Strain Name:
C57BL/6J-MtgxR4430Btlr/Mmmh
Stock Number:
041700-MU
Citation ID:
RRID:MMRRC_041700-MU
Other Names:
R4430 (G1), C57BL/6J-MtgxR4430Btlr
Major Collection:

Gene Information

Pax3
Name: paired box 3
Synonyms: Splchl2, Pax-3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 18505
HGNC: HGNC:8617
Homologene: 22494
Tert
Name: telomerase reverse transcriptase
Synonyms: TR
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 21752
VEGA: 13
Homologene: 31141
Gli2
Name: GLI-Kruppel family member GLI2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 14633
HGNC: HGNC:4318
Homologene: 12725
Mif4gd
Name: MIF4G domain containing
Synonyms: 2310075G12Rik, 1110014L05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 69674
Homologene: 41389
Bag3
Name: BCL2-associated athanogene 3
Synonyms: Bcl-2-interacting death suppressor, Bis
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 29810
HGNC: HGNC:939
Homologene: 3162
Mcm3
Name: minichromosome maintenance complex component 3
Synonyms: P1, p1.m, Mcmd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 17215
HGNC: HGNC:6945
Homologene: 1791
Rpap1
Name: RNA polymerase II associated protein 1
Synonyms: A730023M06Rik, 1190005L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 68925
Homologene: 32269
Ryr2
Name: ryanodine receptor 2, cardiac
Synonyms: 9330127I20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 20191
VEGA: 13
Homologene: 37423
Ahi1
Name: Abelson helper integration site 1
Synonyms: 1700015F03Rik, Ahi-1, Jouberin, D10Bwg0629e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 52906
VEGA: 10
Homologene: 9762
Cldn8
Name: claudin 8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Chemically Induced
NCBI: 54420
HGNC: HGNC:2050
Homologene: 8117
Zfp54
Name: zinc finger protein 54
Synonyms: clone 18, Zfp-54, KRAB10, Zfp76
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 22712
Homologene: 129791
Aak1
Name: AP2 associated kinase 1
Synonyms: D6Ertd245e, 5530400K14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 269774
Homologene: 128746
Tmem201
Name: transmembrane protein 201
Synonyms: Samp1, D4Ertd429e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 230917
Homologene: 35319
Mphosph9
Name: M-phase phosphoprotein 9
Synonyms: MPP9, MPP-9, B930097C17Rik, 9630025B04Rik, 4930548D04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 269702
HGNC: HGNC:7215
Homologene: 11256
L1td1
Name: LINE-1 type transposase domain containing 1
Synonyms: ECAT11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 381591
Homologene: 135709
Sost
Name: sclerostin
Synonyms: 5430411E23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 74499
Homologene: 11542
Trhde
Name: TRH-degrading enzyme
Synonyms: 9330155P21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 237553
Homologene: 75007
Dnah11
Name: dynein, axonemal, heavy chain 11
Synonyms: b2b1289Clo, b2b1727Clo, b2b598Clo, lrd, b2b1203Clo, b2b1279Clo, avc4, Dnahc11
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 13411
HGNC: HGNC:2942
Homologene: 2801
Stk10
Name: serine/threonine kinase 10
Synonyms: Lok, Gek1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 20868
Homologene: 38122
Pus7
Name: pseudouridylate synthase 7
Synonyms: C330017I15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 78697
Homologene: 6998
Ahnak
Name: AHNAK nucleoprotein (desmoyokin)
Synonyms: 1110004P15Rik, 2310047C17Rik, DY6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 66395
VEGA: 19
HGNC: HGNC:347
Homologene: 67425
Pglyrp3
Name: peptidoglycan recognition protein 3
Synonyms: LOC242100
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 242100
Homologene: 71559
Glis3
Name: GLIS family zinc finger 3
Synonyms: 4833409N03Rik, E330013K21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 226075
Homologene: 34989
Ugt2b37
Name: UDP glucuronosyltransferase 2 family, polypeptide B37
Synonyms: 0610033E06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 112417
Homologene: 137225
Vmn2r73
Name: vomeronasal 2, receptor 73
Synonyms: EG620928
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 620928
Homologene: 115466
Gm14412
Name: predicted gene 14412
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 102640477
Homologene: 134324
Ssc5d
Name: scavenger receptor cysteine rich family, 5 domains
Synonyms: A430110N23Rik, s5d-srcrb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 269855
Homologene: 77555
Col15a1
Name: collagen, type XV, alpha 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 12819
HGNC: HGNC:2192
Homologene: 1396
Pde3b
Name: phosphodiesterase 3B, cGMP-inhibited
Synonyms: 9830102A01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 18576
HGNC: HGNC:8779
