Strain Name:
C57BL/6J-MtgxR3966Btlr/Mmmh
Stock Number:
040935-MU
Citation ID:
RRID:MMRRC_040935-MU
Other Names:
R3966 (G1), C57BL/6J-MtgxR3966Btlr
Major Collection:

Gene Information

Nfatc2
Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2
Synonyms: NFAT1, NFATp, NFAT1-D
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18019
HGNC: HGNC:7776
Homologene: 7861
Nfat5
Name: nuclear factor of activated T cells 5
Synonyms: OREBP, TonEBP, nfatz, B130038B15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 54446
HGNC: HGNC:7774
Homologene: 4811
Npm1
Name: nucleophosmin 1
Synonyms: NO38, B23, nucleolar protein NO38
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18148
HGNC: HGNC:7910
Homologene: 81697
Ncor1
Name: nuclear receptor co-repressor 1
Synonyms: A230020K14Rik, N-CoR, 5730405M06Rik, Rxrip13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 20185
HGNC: HGNC:7672
Homologene: 38166
Cadps
Name: Ca2+-dependent secretion activator
Synonyms: CAPS1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 27062
VEGA: 14
HGNC: HGNC:1426
Homologene: 2755
Mbd5
Name: methyl-CpG binding domain protein 5
Synonyms: OTTMUSG00000012483, 9430004D19Rik, C030040A15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 109241
Homologene: 81861
Nucb2
Name: nucleobindin 2
Synonyms: Calnuc, NEFA, nesfatin-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 53322
HGNC: HGNC:8044
Homologene: 3676
Kdm4c
Name: lysine (K)-specific demethylase 4C
Synonyms: 2410141F18Rik, Jmjd2c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 76804
Homologene: 41004
Agap1
Name: ArfGAP with GTPase domain, ankyrin repeat and PH domain 1
Synonyms: Centg2, Ggap1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 347722
Homologene: 56689
Rnf38
Name: ring finger protein 38
Synonyms: Oip1, 2610202O07Rik, 1700065B19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 73469
Homologene: 32550
Mycbp2
Name: MYC binding protein 2, E3 ubiquitin protein ligase
Synonyms: Phr1, C130061D10Rik, Pam
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 105689
Homologene: 9005
Ctc1
Name: CTS telomere maintenance complex component 1
Synonyms: 1500010J02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 68964
Homologene: 11830
Brwd1
Name: bromodomain and WD repeat domain containing 1
Synonyms: D530019K20Rik, repro5, 5330419I02Rik, G1-403-16, Wdr9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 93871
Homologene: 23130
Tet2
Name: tet methylcytosine dioxygenase 2
Synonyms: E130014J05Rik, Ayu17-449
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 214133
Homologene: 49498
Usp24
Name: ubiquitin specific peptidase 24
Synonyms: 2810030C21Rik, 2700066K03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 329908
Homologene: 35420
Safb2
Name: scaffold attachment factor B2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224902
Homologene: 35210
Clptm1l
Name: CLPTM1-like
Synonyms: C130052I12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218335
VEGA: 13
Homologene: 12767
Sos1
Name: SOS Ras/Rac guanine nucleotide exchange factor 1
Synonyms: 4430401P03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 20662
VEGA: 17
Homologene: 4117
Mcpt1
Name: mast cell protease 1
Synonyms: Mcp-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 17224
VEGA: 14
Homologene: 137209
Chd2
Name: chromodomain helicase DNA binding protein 2
Synonyms: 2810040A01Rik, 2810013C04Rik, 5630401D06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 244059
HGNC: HGNC:1917
Homologene: 37462
Mcf2l
Name: mcf.2 transforming sequence-like
Synonyms: Dbs, C130040G20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 17207
Homologene: 11804
C3
Name: complement component 3
Synonyms: acylation stimulating protein, Plp, complement factor 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 12266
HGNC: HGNC:1318
Homologene: 68031
Cemip
Name: cell migration inducing protein, hyaluronan binding
Synonyms: 12H19.01.T7, 6330404C01Rik, Hybid, 9930013L23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 80982
Homologene: 10268
Cyp1a1
Name: cytochrome P450, family 1, subfamily a, polypeptide 1
Synonyms: cytochrome P450 subfamily I, polypeptide 1, P450-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 13076
VEGA: 9
HGNC: HGNC:2595
Homologene: 68062
Ms4a6b
Name: membrane-spanning 4-domains, subfamily A, member 6B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 69774
Homologene: 128306
Tom1
Name: target of myb1 trafficking protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 21968
Homologene: 88453
Ptgs2
Name: prostaglandin-endoperoxide synthase 2
Synonyms: PGHS-2, Pghs2, COX2, Tis10, prostaglandin G/H synthase, cyclooxygenase-2, Cox-2, cyclooxygenase 2, PHS-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 19225
HGNC: HGNC:9605
Homologene: 31000
Trp73
Name: transformation related protein 73
Synonyms: deltaNp73, p73, TAp73
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 22062
Homologene: 3960
Megf10
Name: multiple EGF-like-domains 10
Synonyms: LOC240312, 3000002B06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 70417
Homologene: 23771
Tmx4
Name: thioredoxin-related transmembrane protein 4
Synonyms: 2810417D04Rik, 4930500L08Rik, Txndc13, D2Bwg1356e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 52837
