Strain Name:
C57BL/6J-MtgxR4395Btlr/Mmmh
Stock Number:
041684-MU
Citation ID:
RRID:MMRRC_041684-MU
Other Names:
R4395 (G1), C57BL/6J-MtgxR4395Btlr
Major Collection:

Strain Information

Tle4
Name: transducin-like enhancer of split 4
Synonyms: Grg4, ESTM13, ESTM14, Bce1, 5730411M05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 21888
VEGA: 19
Homologene: 38259
Slc4a7
Name: solute carrier family 4, sodium bicarbonate cotransporter, member 7
Synonyms: E430014N10Rik, NBC3, NBCn1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 218756
VEGA: 14
Homologene: 2680
Ndufv1
Name: NADH:ubiquinone oxidoreductase core subunit V1
Synonyms: 24 kDa (FP)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 17995
HGNC: HGNC:7716
Homologene: 5151
Stk17b
Name: serine/threonine kinase 17b (apoptosis-inducing)
Synonyms: Drak2, 3110009A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98267
Homologene: 31231
Tenm2
Name: teneurin transmembrane protein 2
Synonyms: Ten-m2, D3Bwg1534e, 9330187F13Rik, 2610040L17Rik, Odz2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 23964
Homologene: 22672
Rbm34
Name: RNA binding motif protein 34
Synonyms: 4930547K05Rik, D8Ertd233e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 52202
Homologene: 56691
Capza2
Name: capping actin protein of muscle Z-line subunit alpha 2
Synonyms: Cappa2, 1110053K06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 12343
HGNC: HGNC:1490
Homologene: 55956
Ubxn11
Name: UBX domain protein 11
Synonyms: 4930506L07Rik, Soci, Ubxd5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 67586
Homologene: 12159
Fam3b
Name: FAM3 metabolism regulating signaling molecule B
Synonyms: D16Jhu19e, 9030624C24Rik, ORF9, Pander
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 52793
HGNC: HGNC:1253
Homologene: 10766
Nxpe2
Name: neurexophilin and PC-esterase domain family, member 2
Synonyms: 4432416J03Rik, Fam55b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 78252
Homologene: 87072
Fat1
Name: FAT atypical cadherin 1
Synonyms: mFat1, 2310038E12Rik, Fath
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 14107
HGNC: HGNC:3595
Homologene: 66302
Homer3
Name: homer scaffolding protein 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 26558
Homologene: 37972
Trpm2
Name: transient receptor potential cation channel, subfamily M, member 2
Synonyms: Trrp7, LTRPC2, TRPC7, 9830168K16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 28240
Homologene: 20709
Tns2
Name: tensin 2
Synonyms: nep, nph, Tenc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 209039
VEGA: 15
Homologene: 37077
Lamc3
Name: laminin gamma 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 23928
HGNC: HGNC:6494
Homologene: 21222
Plekhs1
Name: pleckstrin homology domain containing, family S member 1
Synonyms: 9930023K05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 226245
VEGA: 19
Homologene: 11770
Tnxb
Name: tenascin XB
Synonyms: Tnx, TN-MHC
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 81877
Homologene: 49589
Gm9581
Name: predicted gene 9581
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Or5p51
Name: olfactory receptor family 5 subfamily P member 51
Synonyms: GA_x6K02T2PBJ9-10175273-10174329, MOR204-22, Olfr470
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258417
Map3k13
Name: mitogen-activated protein kinase kinase kinase 13
Synonyms: C130026N12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 71751
VEGA: 16
HGNC: HGNC:6852
Homologene: 37958
Alpk3
Name: alpha-kinase 3
Synonyms: Midori
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 116904
Homologene: 10813
Mtus2
Name: microtubule associated tumor suppressor candidate 2
Synonyms: 5730592G18Rik, A730013O20Rik, C130038G02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 77521
Homologene: 78141
Pik3cg
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma
Synonyms: PI3Kgamma, p110gamma, PI3Kgamma, PI(3)Kgamma, 5830428L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 30955
HGNC: HGNC:8978
Homologene: 68269
Grm8
Name: glutamate receptor, metabotropic 8
Synonyms: mGluR8, Gprc1h
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 14823
HGNC: HGNC:4600
Homologene: 654
Emilin1
Name: elastin microfibril interfacer 1
Synonyms: 5830419M17Rik, gp115, EMILIN-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100952
Homologene: 5117
Iqsec2
Name: IQ motif and Sec7 domain 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 245666
Homologene: 19407
Nod2
Name: nucleotide-binding oligomerization domain containing 2
Synonyms: F830032C23Rik, Nlrc2, Card15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 257632
HGNC: HGNC:5331
Homologene: 11156
Ltv1
Name: LTV1 ribosome biogenesis factor
Synonyms: 2610020N02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 353258
VEGA: 10
Homologene: 6338
