Strain Name:
C57BL/6J-MtgxR3439Btlr/Mmmh
Stock Number:
040657-MU
Citation ID:
RRID:MMRRC_040657-MU
Other Names:
R3439 (G1), C57BL/6J-MtgxR3439Btlr
Major Collection:

Gene Information

Prrc2b
Name: proline-rich coiled-coil 2B
Synonyms: 5830434P21Rik, Bat2l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 227723
Homologene: 106649
Vpreb3
Name: pre-B lymphocyte gene 3
Synonyms: 8HS-20, Vpreb-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 22364
Homologene: 7598
Tfr2
Name: transferrin receptor 2
Synonyms: Tfr2, Trfr2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 50765
Homologene: 2428
Psmd3
Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 3
Synonyms: Tstap91a, AntP91a, Psd3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 22123
HGNC: HGNC:9560
Homologene: 2102
Srbd1
Name: S1 RNA binding domain 1
Synonyms: D530025C17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 78586
VEGA: 17
Homologene: 9994
Tet2
Name: tet methylcytosine dioxygenase 2
Synonyms: Ayu17-449, E130014J05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 214133
Homologene: 49498
Palm
Name: paralemmin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 18483
VEGA: 10
HGNC: HGNC:8594
Homologene: 1937
Helq
Name: helicase, POLQ-like
Synonyms: D430018E21Rik, Hel308
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 191578
Homologene: 14667
Med13
Name: mediator complex subunit 13
Synonyms: Trap240, 1110067M05Rik, Thrap1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 327987
Homologene: 21067
Lingo1
Name: leucine rich repeat and Ig domain containing 1
Synonyms: LERN1, LINGO-1, 4930471K13Rik, Lrrn6a, UNQ201
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 235402
Homologene: 41891
Dnhd1
Name: dynein heavy chain domain 1
Synonyms: 8030491N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 77505
Homologene: 131117
Gnb1l
Name: guanine nucleotide binding protein (G protein), beta polypeptide 1-like
Synonyms: Wdvcf, ESTM55, Wdr14, Me49f07
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 13972
HGNC: HGNC:4397
Homologene: 41515
Pitpnm1
Name: phosphatidylinositol transfer protein, membrane-associated 1
Synonyms: RdgB, DRES9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 18739
HGNC: HGNC:9003
Homologene: 3608
Dnah10
Name: dynein, axonemal, heavy chain 10
Synonyms: Dnahc10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 56087
HGNC: HGNC:2941
Homologene: 25816
Lrrc37a
Name: leucine rich repeat containing 37A
Synonyms: LOC237954
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 237954
Homologene: 138824
5430419D17Rik
Name: RIKEN cDNA 5430419D17 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 71395
HGNC: null
Homologene: 138430
Kng2
Name: kininogen 2
Synonyms: Kininogen-II
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 385643
HGNC: HGNC:6383
Homologene: 88343
Acss3
Name: acyl-CoA synthetase short-chain family member 3
Synonyms: LOC380660, 8430416H19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 380660
Homologene: 11587
Rnpepl1
Name: arginyl aminopeptidase (aminopeptidase B)-like 1
Synonyms: 1110014H17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 108657
Homologene: 36367
Mthfsd
Name: methenyltetrahydrofolate synthetase domain containing
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 234814
Homologene: 11245
Topaz1
Name: testis and ovary specific PAZ domain containing 1
Synonyms: Gm9524
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 671232
VEGA: 9
Homologene: 19835
Vwde
Name: von Willebrand factor D and EGF domains
Synonyms: LOC232585
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 232585
Homologene: 35456
Nwd2
Name: NACHT and WD repeat domain containing 2
Synonyms: B830017A01Rik, 3110047P20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 319807
Homologene: 14974
Agxt2
Name: alanine-glyoxylate aminotransferase 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 268782
Homologene: 12887
Slc41a2
Name: solute carrier family 41, member 2
Synonyms: A230035L05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 338365
Homologene: 9870
Tchh
Name: trichohyalin
Synonyms: AHF, Thh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 99681
Homologene: 136273
Glb1l
Name: galactosidase, beta 1-like
Synonyms: 4833408P15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 74577
Homologene: 75208
Serpinb7
Name: serine (or cysteine) peptidase inhibitor, clade B, member 7
Synonyms: ovalbumin, 4631416M05Rik, megsin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 116872
Homologene: 68363
Olfr1311
Name: olfactory receptor 1311
Synonyms: GA_x6K02T2Q125-73069292-73068354, MOR245-21
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258271
HGNC: null
Homologene: 133656
Try4
Name: trypsin 4
Synonyms: Td, 0910001B19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 22074
HGNC: HGNC:9486
Homologene: 106639
Plod3
Name: procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
Synonyms: lysyl hydroxylase 3, LH3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 26433
HGNC: HGNC:9083
Homologene: 843
Nom1
Name: nucleolar protein with MIF4G domain 1
Synonyms: LOC381627, D5Kng1, Gm1040
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 433864
Homologene: 39776
Olfr235
Name: olfactory receptor 235
Synonyms: GA_x6K02T057QT-4025-4642, GA_x6K02T03CT6-1-477, Olfr245, GA_x6K02T2LL2P-1028-792, MOR214-3, Olfr232, MOR214-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 258681
HGNC: null
Homologene: null
Gm12666
Name: predicted gene 12666
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 68280
HGNC: null
Homologene: null
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 75,202,620 bp
  • A to T, chromosome 1 at 92,916,940 bp
  • A to T, chromosome 1 at 107,428,351 bp
  • T to C, chromosome 2 at 32,206,347 bp
  • T to C, chromosome 2 at 112,021,447 bp
  • A to G, chromosome 3 at 93,447,393 bp
  • G to T, chromosome 3 at 133,466,831 bp
  • A to C, chromosome 4 at 92,191,682 bp
  • T to C, chromosome 5 at 29,435,617 bp
  • G to A, chromosome 5 at 63,804,552 bp
  • T to C, chromosome 5 at 100,798,304 bp
  • T to C, chromosome 5 at 124,796,258 bp
  • G to C, chromosome 5 at 136,988,146 bp
  • G to T, chromosome 5 at 137,574,651 bp
  • T to A, chromosome 6 at 13,208,375 bp
  • T to C, chromosome 6 at 41,305,087 bp
  • A to G, chromosome 7 at 105,694,785 bp
  • T to C, chromosome 7 at 131,188,779 bp
  • A to G, chromosome 8 at 121,099,121 bp
  • T to C, chromosome 9 at 56,620,733 bp
  • A to T, chromosome 9 at 122,750,687 bp
  • C to T, chromosome 10 at 75,943,222 bp
  • C to T, chromosome 10 at 79,816,784 bp
  • G to T, chromosome 10 at 83,301,321 bp
  • A to T, chromosome 10 at 107,004,826 bp
  • T to C, chromosome 11 at 86,285,297 bp
  • TCCCCCCCCCCCCCC to TCCCCCCCCCCCCCCCCCCC,TCCCCCCCCCCC,TCCCCCCCCCCCC, chromosome 11 at 98,693,788 bp
  • G to A, chromosome 11 at 103,497,864 bp
  • C to A, chromosome 15 at 10,381,425 bp
  • A to G, chromosome 16 at 18,552,367 bp
  • T to C, chromosome 16 at 23,012,071 bp
  • C to T, chromosome 17 at 86,057,759 bp
  • A to G, chromosome 19 at 4,112,752 bp
  • A to T, chromosome 19 at 12,268,395 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3439 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040657-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.