Strain Name:
C57BL/6J-MtgxR4453Btlr/Mmmh
Stock Number:
041152-MU
Citation ID:
RRID:MMRRC_041152-MU
Other Names:
R4453 (G1), C57BL/6J-MtgxR4453Btlr
Major Collection:

Gene Information

Prkd3
Name: protein kinase D3
Synonyms: 5730497N19Rik, PKD3, 4930557O20Rik, Prkcn
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 75292
HGNC: HGNC:9408
Homologene: 2055
Ppp1r1c
Name: protein phosphatase 1, regulatory inhibitor subunit 1C
Synonyms: 4930565M23Rik, 4930579P15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 75276
Homologene: 18170
Ckap5
Name: cytoskeleton associated protein 5
Synonyms: D730027C18Rik, 3110043H24Rik, 4930432B04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 75786
Homologene: 8844
Helz
Name: helicase with zinc finger domain
Synonyms: 3110078M01Rik, 9430093I07Rik, 9630002H22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 78455
Homologene: 8918
Taf12
Name: TATA-box binding protein associated factor 12
Synonyms: 20kDa, 2810422D08Rik, Taf2J
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 66464
Homologene: 68477
Zzef1
Name: zinc finger, ZZ-type with EF hand domain 1
Synonyms: 8430405D05Rik, C130099L13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 195018
Homologene: 9027
Rims2
Name: regulating synaptic membrane exocytosis 2
Synonyms: 2810036I15Rik, RIM2, Syt3-rs
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 116838
VEGA: 15
Homologene: 81639
Hus1
Name: HUS1 checkpoint clamp component
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 15574
HGNC: HGNC:5309
Homologene: 37932
Dmbt1
Name: deleted in malignant brain tumors 1
Synonyms: gp300, Crpd, hensin, ebnerin, CRP-[a], vomeroglandin, CRP-[b], MUCLIN
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 12945
HGNC: HGNC:2926
Homologene: 68990
Dnajc10
Name: DnaJ heat shock protein family (Hsp40) member C10
Synonyms: JPDI, D2Ertd706e, 1200006L06Rik, ERdj5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 66861
Homologene: 10358
Prune2
Name: prune homolog 2
Synonyms: 6330414G02Rik, A230083H22Rik, A330102H22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 353211
VEGA: 19
Homologene: 18939
Cpne6
Name: copine VI
Synonyms: neuronal copine
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
Alteration at locus: Chemically Induced
NCBI: 12891
HGNC: HGNC:2319
Homologene: 81815
Limd1
Name: LIM domains containing 1
Synonyms: D9Ertd192e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 29806
VEGA: 9
HGNC: HGNC:6612
Homologene: 8478
Wwtr1
Name: WW domain containing transcription regulator 1
Synonyms: 2610021I22Rik, transcriptional coactivator with PDZ binding motif, 2310058J06Rik, TAZ
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 97064
Homologene: 9159
Zfp473
Name: zinc finger protein 473
Synonyms: D030014N22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 243963
Homologene: 18698
Grm2
Name: glutamate receptor, metabotropic 2
Synonyms: 4930441L02Rik, mGluR2, Gprc1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 108068
HGNC: HGNC:4594
Homologene: 20229
Hspa1a
Name: heat shock protein 1A
Synonyms: Hsp70-3, Hsp70.3, Hsp68, Hsp70a1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 193740
Homologene: 74294
Atp10a
Name: ATPase, class V, type 10A
Synonyms: pfatp, Atp10c
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 11982
Homologene: 56461
Fat3
Name: FAT atypical cadherin 3
Synonyms: LOC382129, 9430076A06Rik, LOC234973, D430038H04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 270120
VEGA: 9
Homologene: 82252
Stard9
Name: START domain containing 9
Synonyms: E230025N21Rik, 4831403C07Rik, Kif16a, N-3 kinesin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 668880
Homologene: 130712
