Strain Name:
C57BL/6J-MtgxR4321Btlr/Mmmh
Stock Number:
041662-MU
Citation ID:
RRID:MMRRC_041662-MU
Other Names:
R4321 (G1), C57BL/6J-MtgxR4321Btlr
Major Collection:

Gene Information

Myo5a
Name: myosin VA
Synonyms: MVa, MyoVA, Myo5, flail, 9630007J19Rik, Dbv
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 17918
HGNC: HGNC:7602
Homologene: 20100
Setd5
Name: SET domain containing 5
Synonyms: 2900045N06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 72895
Homologene: 12485
Epha4
Name: Eph receptor A4
Synonyms: Sek, Sek1, Tyro1, Hek8, Cek8, 2900005C20Rik, rb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 13838
HGNC: HGNC:3388
Homologene: 20933
Egf
Name: epidermal growth factor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 13645
HGNC: HGNC:3229
Homologene: 1483
Gaa
Name: glucosidase, alpha, acid
Synonyms: E430018M07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 14387
HGNC: HGNC:4065
Homologene: 37268
Orc1
Name: origin recognition complex, subunit 1
Synonyms: MmORC1, Orc1l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 18392
HGNC: HGNC:8487
Homologene: 31221
Itsn1
Name: intersectin 1 (SH3 domain protein 1A)
Synonyms: EHSH1, Sh3p17, Ese1, Eh domain, SH3 domain regulator of endocytosis 1, Intersectin-L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 16443
HGNC: HGNC:6183
Homologene: 2277
Golga4
Name: golgi autoantigen, golgin subfamily a, 4
Synonyms: golgin-245, Olp-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 54214
HGNC: HGNC:4427
Homologene: 68224
Txnl1
Name: thioredoxin-like 1
Synonyms: TRP32, 32kDa
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 53382
VEGA: 18
Homologene: 3515
Cep85
Name: centrosomal protein 85
Synonyms: 2410030J07Rik, Ccdc21
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 70012
Homologene: 11254
Lonp2
Name: lon peptidase 2, peroxisomal
Synonyms: 1300002A08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 66887
Homologene: 12050
Dock7
Name: dedicator of cytokinesis 7
Synonyms: 3110056M06Rik, LOC242555, m
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 67299
Homologene: 23566
Leng8
Name: leukocyte receptor cluster (LRC) member 8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 232798
Homologene: 13612
Gad1
Name: glutamate decarboxylase 1
Synonyms: GAD67, Gad-1, Z49976, EP10, GAD44, GAD25
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 14415
HGNC: HGNC:4092
Homologene: 635
Lama1
Name: laminin, alpha 1
Synonyms: Lama
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 16772
VEGA: 17
HGNC: HGNC:6481
Homologene: 21146
Ibtk
Name: inhibitor of Bruton agammaglobulinemia tyrosine kinase
Synonyms: 5430411K16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 108837
Homologene: 34661
Ice1
Name: interactor of little elongation complex ELL subunit 1
Synonyms: BC018507
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 218333
VEGA: 13
Homologene: 18902
Cpped1
Name: calcineurin-like phosphoesterase domain containing 1
Synonyms: C530044N13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 223978
Homologene: 10142
Itpr3
Name: inositol 1,4,5-triphosphate receptor 3
Synonyms: Itpr-3, Ip3r3, tf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 16440
HGNC: HGNC:6182
Homologene: 1675
Sox6
Name: SRY (sex determining region Y)-box 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 20679
Homologene: 22631
Dok7
Name: docking protein 7
Synonyms: EF-12, A930013K19Rik, Dok-7, Oit5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231134
Homologene: 18210
Camta2
Name: calmodulin binding transcription activator 2
Synonyms: Kiaa0909-hp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216874
Homologene: 9021
Mlxipl
