Strain Name:
C57BL/6J-MtgxR4492Btlr/Mmmh
Stock Number:
041581-MU
Citation ID:
RRID:MMRRC_041581-MU
Other Names:
R4492 (G1), C57BL/6J-MtgxR4492Btlr
Major Collection:

Gene Information

Slc12a5
Name: solute carrier family 12, member 5
Synonyms: KCC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 57138
Homologene: 10665
Nos2
Name: nitric oxide synthase 2, inducible
Synonyms: Nos-2, Nos2a, NOS-II, iNOS
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18126
HGNC: HGNC:7873
Homologene: 55473
Zfp930
Name: zinc finger protein 930
Synonyms: D10627, zinc finger protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234358
Pdzd2
Name: PDZ domain containing 2
Synonyms: A930022H17Rik, 4930537L06Rik, Gm21706, LOC223364, Pdzk3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 68070
Homologene: 23393
Vps54
Name: VPS54 GARP complex subunit
Synonyms: wr, Vps54l, mSLP8, 5330404P15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 245944
Homologene: 5605
Prex2
Name: phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2
Synonyms: 6230420N16Rik, C030045D06Rik, Depdc2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 109294
Homologene: 23523
Rock2
Name: Rho-associated coiled-coil containing protein kinase 2
Synonyms: Rock-II, ROKalpha, Rho-kinase, Rock2m, B230113H15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 19878
VEGA: 12
Homologene: 21010
Etl4
Name: enhancer trap locus 4
Synonyms: 6620402G01Rik, E330027G05Rik, Etl-4, 9430077C05Rik, Skt, Sickle tail
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 208618
Homologene: 10477
Btbd9
Name: BTB (POZ) domain containing 9
Synonyms: 1700023F20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224671
VEGA: 17
Homologene: 14995
Cog6
Name: component of oligomeric golgi complex 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 67542
Homologene: 10802
Dmtf1
Name: cyclin D binding myb-like transcription factor 1
Synonyms: Dmp1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 23857
Homologene: 8017
Rsf1
Name: remodeling and spacing factor 1
Synonyms: 4832420A03Rik, C030033M12Rik, p325, XAP8, Hbxap
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233532
Homologene: 41142
Focad
Name: focadhesin
Synonyms: BC057079
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230393
Homologene: 9842
Ank3
Name: ankyrin 3, epithelial
Synonyms: AnkG, Ank-3, Ankyrin-3, 2900054D09Rik, Ankyrin-G
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 11735
HGNC: HGNC:494
Homologene: 56908
Mc4r
Name: melanocortin 4 receptor
Synonyms: Pkcp, Fatboy
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 17202
VEGA: 18
HGNC: HGNC:6932
Homologene: 4320
Nup37
Name: nucleoporin 37
Synonyms: 2810039M17Rik, 2410003L22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 69736
VEGA: 10
Homologene: 11441
Clpb
Name: ClpB caseinolytic peptidase B
Synonyms: Skd3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 20480
Homologene: 32067
Amph
Name: amphiphysin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218038
HGNC: HGNC:471
Homologene: 121585
Vps4b
Name: vacuolar protein sorting 4B
Synonyms: Skd1, 8030489C12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 20479
Homologene: 37976
Anapc16
Name: anaphase promoting complex subunit 16
Synonyms: 2310005G07Rik, D10Ertd641e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 52717
Homologene: 11957
Zfp236
Name: zinc finger protein 236
Synonyms: LOC240456
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 329002
Homologene: 7198
Dusp4
Name: dual specificity phosphatase 4
Synonyms: E130306H24Rik, 2700078F24Rik, MKP2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 319520
HGNC: HGNC:3070
Homologene: 1065
Edc4
Name: enhancer of mRNA decapping 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234699
Homologene: 40937
H2-T23
Name: histocompatibility 2, T region locus 23