Homologene: 10901
Gprc6a
Name: G protein-coupled receptor, family C, group 6, member A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 210198
VEGA: 10
Homologene: 17529
Inhbb
Name: inhibin beta-B
Synonyms: activin beta-B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16324
HGNC: HGNC:6067
Homologene: 1654
Zfp628
Name: zinc finger protein 628
Synonyms: Zec
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 232816
Homologene: 72200
Nrap
Name: nebulin-related anchoring protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 18175
HGNC: HGNC:7988
Homologene: 4499
Kcnb1
Name: potassium voltage gated channel, Shab-related subfamily, member 1
Synonyms: Kv2.1, Kcr1-1, Shab
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16500
HGNC: HGNC:6231
Homologene: 37988
CAAA01221083.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Ehbp1l1
Name: EH domain binding protein 1-like 1
Synonyms: G430002G23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 114601
VEGA: 19
Homologene: 136059
Myrf
Name: myelin regulatory factor
Synonyms: LOC225908, Gm98, LOC386531
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 225908
HGNC: HGNC:1181
Homologene: 32167
Gm12169
Name: predicted gene 12169
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 210535
Homologene: 130033
Prkd1
Name: protein kinase D1
Synonyms: PKD1, Pkcm, Prkcm
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 18760
VEGA: 12
HGNC: HGNC:9407
Homologene: 55680
Ces1g
Name: carboxylesterase 1G
Synonyms: Ces1, Ces-1, Ses-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 12623
HGNC: HGNC:1863
Homologene: 137354
Gpr141b
Name: G protein-coupled receptor 141B
Synonyms: Karma, A530099J19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 319293
Homologene: 105930
CN725425
Name: cDNA sequence CN725425
Synonyms: Gm5807
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 545126
Spink10
Name: serine peptidase inhibitor, Kazal type 10
Synonyms: A230091H23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 328971
Homologene: 131225
E330034G19Rik
Name: RIKEN cDNA E330034G19 gene
Synonyms: ZPAC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 105418
Med31
Name: mediator complex subunit 31
Synonyms: l11Jus15, 3110004H13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 67279
Homologene: 9368
Gm14137
Name: predicted gene 14137
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 623781
Homologene: 85847
Vmn2r33
Name: vomeronasal 2, receptor 33
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 624512
Homologene: 113703
Pde2a
Name: phosphodiesterase 2A, cGMP-stimulated
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 207728
HGNC: HGNC:8777
Homologene: 1952
C1galt1c1
Name: C1GALT1-specific chaperone 1
Synonyms: Cosmc, 1500002I11Rik, C81205
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 59048
Homologene: 10993
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 89,834,461 bp
  • C to A, chromosome 1 at 119,417,561 bp
  • T to A, chromosome 1 at 150,105,475 bp
  • A to T, chromosome 2 at 49,272,070 bp
  • T to A, chromosome 2 at 119,175,016 bp
  • T to C, chromosome 2 at 134,600,061 bp
  • C to T, chromosome 2 at 167,104,492 bp
  • C to T, chromosome 2 at 168,504,549 bp
  • A to G, chromosome 3 at 133,487,657 bp
  • A to G, chromosome 4 at 44,129,664 bp
  • A to G, chromosome 4 at 74,298,583 bp
  • A to G, chromosome 4 at 106,410,769 bp
  • A to G, chromosome 4 at 154,062,036 bp
  • T to C, chromosome 7 at 4,921,745 bp
  • T to C, chromosome 7 at 7,554,169 bp
  • A to G, chromosome 7 at 73,464,395 bp
  • T to A, chromosome 7 at 83,951,509 bp
  • C to T, chromosome 7 at 101,501,133 bp
  • A to G, chromosome 7 at 116,528,875 bp
  • G to A, chromosome 7 at 141,699,664 bp
  • T to A, chromosome 8 at 13,017,337 bp
  • A to G, chromosome 8 at 75,059,239 bp
  • C to A, chromosome 8 at 93,328,511 bp
  • G to T, chromosome 8 at 107,367,289 bp
  • T to A, chromosome 9 at 57,700,149 bp
  • CAAA to CA, chromosome 10 at 51,615,680 bp
  • C to T, chromosome 11 at 33,160,350 bp
  • T to A, chromosome 11 at 46,535,513 bp
  • T to C, chromosome 11 at 62,344,757 bp
  • T to A, chromosome 11 at 69,031,128 bp
  • C to T, chromosome 11 at 72,211,929 bp
  • C to T, chromosome 12 at 50,392,941 bp
  • A to G, chromosome 13 at 19,729,444 bp
  • T to C, chromosome 13 at 73,615,972 bp
  • A to T, chromosome 14 at 12,522,161 bp
  • G to T, chromosome 14 at 24,306,871 bp
  • G to A, chromosome 14 at 56,019,046 bp
  • A to G, chromosome 14 at 103,138,725 bp
  • T to A, chromosome 15 at 91,242,687 bp
  • G to A, chromosome 16 at 96,044,530 bp
  • T to C, chromosome 17 at 56,575,356 bp
  • A to G, chromosome 17 at 57,218,664 bp
  • C to T, chromosome 17 at 80,455,179 bp
  • A to G, chromosome 18 at 57,180,574 bp
  • T to C, chromosome 18 at 62,657,904 bp
  • C to T, chromosome 19 at 5,710,573 bp
  • T to C, chromosome 19 at 10,219,615 bp
  • T to C, chromosome 19 at 11,521,734 bp
  • T to C, chromosome 19 at 56,342,144 bp
  • A to T, chromosome X at 38,631,576 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3966 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
040935-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.