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Strain Name:
C57BL/6J-MtgxR4066Btlr/Mmmh
Stock Number:
040973-MU
Citation ID:
RRID:MMRRC_040973-MU
Other Names:
R4066 (G1), C57BL/6J-MtgxR4066Btlr
Major Collection:

Strain Information

Dspp
Name: dentin sialophosphoprotein
Synonyms: Dmp3, Dpp, Dsp
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 666279
HGNC: HGNC:3054
Adam23
Name: a disintegrin and metallopeptidase domain 23
Synonyms: MDC3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 23792
HGNC: HGNC:202
Homologene: 2826
Wdfy3
Name: WD repeat and FYVE domain containing 3
Synonyms: Ggtb3, 2610509D04Rik, D5Ertd66e, Bwf1, Bchs, Alfy
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 72145
Homologene: 22855
Ltn1
Name: listerin E3 ubiquitin protein ligase 1
Synonyms: 4930528H02Rik, Zfp294, Listerin, Rnf160
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 78913
VEGA: 16
Homologene: 32272
Recql4
Name: RecQ protein-like 4
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 79456
VEGA: 15
HGNC: HGNC:9949
Homologene: 3144
Kat7
Name: K(lysine) acetyltransferase 7
Synonyms: Hbo1, Hboa, Myst2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 217127
Homologene: 5134
Hnrnpr
Name: heterogeneous nuclear ribonucleoprotein R
Synonyms: hnRNPR, 2610003J05Rik, Hnrpr, 2610528B01Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 74326
HGNC: HGNC:5047
Homologene: 4251
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 16,394,059 bp
  • A to T, chromosome 1 at 63,563,425 bp
  • A to G, chromosome 1 at 81,241,835 bp
  • A to G, chromosome 1 at 92,491,189 bp
  • A to G, chromosome 1 at 98,463,846 bp
  • A to G, chromosome 1 at 135,827,427 bp
  • C to T, chromosome 1 at 171,408,323 bp
  • T to A, chromosome 2 at 89,575,179 bp
  • T to C, chromosome 3 at 8,509,608 bp
  • A to T, chromosome 3 at 90,387,378 bp
  • AAT to AATGTATATATAT, chromosome 3 at 118,897,089 bp
  • C to T, chromosome 4 at 59,207,798 bp
  • G to C, chromosome 4 at 134,703,438 bp
  • C to A, chromosome 4 at 136,339,346 bp
  • G to A, chromosome 4 at 150,197,459 bp
  • C to T, chromosome 5 at 34,878,847 bp
  • T to C, chromosome 5 at 38,483,349 bp
  • T to A, chromosome 5 at 96,770,683 bp
  • C to T, chromosome 5 at 101,922,447 bp
  • T to A, chromosome 5 at 104,177,194 bp
  • A to G, chromosome 5 at 136,695,450 bp
  • G to T, chromosome 5 at 144,556,312 bp
  • T to A, chromosome 6 at 7,983,009 bp
  • T to C, chromosome 6 at 130,188,744 bp
  • T to C, chromosome 7 at 79,412,757 bp
  • A to G, chromosome 7 at 111,046,301 bp
  • A to G, chromosome 8 at 4,748,716 bp
  • T to A, chromosome 9 at 38,488,482 bp
  • T to C, chromosome 10 at 88,675,683 bp
  • C to T, chromosome 11 at 58,858,526 bp
  • T to C, chromosome 11 at 59,614,040 bp
  • T to C, chromosome 11 at 95,284,141 bp
  • T to A, chromosome 12 at 77,464,061 bp
  • T to G, chromosome 12 at 111,979,511 bp
  • T to C, chromosome 13 at 14,316,431 bp
  • A to T, chromosome 14 at 44,285,638 bp
  • G to T, chromosome 14 at 55,767,495 bp
  • G to T, chromosome 14 at 57,588,054 bp
  • A to G, chromosome 14 at 59,547,005 bp
  • A to G, chromosome 15 at 76,705,827 bp
  • A to T, chromosome 16 at 32,751,051 bp
  • A to C, chromosome 16 at 32,753,917 bp
  • T to C, chromosome 16 at 87,416,230 bp
  • A to G, chromosome 17 at 31,443,838 bp
  • A to T, chromosome 17 at 55,971,581 bp
  • C to A, chromosome 17 at 71,716,238 bp
  • A to G, chromosome 18 at 78,111,377 bp
  • C to T, chromosome 19 at 4,270,360 bp
  • T to C, chromosome 19 at 6,229,330 bp
  • T to C, chromosome 19 at 33,720,859 bp
  • T to A, chromosome X at 144,419,598 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4066 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040973-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.