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Strain Name:
C57BL/6J-MtgxR4395Btlr/Mmmh
Stock Number:
041684-MU
Citation ID:
RRID:MMRRC_041684-MU
Other Names:
R4395 (G1), C57BL/6J-MtgxR4395Btlr
Major Collection:

Strain Information

Tle4
Name: transducin-like enhancer of split 4
Synonyms: Grg4, ESTM13, ESTM14, Bce1, 5730411M05Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 21888
VEGA: 19
Homologene: 38259
Slc4a7
Name: solute carrier family 4, sodium bicarbonate cotransporter, member 7
Synonyms: E430014N10Rik, NBC3, NBCn1
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 218756
VEGA: 14
Homologene: 2680
Ndufv1
Name: NADH:ubiquinone oxidoreductase core subunit V1
Synonyms: 24 kDa (FP)
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 17995
HGNC: HGNC:7716
Homologene: 5151
Stk17b
Name: serine/threonine kinase 17b (apoptosis-inducing)
Synonyms: Drak2, 3110009A03Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 98267
Homologene: 31231
Tenm2
Name: teneurin transmembrane protein 2
Synonyms: Ten-m2, D3Bwg1534e, 9330187F13Rik, 2610040L17Rik, Odz2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 23964
Homologene: 22672
Rbm34
Name: RNA binding motif protein 34
Synonyms: 4930547K05Rik, D8Ertd233e
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 52202
Homologene: 56691
Capza2
Name: capping actin protein of muscle Z-line subunit alpha 2
Synonyms: Cappa2, 1110053K06Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 12343
HGNC: HGNC:1490
Homologene: 55956
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 53,764,115 bp
  • CAGAGAGAGAGAGA to CAGAGAGAGAGA, chromosome 1 at 88,211,984 bp
  • A to G, chromosome 2 at 31,931,952 bp
  • G to A, chromosome 2 at 118,922,523 bp
  • G to A, chromosome 3 at 106,203,727 bp
  • A to G, chromosome 4 at 49,447,679 bp
  • G to T, chromosome 4 at 134,116,120 bp
  • T to C, chromosome 5 at 30,918,135 bp
  • A to T, chromosome 5 at 148,076,622 bp
  • T to C, chromosome 6 at 17,656,450 bp
  • T to A, chromosome 6 at 27,429,432 bp
  • A to G, chromosome 6 at 134,849,245 bp
  • T to C, chromosome 6 at 141,712,118 bp
  • T to C, chromosome 7 at 28,826,246 bp
  • C to T, chromosome 7 at 81,094,955 bp
  • A to G, chromosome 7 at 107,845,262 bp
  • T to A, chromosome 8 at 44,952,346 bp
  • G to A, chromosome 8 at 70,290,143 bp
  • T to A, chromosome 8 at 88,664,391 bp
  • T to C, chromosome 8 at 126,949,381 bp
  • A to G, chromosome 9 at 48,340,613 bp
  • T to C, chromosome 10 at 13,190,579 bp
  • A to G, chromosome 10 at 18,597,709 bp
  • T to C, chromosome 10 at 29,147,355 bp
  • T to C, chromosome 10 at 77,929,219 bp
  • A to G, chromosome 10 at 80,344,902 bp
  • C to T, chromosome 10 at 120,476,051 bp
  • C to T, chromosome 11 at 36,024,624 bp
  • A to G, chromosome 11 at 97,688,386 bp
  • T to C, chromosome 12 at 32,204,092 bp
  • C to T, chromosome 14 at 14,765,665 bp
  • T to C, chromosome 15 at 76,352,157 bp
  • C to T, chromosome 15 at 88,915,580 bp
  • C to T, chromosome 15 at 102,108,934 bp
  • A to T, chromosome 16 at 21,898,571 bp
  • A to G, chromosome 16 at 59,560,095 bp
  • C to A, chromosome 16 at 97,481,786 bp
  • C to T, chromosome 17 at 34,678,662 bp
  • T to A, chromosome 19 at 4,008,837 bp
  • A to G, chromosome 19 at 8,896,444 bp
  • A to T, chromosome 19 at 13,780,911 bp
  • A to T, chromosome 19 at 14,517,938 bp
  • G to T, chromosome 19 at 34,612,551 bp
  • A to G, chromosome 19 at 56,479,894 bp
  • T to G, chromosome X at 56,932,343 bp
  • C to T, chromosome X at 152,209,053 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4395 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041684-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.