Strain Name:
C57BL/6J-MtgxR4727Btlr/Mmmh
Stock Number:
041602-MU
Citation ID:
RRID:MMRRC_041602-MU
Other Names:
R4727 (G1), C57BL/6J-MtgxR4727Btlr
Major Collection:

Gene Information

Kcnj10
Name: potassium inwardly-rectifying channel, subfamily J, member 10
Synonyms: Kir1.2, BIR10, BIRK-1, Kir4.1, Kir4.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 16513
HGNC: HGNC:6256
Homologene: 1689
Satb1
Name: special AT-rich sequence binding protein 1
Synonyms: 2610306G12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 20230
Homologene: 2232
Slc17a7
Name: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7
Synonyms: 2900052E22Rik, Vglut1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 72961
Homologene: 113454
B4galt1
Name: UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1
Synonyms: beta-1,4-GalT1, beta 1,4-Galactosyltransferase I, GalT, B-1,4-GalT1, Ggtb2, b1,4-Galactosyltransferase I, Ggtb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 14595
HGNC: HGNC:924
Homologene: 20378
Ash2l
Name: ASH2 like histone lysine methyltransferase complex subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 23808
HGNC: HGNC:744
Homologene: 3436
Ghitm
Name: growth hormone inducible transmembrane protein
Synonyms: 1010001P14Rik, C77840, Tmbim5, PTD010
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 66092
VEGA: 14
Homologene: 8667
Rps6kb1
Name: ribosomal protein S6 kinase, polypeptide 1
Synonyms: S6K1, p70s6k, p70/85s6k, 2610318I15Rik, p70S6K1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 72508
Homologene: 81703
Wdfy3
Name: WD repeat and FYVE domain containing 3
Synonyms: Bwf1, 2610509D04Rik, Bchs, Ggtb3, Alfy, D5Ertd66e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 72145
Homologene: 22855
Rangap1
Name: RAN GTPase activating protein 1
Synonyms: Fug1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 19387
VEGA: 15
HGNC: HGNC:9854
Homologene: 55700
Cep290
Name: centrosomal protein 290
Synonyms: b2b1752Clo, b2b1454Clo, Kiaa, Nphp6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216274
VEGA: 10
Homologene: 77213
Plk4
Name: polo like kinase 4
Synonyms: Stk18, Sak
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 20873
Homologene: 7962
Faf1
Name: Fas-associated factor 1
Synonyms: Fam, Dffrx
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 14084
HGNC: HGNC:3578
Homologene: 5120
Bnip3
Name: BCL2/adenovirus E1B interacting protein 3
Synonyms: Nip3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 12176
HGNC: HGNC:1084
Homologene: 2990
Wdr33
Name: WD repeat domain 33
Synonyms: 2310011G05Rik, WDC146, 2810021O11Rik, 8430413N20Rik, 1110001N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 74320
VEGA: 18
Homologene: 56807
Esr1
Name: estrogen receptor 1 (alpha)
Synonyms: ERa, Estr, ESR, ERalpha, ER-alpha, Nr3a1, ER[a], Estra
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 13982
HGNC: HGNC:3467
Homologene: 47906
Tank
Name: TRAF family member-associated Nf-kappa B activator
Synonyms: I-TRAF, E430026L09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 21353
Homologene: 3081
Nfrkb
Name: nuclear factor related to kappa B binding protein
Synonyms: A530090G11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235134
HGNC: HGNC:7802
Homologene: 4492
Tefm
Name: transcription elongation factor, mitochondrial
Synonyms: FLJ22729, 1110002N22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 68550
Homologene: 11657
Scube1
Name: signal peptide, CUB domain, EGF-like 1
Synonyms: A630023E24Rik, 7330410C13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 64706
Homologene: 11224
Calm1
