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Strain Name:
C57BL/6J-MtgxR3977Btlr/Mmmh
Stock Number:
040940-MU
Citation ID:
RRID:MMRRC_040940-MU
Other Names:
R3977 (G1), C57BL/6J-MtgxR3977Btlr
Major Collection:

Strain Information

Lrig2
Name: leucine-rich repeats and immunoglobulin-like domains 2
Synonyms: 4632419I10Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 269473
Homologene: 8882
Frem2
Name: Fras1 related extracellular matrix protein 2
Synonyms: 8430406N05Rik, 6030440P17Rik, my, ne, b2b1562Clo
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 242022
Homologene: 18454
Rc3h1
Name: RING CCCH (C3H) domains 1
Synonyms: roquin, 5730557L09Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 381305
Homologene: 19036
Slco1a5
Name: solute carrier organic anion transporter family, member 1a5
Synonyms: Oatp3, Slc21a7
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 108096
Homologene: 56603
Bicral
Name: BRD4 interacting chromatin remodeling complex associated protein like
Synonyms: BC032203, Gltscr1l
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 210982
VEGA: 17
Homologene: 19366
Mcmbp
Name: minichromosome maintenance complex binding protein
Synonyms: 1110007A13Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 210711
Homologene: 11749
Brpf3
Name: bromodomain and PHD finger containing, 3
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 268936
Homologene: 16092
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 10,209,634 bp
  • C to G, chromosome 1 at 19,104,494 bp
  • A to T, chromosome 1 at 60,498,523 bp
  • A to T, chromosome 1 at 130,936,033 bp
  • A to G, chromosome 1 at 134,765,975 bp
  • A to G, chromosome 1 at 159,892,023 bp
  • A to G, chromosome 1 at 160,959,399 bp
  • T to G, chromosome 1 at 162,958,578 bp
  • T to A, chromosome 2 at 26,384,793 bp
  • C to A, chromosome 2 at 90,021,745 bp
  • C to T, chromosome 2 at 166,795,526 bp
  • A to G, chromosome 3 at 53,652,070 bp
  • A to G, chromosome 3 at 103,060,225 bp
  • A to G, chromosome 3 at 103,873,641 bp
  • A to T, chromosome 3 at 104,457,844 bp
  • A to G, chromosome 3 at 142,603,709 bp
  • G to C, chromosome 4 at 134,703,438 bp
  • T to C, chromosome 5 at 36,485,166 bp
  • C to T, chromosome 5 at 114,932,898 bp
  • C to A, chromosome 5 at 124,113,209 bp
  • C to T, chromosome 6 at 34,953,788 bp
  • A to G, chromosome 6 at 41,130,145 bp
  • A to G, chromosome 6 at 133,036,317 bp
  • A to G, chromosome 6 at 142,258,972 bp
  • T to C, chromosome 7 at 27,920,687 bp
  • T to C, chromosome 7 at 85,958,137 bp
  • T to C, chromosome 7 at 105,555,901 bp
  • G to A, chromosome 7 at 126,580,136 bp
  • A to G, chromosome 7 at 128,698,583 bp
  • C to A, chromosome 10 at 51,621,101 bp
  • A to G, chromosome 10 at 62,290,319 bp
  • A to T, chromosome 10 at 76,217,651 bp
  • A to G, chromosome 10 at 106,830,629 bp
  • C to T, chromosome 10 at 129,238,508 bp
  • T to C, chromosome 11 at 103,457,604 bp
  • C to T, chromosome 11 at 105,981,838 bp
  • G to A, chromosome 11 at 116,216,945 bp
  • T to A, chromosome 13 at 21,115,861 bp
  • A to G, chromosome 13 at 41,141,964 bp
  • G to T, chromosome 14 at 57,017,188 bp
  • A to G, chromosome 14 at 68,610,994 bp
  • A to G, chromosome 14 at 70,563,584 bp
  • T to C, chromosome 15 at 101,811,127 bp
  • T to C, chromosome 15 at 102,921,240 bp
  • T to A, chromosome 17 at 18,597,679 bp
  • T to A, chromosome 17 at 22,603,115 bp
  • A to G, chromosome 17 at 23,735,331 bp
  • A to G, chromosome 17 at 28,807,042 bp
  • T to C, chromosome 17 at 37,225,158 bp
  • T to A, chromosome 17 at 46,830,991 bp
  • C to A, chromosome 19 at 11,870,505 bp
  • T to A, chromosome 19 at 11,896,516 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R3977 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040940-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.