Name: MLX interacting protein-like
Synonyms: WS-bHLH, ChREBP, Wbscr14, bHLHd14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 58805
Homologene: 32507
Myo3a
Name: myosin IIIA
Synonyms: 9030416P08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 667663
HGNC: HGNC:7601
Homologene: 49486
Ulk4
Name: unc-51-like kinase 4
Synonyms: 4932415A06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 209012
Homologene: 41205
Wdr64
Name: WD repeat domain 64
Synonyms: 4930415O10Rik, 4930511H01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 75820
Homologene: 51634
Kprp
Name: keratinocyte expressed, proline-rich
Synonyms: 1110001M24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 433619
Homologene: 54921
Vmn2r63
Name: vomeronasal 2, receptor 63
Synonyms: EG435975
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 435975
Homologene: 104832
Rufy2
Name: RUN and FYVE domain-containing 2
Synonyms: LZ-FYVE, 2610111M19Rik, Denn, ZFYVE13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 70432
Homologene: 23064
Tshz2
Name: teashirt zinc finger family member 2
Synonyms: Mtsh2, Sdccag33l, 2900073F20Rik, Zfp218, teashirt2, Tsh2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228911
Homologene: 35258
Rufy4
Name: RUN and FYVE domain containing 4
Synonyms: F930048N03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 435626
Homologene: 52359
Dthd1
Name: death domain containing 1
Synonyms: Gm17384
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100322896
Homologene: 123510
Nrxn3
Name: neurexin III
Synonyms: neurexin III beta, neurexin III alpha, neurexin III beta, neurexin III alpha, 9330112C09Rik, D12Bwg0831e, 4933401A11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 18191
HGNC: HGNC:8010
Homologene: 83225
Bbof1
Name: basal body orientation factor 1
Synonyms: 2900006K08Rik, Ccdc176
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 72873
Homologene: 75215
Spef2
Name: sperm flagellar 2
Synonyms: C230086A09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 320277
Homologene: 23371
Ccdc178
Name: coiled coil domain containing 178
Synonyms: 4921528I01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 70950
VEGA: 18
Homologene: 12373
Hipk3
Name: homeodomain interacting protein kinase 3
Synonyms: DYRK6, FIST3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 15259
HGNC: HGNC:4915
Homologene: 55923
Myorg
Name: myogenesis regulating glycosidase (putative)
Synonyms: NET37, AI464131
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 329828
Homologene: 19853
9330159F19Rik
Name: RIKEN cDNA 9330159F19 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 212448
VEGA: 10
Homologene: 8804
Mark1
Name: MAP/microtubule affinity regulating kinase 1
Synonyms: Emk3, B930025N23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 226778
HGNC: HGNC:6896
Homologene: 49552
Daxx
Name: Fas death domain-associated protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 13163
HGNC: HGNC:2681
Homologene: 1033
Fbxw13
Name: F-box and WD-40 domain protein 13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 211305
Homologene: 110776
Aoc1l1
Name: amine oxidase copper containing 1-like 1
Synonyms: Doxl2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 243376
Homologene: 19443
Pira13
Name: paired-Ig-like receptor A13
Synonyms: ENSMUSG00000074419, Gm15448
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100041146
HGNC: HGNC:6607
Homologene: 134028
Slamf1
Name: signaling lymphocytic activation molecule family member 1
Synonyms: IPO-3, CD150, ESTM51, CDw150, Slam
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 27218
Homologene: 48162
Clvs1
Name: clavesin 1
Synonyms: 4933402J24Rik, Rlbp1l1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 74438