Synonyms: H-2T23, Qa-1, Qa1, T18c(37), T18c, 37c, 37b, T23d, T23b, Qed-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 15040
HGNC: HGNC:4962
Homologene: 134018
Kcna4
Name: potassium voltage-gated channel, shaker-related subfamily, member 4
Synonyms: Kv1.4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16492
HGNC: HGNC:6222
Homologene: 20514
Idh2
Name: isocitrate dehydrogenase 2 (NADP+), mitochondrial
Synonyms: Idh-2, IDPm
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 269951
HGNC: HGNC:5383
Homologene: 37590
Rasef
Name: RAS and EF hand domain containing
Synonyms: RAB45
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242505
Homologene: 28424
Mroh8
Name: maestro heat-like repeat family member 8
Synonyms: 4922505G16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 629499
Homologene: 51864
Olfr273
Name: olfactory receptor 273
Synonyms: MOR262-8, GA_x6K02T2N78B-7137430-7138383
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 258821
Homologene: 73999
Ttc21a
Name: tetratricopeptide repeat domain 21A
Synonyms: Thm2, 4921538N17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 74052
Homologene: 14728
Tmem30a
Name: transmembrane protein 30A
Synonyms: 2010200I23Rik, Cdc50a, D9Wsu20e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 69981
Homologene: 110703
Top2b
Name: topoisomerase (DNA) II beta
Synonyms: Top-2, D230016L12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 21974
Homologene: 134711
Srsf9
Name: serine and arginine-rich splicing factor 9
Synonyms: SRp30c, Sfrs9, 2610029M16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 108014
Homologene: 20819
Chil3
Name: chitinase-like 3
Synonyms: Chi3l3, Ym1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 12655
Homologene: 74931
Actr6
Name: ARP6 actin-related protein 6
Synonyms: CDA12, Arp6, ArpX, 2010200J04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 67019
VEGA: 10
Homologene: 6451
Cyp2d22
Name: cytochrome P450, family 2, subfamily d, polypeptide 22
Synonyms: 2D22
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 56448
VEGA: 15
Homologene: 75003
Serpina6
Name: serine (or cysteine) peptidase inhibitor, clade A, member 6
Synonyms: Cbg
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 12401
HGNC: HGNC:1540
Homologene: 20417
Olfr231
Name: olfactory receptor 231
Synonyms: Olfr244, GA_x6K02T2P20D-21053942-21054499, MOR105-4, GA_x6K02T2LER7-6-623, GA_x6K02T02K39-452-3, Olfr425
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 404222
Homologene: 17363
Prss8
Name: protease, serine 8 (prostasin)
Synonyms: mCAP1, CAP1, 2410039E18Rik, fr
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 76560
HGNC: HGNC:9491
Homologene: 20613
Lum
Name: lumican
Synonyms: SLRR2D, Ldc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 17022
VEGA: 10
HGNC: HGNC:6724
Homologene: 37614
Dhrs7
Name: dehydrogenase/reductase (SDR family) member 7
Synonyms: 2310016E22Rik, 5730564L20Rik, retDSR4, retSDR4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 66375
VEGA: 12
Homologene: 9350
Irgm1
Name: immunity-related GTPase family M member 1
Synonyms: LRG-47, Irgm, Ifi1, Iigp3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 15944
Homologene: 134089
Jph1
Name: junctophilin 1
Synonyms: ENSMUSG00000054314, JP-1, mitsugumin72
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 57339
Homologene: 10761
Gpr156
Name: G protein-coupled receptor 156
Synonyms: Gababl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 239845
VEGA: 16
Homologene: 17683
Kcna1
Name: potassium voltage-gated channel, shaker-related subfamily, member 1
Synonyms: mceph, Kv1.1, Shak, mouse brain potassium channel protein-1, MBK1, Mk-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 16485
HGNC: HGNC:6218
Homologene: 183
Fdxacb1
Name: ferredoxin-fold anticodon binding domain containing 1
