Strain Name:
C57BL/6J-MtgxR4239Btlr/Mmmh
Stock Number:
041056-MU
Citation ID:
RRID:MMRRC_041056-MU
Other Names:
R4239 (G1), C57BL/6J-MtgxR4239Btlr
Major Collection:

Gene Information

Nes
Name: nestin
Synonyms: Marc2, ESTM46, RC2, Ifaprc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 18008
HGNC: HGNC:7756
Homologene: 136487
Areg
Name: amphiregulin
Synonyms: Sdgf, AR, Mcub
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 11839
HGNC: HGNC:651
Homologene: 1252
Col18a1
Name: collagen, type XVIII, alpha 1
Synonyms: endostatin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 12822
HGNC: HGNC:2195
Homologene: 7673
Fbxl3
Name: F-box and leucine-rich repeat protein 3
Synonyms: Play68, Ovtm, Fbxl3a, Fbl3a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 50789
Homologene: 8127
Usp46
Name: ubiquitin specific peptidase 46
Synonyms: 1190009E20Rik, 2410018I08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 69727
Homologene: 56957
Psma7
Name: proteasome subunit alpha 7
Synonyms: C6-I
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 26444
HGNC: HGNC:9536
Homologene: 105299
Gtf2h1
Name: general transcription factor II H, polypeptide 1
Synonyms: 62kDa, p62
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 14884
HGNC: HGNC:4655
Homologene: 3885
Cog4
Name: component of oligomeric golgi complex 4
Synonyms: D8Ertd515e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 102339
Homologene: 7155
Dpp8
Name: dipeptidylpeptidase 8
Synonyms: 2310004I03Rik, 4932434F09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 74388
VEGA: 9
Homologene: 57098
Gfm2
Name: G elongation factor, mitochondrial 2
Synonyms: EFG2, 6530419G12Rik, MST027, A930009M04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 320806
Homologene: 6238
Boc
Name: biregional cell adhesion molecule-related/down-regulated by oncogenes (Cdon) binding protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 117606
Homologene: 32819
Zfp157
Name: zinc finger protein 157
Synonyms: A630094N24Rik, 2610020C11Rik, Roma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 72154
Homologene: 57009
Atad2b
Name: ATPase family, AAA domain containing 2B
Synonyms: D530031C13Rik, 1110014E10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 320817
VEGA: 12
Homologene: 86351
Slc35f5
Name: solute carrier family 35, member F5
Synonyms: 1300003P13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 74150
Homologene: 5745
Cfap46
Name: cilia and flagella associated protein 46
Synonyms: Ttc40, 9330101J02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 212124
C030017K20Rik
Name: RIKEN cDNA C030017K20 gene
Synonyms: LOC381581
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Large2
Name: LARGE xylosyl- and glucuronyltransferase 2
Synonyms: Gyltl1b, 5730485C17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228366
Homologene: 27362
Plcz1
Name: phospholipase C, zeta 1
Synonyms: 1700041H07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 114875
Homologene: 23815
Dnah3
Name: dynein, axonemal, heavy chain 3
Synonyms: Dnahc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 381917
HGNC: HGNC:2949
Homologene: 19674
Uba7
Name: ubiquitin-like modifier activating enzyme 7
Synonyms: Ube1l, 1300004C08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 74153
Homologene: 2502
Crip3
Name: cysteine-rich protein 3
Synonyms: TLP, TLP-A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 114570
Homologene: 28039
Otud7a
Name: OTU domain containing 7A
Synonyms: Otud7, Cezanne 2 protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 170711
Homologene: 15642
Trank1
Name: tetratricopeptide repeat and ankyrin repeat containing 1
Synonyms: C030048J01Rik, A230061D21Rik, Lba1, LOC235639
