Strain Name:
C57BL/6J-MtgxR4449Btlr/Mmmh
Stock Number:
041710-MU
Citation ID:
RRID:MMRRC_041710-MU
Other Names:
R4449 (G1), C57BL/6J-MtgxR4449Btlr
Major Collection:

Gene Information

Sema3c
Name: sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C
Synonyms: 1110036B02Rik, Semae
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 20348
Homologene: 36201
Dap3
Name: death associated protein 3
Synonyms: DAP-3, 4921514D13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 65111
HGNC: HGNC:2673
Homologene: 3404
Ddc
Name: dopa decarboxylase
Synonyms: aromatic L-amino acid decarboxylase, Aadc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 13195
HGNC: HGNC:2719
Homologene: 618
Cadm1
Name: cell adhesion molecule 1
Synonyms: Igsf4, SynCam, RA175N, SgIGSF, RA175C, RA175B, RA175A, Igsf4a, Necl2, Tslc1, 2900073G06Rik, 3100001I08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 54725
HGNC: HGNC:5951
Homologene: 8641
Pitpnc1
Name: phosphatidylinositol transfer protein, cytoplasmic 1
Synonyms: 5830436L09Rik, C330017I21Rik, 1110020B03Rik, RDGB-BETA, RDGBB1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 71795
Homologene: 138301
Rngtt
Name: RNA guanylyltransferase and 5'-phosphatase
Synonyms: mouse capping enzyme
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 24018
Homologene: 37851
Luzp1
Name: leucine zipper protein 1
Synonyms: Luzp, 2700072H04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 269593
Homologene: 11545
Ubr1
Name: ubiquitin protein ligase E3 component n-recognin 1
Synonyms: E3 alpha
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 22222
Homologene: 7582
Hnrnpul1
Name: heterogeneous nuclear ribonucleoprotein U-like 1
Synonyms: Hnrnpul, E130317O14Rik, E1BAP5, E1B-AP5, Hnrpul1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 232989
Homologene: 31419
Helz
Name: helicase with zinc finger domain
Synonyms: 3110078M01Rik, 9630002H22Rik, 9430093I07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 78455
Homologene: 8918
Trappc12
Name: trafficking protein particle complex 12
Synonyms: D930014A20Rik, Ttc15, CGI-87
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 217449
VEGA: 12
Homologene: 34805
Bend3
Name: BEN domain containing 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 331623
VEGA: 10
Homologene: 19511
Unk
Name: unkempt family zinc finger
Synonyms: Zc3h5, B230379M23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217331
Homologene: 18194
Cntrob
Name: centrobin, centrosomal BRCA2 interacting protein
Synonyms: Nip2, Lip8, 9830165K03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 216846
Homologene: 14205
Twsg1
Name: twisted gastrulation BMP signaling modulator 1
Synonyms: D17Ertd403e, 9030422N06Rik, 1810013J15Rik, Tsg
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 65960
VEGA: 17
Homologene: 10774
Arfgap3
Name: ADP-ribosylation factor GTPase activating protein 3
Synonyms: 1810035F16Rik, 0610009H19Rik, 9130416J18Rik, 1810004P07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 66251
VEGA: 15
HGNC: HGNC:661
Homologene: 134103
Vps13b
Name: vacuolar protein sorting 13B
Synonyms: Coh1, C330002D13Rik, 2310042E16Rik, 1810042B05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 666173
VEGA: 15
HGNC: HGNC:2183
Homologene: 49516
Dpep2
Name: dipeptidase 2
Synonyms: F630103D06Rik, MBD-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 319446
Homologene: 49703
Pi4kb
Name: phosphatidylinositol 4-kinase beta
Synonyms: Pik4cb, ESTM41
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 107650
HGNC: HGNC:8984
Homologene: 6741
Arid3b
Name: AT rich interactive domain 3B (BRIGHT-like)
Synonyms: Dri2, Bdp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 56380
Homologene: 4721
Slc15a4
Name: solute carrier family 15, member 4
Synonyms: PTR4, PHT1, C130069N12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 100561
Homologene: 26432
Galnt2
Name: polypeptide N-acetylgalactosaminyltransferase 2
Synonyms: ppGaNTase-T2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 108148
HGNC: HGNC:4124
Homologene: 3297
Pcdh7
Name: protocadherin 7
Synonyms: BH-protocadherin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 54216
HGNC: HGNC:8659
Homologene: 36101
Wdfy4
Name: WD repeat and FYVE domain containing 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 545030
Homologene: 83473
Mlycd
Name: malonyl-CoA decarboxylase
Synonyms: Mcd
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 56690
HGNC: HGNC:7150
Homologene: 40821
Ttc39a
Name: tetratricopeptide repeat domain 39A
Synonyms: 4922503N01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230603
Homologene: 17739
Tdrd6
Name: tudor domain containing 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 210510