Homologene: 65267
Ggcx
Name: gamma-glutamyl carboxylase
Synonyms: vitamin K-dependent carboxylase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 56316
HGNC: HGNC:4247
Homologene: 639
Septin1
Name: septin 1
Synonyms: PNUTL3, Diff6, Sept1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 54204
HGNC: HGNC:2879
Homologene: 23009
Zfp850
Name: zinc finger protein 850
Synonyms: C130069I09Rik, Gm4636
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 100043772
Gm2663
Name: predicted gene 2663
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 100040208
Homologene: 117995
Vamp8
Name: vesicle-associated membrane protein 8
Synonyms: endobrevin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 22320
Homologene: 37846
Prox2
Name: prospero homeobox 2
Synonyms: 1700058C01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 73422
Homologene: 18421
Gm29017
Name: predicted gene 29017
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Ankrd9
Name: ankyrin repeat domain 9
Synonyms: 2500003O20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 74251
Homologene: 17549
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to C, chromosome 1 at 59,167,454 bp
  • A to T, chromosome 1 at 74,132,784 bp
  • T to C, chromosome 1 at 74,147,663 bp
  • T to A, chromosome 1 at 77,507,213 bp
  • T to C, chromosome 1 at 171,775,126 bp
  • T to C, chromosome 1 at 175,726,484 bp
  • G to T, chromosome 1 at 184,898,674 bp
  • A to T, chromosome 2 at 22,267,155 bp
  • G to A, chromosome 2 at 70,589,830 bp
  • C to A, chromosome 2 at 104,446,571 bp
  • T to A, chromosome 2 at 169,885,545 bp
  • G to A, chromosome 3 at 92,824,856 bp
  • A to G, chromosome 3 at 129,706,134 bp
  • T to C, chromosome 4 at 9,282,029 bp
  • C to T, chromosome 4 at 41,498,767 bp
  • T to C, chromosome 4 at 99,072,454 bp
  • G to A, chromosome 4 at 108,588,776 bp
  • G to A, chromosome 4 at 134,132,285 bp
  • A to T, chromosome 5 at 35,079,797 bp
  • A to T, chromosome 5 at 62,818,690 bp
  • C to T, chromosome 5 at 135,135,450 bp
  • G to T, chromosome 6 at 40,997,596 bp
  • T to A, chromosome 6 at 48,976,522 bp
  • A to C, chromosome 6 at 72,385,553 bp
  • A to G, chromosome 6 at 72,428,820 bp
  • T to A, chromosome 6 at 113,143,735 bp
  • A to G, chromosome 7 at 3,822,755 bp
  • T to A, chromosome 7 at 4,143,554 bp
  • A to C, chromosome 7 at 27,989,400 bp
  • A to G, chromosome 7 at 42,926,982 bp
  • A to G, chromosome 7 at 115,580,563 bp
  • G to A, chromosome 7 at 127,217,028 bp
  • A to G, chromosome 8 at 86,665,728 bp
  • T to G, chromosome 9 at 75,217,530 bp
  • T to C, chromosome 9 at 85,735,072 bp
  • A to C, chromosome 9 at 109,181,435 bp
  • A to G, chromosome 9 at 118,556,435 bp
  • T to G, chromosome 9 at 121,073,996 bp
  • A to T, chromosome 10 at 29,224,891 bp
  • A to G, chromosome 10 at 62,982,680 bp
  • A to T, chromosome 11 at 70,678,325 bp
  • A to T, chromosome 11 at 119,270,137 bp
  • A to T, chromosome 12 at 84,427,128 bp
  • A to G, chromosome 12 at 85,095,398 bp
  • A to T, chromosome 12 at 90,199,231 bp
  • A to G, chromosome 12 at 110,976,640 bp
  • A to T, chromosome 13 at 70,603,110 bp
  • A to T, chromosome 15 at 9,679,343 bp
  • T to C, chromosome 16 at 11,887,746 bp
  • G to A, chromosome 16 at 91,818,552 bp
  • A to G, chromosome 17 at 27,111,974 bp
  • T to C, chromosome 17 at 33,911,406 bp
  • G to A, chromosome 17 at 67,771,083 bp
  • T to A, chromosome 18 at 22,033,543 bp
  • T to C, chromosome 18 at 63,679,490 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4321 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041662-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.