Synonyms: D630004A14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 382137
Homologene: 19813
Gm11787
Name: predicted gene 11787
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 666513
Pla1a
Name: phospholipase A1 member A
Synonyms: Ps-pla1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 85031
VEGA: 16
Homologene: 9294
Taf1
Name: TATA-box binding protein associated factor 1
Synonyms: B430306D02Rik, Taf2a, Ccg1, KAT4, Ccg-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 270627
Homologene: 37942
Fam174a
Name: family with sequence similarity 174, member A
Synonyms: 2310044D20Rik, Tmem157
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 67698
Homologene: 12175
Zfp612
Name: zinc finger protein 612
Synonyms: B230354B21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234725
Homologene: 27083
Gm11193
Name: predicted gene 11193
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Pitx1
Name: paired-like homeodomain transcription factor 1
Synonyms: Bft, Potx, P-OTX, Ptx1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 18740
HGNC: HGNC:9004
Homologene: 20584
Gpr17
Name: G protein-coupled receptor 17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 574402
HGNC: HGNC:4471
Homologene: 83191
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 11,162,263 bp
  • A to C, chromosome 1 at 16,997,546 bp
  • T to C, chromosome 1 at 95,313,976 bp
  • C to A, chromosome 1 at 106,786,755 bp
  • C to T, chromosome 1 at 174,117,204 bp
  • G to A, chromosome 2 at 20,806,865 bp
  • G to A, chromosome 2 at 107,296,091 bp
  • A to T, chromosome 2 at 157,258,040 bp
  • T to C, chromosome 2 at 164,979,343 bp
  • C to T, chromosome 3 at 52,992,842 bp
  • A to G, chromosome 3 at 106,155,701 bp
  • A to T, chromosome 4 at 3,506,105 bp
  • T to C, chromosome 4 at 52,855,764 bp
  • A to C, chromosome 4 at 73,734,503 bp
  • G to A, chromosome 4 at 88,359,905 bp
  • A to G, chromosome 5 at 9,140,379 bp
  • T to A, chromosome 5 at 115,332,592 bp
  • C to T, chromosome 6 at 126,642,275 bp
  • GGGCCAGG to GGG, chromosome 7 at 80,098,342 bp
  • CGGCGGCGG to CGGCGGCGGGGGCGGCGG, chromosome 7 at 97,579,923 bp
  • T to C, chromosome 7 at 101,787,722 bp
  • A to G, chromosome 7 at 127,929,807 bp
  • C to T, chromosome 8 at 34,807,736 bp
  • C to T, chromosome 8 at 69,228,246 bp
  • GGATTTTAGCCA to G, chromosome 8 at 105,885,068 bp
  • C to A, chromosome 8 at 110,089,297 bp
  • T to C, chromosome 9 at 50,770,247 bp
  • T to C, chromosome 9 at 79,777,285 bp
  • T to C, chromosome 9 at 119,941,280 bp
  • A to G, chromosome 10 at 59,990,902 bp
  • T to A, chromosome 10 at 69,808,925 bp
  • T to A, chromosome 10 at 88,174,929 bp
  • T to G, chromosome 10 at 89,725,814 bp
  • C to A, chromosome 10 at 97,568,438 bp
  • A to G, chromosome 11 at 21,316,875 bp
  • G to A, chromosome 11 at 48,866,128 bp
  • C to T, chromosome 11 at 78,292,931 bp
  • A to G, chromosome 11 at 78,950,095 bp
  • T to A, chromosome 12 at 16,977,683 bp
  • A to T, chromosome 12 at 72,653,125 bp
  • A to G, chromosome 12 at 103,646,887 bp
  • T to C, chromosome 13 at 19,149,758 bp
  • T to C, chromosome 13 at 55,828,652 bp
  • T to G, chromosome 14 at 16,409,189 bp
  • C to T, chromosome 15 at 12,385,637 bp
  • A to C, chromosome 15 at 12,419,481 bp
  • G to A, chromosome 15 at 82,374,370 bp
  • T to A, chromosome 16 at 37,992,106 bp
  • C to T, chromosome 16 at 38,409,610 bp
  • A to G, chromosome 17 at 30,527,571 bp
  • A to T, chromosome 17 at 36,032,166 bp
  • A to T, chromosome 18 at 31,947,251 bp
  • A to G, chromosome 18 at 66,859,640 bp
  • A to T, chromosome 18 at 82,630,000 bp
  • G to T, chromosome X at 101,543,059 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4492 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041581-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.