Homologene: 709
Sox5
Name: SRY (sex determining region Y)-box 5
Synonyms: A730017D01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 20678
Homologene: 21378
0610040J01Rik
Name: RIKEN cDNA 0610040J01 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 76261
Homologene: 49537
Vmn2r22
Name: vomeronasal 2, receptor 22
Synonyms: EG546913
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 546913
Homologene: 84037
Tmem67
Name: transmembrane protein 67
Synonyms: 5330408M12Rik, b2b1163.1Clo, b2b1291.1Clo
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 329795
Homologene: 71886
Olfr1457
Name: olfactory receptor 1457
Synonyms: MOR202-20, GA_x6K02T2RE5P-3423041-3422097
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Chemically Induced
NCBI: 258568
Homologene: 133676
Pdzd3
Name: PDZ domain containing 3
Synonyms: sodium-phosphate cotransporter IIa C-terminal-associated protein 2, NaPi-Cap2, Pdzk2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 170761
VEGA: 9
Homologene: 11719
Ankrd55
Name: ankyrin repeat domain 55
Synonyms: C030011J08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 77318
VEGA: 13
Homologene: 12674
Sytl5
Name: synaptotagmin-like 5
Synonyms: ENSMUSG00000054453
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
Alteration at locus: Chemically Induced
NCBI: 236643
Homologene: 16345
Spata4
Name: spermatogenesis associated 4
Synonyms: SRG2, TSARG2, 1700001N01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 69281
Homologene: 15868
Cxcr2
Name: chemokine (C-X-C motif) receptor 2
Synonyms: IL8RA, CD128, Gpcr16, IL-8Rh, Cmkar2, Il8rb, IL-8rb
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 12765
HGNC: HGNC:6027
Homologene: 10439
C2cd6
Name: C2 calcium dependent domain containing 6
Synonyms: C2cd6b, Gm33589, Als2cr11, 4930408G06Rik, Als2cr11b, 1700052H20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 102636554
Homologene: 134310
Tmem181a
Name: transmembrane protein 181A
Synonyms: Gpr178, Tmem181, 5930418K15Rik, C76977
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 77106
Homologene: 44787
Olfr1079
Name: olfactory receptor 1079
Synonyms: GA_x6K02T2Q125-48024195-48023254, MOR189-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 258402
Homologene: 74090
Olfr806
Name: olfactory receptor 806
Synonyms: MOR110-12, MOR110-5, GA_x6K02T2PULF-11417610-11416669
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 258546
Homologene: 73936
Nim1k
Name: NIM1 serine/threonine protein kinase
Synonyms: Nim1, E130304F04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 245269
VEGA: 13
Homologene: 25286
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 20,811,993 bp
  • T to C, chromosome 1 at 59,004,142 bp
  • T to C, chromosome 1 at 74,158,845 bp
  • A to G, chromosome 1 at 78,195,324 bp
  • T to C, chromosome 1 at 118,837,244 bp
  • A to G, chromosome 2 at 86,538,387 bp
  • C to T, chromosome 2 at 119,771,067 bp
  • C to A, chromosome 2 at 177,315,832 bp
  • T to A, chromosome 3 at 92,031,491 bp
  • T to A, chromosome 4 at 12,051,473 bp
  • T to C, chromosome 4 at 47,245,705 bp
  • A to G, chromosome 4 at 98,737,151 bp
  • A to C, chromosome 4 at 149,731,139 bp
  • A to G, chromosome 5 at 23,746,489 bp
  • G to T, chromosome 5 at 63,898,839 bp
  • T to C, chromosome 5 at 87,254,092 bp
  • T to C, chromosome 5 at 124,265,446 bp
  • A to G, chromosome 6 at 86,986,366 bp
  • T to C, chromosome 6 at 123,637,858 bp
  • A to G, chromosome 6 at 144,041,274 bp
  • T to C, chromosome 7 at 4,943,664 bp
  • A to T, chromosome 7 at 85,870,241 bp
  • C to T, chromosome 7 at 114,534,670 bp
  • A to G, chromosome 7 at 128,523,923 bp
  • T to C, chromosome 8 at 54,601,843 bp
  • C to T, chromosome 9 at 44,249,744 bp
  • T to A, chromosome 10 at 20,972,078 bp
  • A to T, chromosome 10 at 114,503,123 bp
  • T to A, chromosome 10 at 129,738,261 bp
  • T to C, chromosome 11 at 32,533,552 bp
  • G to A, chromosome 11 at 101,966,844 bp
  • T to C, chromosome 11 at 115,608,502 bp
  • T to C, chromosome 12 at 117,983,011 bp
  • A to G, chromosome 13 at 11,735,527 bp
  • T to A, chromosome 13 at 73,627,475 bp
  • G to A, chromosome 13 at 112,323,183 bp
  • C to A, chromosome 13 at 119,712,542 bp
  • A to C, chromosome 16 at 88,562,731 bp
  • T to A, chromosome 17 at 6,295,786 bp
  • T to G, chromosome 17 at 21,434,960 bp
  • A to G, chromosome 19 at 9,003,040 bp
  • C to T, chromosome 19 at 13,095,088 bp
  • C to T, chromosome 19 at 28,262,905 bp
  • A to T, chromosome X at 9,960,023 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4430 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041700-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.