Strain Name:
C57BL/6J-MtgxR4065Btlr/Mmmh
Stock Number:
040972-MU
Citation ID:
RRID:MMRRC_040972-MU
Other Names:
R4065 (G1), C57BL/6J-MtgxR4065Btlr
Major Collection:

Gene Information

Vcan
Name: versican
Synonyms: PG-M, hdf, heart defect, 5430420N07Rik, DPEAAE, Cspg2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 13003
HGNC: HGNC:2464
Homologene: 3228
Adcy9
Name: adenylate cyclase 9
Synonyms: D16Wsu65e, ACtp10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 11515
HGNC: HGNC:240
Homologene: 868
Mga
Name: MAX gene associated
Synonyms: Mga, Mad5, C130042M01Rik, D030062C11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 29808
Homologene: 49351
Ssbp4
Name: single stranded DNA binding protein 4
Synonyms: 1210002E11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 76900
Homologene: 41881
Dnajc21
Name: DnaJ heat shock protein family (Hsp40) member C21
Synonyms: 9930116P15Rik, 4930461P20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 78244
Homologene: 6752
Usp47
Name: ubiquitin specific peptidase 47
Synonyms: 4930502N04Rik, A630020C16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 74996
Homologene: 9929
Mthfd1l
Name: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1-like
Synonyms: 2410004L15Rik, Fthfsdc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 270685
Homologene: 56706
Wdfy3
Name: WD repeat and FYVE domain containing 3
Synonyms: 2610509D04Rik, Ggtb3, D5Ertd66e, Bwf1, Bchs, Alfy
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 72145
Homologene: 22855
Dnajc7
Name: DnaJ heat shock protein family (Hsp40) member C7
Synonyms: mDj11, Ttc2, mTpr2, 2010003F24Rik, 2010004G07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 56354
Homologene: 68306
Ltn1
Name: listerin E3 ubiquitin protein ligase 1
Synonyms: 4930528H02Rik, Zfp294, Listerin, Rnf160
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 78913
VEGA: 16
Homologene: 32272
Foxj3
Name: forkhead box J3
Synonyms: C330039G02Rik, Fhd6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230700
Homologene: 35243
Spcs2
Name: signal peptidase complex subunit 2 homolog (S. cerevisiae)
Synonyms: 5730406I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 66624
Homologene: 8842
Utp6
Name: UTP6 small subunit processome component
Synonyms: HCA66, 4732497O03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216987
Homologene: 41265
Uhrf1
Name: ubiquitin-like, containing PHD and RING finger domains, 1
Synonyms: ICBP90, Np95
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 18140
Homologene: 7866
Plxna4
Name: plexin A4
Synonyms: Plxa4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243743
HGNC: HGNC:9102
Homologene: 77587
C2cd5
Name: C2 calcium-dependent domain containing 5
Synonyms: C030008B15Rik, CDP138, 5730419I09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 74741
Homologene: 8873
Dync2i2
Name: dynein 2 intermediate chain 2
Synonyms: 3200002I06Rik, Wdr34
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 71820
Homologene: 14156
Dhx29
Name: DEAH (Asp-Glu-Ala-His) box polypeptide 29
Synonyms: E130202M19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218629
VEGA: 13
Homologene: 10387
Camk1d
Name: calcium/calmodulin-dependent protein kinase ID
Synonyms: CKLiK, E030025C11Rik, A630059D12Rik, CaMKIdelta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 227541
Homologene: 121858
Rnps1-ps
Name: RNA binding protein with serine rich domain 1, pseudogene
Synonyms: Gm9825
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 100040661
Sf3a1
Name: splicing factor 3a, subunit 1
Synonyms: 1200014H24Rik, 5930416L09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 67465
Homologene: 4294
Trpc5
Name: transient receptor potential cation channel, subfamily C, member 5
