Strain Name:
C57BL/6J-MtgxR4319Btlr/Mmmh
Stock Number:
041660-MU
Citation ID:
RRID:MMRRC_041660-MU
Other Names:
R4319 (G1), C57BL/6J-MtgxR4319Btlr
Major Collection:

Gene Information

Pygb
Name: brain glycogen phosphorylase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 110078
HGNC: HGNC:9723
Homologene: 100930
Klf2
Name: Kruppel-like factor 2 (lung)
Synonyms: Lklf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 16598
HGNC: HGNC:6347
Homologene: 133978
Itsn1
Name: intersectin 1 (SH3 domain protein 1A)
Synonyms: Eh domain, SH3 domain regulator of endocytosis 1, EHSH1, Sh3p17, Ese1, Intersectin-L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 16443
HGNC: HGNC:6183
Homologene: 2277
Nf1
Name: neurofibromin 1
Synonyms: Mhdadsk9, Nf-1, Dsk9, neurofibromin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 18015
HGNC: HGNC:7765
Homologene: 226
Kif11
Name: kinesin family member 11
Synonyms: Eg5, Kifl1, Knsl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 16551
VEGA: 19
HGNC: HGNC:6388
Homologene: 3322
Cep290
Name: centrosomal protein 290
Synonyms: b2b1454Clo, Nphp6, Kiaa, b2b1752Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 216274
VEGA: 10
Homologene: 77213
Srbd1
Name: S1 RNA binding domain 1
Synonyms: D530025C17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 78586
VEGA: 17
Homologene: 9994
Ccdc191
Name: coiled-coil domain containing 191
Synonyms: 2610015P09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 212153
Homologene: 19484
Luc7l
Name: Luc7-like
Synonyms: 1810045C04Rik, 2410018D03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 66978
HGNC: HGNC:6723
Homologene: 100558
Mapk11
Name: mitogen-activated protein kinase 11
Synonyms: Prkm11, P38b, Sapk2, p38beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 19094
VEGA: 15
HGNC: HGNC:6873
Homologene: 55684
Cacna1c
Name: calcium channel, voltage-dependent, L type, alpha 1C subunit
Synonyms: (alpha)1 subunit, Cchl1a1, Cav1.2, L-type Cav1.2, D930026N18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12288
HGNC: HGNC:1390
Homologene: 55484
Sox6
Name: SRY (sex determining region Y)-box 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 20679
Homologene: 22631
Mcoln2
Name: mucolipin 2
Synonyms: mucolipidin 2, TRPML2, 3300002C04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 68279
Homologene: 12258
Chrd
Name: chordin
Synonyms: Chd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 12667
HGNC: HGNC:1949
Homologene: 2774
Dgkb
Name: diacylglycerol kinase, beta
Synonyms: C630029D13Rik, DGK-beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217480
VEGA: 12
HGNC: HGNC:2850
Homologene: 37875
Vmn2r63
Name: vomeronasal 2, receptor 63
Synonyms: EG435975
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 435975
Homologene: 104832
Rufy4
Name: RUN and FYVE domain containing 4
Synonyms: F930048N03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 435626
Homologene: 52359
Ccdc178
Name: coiled coil domain containing 178
Synonyms: 4921528I01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 70950
VEGA: 18
Homologene: 12373
Wdr46
Name: WD repeat domain 46
Synonyms: 2310007I04Rik, Bing4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 57315
Homologene: 3981
Spty2d1
Name: SPT2 chromatin protein domain containing 1
Synonyms: 5830435K17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 101685
Homologene: 45499
Ufsp2
Name: UFM1-specific peptidase 2
Synonyms: 1810047C23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 192169
Homologene: 10151
Pira13
Name: paired-Ig-like receptor A13
Synonyms: ENSMUSG00000074419, Gm15448
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100041146
HGNC: HGNC:6607
Homologene: 134028
Olfr1392
Name: olfactory receptor 1392
Synonyms: GA_x6K02T2QP88-6141322-6140387, MOR256-25
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 258462
Homologene: 81347
Alpk1
Name: alpha-kinase 1
Synonyms: 8430410J10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 71481
Homologene: 11849
Tspo2
Name: translocator protein 2
Synonyms: 2510027D20Rik, Bzrpl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 70026
Homologene: 12344
Atxn7l2
Name: ataxin 7-like 2
Synonyms: 2610528J18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 72522
Homologene: 17728
Bahd1
Name: bromo adjacent homology domain containing 1
Synonyms: LOC228536
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228536
Homologene: 8976
Gm2663
Name: predicted gene 2663
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 100040208
Homologene: 117995
Gm26724
Name: predicted gene, 26724
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 74,147,663 bp
  • C to T, chromosome 2 at 118,916,406 bp
  • G to T, chromosome 2 at 150,815,614 bp
  • A to T, chromosome 3 at 108,205,832 bp
  • A to G, chromosome 3 at 127,681,435 bp
  • G to A, chromosome 3 at 146,150,011 bp
  • G to T, chromosome 6 at 40,997,596 bp
  • A to C, chromosome 6 at 118,654,369 bp
  • A to G, chromosome 7 at 3,822,755 bp
  • A to G, chromosome 7 at 42,926,982 bp
  • T to C, chromosome 7 at 46,998,135 bp
  • A to G, chromosome 7 at 115,580,563 bp
  • A to G, chromosome 8 at 45,995,627 bp
  • A to G, chromosome 8 at 72,320,161 bp
  • A to C, chromosome 10 at 100,539,047 bp
  • G to A, chromosome 11 at 49,293,676 bp
  • T to A, chromosome 11 at 79,418,584 bp
  • A to G, chromosome 12 at 38,438,599 bp
  • T to C, chromosome 15 at 89,146,743 bp
  • A to G, chromosome 16 at 20,737,048 bp
  • A to G, chromosome 16 at 43,947,509 bp
  • G to A, chromosome 16 at 91,818,552 bp
  • A to T, chromosome 17 at 26,277,619 bp
  • A to G, chromosome 17 at 27,201,677 bp
  • A to G, chromosome 17 at 33,940,744 bp
  • G to T, chromosome 17 at 48,449,843 bp
  • A to T, chromosome 17 at 86,051,150 bp
  • T to A, chromosome 18 at 22,033,543 bp
  • T to C, chromosome 19 at 37,384,585 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4319 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041660-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.