Strain Name:
C57BL/6J-MtgxR4396Btlr/Mmmh
Stock Number:
041129-MU
Citation ID:
RRID:MMRRC_041129-MU
Other Names:
R4396 (G1), C57BL/6J-MtgxR4396Btlr
Major Collection:

Gene Information

Elp2
Name: elongator acetyltransferase complex subunit 2
Synonyms: Stat3-interacting protein, StIP1, Statip1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 58523
VEGA: 18
Homologene: 6019
Traip
Name: TRAF-interacting protein
Synonyms: Trip
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 22036
Homologene: 31343
Pdzd2
Name: PDZ domain containing 2
Synonyms: 4930537L06Rik, LOC223364, A930022H17Rik, Pdzk3, Gm21706
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 68070
Homologene: 23393
Sirt1
Name: sirtuin 1
Synonyms: Sir2, Sir2alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 93759
Homologene: 56556
Scn8a
Name: sodium channel, voltage-gated, type VIII, alpha
Synonyms: med, motor end-plate disease, seal, nmf2, ataxia 3, mnd2, mnd-2, nmf58, C630029C19Rik, NaCh6, Nav1.6, NMF335, nmf335, nur14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 20273
Homologene: 7927
Usp32
Name: ubiquitin specific peptidase 32
Synonyms: 6430526O11Rik, 2900074J03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237898
Homologene: 13066
Mtcl1
Name: microtubule crosslinking factor 1
Synonyms: t8219b25, 1110012J17Rik, Soga2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 68617
Homologene: 41017
Sec24a
Name: Sec24 related gene family, member A (S. cerevisiae)
Synonyms: 9430090N21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 77371
Homologene: 70131
Dynll1
Name: dynein light chain LC8-type 1
Synonyms: Pin, 8kDa LC, DLC8, Dnclc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 56455
Homologene: 133063
Dmbt1
Name: deleted in malignant brain tumors 1
Synonyms: CRP-[b], CRP-[a], ebnerin, hensin, vomeroglandin, Crpd, MUCLIN, gp300
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 12945
HGNC: HGNC:2926
Homologene: 68990
Fzd3
Name: frizzled class receptor 3
Synonyms: Fz3, D930050A07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 14365
HGNC: HGNC:4041
Homologene: 23004
Rreb1
Name: ras responsive element binding protein 1
Synonyms: 1110037N09Rik, B930013M22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 68750
Homologene: 2218
Cd200r1
Name: CD200 receptor 1
Synonyms: OX2R, CD200R, Mox2r
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 57781
Homologene: 10957
Fat1
Name: FAT atypical cadherin 1
Synonyms: mFat1, 2310038E12Rik, Fath
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 14107
HGNC: HGNC:3595
Homologene: 66302
Homer3
Name: homer scaffolding protein 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 26558
Homologene: 37972
Zswim1
Name: zinc finger SWIM-type containing 1
Synonyms: 2410003H12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 71971
Homologene: 12429
Gemin5
Name: gem nuclear organelle associated protein 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216766
Homologene: 9155
Piezo1
Name: piezo-type mechanosensitive ion channel component 1
Synonyms: Piezo1, Fam38a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234839
Homologene: 124356
Plxna2
Name: plexin A2
Synonyms: OCT, Plxn2, 2810428A13Rik, PlexA2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 18845
HGNC: HGNC:9100
Homologene: 56427
Treml2
Name: triggering receptor expressed on myeloid cells-like 2
Synonyms: LOC328833
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 328833
VEGA: 17
Homologene: 81906
Trpv5
Name: transient receptor potential cation channel, subfamily V, member 5
Synonyms: CaT2, ECaC1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 194352
HGNC: HGNC:3145
Homologene: 10520
Scnn1g
Name: sodium channel, nonvoltage-gated 1 gamma
Synonyms: ENaC gamma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 20278
Homologene: 20280
Tns2
Name: tensin 2
Synonyms: nep, nph, Tenc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 209039
VEGA: 15
Homologene: 37077
Col12a1
Name: collagen, type XII, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 12816
HGNC: HGNC:2188
Homologene: 3217
2410017I17Rik
Name: RIKEN cDNA 2410017I17 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 675325
Or10aa3
Name: olfactory receptor family 10 subfamily AA member 3
Synonyms: GA_x6K02T2P20D-21124681-21123743, MOR123-2, Olfr432
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 258711
Homologene: 81532
Col19a1
Name: collagen, type XIX, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12823
HGNC: HGNC:2196
Homologene: 55608
Cdhr2
Name: cadherin-related family member 2
Synonyms: LOC268663, Pcdh24
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 268663
Homologene: 134510
Actn2
Name: actinin alpha 2
Synonyms: 1110008F24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 11472
HGNC: HGNC:164
Homologene: 31016
Mfsd13a
Name: major facilitator superfamily domain containing 13a
Synonyms: 4930449A08Rik, 4930538D17Rik, Tmem180