Synonyms: CCE2, TRP5, Trrp5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 22067
Homologene: 74915
Muc4
Name: mucin 4
Synonyms: 4933405I11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 140474
HGNC: HGNC:7514
Homologene: 124469
Dnah12
Name: dynein, axonemal, heavy chain 12
Synonyms: Hdhc3, HL-19, DLP12, HL19, DHC3, LOC380889, 4921531P07Rik, Dnahc7l, Dnahc12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 110083
HGNC: HGNC:2943
Homologene: 56821
Lamc3
Name: laminin gamma 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 23928
HGNC: HGNC:6494
Homologene: 21222
Pcnx4
Name: pecanex homolog 4
Synonyms: 1810048J11Rik, Pcnxl4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 67708
VEGA: 12
Homologene: 23366
Ugcg
Name: UDP-glucose ceramide glucosyltransferase
Synonyms: GlcT-1, Epcs21, Ugcgl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 22234
Homologene: 37763
Fat4
Name: FAT atypical cadherin 4
Synonyms: 6030410K14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 329628
Homologene: 14377
Zfp654
Name: zinc finger protein 654
Synonyms: 1810008K20Rik, 1600021C16Rik, Gm5488
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 72020
Homologene: 10114
Fras1
Name: Fraser extracellular matrix complex subunit 1
Synonyms: bl, E130113P14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231470
Homologene: 23516
Abcc9
Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 9
Synonyms: SUR2B, SUR2A, Sur2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 20928
HGNC: HGNC:60
Homologene: 56521
Fshr
Name: follicle stimulating hormone receptor
Synonyms: follicle-stimulating hormone receptor, Follitropin receptor, FSH-R
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 14309
VEGA: 17
HGNC: HGNC:3969
Homologene: 117
Gm11437
Name: predicted gene 11437
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 628813
Homologene: 82346
Atg2a
Name: autophagy related 2A
Synonyms: 1810013C15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 329015
Homologene: 86985
Ppm1d
Name: protein phosphatase 1D magnesium-dependent, delta isoform
Synonyms: Wip1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 53892
HGNC: HGNC:9277
Homologene: 31185
Mvp
Name: major vault protein
Synonyms: VAULT1, LRP, 2310009M24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 78388
HGNC: HGNC:7531
Homologene: 3752
Olfr225
Name: olfactory receptor 225
Synonyms: GA_x6K02T0073K-490-1545, MOR256-51
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 257886
Homologene: 105324
Man1c1
Name: mannosidase, alpha, class 1C, member 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230815
Homologene: 69303
Car6
Name: carbonic anhydrase 6
Synonyms: DOC1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 12353
HGNC: HGNC:1380
Homologene: 20324
Ttll11
Name: tubulin tyrosine ligase-like family, member 11
Synonyms: 4932702F08Rik, D2Ertd624e, 4933424A20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74410
Homologene: 77534
Prb1
Name: proline-rich protein BstNI subfamily 1
Synonyms: proline-rich proteoglycan 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 381833
HGNC: HGNC:9338
B430305J03Rik
Name: RIKEN cDNA B430305J03 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Olfr972
Name: olfactory receptor 972
Synonyms: GA_x6K02T2PVTD-33572803-33573747, MOR171-17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258603
VEGA: 9
Homologene: 121532
Cant1
Name: calcium activated nucleotidase 1
Synonyms: 5830420C20Rik, Apy1h, Shapy, D11Bwg0554e, SCAN-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 76025
Homologene: 69460
Hecw1
Name: HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1
Synonyms: NEDL1, E130207I19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 94253
VEGA: 13
Homologene: 9004