Ints5
Name: integrator complex subunit 5
Synonyms: 1110055N21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 109077
Homologene: 35303
Acnat1
Name: acyl-coenzyme A amino acid N-acyltransferase 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230161
Homologene: 28287
Soga3
Name: SOGA family member 3
Synonyms: 6330407J23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 67412
VEGA: 10
Homologene: 28227
Adamtsl5
Name: ADAMTS-like 5
Synonyms: 2010109H09Rik, Thsd6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 66548
Homologene: 19803
Gm5724
Name: predicted gene 5724
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 435927
Or1s2
Name: olfactory receptor family 1 subfamily S member 1
Synonyms: GA_x6K02T2RE5P-4112771-4113718, MOR127-1, Olfr1496
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 258991
Homologene: 17458
Bahd1
Name: bromo adjacent homology domain containing 1
Synonyms: LOC228536
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228536
Homologene: 8976
Ttll8
Name: tubulin tyrosine ligase-like family, member 8
Synonyms: 1700019P01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 239591
Homologene: 52766
Chil4
Name: chitinase-like 4
Synonyms: Ym2, Chi3l4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 104183
Homologene: 74931
Ifit3b
Name: interferon-induced protein with tetratricopeptide repeats 3B
Synonyms: I830012O16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 667370
HGNC: HGNC:5411
Homologene: 1189
Ech1
Name: enoyl coenzyme A hydratase 1, peroxisomal
Synonyms: dienoyl-CoA isomerase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 51798
HGNC: HGNC:3149
Homologene: 1069
Crebl2
Name: cAMP responsive element binding protein-like 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232430
HGNC: HGNC:2350
Homologene: 1003
Maf1
Name: MAF1 homolog, negative regulator of RNA polymerase III
Synonyms: 1110068E11Rik, Maf1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 68877
Homologene: 49867
Adgrg4
Name: adhesion G protein-coupled receptor G4
Synonyms: LOC236798, PGR17, Gpr112
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 236798
Homologene: 72131
Cisd3
Name: CDGSH iron sulfur domain 3
Synonyms: Mel13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217149
Homologene: 35399
D10Bwg1379e
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Gm20528
Name: predicted gene 20528
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Hmga2
Name: high mobility group AT-hook 2
Synonyms: Hmgic, HMGI-C, 9430083A20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 15364
HGNC: HGNC:5009
Homologene: 2602
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 53,764,115 bp
  • CAGAGAGAGAGAGA to CAGAGAGAGAGA, chromosome 1 at 88,211,984 bp
  • A to G, chromosome 2 at 31,931,952 bp
  • G to A, chromosome 2 at 118,922,523 bp
  • G to A, chromosome 3 at 106,203,727 bp
  • A to G, chromosome 4 at 49,447,679 bp
  • G to T, chromosome 4 at 134,116,120 bp
  • T to C, chromosome 5 at 30,918,135 bp
  • A to T, chromosome 5 at 148,076,622 bp
  • T to C, chromosome 6 at 17,656,450 bp
  • T to A, chromosome 6 at 27,429,432 bp
  • A to G, chromosome 6 at 134,849,245 bp
  • T to C, chromosome 6 at 141,712,118 bp
  • T to C, chromosome 7 at 28,826,246 bp
  • C to T, chromosome 7 at 81,094,955 bp
  • A to G, chromosome 7 at 107,845,262 bp
  • T to A, chromosome 8 at 44,952,346 bp
  • G to A, chromosome 8 at 70,290,143 bp
  • T to A, chromosome 8 at 88,664,391 bp
  • T to C, chromosome 8 at 126,949,381 bp
  • A to G, chromosome 9 at 48,340,613 bp
  • T to C, chromosome 10 at 13,190,579 bp
  • A to G, chromosome 10 at 18,597,709 bp
  • T to C, chromosome 10 at 29,147,355 bp
  • T to C, chromosome 10 at 77,929,219 bp
  • A to G, chromosome 10 at 80,344,902 bp
  • C to T, chromosome 10 at 120,476,051 bp
  • C to T, chromosome 11 at 36,024,624 bp
  • A to G, chromosome 11 at 97,688,386 bp
  • T to C, chromosome 12 at 32,204,092 bp
  • C to T, chromosome 14 at 14,765,665 bp
  • T to C, chromosome 15 at 76,352,157 bp
  • C to T, chromosome 15 at 88,915,580 bp
  • C to T, chromosome 15 at 102,108,934 bp
  • A to T, chromosome 16 at 21,898,571 bp
  • A to G, chromosome 16 at 59,560,095 bp
  • C to A, chromosome 16 at 97,481,786 bp
  • C to T, chromosome 17 at 34,678,662 bp
  • T to A, chromosome 19 at 4,008,837 bp
  • A to G, chromosome 19 at 8,896,444 bp
  • A to T, chromosome 19 at 13,780,911 bp
  • A to T, chromosome 19 at 14,517,938 bp
  • G to T, chromosome 19 at 34,612,551 bp
  • A to G, chromosome 19 at 56,479,894 bp
  • T to G, chromosome X at 56,932,343 bp
  • C to T, chromosome X at 152,209,053 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4395 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041684-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.