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 75146
Homologene: 12592
Slc15a2
Name: solute carrier family 15 (H+/peptide transporter), member 2
Synonyms: Pept2, 8430408C16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 57738
Homologene: 56912
Vmn2r79
Name: vomeronasal 2, receptor 79
Synonyms: EG621430
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 621430
Homologene: 115466
Mtus2
Name: microtubule associated tumor suppressor candidate 2
Synonyms: 5730592G18Rik, A730013O20Rik, C130038G02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 77521
Homologene: 78141
Or4f57
Name: olfactory receptor family 4 subfamily F member 57
Synonyms: GA_x6K02T2Q125-73008844-73007882, MOR245-22, Olfr1308
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 258258
Homologene: 45797
Wdr90
Name: WD repeat domain 90
Synonyms: 3230401M21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 106618
Homologene: 27066
Plxnb1
Name: plexin B1
Synonyms: 2900002G15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235611
HGNC: HGNC:9103
Homologene: 130508
Mylk
Name: myosin, light polypeptide kinase
Synonyms: telokin, Mlck, MLCK210, MLCK108, 9530072E15Rik, A930019C19Rik, nmMlck
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 107589
HGNC: HGNC:7590
Homologene: 14202
Mdga1
Name: MAM domain containing glycosylphosphatidylinositol anchor 1
Synonyms: 1200011I03Rik, Mamdc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 74762
Homologene: 17780
Llgl1
Name: LLGL1 scribble cell polarity complex component
Synonyms: Lgl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 16897
HGNC: HGNC:6628
Homologene: 31220
Mpp4
Name: membrane protein, palmitoylated 4 (MAGUK p55 subfamily member 4)
Synonyms: DLG6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227157
Homologene: 23296
Acsm2
Name: acyl-CoA synthetase medium-chain family member 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233799
Homologene: 70404
Galnt7
Name: polypeptide N-acetylgalactosaminyltransferase 7
Synonyms: ppGaNTase-T7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 108150
HGNC: HGNC:4129
Homologene: 9685
Traf5
Name: TNF receptor-associated factor 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 22033
Homologene: 27079
Btbd7
Name: BTB (POZ) domain containing 7
Synonyms: FUP1, 5730507E09Rik, E130118E17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 238386
VEGA: 12
Homologene: 34300
Kcnj4
Name: potassium inwardly-rectifying channel, subfamily J, member 4
Synonyms: IRK3, Kir 2.3, MB-IRK3, Kcnf2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 16520
VEGA: 15
HGNC: HGNC:6265
Homologene: 3653
Rpl7-ps8
Name: ribosomal protein L7, pseudogene 8
Synonyms: Gm5045
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 268809
VEGA: 15
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 24,510,866 bp
  • T to C, chromosome 1 at 59,144,802 bp
  • A to G, chromosome 1 at 174,050,446 bp
  • A to T, chromosome 1 at 191,997,845 bp
  • C to T, chromosome 1 at 194,749,317 bp
  • A to T, chromosome 2 at 87,508,530 bp
  • A to G, chromosome 2 at 111,960,215 bp
  • A to G, chromosome 2 at 164,825,449 bp
  • A to T, chromosome 5 at 115,300,641 bp
  • T to A, chromosome 5 at 148,203,938 bp
  • T to C, chromosome 6 at 41,657,896 bp
  • A to G, chromosome 7 at 87,001,891 bp
  • T to C, chromosome 7 at 119,595,920 bp
  • T to G, chromosome 7 at 121,740,427 bp
  • T to C, chromosome 7 at 131,116,632 bp
  • T to A, chromosome 8 at 44,952,346 bp
  • A to T, chromosome 8 at 57,538,181 bp
  • G to A, chromosome 8 at 70,290,143 bp
  • G to T, chromosome 8 at 122,498,674 bp
  • G to A, chromosome 9 at 79,697,576 bp
  • C to G, chromosome 9 at 107,959,487 bp
  • T to C, chromosome 9 at 109,100,223 bp
  • T to C, chromosome 10 at 63,321,998 bp
  • A to G, chromosome 11 at 51,715,164 bp
  • T to A, chromosome 11 at 58,139,549 bp
  • T to C, chromosome 11 at 60,706,008 bp
  • G to A, chromosome 11 at 85,053,975 bp
  • A to G, chromosome 12 at 102,785,293 bp
  • A to G, chromosome 13 at 12,310,879 bp
  • C to T, chromosome 13 at 37,930,443 bp
  • A to G, chromosome 13 at 54,715,665 bp
  • A to G, chromosome 14 at 65,235,605 bp
  • A to T, chromosome 15 at 12,387,646 bp
  • T to C, chromosome 15 at 59,211,613 bp
  • C to A, chromosome 15 at 79,484,673 bp
  • A to G, chromosome 15 at 100,972,830 bp
  • C to T, chromosome 15 at 102,108,934 bp
  • C to T, chromosome 16 at 34,912,275 bp
  • A to G, chromosome 16 at 36,772,447 bp
  • T to A, chromosome 16 at 44,766,054 bp
  • A to T, chromosome 17 at 25,853,849 bp
  • T to C, chromosome 17 at 29,850,517 bp
  • C to A, chromosome 17 at 36,161,969 bp
  • T to C, chromosome 17 at 48,308,114 bp
  • T to C, chromosome 17 at 66,344,225 bp
  • T to A, chromosome 18 at 24,609,650 bp
  • C to T, chromosome 19 at 46,371,992 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4396 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041129-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.