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 320429
Homologene: 45845
Wdpcp
Name: WD repeat containing planar cell polarity effector
Synonyms: homoloc-13, AV249152
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216560
Homologene: 9299
Phactr1
Name: phosphatase and actin regulator 1
Synonyms: 9630030F18Rik, Rpel1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218194
Homologene: 33597
Alpk2
Name: alpha-kinase 2
Synonyms: Hak
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225638
Homologene: 50475
Plxnb1
Name: plexin B1
Synonyms: 2900002G15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235611
HGNC: HGNC:9103
Homologene: 130508
Myo5c
Name: myosin VC
Synonyms: 9130003O20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 208943
VEGA: 9
HGNC: HGNC:7604
Homologene: 135711
Slc14a2
Name: solute carrier family 14 (urea transporter), member 2
Synonyms: UT-A5, UT-A3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 27411
VEGA: 18
Homologene: 5183
Prrt4
Name: proline-rich transmembrane protein 4
Synonyms: D330027H18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 101359
Homologene: 46116
Ehhadh
Name: enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase
Synonyms: L-PBE, MFP1, 1300002P22Rik, MFP, L-bifunctional enzyme, HD
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 74147
HGNC: HGNC:3247
Homologene: 1486
Ano5
Name: anoctamin 5
Synonyms: Gdd1, Tmem16e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233246
Homologene: 100071
Erbb2
Name: erb-b2 receptor tyrosine kinase 2
Synonyms: HER-2, ErbB-2, l11Jus8, HER2, c-erbB2, Neu oncogene, c-neu, Neu
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 13866
HGNC: HGNC:3430
Homologene: 3273
Serpinb6b
Name: serine (or cysteine) peptidase inhibitor, clade B, member 6b
Synonyms: Spi12, ovalbumin, NK13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20708
HGNC: HGNC:8950
Homologene: 22633
Upf3a
Name: UPF3 regulator of nonsense transcripts homolog A (yeast)
Synonyms: RENT3A, UPF3, 2600001C03Rik, 4930546M19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 67031
Homologene: 23395
Vmn2r14
Name: vomeronasal 2, receptor 14
Synonyms: EG231591
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231591
Homologene: 129606
Clip2
Name: CAP-GLY domain containing linker protein 2
Synonyms: Cyln2, WSCR4, CLIP-115
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 269713
HGNC: HGNC:2586
Homologene: 20718
Klk6
Name: kallikrein related-peptidase 6
Synonyms: protease M, Prss18, Bssp, Prss9, Klk29, neurosin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 19144
HGNC: HGNC:6367
Homologene: 68279
Gm6871
Name: predicted gene 6871
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 102642386
Ddi1
Name: DNA-damage inducible 1
Synonyms: 1700011N24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 71829
VEGA: 9
Homologene: 100549
B4galnt4
Name: beta-1,4-N-acetyl-galactosaminyl transferase 4
Synonyms: LOC381951
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 330671
Homologene: 27982
Fuca1
Name: fucosidase, alpha-L- 1, tissue
Synonyms: 9530055J05Rik, 0610006A03Rik, Afuc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 71665
HGNC: HGNC:4006
Homologene: 20078
Olfr539
Name: olfactory receptor 539
Synonyms: GA_x6K02T2PBJ9-42813436-42814368, MOR253-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258963
Homologene: 79393
Olfr583
Name: olfactory receptor 583
Synonyms: GA_x6K02T2PBJ9-5762668-5763618, MOR14-6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258752
Homologene: 133721
Prl7a1
Name: prolactin family 7, subfamily a, member 1
Synonyms: Prlpg, Prlpe, PLP-G, PLP-E
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 19113
Homologene: 136279
Ifi214
Name: interferon activated gene 214