Homologene: 19364
Skint3
Name: selection and upkeep of intraepithelial T cells 3
Synonyms: A430090E18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 195564
Slc17a3
Name: solute carrier family 17 (sodium phosphate), member 3
Synonyms: Npt4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 105355
Homologene: 21319
Ubqlnl
Name: ubiquilin-like
Synonyms: LOC244179, 4922504M18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 244179
Homologene: 17034
Kcnh6
Name: potassium voltage-gated channel, subfamily H (eag-related), member 6
Synonyms: m-erg2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 192775
Homologene: 32740
Prpf40b
Name: pre-mRNA processing factor 40B
Synonyms: 2610317D23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 54614
Homologene: 81828
Trim34a
Name: tripartite motif-containing 34A
Synonyms: Trim34, Trim34-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 94094
Homologene: 128238
Olfr593
Name: olfactory receptor 593
Synonyms: GA_x6K02T2PBJ9-5927412-5928362, MOR24-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258378
Homologene: 27147
Gm10722
Name: predicted gene 10722
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Bpifb6
Name: BPI fold containing family B, member 6
Synonyms: LOC228796, Bpil3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 228796
Homologene: 18375
Snx14
Name: sorting nexin 14
Synonyms: C330035N22Rik, YR-14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 244962
Homologene: 17823
Hsdl2
Name: hydroxysteroid dehydrogenase like 2
Synonyms: 2610207I16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 72479
Fut10
Name: fucosyltransferase 10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 171167
Homologene: 32790
Sgce
Name: sarcoglycan, epsilon
Synonyms: e-SG
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 20392
Homologene: 31205
Shisa6
Name: shisa family member 6
Synonyms: LOC380702, Gm879, CKAMP52
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380702
Homologene: 66164
Zcchc3
Name: zinc finger, CCHC domain containing 3
Synonyms: 2810406K24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 67917
Homologene: 13214
Myl7
Name: myosin, light polypeptide 7, regulatory
Synonyms: RLC-A, MLC-2alpha, Mylc2a, MLC2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 17898
Homologene: 23290
Igkv3-2
Name: immunoglobulin kappa variable 3-2
Synonyms: V(kappa)21A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 626583
Gm11831
Name: predicted gene 11831
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 2 at 120,946,381 bp
  • G to C, chromosome 2 at 152,414,722 bp
  • A to G, chromosome 2 at 153,906,768 bp
  • A to T, chromosome 3 at 88,949,878 bp
  • C to T, chromosome 3 at 94,984,735 bp
  • T to C, chromosome 4 at 11,498,828 bp
  • T to C, chromosome 4 at 33,330,865 bp
  • T to A, chromosome 4 at 59,617,692 bp
  • T to C, chromosome 4 at 109,442,303 bp
  • T to A, chromosome 4 at 112,270,009 bp
  • T to A, chromosome 4 at 136,540,863 bp
  • A to G, chromosome 5 at 17,576,846 bp
  • A to G, chromosome 5 at 57,720,485 bp
  • A to G, chromosome 5 at 127,604,536 bp
  • A to G, chromosome 6 at 4,717,930 bp
  • G to A, chromosome 6 at 70,698,841 bp
  • T to C, chromosome 7 at 25,722,284 bp
  • A to T, chromosome 7 at 103,212,480 bp
  • C to T, chromosome 7 at 104,149,718 bp
  • T to C, chromosome 7 at 104,335,728 bp
  • T to A, chromosome 8 at 31,236,257 bp
  • T to C, chromosome 8 at 105,988,841 bp
  • T to A, chromosome 8 at 119,410,405 bp
  • A to G, chromosome 8 at 124,295,377 bp
  • A to C, chromosome 9 at 3,001,041 bp
  • C to T, chromosome 9 at 47,530,437 bp
  • T to A, chromosome 9 at 47,813,988 bp
  • T to A, chromosome 9 at 57,798,121 bp
  • T to C, chromosome 9 at 88,422,999 bp
  • A to G, chromosome 10 at 43,512,083 bp
  • C to T, chromosome 11 at 5,897,354 bp
  • T to C, chromosome 11 at 11,835,802 bp
  • T to C, chromosome 11 at 66,525,418 bp
  • C to A, chromosome 11 at 69,305,549 bp
  • A to G, chromosome 11 at 106,018,936 bp
  • A to G, chromosome 11 at 107,216,709 bp
  • T to C, chromosome 11 at 107,604,163 bp
  • G to A, chromosome 11 at 116,053,634 bp
  • A to T, chromosome 12 at 28,747,235 bp
  • A to T, chromosome 13 at 23,856,732 bp
  • T to A, chromosome 14 at 33,096,083 bp
  • T to C, chromosome 15 at 35,876,793 bp
  • A to T, chromosome 15 at 83,334,558 bp
  • A to G, chromosome 15 at 99,314,663 bp
  • C to T, chromosome 17 at 43,629,735 bp
  • A to C, chromosome 17 at 65,926,310 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4449 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041710-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.