Strain Name:
C57BL/6J-MtgxR3547Btlr/Mmmh
Stock Number:
040666-MU
Citation ID:
RRID:MMRRC_040666-MU
Other Names:
R3547 (G1), C57BL/6J-MtgxR3547Btlr
Major Collection:

Gene Information

Pi4k2a
Name: phosphatidylinositol 4-kinase type 2 alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 84095
VEGA: 19
Homologene: 101681
Utp20
Name: UTP20 small subunit processome component
Synonyms: DRIM, mDRIM, 3830408P06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 70683
VEGA: 10
Homologene: 38373
Arid5b
Name: AT rich interactive domain 5B (MRF1-like)
Synonyms: 5430435G07Rik, Desrt, Mrf2beta, Mrf2alpha, Mrf2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 71371
VEGA: 10
Homologene: 45872
Lcmt1
Name: leucine carboxyl methyltransferase 1
Synonyms: Lcmt, LCMT-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 30949
Homologene: 41123
Usp9x
Name: ubiquitin specific peptidase 9, X chromosome
Synonyms: Dffrx, Fafl, 5730589N07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 22284
Homologene: 3418
Eprs
Name: glutamyl-prolyl-tRNA synthetase
Synonyms: 2410081F06Rik, 3010002K18Rik, Qprs
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 107508
HGNC: HGNC:3418
Homologene: 5870
Gstcd
Name: glutathione S-transferase, C-terminal domain containing
Synonyms: 4933434L15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 67553
Homologene: 11693
Anapc1
Name: anaphase promoting complex subunit 1
Synonyms: tsg24, Apc1, 2610021O03Rik, Mcpr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17222
Homologene: 7414
Ube4b
Name: ubiquitination factor E4B
Synonyms: UFD2, 4933406G05Rik, 4930551I19Rik, UFD2a, Ufd2p, D4Bwg0973e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 63958
Homologene: 107623
Clcc1
Name: chloride channel CLIC-like 1
Synonyms: Mclc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229725
Homologene: 9032
Prkcq
Name: protein kinase C, theta
Synonyms: PKC-0, Pkcq, A130035A12Rik, PKC-theta, PKC theta, PKCtheta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18761
HGNC: HGNC:9410
Homologene: 21263
Dmbt1
Name: deleted in malignant brain tumors 1
Synonyms: CRP-[b], CRP-[a], ebnerin, hensin, vomeroglandin, Crpd, MUCLIN, gp300
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 12945
HGNC: HGNC:2926
Homologene: 68990
Tbc1d1
Name: TBC1 domain family, member 1
Synonyms: 1110062G02Rik, Nob1, Nobq1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 57915
Homologene: 56856
Vps13d
Name: vacuolar protein sorting 13D
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230895
Homologene: 15583
Uty
Name: ubiquitously transcribed tetratricopeptide repeat containing, Y-linked
Synonyms: Hydb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: Y
NCBI: 22290
Rnf167
Name: ring finger protein 167
Synonyms: 0610010G05Rik, 5730408C10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 70510
Homologene: 41046
Prune2
Name: prune homolog 2
Synonyms: 6330414G02Rik, A330102H22Rik, A230083H22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 353211
VEGA: 19
Homologene: 18939
Nbeal1
Name: neurobeachin like 1
Synonyms: A530050O19Rik, ALS2CR17, A530083I02Rik, 2310076G13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 269198
Homologene: 16453
Csmd1
Name: CUB and Sushi multiple domains 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 94109
Homologene: 69536
Reln
Name: reelin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 19699
HGNC: HGNC:9957
Homologene: 3699
Pitx3
Name: paired-like homeodomain transcription factor 3
Synonyms: Ptx3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 18742
HGNC: HGNC:9006
Homologene: 3689
Hydin
Name: HYDIN, axonemal central pair apparatus protein
Synonyms: hy-3, hy3, 1700034M11Rik, 4930545D19Rik, hyrh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 244653
Homologene: 52118
Fbn1
Name: fibrillin 1
Synonyms: Fib-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14118
HGNC: HGNC:3603
Homologene: 30958
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22138
Homologene: 130650
Zfp831
Name: zinc finger protein 831
Synonyms: ENSMUSG00000050600, OTTMUSG00000017459
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 100043757
Homologene: 19013
Col12a1
Name: collagen, type XII, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12816
HGNC: HGNC:2188
Homologene: 3217
Antxr2
Name: anthrax toxin receptor 2
Synonyms: cI-35, CMG-2, CMG2, 2310046B19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 71914
Homologene: 43236
Lrp1b
Name: low density lipoprotein-related protein 1B
Synonyms: 9630004P12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 94217
HGNC: HGNC:6693
Homologene: 56810
Golga7b
Name: golgi autoantigen, golgin subfamily a, 7B
Synonyms: 4933417O08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 71146
Homologene: 28514
Clec4a3