Name: calmodulin 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 12313
HGNC: HGNC:1442
Homologene: 116117
Rbl1
Name: RB transcriptional corepressor like 1
Synonyms: retinoblastoma-like 1 (p107), p107
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 19650
HGNC: HGNC:9893
Homologene: 2172
Dnah8
Name: dynein, axonemal, heavy chain 8
Synonyms: Hst6.7b, Dnahc8, P1-Loop
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 13417
VEGA: 17
HGNC: HGNC:2952
Homologene: 1049
Itsn2
Name: intersectin 2
Synonyms: Eh domain, SH3 domain regulator of endocytosis 2, Ese2, Sh3d1B, Sh3p18
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 20403
VEGA: 12
HGNC: HGNC:6184
Homologene: 22627
Cops8
Name: COP9 signalosome subunit 8
Synonyms: Csn8, Sgn8, 9430009J09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 108679
Homologene: 4882
Zfyve26
Name: zinc finger, FYVE domain containing 26
Synonyms: A630028O16Rik, LOC380767, 9330197E15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 211978
VEGA: 12
Homologene: 9102
Lsr
Name: lipolysis stimulated lipoprotein receptor
Synonyms: Lisch7, ILDR3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 54135
Homologene: 9306
Klf16
Name: Kruppel-like factor 16
Synonyms: BTEB4, DRRF
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 118445
Homologene: 136123
Tnrc6c
Name: trinucleotide repeat containing 6C
Synonyms: 9930033H14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217351
Homologene: 23137
Rapgef3
Name: Rap guanine nucleotide exchange factor (GEF) 3
Synonyms: Epac1, 9330170P05Rik, 2310016P22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 223864
Homologene: 21231
Ptpn13
Name: protein tyrosine phosphatase, non-receptor type 13
Synonyms: PTP-BL, PTPL1, Ptpri
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 19249
HGNC: HGNC:9646
Homologene: 7909
Arhgap20
Name: Rho GTPase activating protein 20
Synonyms: 6530403F17Rik, A530023E23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 244867
Homologene: 18938
Slc22a4
Name: solute carrier family 22 (organic cation transporter), member 4
Synonyms: Octn1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 30805
Homologene: 81701
Naip5
Name: NLR family, apoptosis inhibitory protein 5
Synonyms: Birc1e, Lgn1, Naip-rs3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 17951
HGNC: HGNC:7634
Homologene: 113589
Dnah12
Name: dynein, axonemal, heavy chain 12
Synonyms: DHC3, Dnahc7l, DLP12, Dnahc12, Hdhc3, 4921531P07Rik, LOC380889, HL19, HL-19
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 110083
HGNC: HGNC:2943
Homologene: 56821
Lrrc43
Name: leucine rich repeat containing 43
Synonyms: LOC381741
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 381741
Homologene: 17662
Man1a
Name: mannosidase 1, alpha
Synonyms: PCR1, mannosyl-oligosaccharide alpha-1,2-mannosidase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 17155
HGNC: HGNC:6821
Homologene: 4316
Ces2c
Name: carboxylesterase 2C
Synonyms: Ces2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 234671
HGNC: HGNC:1864
Homologene: 138298
Sel1l3
Name: sel-1 suppressor of lin-12-like 3 (C. elegans)
Synonyms: 2310045A20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231238
Homologene: 9054
Dhh
Name: desert hedgehog
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 13363
VEGA: 15
HGNC: HGNC:2865
Homologene: 22431
Padi6
Name: peptidyl arginine deiminase, type VI
Synonyms: Padi5, ePAD, Pad6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242726
Homologene: 17695
Klhdc7b
Name: kelch domain containing 7B
Synonyms: EG546648
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 546648
Homologene: 34707
Dnah7b