Irx2
Name: Iroquois homeobox 2
Synonyms: IRX6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 16372
Homologene: 56490
Olfr541
Name: olfactory receptor 541
Synonyms: GA_x6K02T2PBJ9-42850324-42851256, MOR253-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258964
Homologene: 138322
Grem1
Name: gremlin 1, DAN family BMP antagonist
Synonyms: gremlin, Drm, Cktsf1b1, Grem
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 23892
HGNC: HGNC:2001
Homologene: 8022
Zfp783
Name: zinc finger protein 783
Synonyms: ENSMUSG00000072653, A230106D06Rik, Gm20494
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232785
Fbxo16
Name: F-box protein 16
Synonyms: Fbx16
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 50759
VEGA: 14
Homologene: 9273
Gm12258
Name: predicted gene 12258
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 108167848
4933434E20Rik
Name: RIKEN cDNA 4933434E20 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 99650
Homologene: 41041
Uros
Name: uroporphyrinogen III synthase
Synonyms: hydroxymethylbilane hydrolyase (cyclizing), URO-synthase, uroporphyrinogen-III synthase, Uros3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22276
Homologene: 37296
Gm17108
Name: predicted gene 17108
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 2 at 5,565,773 bp
  • A to G, chromosome 2 at 30,032,808 bp
  • C to A, chromosome 2 at 31,945,258 bp
  • G to A, chromosome 2 at 35,902,789 bp
  • A to G, chromosome 2 at 113,749,688 bp
  • T to C, chromosome 2 at 119,947,002 bp
  • A to T, chromosome 3 at 39,009,197 bp
  • A to G, chromosome 3 at 61,364,225 bp
  • C to T, chromosome 3 at 90,058,766 bp
  • C to T, chromosome 4 at 59,207,798 bp
  • T to C, chromosome 4 at 119,610,009 bp
  • G to C, chromosome 4 at 134,703,438 bp
  • G to A, chromosome 4 at 150,197,459 bp
  • T to A, chromosome 5 at 96,770,683 bp
  • C to T, chromosome 5 at 101,922,447 bp
  • T to A, chromosome 6 at 7,983,009 bp
  • T to A, chromosome 6 at 32,236,365 bp
  • A to T, chromosome 6 at 47,945,565 bp
  • T to A, chromosome 6 at 132,207,695 bp
  • T to C, chromosome 6 at 142,645,890 bp
  • A to G, chromosome 6 at 143,073,671 bp
  • A to G, chromosome 7 at 99,844,805 bp
  • A to G, chromosome 7 at 112,053,416 bp
  • A to G, chromosome 7 at 126,996,317 bp
  • A to G, chromosome 7 at 133,702,328 bp
  • T to C, chromosome 7 at 140,704,269 bp
  • T to C, chromosome 8 at 70,599,560 bp
  • T to C, chromosome 9 at 39,873,422 bp
  • A to G, chromosome 10 at 4,032,242 bp
  • T to A, chromosome 11 at 4,167,824 bp
  • C to T, chromosome 11 at 58,858,526 bp
  • T to C, chromosome 11 at 59,614,040 bp
  • T to C, chromosome 11 at 79,946,247 bp
  • A to G, chromosome 11 at 84,164,511 bp
  • A to G, chromosome 11 at 85,345,852 bp
  • T to C, chromosome 11 at 100,601,781 bp
  • G to T, chromosome 11 at 118,407,997 bp
  • T to C, chromosome 12 at 72,556,360 bp
  • T to C, chromosome 13 at 14,316,431 bp
  • T to C, chromosome 13 at 69,612,460 bp
  • T to C, chromosome 13 at 72,632,539 bp
  • T to C, chromosome 13 at 89,679,887 bp
  • T to A, chromosome 13 at 112,964,742 bp
  • A to G, chromosome 14 at 26,770,448 bp
  • T to A, chromosome 14 at 65,270,829 bp
  • A to G, chromosome 15 at 10,451,553 bp
  • C to A, chromosome 16 at 4,288,434 bp
  • A to T, chromosome 16 at 32,751,051 bp
  • G to A, chromosome 16 at 64,785,925 bp
  • T to C, chromosome 16 at 87,416,230 bp
  • T to A, chromosome 17 at 56,318,020 bp
  • T to C, chromosome 17 at 88,985,966 bp
  • T to C, chromosome 19 at 6,258,366 bp
  • T to A, chromosome X at 144,419,598 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4065 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040972-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.