Strain Name:
C57BL/6J-MtgxR4456Btlr/Mmmh
Stock Number:
041716-MU
Citation ID:
RRID:MMRRC_041716-MU
Other Names:
R4456 (G1), C57BL/6J-MtgxR4456Btlr
Major Collection:

Gene Information

Kmt2c
Name: lysine (K)-specific methyltransferase 2C
Synonyms: E330008K23Rik, HALR, Mll3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 231051
Homologene: 46480
Cdkn2d
Name: cyclin dependent kinase inhibitor 2D
Synonyms: p19, INK4d, p19INK4d, INK4d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 12581
VEGA: 9
HGNC: HGNC:1790
Homologene: 36081
Eya1
Name: EYA transcriptional coactivator and phosphatase 1
Synonyms: bor
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 14048
HGNC: HGNC:3519
Homologene: 74943
Dst
Name: dystonin
Synonyms: A830042E19Rik, 2310001O04Rik, Macf2, bullous pemphigoid antigen 1, BPAG1-n, BPAG1, athetoid, Bpag, ah, bullous pemphigoid antigen 1, Bpag1, nmf339, nmf203
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Dnmt1
Name: DNA methyltransferase (cytosine-5) 1
Synonyms: MTase, Cxxc9, MommeD2, Dnmt1o
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 13433
VEGA: 9
HGNC: HGNC:2976
Homologene: 124071
Nbea
Name: neurobeachin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 26422
HGNC: HGNC:7648
Homologene: 69190
Shroom3
Name: shroom family member 3
Synonyms: Shrm3, Shrm, D5Ertd287e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 27428
Homologene: 9263
Tom1l2
Name: target of myb1-like 2 (chicken)
Synonyms: 2900016I08Rik, A730055F12Rik, myb1-like protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 216810
Homologene: 44901
Fbxo38
Name: F-box protein 38
Synonyms: 6030410I24Rik, SP329
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 107035
VEGA: 18
Homologene: 34526
Tbc1d5
Name: TBC1 domain family, member 5
Synonyms: 1600014N05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 72238
VEGA: 17
Homologene: 8834
Atg4c
Name: autophagy related 4C, cysteine peptidase
Synonyms: Apg4-C, autophagin 3, Apg4c, Autl1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 242557
Homologene: 56296
Fcer1g
Name: Fc receptor, IgE, high affinity I, gamma polypeptide
Synonyms: Ly-50, Fce1g, FcepsilonRI, FcR[g], FcR-gamma, FcRgamma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 14127
HGNC: HGNC:3611
Homologene: 3029
Apob
Name: apolipoprotein B
Synonyms: apob-48, apob-100
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
Alteration at locus: Chemically Induced
NCBI: 238055
HGNC: HGNC:603
Homologene: 328
Rnf4
Name: ring finger protein 4
Synonyms: Gtrgeo8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 19822
Homologene: 37711
Mkln1
Name: muskelin 1, intracellular mediator containing kelch motifs
Synonyms: A130067F06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
Alteration at locus: Chemically Induced
NCBI: 27418
HGNC: HGNC:7109
Homologene: 8305
Hectd4
Name: HECT domain E3 ubiquitin protein ligase 4
Synonyms: Gm15800
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 269700
Homologene: 28297
Amfr
Name: autocrine motility factor receptor
Synonyms: gp78
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 23802
HGNC: HGNC:463
Homologene: 888
Mslnl
Name: mesothelin-like
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 328783
VEGA: 17
HGNC: null
Homologene: 18633
Loxhd1
Name: lipoxygenase homology domains 1
Synonyms: 1700096C21Rik, sba
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Chemically Induced
NCBI: 240411
Homologene: null
Naip2
Name: NLR family, apoptosis inhibitory protein 2
Synonyms: Naip2, Birc1b, Naip-rs6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 17948
HGNC: HGNC:7634
Homologene: 136092
Mroh2b
Name: maestro heat-like repeat family member 2B
Synonyms: 4930455B06Rik, Heatr7b2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 223825
VEGA: 15
Homologene: 128807
Nckap5
Name: NCK-associated protein 5
Synonyms: E030049G20Rik, D130011D22Rik, LOC380609
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
Alteration at locus: Chemically Induced
NCBI: 210356
Homologene: 35542
Tbc1d17
Name: TBC1 domain family, member 17
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 233204
Homologene: 11656
Abca8b
Name: ATP-binding cassette, sub-family A (ABC1), member 8b
Synonyms: Abca8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 27404
HGNC: HGNC:38
Homologene: 56029
Mthfsd
Name: methenyltetrahydrofolate synthetase domain containing
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 234814
Homologene: 11245
Emcn
Name: endomucin
