Strain Name:
C57BL/6J-MtgxR4554Btlr/Mmmh
Stock Number:
041596-MU
Citation ID:
RRID:MMRRC_041596-MU
Other Names:
R4554 (G1), C57BL/6J-MtgxR4554Btlr
Major Collection:

Gene Information

Psmd13
Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 13
Synonyms: S11, 26S proteasome subunit p40.5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 23997
HGNC: HGNC:9558
Homologene: 2110
Phf20l1
Name: PHD finger protein 20-like 1
Synonyms: CGI-72, E130113K22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 239510
VEGA: 15
Homologene: 9341
Ubr1
Name: ubiquitin protein ligase E3 component n-recognin 1
Synonyms: E3 alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 22222
Homologene: 7582
Tex2
Name: testis expressed gene 2
Synonyms: Taz4, Def-5, 4930568E07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 21763
Homologene: 32414
Ep300
Name: E1A binding protein p300
Synonyms: KAT3B, p300
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 328572
HGNC: HGNC:3373
Homologene: 1094
Marf1
Name: meiosis regulator and mRNA stability 1
Synonyms: 4921513D23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 223989
VEGA: 16
Homologene: 40967
Alms1
Name: ALMS1, centrosome and basal body associated
Synonyms: Alstrom syndrome 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 236266
HGNC: HGNC:428
Homologene: 49406
Ap1b1
Name: adaptor protein complex AP-1, beta 1 subunit
Synonyms: beta-prime adaptin, Adtb1, b2b1660Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 11764
HGNC: HGNC:554
Homologene: 21972
Poc5
Name: POC5 centriolar protein
Synonyms: 1200014M14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 67463
VEGA: 13
Homologene: 12141
Ppp1r12b
Name: protein phosphatase 1, regulatory subunit 12B
Synonyms: 9530009M10Rik, 1810037O03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 329251
HGNC: HGNC:7619
Homologene: 135710
Ryr1
Name: ryanodine receptor 1, skeletal muscle
Synonyms: skrr, Ryr, calcium release channel isoform 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 20190
Homologene: 68069
Pitpnm1
Name: phosphatidylinositol transfer protein, membrane-associated 1
Synonyms: RdgB, DRES9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 18739
HGNC: HGNC:9003
Homologene: 3608
Rfx8
Name: regulatory factor X 8
Synonyms: 4933400N17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 619289
Homologene: 82436
Abca4
Name: ATP-binding cassette, sub-family A (ABC1), member 4
Synonyms: RmP, Rim protein, D430003I15Rik, Abc10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 11304
HGNC: HGNC:34
Homologene: 298
Ahnak
Name: AHNAK nucleoprotein (desmoyokin)
Synonyms: 1110004P15Rik, 2310047C17Rik, DY6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 66395
VEGA: 19
HGNC: HGNC:347
Homologene: 67425
Cap2
Name: CAP, adenylate cyclase-associated protein, 2 (yeast)
Synonyms: 2810452G09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 67252
Homologene: 101397
Tmc5
Name: transmembrane channel-like gene family 5
Synonyms: 4932443L08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 74424
Homologene: 11713
Ngrn
Name: neugrin, neurite outgrowth associated
Synonyms: neurite outgrowth associated protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 83485
Homologene: 32330
Thap12
Name: THAP domain containing 12
Synonyms: 2900052B10Rik, Prkrir, Dap4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 72981
HGNC: HGNC:9440
Homologene: 37952
Ccdc7b
Name: coiled-coil domain containing 7B
Synonyms: 1700008F21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 75453
Homologene: 49919
Rtl1
Name: retrotransposon Gaglike 1
Synonyms: Mart1, Mor1, Mar
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 353326
VEGA: 12
Homologene: 120261
Adamts17
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 17
Synonyms: AU023434
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 233332
Homologene: 16373
Mfsd11
Name: major facilitator superfamily domain containing 11
Synonyms: 2600014M03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 69900
Homologene: 11517
Olfr1084
Name: olfactory receptor 1084
Synonyms: MOR192-3, GA_x6K02T2Q125-48121788-48120847, MOR192-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258235
HGNC: null
Homologene: 104285
Amh
Name: anti-Mullerian hormone
Synonyms: MIS, Mullerian inhibiting substance
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 11705
VEGA: 10
HGNC: HGNC:464
Homologene: 68060
Zswim9
Name: zinc finger SWIM-type containing 9
Synonyms: 6330408A02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 321008
Homologene: 52386
Lsamp
Name: limbic system-associated membrane protein
Synonyms: D930023J12Rik, B130007O04Rik, Lamp, Lam
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 268890
HGNC: HGNC:6705
Homologene: 20533
Rhbdd3
Name: rhomboid domain containing 3
Synonyms: 5730411O18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 279766
HGNC: HGNC:1308
Homologene: 8175
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 39,680,940 bp
  • C to T, chromosome 1 at 134,760,392 bp
  • T to A, chromosome 2 at 86,638,779 bp
  • T to C, chromosome 2 at 120,955,700 bp
  • C to T, chromosome 3 at 122,156,343 bp
  • G to A, chromosome 6 at 85,624,617 bp
  • T to C, chromosome 7 at 13,277,162 bp
  • T to C, chromosome 7 at 29,105,008 bp
  • A to T, chromosome 7 at 67,027,893 bp
  • C to T, chromosome 7 at 80,264,701 bp
  • A to G, chromosome 7 at 98,715,845 bp
  • T to C, chromosome 7 at 118,670,733 bp
  • T to C, chromosome 7 at 140,887,120 bp
  • T to C, chromosome 8 at 129,183,625 bp
  • A to T, chromosome 10 at 80,807,051 bp
  • A to G, chromosome 11 at 5,009,547 bp
  • C to T, chromosome 11 at 5,105,946 bp
  • G to T, chromosome 11 at 106,544,386 bp
  • T to G, chromosome 11 at 116,861,580 bp
  • T to C, chromosome 12 at 109,594,328 bp
  • T to A, chromosome 13 at 46,635,774 bp
  • A to G, chromosome 13 at 96,403,021 bp
  • A to G, chromosome 15 at 66,597,367 bp
  • T to A, chromosome 15 at 81,601,430 bp
  • T to C, chromosome 16 at 14,153,977 bp
  • G to C, chromosome 16 at 42,144,075 bp
  • A to G, chromosome 19 at 4,103,085 bp
  • G to A, chromosome 19 at 9,014,930 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4554 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041596-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.