Name: dynein, axonemal, heavy chain 7B
Synonyms: LOC227058, Dnahc7b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227058
Homologene: 41287
Cacna1e
Name: calcium channel, voltage-dependent, R type, alpha 1E subunit
Synonyms: Cchra1, Cav2.3, alpha1E
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12290
HGNC: HGNC:1392
Homologene: 20185
Olfr638
Name: olfactory receptor 638
Synonyms: GA_x6K02T2PBJ9-6737723-6738670, MOR5-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 259124
Homologene: 133592
Slc26a7
Name: solute carrier family 26, member 7
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 208890
Homologene: 13770
Ehhadh
Name: enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase
Synonyms: MFP1, 1300002P22Rik, HD, MFP, L-bifunctional enzyme, L-PBE
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 74147
HGNC: HGNC:3247
Homologene: 1486
Pde6a
Name: phosphodiesterase 6A, cGMP-specific, rod, alpha
Synonyms: Pdea
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225600
HGNC: HGNC:8785
Homologene: 380
Gfra1
Name: glial cell line derived neurotrophic factor family receptor alpha 1
Synonyms: GFR alpha-1, GDNFR-alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 14585
HGNC: HGNC:4243
Homologene: 3855
Ttbk2
Name: tau tubulin kinase 2
Synonyms: 2610507N02Rik, B930008N24Rik, TTK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 140810
Homologene: 62795
Lcmt2
Name: leucine carboxyl methyltransferase 2
Synonyms: Tyw4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 329504
Homologene: 19642
Dgki
Name: diacylglycerol kinase, iota
Synonyms: C130010K08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 320127
HGNC: HGNC:2855
Homologene: 37956
Slc47a1
Name: solute carrier family 47, member 1
Synonyms: MATE1, 1300013J15Rik, mMATE1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 67473
Homologene: 34364
Ankrd44
Name: ankyrin repeat domain 44
Synonyms: E130014H08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 329154
Homologene: 27547
Cyp2d9
Name: cytochrome P450, family 2, subfamily d, polypeptide 9
Synonyms: Cyp2d, P450-2D, testosterone 16alpha-hydroxylase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 13105
VEGA: 15
Homologene: 86099
Acoxl
Name: acyl-Coenzyme A oxidase-like
Synonyms: 1200014P05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 74121
Homologene: 75083
Abcd2
Name: ATP-binding cassette, sub-family D (ALD), member 2
Synonyms: ALDL1, ALDR, ABC39, adrenoleukodystrophy related
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 26874
VEGA: 15
HGNC: HGNC:66
Homologene: 55873
Mmp24
Name: matrix metallopeptidase 24
Synonyms: Membrane type 5-MMP, MT5-MMP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17391
HGNC: HGNC:7172
Homologene: 21331
Ms4a3
Name: membrane-spanning 4-domains, subfamily A, member 3
Synonyms: haematopoietic cell-specific transmembrane-4, HTm4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 170813
HGNC: HGNC:7317
Homologene: 4472
Lrp11
Name: low density lipoprotein receptor-related protein 11
Synonyms: 9830160H19Rik, 1700034J19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 237253
Homologene: 13133
C4bp
Name: complement component 4 binding protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12269
Zfp36l2
Name: zinc finger protein 36, C3H type-like 2
Synonyms: Brf2, ERF2, Tis11d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 12193
HGNC: HGNC:1108
Homologene: 5027
Chil4
Name: chitinase-like 4
Synonyms: Chi3l4, Ym2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 104183
Homologene: 74931
Ifna4
Name: interferon alpha 4
Synonyms: Ifa4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 15967
