Strain Name:
C57BL/6J-MtgxR4066Btlr/Mmmh
Stock Number:
040973-MU
Citation ID:
RRID:MMRRC_040973-MU
Other Names:
R4066 (G1), C57BL/6J-MtgxR4066Btlr
Major Collection:

Gene Information

Dspp
Name: dentin sialophosphoprotein
Synonyms: Dmp3, Dpp, Dsp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 666279
HGNC: HGNC:3054
Adam23
Name: a disintegrin and metallopeptidase domain 23
Synonyms: MDC3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 23792
HGNC: HGNC:202
Homologene: 2826
Wdfy3
Name: WD repeat and FYVE domain containing 3
Synonyms: Ggtb3, 2610509D04Rik, D5Ertd66e, Bwf1, Bchs, Alfy
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 72145
Homologene: 22855
Ltn1
Name: listerin E3 ubiquitin protein ligase 1
Synonyms: 4930528H02Rik, Zfp294, Listerin, Rnf160
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 78913
VEGA: 16
Homologene: 32272
Recql4
Name: RecQ protein-like 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 79456
VEGA: 15
HGNC: HGNC:9949
Homologene: 3144
Kat7
Name: K(lysine) acetyltransferase 7
Synonyms: Hbo1, Hboa, Myst2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217127
Homologene: 5134
Hnrnpr
Name: heterogeneous nuclear ribonucleoprotein R
Synonyms: hnRNPR, 2610003J05Rik, Hnrpr, 2610528B01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 74326
HGNC: HGNC:5047
Homologene: 4251
Xpo4
Name: exportin 4
Synonyms: B430309A01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 57258
Homologene: 10733
Ctr9
Name: CTR9 homolog, Paf1/RNA polymerase II complex component
Synonyms: Tsp, Tsbp, Sh2bp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22083
Homologene: 40668
Nptx2
Name: neuronal pentraxin 2
Synonyms: np2, narp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 53324
HGNC: HGNC:7953
Homologene: 1892
Htt
Name: huntingtin
Synonyms: huntingtin, HD, htt, IT15, C430023I11Rik, Hdh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 15194
HGNC: HGNC:4851
Homologene: 1593
Stmn2
Name: stathmin-like 2
Synonyms: SCG10, Scgn10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20257
Homologene: 5102
Stau2
Name: staufen double-stranded RNA binding protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 29819
Homologene: 8666
Fut8
Name: fucosyltransferase 8
Synonyms: alpha (1,6) fucosyltransferase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 53618
VEGA: 12
HGNC: HGNC:4019
Homologene: 9650
Cab39l
Name: calcium binding protein 39-like
Synonyms: 2810425O13Rik, 1500031K13Rik, MO2L, 4930520C08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 69008
VEGA: 14
Homologene: 69422
Rnps1-ps
Name: RNA binding protein with serine rich domain 1, pseudogene
Synonyms: Gm9825
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 100040661
Ppp2r5b
Name: protein phosphatase 2, regulatory subunit B', beta
Synonyms: B'beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 225849
HGNC: HGNC:9310
Homologene: 38157
Trpc5
Name: transient receptor potential cation channel, subfamily C, member 5
Synonyms: CCE2, TRP5, Trrp5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 22067
Homologene: 74915
Arhgap30
Name: Rho GTPase activating protein 30
Synonyms: 6030405P05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226652
Homologene: 18766
Muc4
Name: mucin 4
Synonyms: 4933405I11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 140474
HGNC: HGNC:7514
Homologene: 124469
Nyap2
Name: neuronal tyrosine-phophorylated phosphoinositide 3-kinase adaptor 2
Synonyms: Jr6, 9430031J16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 241134
Homologene: 18239
Ugcg
Name: UDP-glucose ceramide glucosyltransferase
Synonyms: GlcT-1, Epcs21, Ugcgl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 22234
Homologene: 37763
Dpyd
Name: dihydropyrimidine dehydrogenase
Synonyms: DPD, E330028L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 99586
HGNC: HGNC:3012
Homologene: 85
Pde9a
Name: phosphodiesterase 9A
Synonyms: PDE9A1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 18585
HGNC: HGNC:8795
Homologene: 113565
Olfr1245
Name: olfactory receptor 1245
Synonyms: GA_x6K02T2Q125-51020951-51020028, MOR231-12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258784
Homologene: 121560
Spic
Name: Spi-C transcription factor (Spi-1/PU.1 related)
Synonyms: Spi-C, Prf, C76795
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 20728
Homologene: 7953
Fras1
Name: Fraser extracellular matrix complex subunit 1
Synonyms: bl, E130113P14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231470
Homologene: 23516
Shcbp1
Name: Shc SH2-domain binding protein 1
Synonyms: mPAL
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 20419
Homologene: 32123
Slc2a9
Name: solute carrier family 2 (facilitated glucose transporter), member 9
Synonyms: Glut9, SLC2A9B, SLC2a9A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 117591
Homologene: 69290
Olfr225
Name: olfactory receptor 225
Synonyms: GA_x6K02T0073K-490-1545, MOR256-51
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 257886
Homologene: 105324
Lipo2
Name: lipase, member O2
Synonyms: Gm8981
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 101055671
Homologene: 103863
Man1c1
Name: mannosidase, alpha, class 1C, member 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230815