Name: C-type lectin domain family 4, member a3
Synonyms: 3110037K17Rik, mDcir3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 73149
Homologene: 9413
Arl9
Name: ADP-ribosylation factor-like 9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 384185
Homologene: 66207
Ugt8a
Name: UDP galactosyltransferase 8A
Synonyms: Cgt, mCerGT, Ugt8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 22239
Homologene: 20715
Slc16a7
Name: solute carrier family 16 (monocarboxylic acid transporters), member 7
Synonyms: MCT2, 4921534N07Rik, D630004K10Rik, 9030411M13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 20503
Homologene: 20990
Fsip1
Name: fibrous sheath-interacting protein 1
Synonyms: 4933432K11Rik, 1700012M13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 71313
Homologene: 12390
Slc25a45
Name: solute carrier family 25, member 45
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 107375
Homologene: 76679
Igsf10
Name: immunoglobulin superfamily, member 10
Synonyms: 6530405F15Rik, CMF608, Adlican2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 242050
Homologene: 18712
Rufy4
Name: RUN and FYVE domain containing 4
Synonyms: F930048N03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 435626
Homologene: 52359
Slc6a20a
Name: solute carrier family 6 (neurotransmitter transporter), member 20A
Synonyms: A730081N20Rik, Xtrp3s1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 102680
Homologene: 10625
Prickle2
Name: prickle planar cell polarity protein 2
Synonyms: 6720451F06Rik, 6230400G14Rik, mpk2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243548
Homologene: 17889
Kcnh7
Name: potassium voltage-gated channel, subfamily H (eag-related), member 7
Synonyms: Kv11.3, erg3, 9330137I11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 170738
Homologene: 13249
Grid2
Name: glutamate receptor, ionotropic, delta 2
Synonyms: GluRdelta2, tpr, B230104L07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14804
HGNC: HGNC:4576
Homologene: 74399
Ncf1
Name: neutrophil cytosolic factor 1
Synonyms: p47phox, NADPH oxidase subunit (47kDa), Ncf-1, NOXO2, p47phox
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 17969
HGNC: HGNC:7660
Homologene: 30964
Col20a1
Name: collagen, type XX, alpha 1
Synonyms: 1700051I12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 73368
Homologene: 75138
Fap
Name: fibroblast activation protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14089
HGNC: HGNC:3590
Homologene: 48282
Clip1
Name: CAP-GLY domain containing linker protein 1
Synonyms: cytoplasmic linker protein 50, Clip50, CLIP-170, 1110007I12Rik, 4631429H07Rik, Rsn, Clip 170, restin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 56430
Homologene: 74455
Smarca5
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5
Synonyms: Snf2h, D330027N15Rik, 4933427E24Rik, D030040M08Rik, MommeD4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 93762
Homologene: 55764
Map3k3
Name: mitogen-activated protein kinase kinase kinase 3
Synonyms: MAPKKK3, Mekk3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 26406
HGNC: HGNC:6855
Homologene: 69110
Atxn1l
Name: ataxin 1-like
Synonyms: C330011L24Rik, ENSMUSG00000069895, Boat, D8Ertd587e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 52335
Homologene: 19471
Vmn1r7
Name: vomeronasal 1 receptor 7
Synonyms: Gm5568, V1rc31
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 434016
Homologene: 83427
Nat1
Name: N-acetyl transferase 1
Synonyms: Nat-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 17960
Homologene: 115468
Fhit
Name: fragile histidine triad gene
Synonyms: Fra14A2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 14198
HGNC: HGNC:3701
Homologene: 21661
Gpr149
Name: G protein-coupled receptor 149
Synonyms: PGR10, 9630018L10Rik, Ieda, R35
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229357
Homologene: 16359
Nlrp6
Name: NLR family, pyrin domain containing 6
Synonyms: Nalp6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 101613
Homologene: 15881
Celf3
Name: CUGBP, Elav-like family member 3
Synonyms: BRUNOL1, ERDA4, CAGH4, 4930415M08Rik, Tnrc4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 78784
Homologene: 48510
Thnsl1
Name: threonine synthase-like 1 (bacterial)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 208967
Homologene: 32609
Olfr549
Name: olfactory receptor 549
Synonyms: GA_x6K02T2PBJ9-5274337-5275287, MOR31-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 259105
Homologene: 86245
Uprt
Name: uracil phosphoribosyltransferase
Synonyms: LOC331487
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 331487
Homologene: 25175
Zfp319
Name: zinc finger protein 319
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 79233
Homologene: 11548
Sfxn2
Name: sideroflexin 2