Synonyms: BC094916, Pyhin-B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 545384
HGNC: HGNC:5395
Homologene: 115929
Olfr1002
Name: olfactory receptor 1002
Synonyms: GA_x6K02T2Q125-47127746-47126790, MOR175-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258566
Homologene: 104131
Speer4b
Name: spermatogenesis associated glutamate (E)-rich protein 4B
Synonyms: 1700081O22Rik, SPEER-4B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 73526
Homologene: 69402
Gm1979
Name: predicted gene 1979
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100038949
Homologene: 69402
4930563M21Rik
Name: RIKEN cDNA 4930563M21 gene
Synonyms: Rfpl3s
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 75258
VEGA: 9
Homologene: 138467
Ighv3-4
Name: immunoglobulin heavy variable V3-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 629833
9630028H03Rik
Name: RIKEN cDNA 9630028H03 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 320684
Trbv12-2
Name: T cell receptor beta, variable 12-2
Synonyms: Tcrb-V5.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 100124674
Gm8979
Name: predicted gene 8979
Synonyms: Gm21884
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 668108
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 125,572,474 bp
  • A to T, chromosome 1 at 173,524,943 bp
  • T to A, chromosome 2 at 85,648,303 bp
  • A to G, chromosome 2 at 92,366,605 bp
  • A to T, chromosome 2 at 135,344,158 bp
  • T to C, chromosome 2 at 180,039,511 bp
  • C to A, chromosome 3 at 87,979,359 bp
  • G to A, chromosome 4 at 135,929,950 bp
  • C to A, chromosome 4 at 155,463,072 bp
  • T to C, chromosome 5 at 26,001,121 bp
  • T to C, chromosome 5 at 27,501,313 bp
  • T to G, chromosome 5 at 74,032,267 bp
  • A to T, chromosome 5 at 91,143,516 bp
  • T to G, chromosome 5 at 109,216,411 bp
  • A to G, chromosome 5 at 134,535,197 bp
  • A to G, chromosome 5 at 138,447,541 bp
  • T to C, chromosome 6 at 29,170,164 bp
  • A to G, chromosome 6 at 41,118,897 bp
  • T to A, chromosome 6 at 140,040,618 bp
  • G to T, chromosome 7 at 41,545,780 bp
  • A to G, chromosome 7 at 43,829,173 bp
  • C to T, chromosome 7 at 46,805,065 bp
  • T to A, chromosome 7 at 51,587,666 bp
  • A to G, chromosome 7 at 63,650,954 bp
  • T to C, chromosome 7 at 103,051,796 bp
  • T to A, chromosome 7 at 106,081,591 bp
  • G to A, chromosome 7 at 120,029,025 bp
  • G to T, chromosome 7 at 139,666,287 bp
  • A to G, chromosome 7 at 140,667,583 bp
  • T to C, chromosome 7 at 141,061,326 bp
  • A to G, chromosome 8 at 13,796,591 bp
  • T to C, chromosome 8 at 110,858,612 bp
  • A to G, chromosome 9 at 6,265,799 bp
  • T to C, chromosome 9 at 55,980,842 bp
  • A to G, chromosome 9 at 65,054,923 bp
  • T to C, chromosome 9 at 75,283,942 bp
  • T to C, chromosome 9 at 107,976,802 bp
  • T to C, chromosome 9 at 109,106,562 bp
  • A to G, chromosome 9 at 111,367,035 bp
  • C to T, chromosome 10 at 77,096,167 bp
  • T to A, chromosome 11 at 21,695,269 bp
  • T to A, chromosome 11 at 21,695,271 bp
  • A to G, chromosome 11 at 98,428,043 bp
  • A to G, chromosome 12 at 4,985,710 bp
  • A to T, chromosome 12 at 114,253,913 bp
  • T to A, chromosome 13 at 27,637,566 bp
  • G to C, chromosome 13 at 32,972,263 bp
  • T to A, chromosome 13 at 43,094,887 bp
  • T to C, chromosome 13 at 97,176,751 bp
  • G to A, chromosome 14 at 103,089,418 bp
  • T to G, chromosome 16 at 21,762,688 bp
  • T to C, chromosome 16 at 44,491,884 bp
  • A to T, chromosome 17 at 46,431,230 bp
  • T to A, chromosome 18 at 65,300,141 bp
  • G to A, chromosome 18 at 78,207,068 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4239 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041056-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.