bp
  • A to G, chromosome 3 at 5,403,823 bp
  • G to T, chromosome 3 at 7,576,033 bp
  • T to A, chromosome 4 at 3,172,696 bp
  • T to A, chromosome 4 at 43,234,658 bp
  • T to G, chromosome 4 at 43,415,935 bp
  • T to A, chromosome 4 at 43,651,606 bp
  • C to T, chromosome 4 at 43,821,258 bp
  • C to A, chromosome 4 at 93,334,936 bp
  • A to G, chromosome 4 at 108,010,994 bp
  • A to G, chromosome 5 at 92,431,027 bp
  • A to G, chromosome 6 at 18,389,205 bp
  • A to G, chromosome 6 at 129,677,763 bp
  • A to G, chromosome 6 at 148,206,681 bp
  • A to T, chromosome 7 at 18,652,380 bp
  • T to C, chromosome 7 at 44,836,658 bp
  • G to T, chromosome 7 at 106,944,176 bp
  • T to C, chromosome 8 at 25,700,019 bp
  • T to C, chromosome 8 at 41,048,063 bp
  • T to A, chromosome 10 at 45,648,675 bp
  • G to T, chromosome 10 at 81,177,858 bp
  • A to G, chromosome 10 at 129,768,432 bp
  • T to A, chromosome 11 at 51,729,458 bp
  • C to G, chromosome 11 at 59,067,930 bp
  • A to T, chromosome 11 at 78,957,588 bp
  • G to A, chromosome 13 at 100,189,263 bp
  • A to G, chromosome 13 at 112,947,273 bp
  • T to A, chromosome 14 at 32,661,602 bp
  • A to G, chromosome 14 at 60,706,723 bp
  • T to C, chromosome 15 at 75,657,176 bp
  • T to C, chromosome 17 at 67,737,603 bp
  • C to A, chromosome 18 at 25,400,072 bp
  • T to A, chromosome 18 at 32,123,685 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3414 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040632-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.