Loading Mouse GIF
Loading...

Strain Name:
C57BL/6J-MtgxR4049Btlr/Mmmh
Stock Number:
041616-MU
Citation ID:
RRID:MMRRC_041616-MU
Other Names:
R4049 (G1), C57BL/6J-MtgxR4049Btlr
Major Collection:

Strain Information

Ncor1
Name: nuclear receptor co-repressor 1
Synonyms: N-CoR, Rxrip13, A230020K14Rik, 5730405M06Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20185
HGNC: HGNC:7672
Homologene: 38166
Rasgrp2
Name: RAS, guanyl releasing protein 2
Synonyms: CalDAG-GEFI, Caldaggef1
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 19395
HGNC: HGNC:9879
Homologene: 4250
Csf2
Name: colony stimulating factor 2 (granulocyte-macrophage)
Synonyms: Gm-CSf, Csfgm, MGI-IGM, GMCSF
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 12981
HGNC: HGNC:2434
Homologene: 600
Herc3
Name: hect domain and RLD 3
Synonyms: 5730409F18Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 73998
HGNC: HGNC:4876
Homologene: 57095
Blm
Name: Bloom syndrome, RecQ like helicase
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 12144
HGNC: HGNC:1058
Homologene: 47902
Mad1l1
Name: MAD1 mitotic arrest deficient 1-like 1
Synonyms: Mad1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 17120
HGNC: HGNC:6762
Homologene: 74500
Rai14
Name: retinoic acid induced 14
Synonyms: 1700020L11Rik, Ankycorbin, Norpeg, 1700008J19Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 75646
VEGA: 15
Homologene: 9199
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 20,553,608 bp
  • A to G, chromosome 1 at 88,258,664 bp
  • T to C, chromosome 1 at 135,254,277 bp
  • T to G, chromosome 1 at 175,805,856 bp
  • T to C, chromosome 2 at 88,343,800 bp
  • A to G, chromosome 2 at 88,959,273 bp
  • T to C, chromosome 2 at 89,667,662 bp
  • C to T, chromosome 2 at 132,060,683 bp
  • T to C, chromosome 2 at 132,381,494 bp
  • G to T, chromosome 2 at 155,277,542 bp
  • A to G, chromosome 2 at 163,828,984 bp
  • C to T, chromosome 3 at 5,398,859 bp
  • A to C, chromosome 3 at 29,686,731 bp
  • C to T, chromosome 4 at 130,068,752 bp
  • T to C, chromosome 4 at 131,900,543 bp
  • T to A, chromosome 4 at 140,515,451 bp
  • A to G, chromosome 4 at 145,702,600 bp
  • T to C, chromosome 4 at 147,826,512 bp
  • T to A, chromosome 5 at 62,827,165 bp
  • T to C, chromosome 5 at 114,412,870 bp
  • T to A, chromosome 5 at 122,122,750 bp
  • A to G, chromosome 5 at 140,132,816 bp
  • A to G, chromosome 5 at 145,713,540 bp
  • A to G, chromosome 6 at 58,876,837 bp
  • A to T, chromosome 6 at 67,466,433 bp
  • T to C, chromosome 6 at 140,583,871 bp
  • T to A, chromosome 7 at 19,151,307 bp
  • T to A, chromosome 7 at 80,502,862 bp
  • T to C, chromosome 7 at 104,580,368 bp
  • C to T, chromosome 7 at 111,055,543 bp
  • A to G, chromosome 8 at 4,260,561 bp
  • A to G, chromosome 8 at 14,979,998 bp
  • T to A, chromosome 8 at 22,243,164 bp
  • A to G, chromosome 8 at 26,979,085 bp
  • A to T, chromosome 8 at 70,629,955 bp
  • G to A, chromosome 9 at 78,322,336 bp
  • A to T, chromosome 9 at 95,638,755 bp
  • T to G, chromosome 9 at 97,457,560 bp
  • G to A, chromosome 10 at 6,829,087 bp
  • G to T, chromosome 10 at 41,729,360 bp
  • A to T, chromosome 10 at 130,447,097 bp
  • C to A, chromosome 11 at 21,300,183 bp
  • C to T, chromosome 11 at 29,838,577 bp
  • A to G, chromosome 11 at 54,249,333 bp
  • G to T, chromosome 11 at 62,329,668 bp
  • A to G, chromosome 11 at 78,075,602 bp
  • T to A, chromosome 11 at 87,140,603 bp
  • G to A, chromosome 11 at 117,778,261 bp
  • A to C, chromosome 11 at 119,482,448 bp
  • T to C, chromosome 12 at 86,012,799 bp
  • T to A, chromosome 12 at 118,868,669 bp
  • T to C, chromosome 13 at 14,053,699 bp
  • T to C, chromosome 14 at 54,373,091 bp
  • T to C, chromosome 15 at 10,592,212 bp
  • G to A, chromosome 15 at 44,498,557 bp
  • T to C, chromosome 15 at 73,522,215 bp
  • T to C, chromosome 15 at 80,560,039 bp
  • A to G, chromosome 15 at 82,853,429 bp
  • T to A, chromosome 15 at 88,995,032 bp
  • C to A, chromosome 15 at 93,465,106 bp
  • C to T, chromosome 15 at 103,250,937 bp
  • A to G, chromosome 15 at 103,532,454 bp
  • A to T, chromosome 16 at 96,013,885 bp
  • A to G, chromosome 17 at 80,605,965 bp
  • C to A, chromosome 18 at 60,832,903 bp
  • G to T, chromosome 18 at 67,529,031 bp
  • T to C, chromosome 19 at 6,404,727 bp
  • T to A, chromosome 19 at 12,429,689 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4049 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041616-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.