Synonyms: F630107H02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 94279
VEGA: 19
Homologene: 14227
Lag3
Name: lymphocyte-activation gene 3
Synonyms: CD223, Ly66, LAG-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16768
HGNC: HGNC:6476
Homologene: 1719
Slc22a17
Name: solute carrier family 22 (organic cation transporter), member 17
Synonyms: 1700094C23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 59049
VEGA: 14
Homologene: 10684
Cx3cl1
Name: chemokine (C-X3-C motif) ligand 1
Synonyms: fractalkine, CX3C, neurotactin, D8Bwg0439e, Scyd1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 20312
Homologene: 2251
Alg5
Name: asparagine-linked glycosylation 5 (dolichyl-phosphate beta-glucosyltransferase)
Synonyms: 1500026A19Rik, 2600005J22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 66248
Homologene: 7060
Mcf2
Name: mcf.2 transforming sequence
Synonyms: Dbl, B230117G22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 109904
HGNC: HGNC:6940
Homologene: 3917
Ly6m
Name: lymphocyte antigen 6 complex, locus M
Synonyms: 2010109I03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 67038
Ppp1r27
Name: protein phosphatase 1, regulatory subunit 27
Synonyms: toonin, 1110033I14Rik, Dysfip1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 68701
Homologene: 45933
Ac155333.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Gdi1
Name: guanosine diphosphate (GDP) dissociation inhibitor 1
Synonyms: GDIA, Rab GDIalpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 14567
HGNC: HGNC:4226
Homologene: 37487
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 60,278,780 bp
  • T to C, chromosome 1 at 74,147,663 bp
  • G to A, chromosome 1 at 185,379,742 bp
  • A to G, chromosome 2 at 11,283,816 bp
  • T to G, chromosome 2 at 21,212,627 bp
  • A to T, chromosome 2 at 40,600,288 bp
  • A to C, chromosome 2 at 62,519,011 bp
  • A to G, chromosome 2 at 62,764,780 bp
  • T to A, chromosome 2 at 76,745,106 bp
  • AAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGA to AAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGA, chromosome 2 at 118,232,853 bp
  • C to T, chromosome 2 at 125,317,549 bp
  • T to C, chromosome 2 at 128,642,682 bp
  • A to G, chromosome 2 at 174,657,683 bp
  • G to T, chromosome 2 at 180,994,911 bp
  • A to G, chromosome 3 at 54,749,315 bp
  • G to A, chromosome 3 at 59,330,541 bp
  • C to A, chromosome 3 at 59,336,514 bp
  • A to T, chromosome 3 at 62,595,128 bp
  • T to G, chromosome 3 at 94,488,538 bp
  • T to A, chromosome 3 at 108,668,113 bp
  • A to T, chromosome 3 at 125,867,382 bp
  • G to T, chromosome 3 at 133,084,838 bp
  • T to G, chromosome 4 at 145,074,975 bp
  • T to C, chromosome 4 at 149,335,116 bp
  • A to G, chromosome 5 at 22,227,600 bp
  • G to A, chromosome 5 at 64,286,007 bp
  • A to G, chromosome 5 at 77,010,479 bp
  • A to T, chromosome 5 at 97,977,657 bp
  • A to G, chromosome 5 at 123,631,078 bp
  • T to A, chromosome 5 at 134,226,609 bp
  • A to G, chromosome 6 at 57,024,849 bp
  • T to C, chromosome 6 at 64,320,021 bp
  • A to G, chromosome 6 at 69,776,256 bp
  • T to C, chromosome 6 at 92,411,137 bp
  • A to G, chromosome 6 at 122,964,280 bp
  • GAAA to GAA, chromosome 6 at 124,904,611 bp
  • A to G, chromosome 7 at 102,554,470 bp
  • A to G, chromosome 7 at 123,400,479 bp
  • T to C, chromosome 7 at 131,088,870 bp
  • T to G, chromosome 7 at 140,926,769 bp
  • A to G, chromosome 8 at 15,915,391 bp
  • A to G, chromosome 8 at 67,491,032 bp
  • C to A, chromosome 8 at 80,717,652 bp
  • A to C, chromosome 8 at 94,778,124 bp
  • A to G, chromosome 8 at 95,328,817 bp
  • A to G, chromosome 8 at 109,732,349 bp
  • G to A, chromosome 8 at 110,582,067 bp
  • T to G, chromosome 9 at 79,633,416 bp
  • A to G, chromosome 9 at 123,660,502 bp
  • C to T, chromosome 10 at 68,098,462 bp
  • T to C, chromosome 10 at 88,782,689 bp
  • T to A, chromosome 10 at 125,294,700 bp
  • T to C, chromosome 11 at 70,649,681 bp
  • C to T, chromosome 11 at 106,142,553 bp
  • T to A, chromosome 11 at 120,550,685 bp
  • T to C, chromosome 14 at 9,870,095 bp
  • A to G, chromosome 14 at 54,907,237 bp
  • C to A, chromosome 15 at 74,881,614 bp
  • T to C, chromosome 19 at 5,884,546 bp
  • T to A, chromosome 19 at 17,124,348 bp
  • C to T, chromosome 19 at 42,090,548 bp
  • T to C, chromosome 19 at 42,267,071 bp
  • T to A, chromosome 19 at 46,136,109 bp
  • T to C, chromosome 19 at 46,590,196 bp
  • T to C, chromosome X at 13,128,390 bp
  • T to C, chromosome X at 60,135,446 bp
  • T to A, chromosome X at 74,307,808 bp
  • T to A, chromosome X at 104,483,328 bp
  • A to C, chromosome Y at 1,158,512 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3547 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040666-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.