Strain Name:
C57BL/6J-MtgxR3435Btlr/Mmmh
Stock Number:
040653-MU
Citation ID:
RRID:MMRRC_040653-MU
Other Names:
R3435 (G1), C57BL/6J-MtgxR3435Btlr
Major Collection:

Gene Information

Notch3
Name: notch 3
Synonyms: N3, hpbk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 18131
HGNC: HGNC:7883
Homologene: 376
Sema6d
Name: sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D
Synonyms: Sema6D-2, Sema6D-5, Sema6D-1, Sema6D-6, 1110067B02Rik, Sema6D-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 214968
Homologene: 16195
Strn4
Name: striatin, calmodulin binding protein 4
Synonyms: ZIN, zinedin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 97387
Homologene: 8378
Mast2
Name: microtubule associated serine/threonine kinase 2
Synonyms: MAST205, Mtssk
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 17776
Homologene: 7428
Mast4
Name: microtubule associated serine/threonine kinase family member 4
Synonyms: 4930420O11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 328329
Homologene: 42094
Mdn1
Name: midasin AAA ATPase 1
Synonyms: LOC213784, 4833432B22Rik, D4Abb1e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100019
Homologene: 39689
Ift74
Name: intraflagellar transport 74
Synonyms: Ccdc2, 1700029H06Rik, Cmg1, b2b796Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 67694
Homologene: 11831
Iqgap3
Name: IQ motif containing GTPase activating protein 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 404710
Homologene: 26400
Neurl1a
Name: neuralized E3 ubiquitin protein ligase 1A
Synonyms: Neurl, Nlz, Neur1, Neu1, Rnf67, 2410129E16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 18011
VEGA: 19
HGNC: HGNC:7761
Homologene: 32503
Atp7a
Name: ATPase, Cu++ transporting, alpha polypeptide
Synonyms: Menkes protein, MNK, br
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 11977
HGNC: HGNC:869
Homologene: 35
Dnah1
Name: dynein, axonemal, heavy chain 1
Synonyms: MDHC7, Dnahc1, B230373P09Rik, E030034C22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 110084
VEGA: 14
HGNC: HGNC:2940
Homologene: 67131
P2rx4
Name: purinergic receptor P2X, ligand-gated ion channel 4
Synonyms: D5Ertd444e, P2X4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 18438
HGNC: HGNC:8535
Homologene: 1923
Ttn
Name: titin
Synonyms: D830007G01Rik, 2310057K23Rik, L56, shru, connectin, 2310074I15Rik, mdm, 2310036G12Rik, 1100001C23Rik, D330041I19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Pld1
Name: phospholipase D1
Synonyms: Pld1a, Pld1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 18805
HGNC: HGNC:9067
Homologene: 116234
AC159748.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Abca9
Name: ATP-binding cassette, sub-family A (ABC1), member 9
Synonyms: D630040K07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217262
HGNC: HGNC:39
Homologene: 33332
Mup6
Name: major urinary protein 6
Synonyms: Gm12544
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 620807
Homologene: 74304
4933409G03Rik
Name: RIKEN cDNA 4933409G03 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227998
Osbpl3
Name: oxysterol binding protein-like 3
Synonyms: OSBP3, ORP3, 1200014M06Rik, 6720421I08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 71720
Homologene: 49422
Tmx3
Name: thioredoxin-related transmembrane protein 3
Synonyms: Txndc10, A730024F05Rik, 6430411B10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 67988
VEGA: 18
Homologene: 10381
Prob1
Name: proline rich basic protein 1
Synonyms: Gm1614, LOC381148
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 381148
Homologene: 83773
Nod2
Name: nucleotide-binding oligomerization domain containing 2
Synonyms: F830032C23Rik, Card15, Nlrc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 257632
HGNC: HGNC:5331
Homologene: 11156
Larp1b
Name: La ribonucleoprotein domain family, member 1B
Synonyms: 4933421B21Rik, 1700108L22Rik, Larp2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 214048
Homologene: 103869
Vmn1r209
Name: vomeronasal 1 receptor 209
Synonyms: Gm11315
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 432736
Homologene: 110880
Tmem117
Name: transmembrane protein 117
Synonyms: B930062P21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 320709
VEGA: 15
Homologene: 12992
Stard13
Name: StAR-related lipid transfer (START) domain containing 13
Synonyms: DLC2, GT650
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 243362
Homologene: 64844
Olfr3
Name: olfactory receptor 3
Synonyms: GA_x6K02T2NLDC-33507606-33506665, Y71, MOR136-14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18328
HGNC: HGNC:8208
Homologene: 66579
Pbxip1
Name: pre B cell leukemia transcription factor interacting protein 1
Synonyms: 4732463H20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229534
Homologene: 10740
Fam47e
Name: family with sequence similarity 47, member E
Synonyms: LOC384198, Gm1381
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 384198
Homologene: 129958
Ppp2r2b
Name: protein phosphatase 2, regulatory subunit B, beta
Synonyms: PR55-BETA, E130009M08Rik, SCA12, PP2A-PR55B, 6330404L05Rik, 2900026H06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 72930
HGNC: HGNC:9305
Homologene: 55833
AC132379.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Mafk
Name: v-maf musculoaponeurotic fibrosarcoma oncogene family, protein K (avian)
Synonyms: Nfe2u, p18 NF-E2, NF-E2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 17135
HGNC: HGNC:6782
Homologene: 1770
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 116,821,623 bp
  • T to A, chromosome 2 at 36,812,678 bp
  • A to C, chromosome 2 at 68,601,845 bp
  • T to C, chromosome 2 at 76,878,718 bp
  • T to C, chromosome 2 at 124,660,274 bp
  • A to T, chromosome 3 at 28,124,623 bp
  • T to C, chromosome 3 at 41,034,098 bp
  • A to G, chromosome 3 at 88,094,604 bp
  • T to C, chromosome 3 at 89,447,236 bp
  • T to C, chromosome 4 at 32,733,726 bp
  • G to T, chromosome 4 at 60,004,116 bp
  • A to G, chromosome 4 at 94,621,852 bp
  • A to T, chromosome 4 at 116,308,095 bp
  • T to A, chromosome 5 at 92,585,362 bp
  • T to A, chromosome 5 at 122,725,070 bp
  • C to T, chromosome 5 at 139,800,307 bp
  • A to T, chromosome 5 at 151,042,179 bp
  • A to T, chromosome 6 at 50,348,070 bp
  • T to C, chromosome 7 at 16,837,633 bp
  • A to G, chromosome 8 at 88,664,009 bp
  • T to C, chromosome 10 at 5,348,565 bp
  • T to C, chromosome 11 at 110,154,430 bp
  • A to T, chromosome 13 at 22,806,097 bp
  • A to G, chromosome 13 at 102,787,379 bp
  • T to C, chromosome 14 at 31,316,674 bp
  • T to C, chromosome 15 at 95,094,692 bp
  • A to T, chromosome 17 at 32,158,618 bp
  • T to C, chromosome 18 at 35,654,241 bp
  • T to A, chromosome 18 at 42,741,109 bp
  • T to A, chromosome 18 at 90,527,904 bp
  • T to C, chromosome 19 at 47,257,525 bp
  • G to A, chromosome X at 106,094,857 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3435 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040653-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.