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Strain Name:
C57BL/6J-MtgxR4093Btlr/Mmmh
Stock Number:
041627-MU
Citation ID:
RRID:MMRRC_041627-MU
Other Names:
R4093 (G1), C57BL/6J-MtgxR4093Btlr
Major Collection:

Strain Information

Htr2a
Name: 5-hydroxytryptamine (serotonin) receptor 2A
Synonyms: 5-HT2A receptor, Htr-2, Htr2
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 15558
VEGA: 14
HGNC: HGNC:5293
Homologene: 68073
Zdbf2
Name: zinc finger, DBF-type containing 2
Synonyms: 9330107J05Rik, 4930431J08Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 73884
Homologene: 52868
Wfs1
Name: wolframin ER transmembrane glycoprotein
Synonyms: wolframin, Wolfram syndrome 1 homolog (human)
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 22393
Homologene: 4380
Ltbp4
Name: latent transforming growth factor beta binding protein 4
Synonyms: 2310046A13Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 108075
HGNC: HGNC:6717
Homologene: 2645
Sfswap
Name: splicing factor SWAP
Synonyms: 6330437E22Rik, 1190005N23Rik, Sfrs8
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 231769
Homologene: 134548
Proser1
Name: proline and serine rich 1
Synonyms: 2810046L04Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 212127
Homologene: 13463
Akap9
Name: A kinase anchor protein 9
Synonyms: AKAP450, 5730481H23Rik, G1-448-15, repro12, mei2-5
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 100986
HGNC: HGNC:379
Homologene: 134583
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to A, chromosome 1 at 36,344,954 bp
  • T to C, chromosome 1 at 63,309,781 bp
  • C to T, chromosome 1 at 87,191,007 bp
  • T to C, chromosome 1 at 193,181,364 bp
  • A to T, chromosome 2 at 25,877,915 bp
  • G to A, chromosome 2 at 29,377,908 bp
  • A to T, chromosome 2 at 32,387,716 bp
  • A to T, chromosome 2 at 34,983,807 bp
  • T to A, chromosome 2 at 121,195,477 bp
  • T to C, chromosome 2 at 168,925,935 bp
  • A to T, chromosome 2 at 181,811,398 bp
  • C to A, chromosome 3 at 30,695,522 bp
  • T to C, chromosome 3 at 53,479,712 bp
  • T to C, chromosome 3 at 65,299,614 bp
  • C to A, chromosome 3 at 89,433,264 bp
  • A to T, chromosome 3 at 93,727,237 bp
  • A to G, chromosome 3 at 107,214,266 bp
  • T to A, chromosome 4 at 107,843,271 bp
  • A to G, chromosome 5 at 4,043,996 bp
  • T to C, chromosome 5 at 36,967,465 bp
  • T to A, chromosome 5 at 119,680,748 bp
  • A to G, chromosome 5 at 129,560,741 bp
  • A to G, chromosome 6 at 42,642,838 bp
  • T to C, chromosome 6 at 83,289,164 bp
  • T to C, chromosome 6 at 94,613,578 bp
  • T to C, chromosome 7 at 4,483,367 bp
  • G to T, chromosome 7 at 5,220,857 bp
  • T to A, chromosome 7 at 5,675,944 bp
  • A to G, chromosome 7 at 7,430,337 bp
  • C to A, chromosome 7 at 9,942,258 bp
  • T to A, chromosome 7 at 9,942,259 bp
  • A to G, chromosome 7 at 24,291,275 bp
  • A to T, chromosome 7 at 26,611,783 bp
  • A to G, chromosome 7 at 27,325,216 bp
  • A to T, chromosome 7 at 43,938,780 bp
  • C to T, chromosome 7 at 73,501,016 bp
  • T to C, chromosome 7 at 85,621,234 bp
  • A to T, chromosome 7 at 96,895,772 bp
  • C to T, chromosome 7 at 104,996,635 bp
  • A to G, chromosome 7 at 126,866,871 bp
  • T to C, chromosome 8 at 28,844,837 bp
  • A to T, chromosome 8 at 65,001,378 bp
  • A to G, chromosome 8 at 109,251,459 bp
  • G to A, chromosome 8 at 119,749,871 bp
  • A to C, chromosome 8 at 122,501,160 bp
  • T to A, chromosome 9 at 38,464,083 bp
  • T to C, chromosome 9 at 95,706,865 bp
  • G to T, chromosome 9 at 106,241,801 bp
  • T to C, chromosome 10 at 41,486,937 bp
  • A to G, chromosome 11 at 16,259,884 bp
  • A to T, chromosome 11 at 58,566,829 bp
  • C to A, chromosome 11 at 96,346,100 bp
  • T to A, chromosome 11 at 98,650,851 bp
  • A to G, chromosome 11 at 115,904,888 bp
  • A to G, chromosome 12 at 4,865,241 bp
  • A to G, chromosome 12 at 104,037,924 bp
  • G to A, chromosome 12 at 114,708,730 bp
  • A to T, chromosome 14 at 20,409,113 bp
  • T to C, chromosome 14 at 34,147,141 bp
  • A to T, chromosome 14 at 55,588,277 bp
  • G to A, chromosome 14 at 74,706,349 bp
  • T to C, chromosome 15 at 42,523,545 bp
  • T to C, chromosome 15 at 98,437,682 bp
  • A to G, chromosome 16 at 4,497,452 bp
  • A to C, chromosome 16 at 16,829,024 bp
  • T to C, chromosome 16 at 49,000,976 bp
  • A to G, chromosome 16 at 66,784,788 bp
  • A to T, chromosome 17 at 7,929,423 bp
  • C to T, chromosome 17 at 12,612,394 bp
  • T to C, chromosome 17 at 20,583,380 bp
  • T to A, chromosome 17 at 21,992,982 bp
  • T to C, chromosome 17 at 33,749,789 bp
  • T to C, chromosome 17 at 74,445,958 bp
  • A to T, chromosome 18 at 43,475,126 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4093 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041627-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.