Homologene: 69303
Togaram2
Name: TOG array regulator of axonemal microtubules 2
Synonyms: 4632412N22Rik, Fam179a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 320159
Homologene: 65315
Car6
Name: carbonic anhydrase 6
Synonyms: DOC1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 12353
HGNC: HGNC:1380
Homologene: 20324
Slco6d1
Name: solute carrier organic anion transporter family, member 6d1
Synonyms: 4921511I05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 70866
Homologene: 86643
Klra9
Name: killer cell lectin-like receptor subfamily A, member 9
Synonyms: Ly49I, LY49I1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16640
Homologene: 110821
Olfr1415
Name: olfactory receptor 1415
Synonyms: GA_x6K02T2R7CC-81266841-81267776, MOR103-12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 258228
Homologene: 79367
Hecw1
Name: HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1
Synonyms: NEDL1, E130207I19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 94253
VEGA: 13
Homologene: 9004
Slc27a3
Name: solute carrier family 27 (fatty acid transporter), member 3
Synonyms: fatty acid transport protein 3, FATP3, Acsvl3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 26568
Homologene: 11529
Ankrd13d
Name: ankyrin repeat domain 13 family, member D
Synonyms: 0710001P18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 68423
Homologene: 27612
Rd3l
Name: retinal degeneration 3-like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217874
Homologene: 19151
Gm8220
Name: predicted gene 8220
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 666660
Homologene: 128452
Olfr906
Name: olfactory receptor 906
Synonyms: GA_x6K02T2PVTD-32194085-32195020, MOR167-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258799
VEGA: 9
Homologene: 132439
Ltb4r1
Name: leukotriene B4 receptor 1
Synonyms: mBLTR, BLTR, BLT1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 16995
HGNC: HGNC:6713
Homologene: 22477
Gm12258
Name: predicted gene 12258
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 108167848
Lad1
Name: ladinin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16763
HGNC: HGNC:6472
Homologene: 4059
Shd
Name: src homology 2 domain-containing transforming protein D
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 20420
VEGA: 17
Homologene: 7537
Slc14a1
Name: solute carrier family 14 (urea transporter), member 1
Synonyms: 2610507K20Rik, UT-B, 3021401A05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 108052
Homologene: 9285
Myl10
Name: myosin, light chain 10, regulatory
Synonyms: PLRLC-C, PLRLC-B, PLRLC-A, PLRLC, 1700027I08Rik, Mylc2pl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 59310
Homologene: 69341
Gm16017
Name: predicted gene 16017
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 102633841
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 16,394,059 bp
  • A to T, chromosome 1 at 63,563,425 bp
  • A to G, chromosome 1 at 81,241,835 bp
  • A to G, chromosome 1 at 92,491,189 bp
  • A to G, chromosome 1 at 98,463,846 bp
  • A to G, chromosome 1 at 135,827,427 bp
  • C to T, chromosome 1 at 171,408,323 bp
  • T to A, chromosome 2 at 89,575,179 bp
  • T to C, chromosome 3 at 8,509,608 bp
  • A to T, chromosome 3 at 90,387,378 bp
  • AAT to AATGTATATATAT, chromosome 3 at 118,897,089 bp
  • C to T, chromosome 4 at 59,207,798 bp
  • G to C, chromosome 4 at 134,703,438 bp
  • C to A, chromosome 4 at 136,339,346 bp
  • G to A, chromosome 4 at 150,197,459 bp
  • C to T, chromosome 5 at 34,878,847 bp
  • T to C, chromosome 5 at 38,483,349 bp
  • T to A, chromosome 5 at 96,770,683 bp
  • C to T, chromosome 5 at 101,922,447 bp
  • T to A, chromosome 5 at 104,177,194 bp
  • A to G, chromosome 5 at 136,695,450 bp
  • G to T, chromosome 5 at 144,556,312 bp
  • T to A, chromosome 6 at 7,983,009 bp
  • T to C, chromosome 6 at 130,188,744 bp
  • T to C, chromosome 7 at 79,412,757 bp
  • A to G, chromosome 7 at 111,046,301 bp
  • A to G, chromosome 8 at 4,748,716 bp
  • T to A, chromosome 9 at 38,488,482 bp
  • T to C, chromosome 10 at 88,675,683 bp
  • C to T, chromosome 11 at 58,858,526 bp
  • T to C, chromosome 11 at 59,614,040 bp
  • T to C, chromosome 11 at 95,284,141 bp
  • T to A, chromosome 12 at 77,464,061 bp
  • T to G, chromosome 12 at 111,979,511 bp
  • T to C, chromosome 13 at 14,316,431 bp
  • A to T, chromosome 14 at 44,285,638 bp
  • G to T, chromosome 14 at 55,767,495 bp
  • G to T, chromosome 14 at 57,588,054 bp
  • A to G, chromosome 14 at 59,547,005 bp
  • A to G, chromosome 15 at 76,705,827 bp
  • A to T, chromosome 16 at 32,751,051 bp
  • A to C, chromosome 16 at 32,753,917 bp
  • T to C, chromosome 16 at 87,416,230 bp
  • A to G, chromosome 17 at 31,443,838 bp
  • A to T, chromosome 17 at 55,971,581 bp
  • C to A, chromosome 17 at 71,716,238 bp
  • A to G, chromosome 18 at 78,111,377 bp
  • C to T, chromosome 19 at 4,270,360 bp
  • T to C, chromosome 19 at 6,229,330 bp
  • T to C, chromosome 19 at 33,720,859 bp
  • T to A, chromosome X at 144,419,598 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4066 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
040973-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.