Strain Name:
C57BL/6J-MtgxR4018Btlr/Mmmh
Stock Number:
040848-MU
Citation ID:
RRID:MMRRC_040848-MU
Other Names:
R4018 (G1), C57BL/6J-MtgxR4018Btlr
Major Collection:

Gene Information

Notch2
Name: notch 2
Synonyms: Motch B, N2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 18129
HGNC: HGNC:7882
Homologene: 7865
Septin4
Name: septin 4
Synonyms: cell division control-related protein 2b, Pnutl2, Bh5, septin H5, ARTS, Sept4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18952
HGNC: HGNC:9165
Homologene: 6107
Odf2l
Name: outer dense fiber of sperm tails 2-like
Synonyms: 4733401D09Rik, 9630045K08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 52184
Homologene: 12011
Llgl2
Name: LLGL2 scribble cell polarity complex component
Synonyms: 9130006H11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217325
HGNC: HGNC:6629
Homologene: 3323
Prkca
Name: protein kinase C, alpha
Synonyms: Pkca
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18750
HGNC: HGNC:9393
Homologene: 55679
Polr2a
Name: polymerase (RNA) II (DNA directed) polypeptide A
Synonyms: 220kDa, Rpo2-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 20020
HGNC: HGNC:9187
Homologene: 721
Usp34
Name: ubiquitin specific peptidase 34
Synonyms: A530081C03Rik, Murr2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17847
Homologene: 40978
Fntb
Name: farnesyltransferase, CAAX box, beta
Synonyms: 2010013E13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 110606
HGNC: HGNC:3785
Homologene: 1535
Ryr2
Name: ryanodine receptor 2, cardiac
Synonyms: 9330127I20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20191
VEGA: 13
Homologene: 37423
Iws1
Name: IWS1, SUPT6 interacting protein
Synonyms: 1700069O15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 73473
Homologene: 134421
Cd300lg
Name: CD300 molecule like family member G
Synonyms: 2310016B05Rik, Clm9, D11Ertd736e, nepmucin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 52685
Homologene: 17059
Maml1
Name: mastermind like transcriptional coactivator 1
Synonyms: Mam-1, D930008C07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 103806
Homologene: 8845
Edem3
Name: ER degradation enhancer, mannosidase alpha-like 3
Synonyms: 2310050N11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 66967
Homologene: 11866
Brip1
Name: BRCA1 interacting protein C-terminal helicase 1
Synonyms: BACH1, 8030460J03Rik, 3110009N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237911
Homologene: 32766
Tsc2
Name: TSC complex subunit 2
Synonyms: tuberin, Nafld, tuberous sclerosis 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 22084
VEGA: 17
Homologene: 462
Ldb3
Name: LIM domain binding 3
Synonyms: cypher, ZASP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 24131
VEGA: 14
Homologene: 134626
Mlxipl
Name: MLX interacting protein-like
Synonyms: WS-bHLH, ChREBP, Wbscr14, bHLHd14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 58805
Homologene: 32507
Kdm5d
Name: lysine (K)-specific demethylase 5D
Synonyms: Smcy, Jarid1d, HY
Type: Gene
Species: Mus musculus (mouse)
Chromosome: Y
NCBI: 20592
Homologene: 55838
Vmn2r6
Name: vomeronasal 2, receptor 6
Synonyms: EG620718, EG667069
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 667069
Homologene: 129754
Oc90
Name: otoconin 90
Synonyms: PLA2L, Ocn-95, Pla2ll
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 18256
HGNC: HGNC:8100
Homologene: 19021
Celsr2
Name: cadherin, EGF LAG seven-pass G-type receptor 2
Synonyms: mfmi1, flamingo, EGFL2, Adgrc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 53883
HGNC: HGNC:3231
Homologene: 1078
Scyl3
Name: SCY1-like 3 (S. cerevisiae)
Synonyms: Pace1, 1200016D23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 240880
Homologene: 10706
Slc25a39
Name: solute carrier family 25, member 39
Synonyms: 3010027G13Rik, D11Ertd333e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 68066
Homologene: 69200
Wdr66
Name: WD repeat domain 66
Synonyms: 4930415N18Rik, 4933428F06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 269701
Homologene: 16964
Olfr1453
Name: olfactory receptor 1453
Synonyms: GA_x6K02T2RE5P-3357666-3356743, MOR202-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 258695
Homologene: 120159
Ranbp3l
Name: RAN binding protein 3-like
Synonyms: C130037N17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223332
VEGA: 15
Homologene: 35407
Slc9a9
Name: solute carrier family 9 (sodium/hydrogen exchanger), member 9
Synonyms: 5730527A11Rik, Nhe9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 331004
Homologene: 69753
Endou
Name: endonuclease, polyU-specific
Synonyms: Tcl-30, Pp11r
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 19011
VEGA: 15
Homologene: 48394
Cd300ld2
Name: CD300 molecule like family member D2
Synonyms: Gm11709
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 629970
Gm5431
Name: predicted gene 5431
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 432555
Rab3c
Name: RAB3C, member RAS oncogene family
Synonyms: 3110037E15Rik, 2700062I01Rik, 3110015B08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 67295
VEGA: 13
Homologene: 23420
Aga
Name: aspartylglucosaminidase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 11593
HGNC: HGNC:318
Homologene: 13
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 151,804,826 bp
  • A to G, chromosome 1 at 163,936,499 bp
  • T to C, chromosome 3 at 64,556,472 bp
  • G to T, chromosome 3 at 98,104,565 bp
  • C to T, chromosome 3 at 108,394,965 bp
  • G to A, chromosome 3 at 145,151,096 bp
  • A to G, chromosome 5 at 123,322,454 bp
  • T to C, chromosome 5 at 135,132,672 bp
  • A to G, chromosome 8 at 53,523,191 bp
  • T to C, chromosome 9 at 94,685,163 bp
  • C to T, chromosome 11 at 23,489,033 bp
  • T to A, chromosome 11 at 48,889,168 bp
  • A to T, chromosome 11 at 50,265,784 bp
  • T to C, chromosome 11 at 69,735,059 bp
  • G to T, chromosome 11 at 86,138,851 bp
  • G to A, chromosome 11 at 87,585,121 bp
  • A to G, chromosome 11 at 102,041,594 bp
  • A to T, chromosome 11 at 102,405,024 bp
  • T to A, chromosome 11 at 107,939,602 bp
  • G to T, chromosome 11 at 115,012,504 bp
  • A to G, chromosome 11 at 115,847,612 bp
  • C to A, chromosome 12 at 76,887,888 bp
  • T to A, chromosome 13 at 11,918,414 bp
  • T to A, chromosome 13 at 110,084,194 bp
  • T to C, chromosome 14 at 34,552,171 bp
  • T to C, chromosome 15 at 9,063,148 bp
  • T to C, chromosome 15 at 65,887,608 bp
  • T to A, chromosome 15 at 97,718,937 bp
  • T to C, chromosome 17 at 24,625,281 bp
  • C to A, chromosome 18 at 32,070,152 bp
  • T to C, chromosome 19 at 13,027,825 bp
  • T to C, chromosome Y at 910,441 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4018 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040848-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.