Lipe
Name: lipase, hormone sensitive
Synonyms: HSL, 4933403G17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 16890
HGNC: HGNC:6621
Homologene: 3912
Tma16
Name: translation machinery associated 16
Synonyms: 1810029B16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 66282
Homologene: 10146
Ces2h
Name: carboxylesterase 2H
Synonyms: Gm5744
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 436059
HGNC: HGNC:1864
Homologene: 128645
Kcnh1
Name: potassium voltage-gated channel, subfamily H (eag-related), member 1
Synonyms: ether a go-go, Kv10.1, Eag1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 16510
HGNC: HGNC:6250
Homologene: 68242
Zfp286
Name: zinc finger protein 286
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 192651
Homologene: 56892
Olfr814
Name: olfactory receptor 814
Synonyms: MOR113-8, MOR113-5, GA_x6K02T2PULF-11553313-11552381
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 259165
Homologene: 133585
Ano7
Name: anoctamin 7
Synonyms: NGEP, Tmem16g, Pcanap5, NGEP-L, IPCA-5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 404545
Homologene: 45787
Prdm13
Name: PR domain containing 13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 230025
Homologene: 11000
Gusb
Name: glucuronidase, beta
Synonyms: asd, Gus, Gus-s, Gus-u, adipose storage deficiency, Gut, Gus-r, Gus-t, Gur, g
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 110006
HGNC: HGNC:4696
Homologene: 37271
Ntpcr
Name: nucleoside-triphosphatase, cancer-related
Synonyms: 2310079N02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 66566
Homologene: 11989
Tssk5
Name: testis-specific serine kinase 5
Synonyms: 1700091F14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 73542
VEGA: 15
Homologene: 18832
Gm10913
Name: predicted pseudogene 10913
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 100503125
Homologene: 136501
Ccdc86
Name: coiled-coil domain containing 86
Synonyms: 6720480F16Rik, cyclon, 4933411H20Rik, D19Ertd678e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 108673
Homologene: 41531
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 93,394,353 bp
  • C to A, chromosome 1 at 192,505,517 bp
  • A to G, chromosome 2 at 79,708,231 bp
  • T to C, chromosome 2 at 80,346,623 bp
  • T to A, chromosome 2 at 91,548,845 bp
  • A to G, chromosome 2 at 120,697,791 bp
  • T to C, chromosome 3 at 57,575,259 bp
  • G to T, chromosome 4 at 21,679,464 bp
  • A to G, chromosome 4 at 132,282,995 bp
  • A to G, chromosome 5 at 129,998,483 bp
  • T to A, chromosome 7 at 25,397,690 bp
  • T to A, chromosome 7 at 44,733,254 bp
  • TGGCGGCGGC to TGGCGGC, chromosome 7 at 58,658,500 bp
  • T to C, chromosome 7 at 131,040,934 bp
  • C to T, chromosome 8 at 66,484,171 bp
  • G to A, chromosome 8 at 105,014,656 bp
  • G to A, chromosome 8 at 125,736,190 bp
  • G to A, chromosome 9 at 15,998,271 bp
  • G to A, chromosome 9 at 106,653,879 bp
  • C to A, chromosome 9 at 123,480,294 bp
  • T to C, chromosome 10 at 129,874,661 bp
  • A to T, chromosome 11 at 9,006,035 bp
  • C to G, chromosome 11 at 62,780,204 bp
  • T to C, chromosome 11 at 72,872,639 bp
  • C to T, chromosome 11 at 107,672,629 bp
  • G to A, chromosome 14 at 55,512,597 bp
  • T to A, chromosome 15 at 39,292,208 bp
  • G to A, chromosome 15 at 76,374,543 bp
  • A to G, chromosome 16 at 36,209,620 bp
  • A to G, chromosome 17 at 34,970,293 bp
  • C to A, chromosome 17 at 78,983,546 bp
  • G to T, chromosome 19 at 10,948,519 bp
  • T to C, chromosome 19 at 17,121,910 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4453 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041152-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.