Strain Name:
C57BL/6J-MtgxR3616Btlr/Mmmh
Stock Number:
040673-MU
Citation ID:
RRID:MMRRC_040673-MU
Other Names:
R3616 (G1), C57BL/6J-MtgxR3616Btlr
Major Collection:

Gene Information

Espl1
Name: extra spindle pole bodies 1, separase
Synonyms: PRCE, separase, SSE, ESP1, PRCE, Cerp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 105988
VEGA: 15
Homologene: 32151
Sntb2
Name: syntrophin, basic 2
Synonyms: Snt2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 20650
Homologene: 4911
Fiz1
Name: Flt3 interacting zinc finger protein 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 23877
Homologene: 22663
Hsp90aa1
Name: heat shock protein 90, alpha (cytosolic), class A member 1
Synonyms: hsp4, Hsp90, Hsp86-1, Hsp89, Hspca
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 15519
HGNC: HGNC:5253
Homologene: 68464
Barhl1
Name: BarH like homeobox 1
Synonyms: Dres115, MBH2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 54422
HGNC: HGNC:953
Homologene: 81871
Ap2b1
Name: adaptor-related protein complex 2, beta 1 subunit
Synonyms: 1300012O03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 71770
HGNC: HGNC:563
Homologene: 137384
Gse1
Name: genetic suppressor element 1, coiled-coil protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 382034
Homologene: 40964
Kif1b
Name: kinesin family member 1B
Synonyms: D4Mil1e, Kif1b alpha, Kif1b beta, KIF1Bp130, KIF1Bp204, N-3 kinesin, A530096N05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 16561
Homologene: 99835
Aqr
Name: aquarius
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 11834
Homologene: 7629
Foxi2
Name: forkhead box I2
Synonyms: B130055A05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 270004
Homologene: 18838
Efs
Name: embryonal Fyn-associated substrate
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 13644
VEGA: 14
Homologene: 4284
Pard6b
Name: par-6 family cell polarity regulator beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 58220
Homologene: 23302
Psap
Name: prosaposin
Synonyms: SGP-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 19156
HGNC: HGNC:9498
Homologene: 37680
Dzip3
Name: DAZ interacting protein 3, zinc finger
Synonyms: 6430549P11Rik, 2310047C04Rik, 2A-HUB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 224170
Homologene: 8771
Slc16a1
Name: solute carrier family 16 (monocarboxylic acid transporters), member 1
Synonyms: MCT1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20501
Homologene: 20662
Tusc3
Name: tumor suppressor candidate 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 80286
Homologene: 6937
Smg5
Name: SMG5 nonsense mediated mRNA decay factor
Synonyms: Smg-5 homolog, nonsense mediated mRNA decay factor (C. elegans)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229512
Homologene: 9095
Tmem231
Name: transmembrane protein 231
Synonyms: 4932417I16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234740
Homologene: 78035
Tdo2
Name: tryptophan 2,3-dioxygenase
Synonyms: TDO, TO, chky
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 56720
Homologene: 4132
Txnip
Name: thioredoxin interacting protein
Synonyms: THIF, VDUP1, mVDUP1, Hyplip1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 56338
Homologene: 38186
Col28a1
Name: collagen, type XXVIII, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 213945
Homologene: 66345
Nlrp12
Name: NLR family, pyrin domain containing 12
Synonyms: Nalp12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 378425
Homologene: 16972
Grik5
Name: glutamate receptor, ionotropic, kainate 5 (gamma 2)
Synonyms: KA2, GluRgamma2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 14809
HGNC: HGNC:4583
Homologene: 1578
Nlrp10
Name: NLR family, pyrin domain containing 10
Synonyms: 6430548I20Rik, Pynod, Nalp10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 244202
Homologene: 18468
Miip
Name: migration and invasion inhibitory protein
Synonyms: D4Wsu114e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 28010
Homologene: 15879
Dpysl2
Name: dihydropyrimidinase-like 2
Synonyms: Ulip2, Crmp2, DRP2, TOAD-64
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 12934
HGNC: HGNC:3014
Homologene: 74392
Gdf2
Name: growth differentiation factor 2
Synonyms: Bmp9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 12165
VEGA: 14
HGNC: HGNC:4217
Homologene: 32299
Olfr142
Name: olfactory receptor 142
Synonyms: K20, MOR227-2, GA_x6K02T2Q125-51607674-51606757
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 406186
Homologene: 45081
Pla2g2e
Name: phospholipase A2, group IIE
Synonyms: mGIIEsPLA2s
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 26970
Homologene: 8083
Gm5105
Name: predicted gene 5105
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 329763
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 2 at 28,911,550 bp
  • T to C, chromosome 2 at 90,252,409 bp
  • A to T, chromosome 2 at 114,136,887 bp
  • T to C, chromosome 2 at 168,087,339 bp
  • A to G, chromosome 3 at 81,975,428 bp
  • A to G, chromosome 3 at 88,336,451 bp
  • C to G, chromosome 3 at 96,559,447 bp
  • T to A, chromosome 3 at 104,653,570 bp
  • C to A, chromosome 3 at 138,049,688 bp
  • G to A, chromosome 4 at 138,880,374 bp
  • A to G, chromosome 4 at 147,865,914 bp
  • A to T, chromosome 4 at 149,262,283 bp
  • A to G, chromosome 6 at 8,014,942 bp
  • T to A, chromosome 7 at 3,240,575 bp
  • A to G, chromosome 7 at 5,008,172 bp
  • C to T, chromosome 7 at 25,022,571 bp
  • A to G, chromosome 7 at 108,924,476 bp
  • T to A, chromosome 7 at 135,410,451 bp
  • A to T, chromosome 8 at 39,164,838 bp
  • T to A, chromosome 8 at 106,981,351 bp
  • C to T, chromosome 8 at 111,918,313 bp
  • C to G, chromosome 8 at 120,572,742 bp
  • A to G, chromosome 10 at 60,294,603 bp
  • T to A, chromosome 11 at 83,324,565 bp
  • T to A, chromosome 12 at 110,695,680 bp
  • C to A, chromosome 12 at 110,695,681 bp
  • G to A, chromosome 14 at 33,944,957 bp
  • T to C, chromosome 14 at 54,920,095 bp
  • T to A, chromosome 14 at 66,834,370 bp
  • A to G, chromosome 15 at 102,312,989 bp
  • A to G, chromosome 16 at 48,937,063 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3616 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040673-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.