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Strain Name:
C57BL/6J-MtgxR4332Btlr/Mmmh
Stock Number:
041099-MU
Citation ID:
RRID:MMRRC_041099-MU
Other Names:
R4332 (G1), C57BL/6J-MtgxR4332Btlr
Major Collection:

Strain Information

Chd7
Name: chromodomain helicase DNA binding protein 7
Synonyms: GENA 60, GENA 47, Gena 52, Cyn, A730019I05Rik, WBE1, Whi, Todo, Obt, Mt, Lda, Flo, Edy, Dz, Cycn
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 320790
Homologene: 19067
Slc6a6
Name: solute carrier family 6 (neurotransmitter transporter, taurine), member 6
Synonyms: Taut
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 21366
Homologene: 2291
Tfr2
Name: transferrin receptor 2
Synonyms: Tfr2, Trfr2
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 50765
Homologene: 2428
Usp32
Name: ubiquitin specific peptidase 32
Synonyms: 6430526O11Rik, 2900074J03Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 237898
Homologene: 13066
Fry
Name: FRY microtubule binding protein
Synonyms: cg003, 9330186A19Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 320365
Homologene: 113770
Api5
Name: apoptosis inhibitor 5
Synonyms: AAC-11
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 11800
HGNC: HGNC:594
Homologene: 4809
Urb1
Name: URB1 ribosome biogenesis 1 homolog (S. cerevisiae)
Synonyms: 5730405K23Rik, 4921511H13Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 207932
Homologene: 45941
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 23,375,829 bp
  • T to C, chromosome 1 at 78,469,266 bp
  • G to A, chromosome 1 at 107,369,564 bp
  • G to A, chromosome 1 at 185,455,207 bp
  • G to T, chromosome 1 at 194,807,555 bp
  • G to T, chromosome 2 at 27,215,114 bp
  • A to G, chromosome 2 at 82,977,857 bp
  • G to A, chromosome 2 at 85,024,861 bp
  • A to G, chromosome 2 at 87,107,745 bp
  • A to G, chromosome 2 at 94,422,374 bp
  • C to T, chromosome 2 at 125,740,737 bp
  • C to T, chromosome 3 at 62,484,991 bp
  • C to A, chromosome 3 at 62,604,373 bp
  • C to A, chromosome 3 at 68,695,261 bp
  • A to T, chromosome 3 at 117,322,825 bp
  • A to G, chromosome 3 at 142,725,426 bp
  • G to T, chromosome 4 at 8,854,143 bp
  • G to T, chromosome 4 at 12,145,655 bp
  • A to G, chromosome 4 at 44,895,038 bp
  • G to A, chromosome 4 at 156,338,311 bp
  • T to A, chromosome 5 at 21,661,581 bp
  • A to G, chromosome 5 at 137,571,734 bp
  • C to T, chromosome 5 at 150,381,663 bp
  • G to A, chromosome 6 at 55,968,235 bp
  • G to A, chromosome 6 at 91,723,471 bp
  • A to G, chromosome 6 at 121,657,447 bp
  • A to G, chromosome 6 at 124,842,136 bp
  • T to A, chromosome 7 at 10,052,995 bp
  • T to A, chromosome 7 at 12,844,571 bp
  • A to T, chromosome 7 at 28,437,572 bp
  • G to T, chromosome 7 at 41,216,118 bp
  • T to C, chromosome 7 at 60,155,616 bp
  • A to C, chromosome 7 at 86,363,872 bp
  • T to C, chromosome 7 at 106,972,147 bp
  • T to C, chromosome 8 at 105,709,724 bp
  • C to T, chromosome 9 at 20,822,629 bp
  • G to A, chromosome 10 at 80,188,481 bp
  • T to A, chromosome 10 at 117,002,524 bp
  • T to C, chromosome 10 at 120,364,212 bp
  • C to T, chromosome 11 at 85,103,978 bp
  • T to G, chromosome 11 at 87,567,904 bp
  • A to G, chromosome 11 at 119,436,676 bp
  • T to A, chromosome 12 at 4,712,611 bp
  • A to T, chromosome 12 at 24,252,094 bp
  • A to C, chromosome 12 at 54,062,069 bp
  • T to G, chromosome 16 at 45,686,327 bp
  • T to C, chromosome 16 at 79,034,334 bp
  • A to G, chromosome 16 at 90,774,537 bp
  • T to C, chromosome 17 at 12,962,895 bp
  • T to C, chromosome 18 at 37,265,530 bp
  • A to G, chromosome 18 at 39,775,340 bp
  • A to G, chromosome 19 at 34,234,005 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4332 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041099-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.