Synonyms: 0610012K22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 59308
Homologene: 9438
Notch4
Name: notch 4
Synonyms: Int-3, Int3, N4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 18132
HGNC: HGNC:7884
Homologene: 3351
Glp1r
Name: glucagon-like peptide 1 receptor
Synonyms: GLP-1R, GLP1Rc
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 14652
VEGA: 17
HGNC: HGNC:4324
Homologene: 1558
Ttc28
Name: tetratricopeptide repeat domain 28
Synonyms: 2310015L07Rik, TPRBK
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Chemically Induced
NCBI: 209683
Homologene: 41023
Olfr643
Name: olfactory receptor 643
Synonyms: MOR13-2, GA_x6K02T2PBJ9-6793628-6792684
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Chemically Induced
NCBI: 259081
HGNC: null
Homologene: 17491
Hsd3b1
Name: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
Synonyms: D3Ertd383e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Alteration at locus: Chemically Induced
NCBI: 15492
Homologene: 69149
Slc2a4
Name: solute carrier family 2 (facilitated glucose transporter), member 4
Synonyms: Glut4, twgy, Glut-4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Chemically Induced
NCBI: 20528
Homologene: 74381
Txndc15
Name: thioredoxin domain containing 15
Synonyms: 2310047H23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Chemically Induced
NCBI: 69672
VEGA: 13
Homologene: 32594
Tcp11l1
Name: t-complex 11 like 1
Synonyms: C130096D04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 320554
Homologene: 41260
Gnmt
Name: glycine N-methyltransferase
Synonyms: glycine N methyl transferase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Chemically Induced
NCBI: 14711
HGNC: HGNC:4415
Homologene: 7741
Rasl12
Name: RAS-like, family 12
Synonyms: 4631404I11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Chemically Induced
NCBI: 70784
VEGA: 9
Homologene: 9565
Bahd1
Name: bromo adjacent homology domain containing 1
Synonyms: LOC228536
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Chemically Induced
NCBI: 228536
Homologene: 8976
Mri1
Name: methylthioribose-1-phosphate isomerase 1
Synonyms: 2410018C20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: Chemically Induced
NCBI: 67873
Homologene: 7160
Rab36
Name: RAB36, member RAS oncogene family
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Chemically Induced
NCBI: 76877
HGNC: HGNC:9775
Homologene: 3610
Cdc42ep1
Name: CDC42 effector protein (Rho GTPase binding) 1
Synonyms: 1810058K22Rik, MSE55, CEP1, Borg5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Chemically Induced
NCBI: 104445
VEGA: 15
Homologene: 5128
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • C to T, chromosome 1 at 14,183,196 bp
  • A to T, chromosome 1 at 34,190,719 bp
  • A to T, chromosome 1 at 125,914,735 bp
  • A to G, chromosome 1 at 171,234,239 bp
  • A to T, chromosome 2 at 104,684,222 bp
  • C to T, chromosome 2 at 118,916,406 bp
  • A to G, chromosome 3 at 55,643,784 bp
  • G to A, chromosome 3 at 98,856,143 bp
  • A to T, chromosome 3 at 137,379,847 bp
  • A to G, chromosome 4 at 99,221,304 bp
  • A to T, chromosome 5 at 25,310,212 bp
  • A to G, chromosome 5 at 34,351,361 bp
  • A to G, chromosome 5 at 92,940,999 bp
  • AC to A, chromosome 5 at 111,224,058 bp
  • C to T, chromosome 5 at 121,308,271 bp
  • A to G, chromosome 6 at 31,426,772 bp
  • A to G, chromosome 7 at 44,841,312 bp
  • A to G, chromosome 7 at 104,059,300 bp
  • C to A, chromosome 8 at 84,256,406 bp
  • C to T, chromosome 8 at 93,984,940 bp
  • C to T, chromosome 8 at 121,105,765 bp
  • T to C, chromosome 9 at 20,909,842 bp
  • C to G, chromosome 9 at 21,290,889 bp
  • A to G, chromosome 9 at 65,398,584 bp
  • G to A, chromosome 10 at 75,044,496 bp
  • G to T, chromosome 11 at 60,352,815 bp
  • G to A, chromosome 11 at 69,943,322 bp
  • A to G, chromosome 11 at 109,942,245 bp
  • T to A, chromosome 12 at 8,015,445 bp
  • A to G, chromosome 13 at 55,718,164 bp
  • T to A, chromosome 13 at 100,154,911 bp
  • A to T, chromosome 15 at 4,947,925 bp
  • A to G, chromosome 15 at 78,849,891 bp
  • G to A, chromosome 17 at 25,742,934 bp
  • G to T, chromosome 17 at 30,918,975 bp
  • G to A, chromosome 17 at 34,583,833 bp
  • G to T, chromosome 17 at 46,728,984 bp
  • A to C, chromosome 17 at 50,782,341 bp
  • G to T, chromosome 18 at 62,526,249 bp
  • A to G, chromosome 18 at 77,399,089 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4456 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041716-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.