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Availability & Fees Order this Strain
This line harbors a frameshifting C duplication homologous to a reported case of human incontinentia pigmenti ("XL344-04", NM_001099857.3:c.1167dup, PMID:11179023). The mouse model reproduces the associated truncating mutation and adds, 5' in-frame, an EGFP open-reading frame (underlined below). Expression of EGFP has been confirmed in embryonic fibroblasts.
HGVS nomenclature, C insertion in the mouse model:
Homozygous: lethal at embryonic stage E12.5
Heterozygous: skin lesion and eye lesion, developmental delay
Pasparakis M, Alexopoulou L, Episkopou V, Kollias G. Immune and inflammatory responses in TNF alpha-deficient mice: a critical requirement for TNF alpha in the formation of primary B cell follicles, follicular dendritic cell networks and germinal centers, and in the maturation of the humoral immune response. J Exp Med. 1996 Oct 1;184(4):1397-411. (Medline PMID: 8879212)
Aradhya S, Courtois G, Rajkovic A, Lewis RA, Levy M, Israël A, Nelson DL.Atypical forms of incontinentia pigmenti in male individuals result frommutations of a cytosine tract in exon 10 of NEMO (IKK-gamma). Am J Hum Genet.2001 Mar;68(3):765-71. Epub 2001 Feb 8. (Medline PMID: 11179023)
The donor maintained these mice on the B6J.129S7-Tnftm1Gkl/J background to improve survival rates and minimize skin lesions.
Colony Surveillance Program and Current Health Reports
Wild-type (C57BL/6) female x Heterozygous male or reciprocal mating
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
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