Btd
Name: biotinidase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 26363
HGNC: HGNC:1122
Homologene: 37255
Olfr885
Name: olfactory receptor 885
Synonyms: GA_x6K02T2PVTD-31740639-31741568, MOR162-12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 257885
Homologene: 79400
Gm5327
Name: predicted pseudogene 5327
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 384591
Ighv2-1
Name: immunoglobulin heavy variable 2-1
Synonyms: Gm17347
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 777684
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to A, chromosome 1 at 46,207,656 bp
  • A to T, chromosome 1 at 54,667,417 bp
  • A to G, chromosome 1 at 90,605,427 bp
  • A to G, chromosome 1 at 130,639,185 bp
  • G to A, chromosome 1 at 154,436,468 bp
  • A to G, chromosome 1 at 172,369,699 bp
  • A to G, chromosome 2 at 61,653,532 bp
  • T to C, chromosome 2 at 120,745,370 bp
  • C to G, chromosome 2 at 121,139,430 bp
  • T to G, chromosome 2 at 127,978,738 bp
  • C to T, chromosome 2 at 155,815,899 bp
  • T to C, chromosome 2 at 157,170,003 bp
  • A to G, chromosome 3 at 40,805,154 bp
  • C to T, chromosome 3 at 106,210,524 bp
  • C to T, chromosome 4 at 14,590,477 bp
  • A to G, chromosome 4 at 40,807,812 bp
  • C to A, chromosome 4 at 88,842,282 bp
  • G to A, chromosome 4 at 109,840,367 bp
  • T to G, chromosome 4 at 140,731,195 bp
  • C to A, chromosome 5 at 53,144,183 bp
  • T to C, chromosome 5 at 101,930,028 bp
  • T to A, chromosome 5 at 103,569,855 bp
  • A to T, chromosome 5 at 123,494,303 bp
  • C to A, chromosome 6 at 37,299,813 bp
  • T to C, chromosome 7 at 30,966,040 bp
  • A to G, chromosome 7 at 32,546,717 bp
  • C to T, chromosome 7 at 45,172,934 bp
  • T to G, chromosome 7 at 104,003,890 bp
  • T to C, chromosome 7 at 138,898,706 bp
  • A to T, chromosome 8 at 25,818,595 bp
  • T to C, chromosome 8 at 104,848,040 bp
  • T to C, chromosome 9 at 31,403,623 bp
  • T to C, chromosome 9 at 38,062,093 bp
  • T to G, chromosome 9 at 51,785,837 bp
  • A to G, chromosome 10 at 5,001,418 bp
  • A to G, chromosome 10 at 7,590,584 bp
  • A to T, chromosome 10 at 53,907,572 bp
  • T to C, chromosome 10 at 80,569,186 bp
  • T to G, chromosome 10 at 100,563,270 bp
  • T to A, chromosome 11 at 54,027,651 bp
  • T to C, chromosome 11 at 61,363,451 bp
  • T to C, chromosome 11 at 80,140,453 bp
  • G to C, chromosome 11 at 86,544,658 bp
  • T to A, chromosome 11 at 117,722,571 bp
  • A to G, chromosome 12 at 4,707,660 bp
  • A to T, chromosome 12 at 79,244,396 bp
  • T to C, chromosome 12 at 100,200,226 bp
  • A to G, chromosome 12 at 113,574,470 bp
  • C to A, chromosome 13 at 100,221,870 bp
  • A to G, chromosome 14 at 26,872,317 bp
  • A to G, chromosome 14 at 31,662,321 bp
  • A to G, chromosome 14 at 37,133,743 bp
  • G to A, chromosome 15 at 81,729,755 bp
  • T to C, chromosome 15 at 82,454,401 bp
  • G to A, chromosome 15 at 83,616,780 bp
  • T to A, chromosome 15 at 89,387,582 bp
  • T to C, chromosome 15 at 91,178,286 bp
  • A to G, chromosome 15 at 97,760,600 bp
  • A to G, chromosome 15 at 98,898,142 bp
  • T to C, chromosome 16 at 21,762,431 bp
  • T to C, chromosome 17 at 30,851,747 bp
  • T to C, chromosome 17 at 51,804,347 bp
  • T to A, chromosome 17 at 84,187,661 bp
  • T to A, chromosome 18 at 31,888,447 bp
  • A to G, chromosome 18 at 61,231,489 bp
  • A to G, chromosome 19 at 11,631,378 bp
  • A to T, chromosome 19 at 58,263,954 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4727 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041602-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.