Strain Name:
C57BL/6J-MtgxR4468Btlr/Mmmh
Stock Number:
041725-MU
Citation ID:
RRID:MMRRC_041725-MU
Other Names:
R4468 (G1), C57BL/6J-MtgxR4468Btlr
Major Collection:

Gene Information

Ppp2r3c
Name: protein phosphatase 2, regulatory subunit B'', gamma
Synonyms: 5730412A08Rik, G4-1, G5pr, 4930511A21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 59032
VEGA: 12
Homologene: 9915
Adam23
Name: a disintegrin and metallopeptidase domain 23
Synonyms: MDC3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 23792
HGNC: HGNC:202
Homologene: 2826
Chd1
Name: chromodomain helicase DNA binding protein 1
Synonyms: 4930525N21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 12648
HGNC: HGNC:1915
Homologene: 68174
Skp1
Name: S-phase kinase-associated protein 1
Synonyms: 2610206H23Rik, 2610043E24Rik, p19Skp1, Tceb1l, Skp1a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 21402
Homologene: 38775
Wls
Name: wntless WNT ligand secretion mediator
Synonyms: 5031439A09Rik, Gpr177
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 68151
Homologene: 11779
Riox2
Name: ribosomal oxygenase 2
Synonyms: 1810047J07Rik, 3830408E23Rik, 2410057H13Rik, Mina
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 67014
Homologene: 12071
Sos1
Name: SOS Ras/Rac guanine nucleotide exchange factor 1
Synonyms: 4430401P03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 20662
VEGA: 17
Homologene: 4117
Bmp2
Name: bone morphogenetic protein 2
Synonyms: Bmp2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12156
HGNC: HGNC:1069
Homologene: 926
Snx29
Name: sorting nexin 29
Synonyms: LOC381035, LOC385605, 4933437K13Rik, Gm11170, Rundc2a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 74478
Homologene: 32706
Shoc2
Name: Shoc2, leucine rich repeat scaffold protein
Synonyms: Sur-8, soc-2 (suppressor of clear) homolog (C. elegans)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 56392
VEGA: 19
Homologene: 7219
Kirrel
Name: kirre like nephrin family adhesion molecule 1
Synonyms: Neph1, Kirrel1, 6720469N11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 170643
Homologene: 10089
Pwwp3a
Name: PWWP domain containing 3A, DNA repair factor
Synonyms: 9430059D04Rik, Mum1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 68114
Homologene: 11349
Phf10
Name: PHD finger protein 10
Synonyms: 1810055P05Rik, Baf45a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 72057
Homologene: 10112
Clec4f
Name: C-type lectin domain family 4, member f
Synonyms: kupffer cell receptor, D18063, Clecsf13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 51811
Homologene: 9630
Txndc11
Name: thioredoxin domain containing 11
Synonyms: EF-hand binding protein 1, EFP1, Txdc11, 2810408E11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 106200
Homologene: 9301
Adgrv1
Name: adhesion G protein-coupled receptor V1
Synonyms: VLGR1, Mass1, Mgr1, Gpr98
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 110789
Homologene: 19815
Ifit1bl2
Name: interferon induced protein with tetratricopeptide repeats 1B like 2
Synonyms: 2010002M12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 112419
Homologene: 78213
Ros1
Name: Ros1 proto-oncogene
Synonyms: Ros-1, c-ros
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 19886
Homologene: 2207
Kcnh2
Name: potassium voltage-gated channel, subfamily H (eag-related), member 2
Synonyms: merg1a, ether a go-go related, M-erg, LQT, Lqt2, ERG1, merg1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 16511
HGNC: HGNC:6251
Homologene: 201
Tns4
Name: tensin 4
Synonyms: 9930017A07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 217169
Homologene: 13147
Spag17
Name: sperm associated antigen 17
Synonyms: 4931427F14Rik, PF6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 74362
Homologene: 52601
Abca2
Name: ATP-binding cassette, sub-family A (ABC1), member 2
Synonyms: Abc2, D2H0S1474E
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 11305
HGNC: HGNC:32
Homologene: 55590
Olfr155
Name: olfactory receptor 155
Synonyms: mOR37a, OR37A, Olfr37a, GA_x6K02T2N78B-16092200-16091241, MOR262-14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 29845
HGNC: HGNC:8276
Homologene: 84331
Ccdc33
Name: coiled-coil domain containing 33
Synonyms: LOC382077, 4930535E21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 382077
Homologene: 19641
Scn11a
Name: sodium channel, voltage-gated, type XI, alpha
Synonyms: SNS2, NaN, NaT, NSS2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 24046
VEGA: 9
Homologene: 8041
Lancl2
Name: LanC (bacterial lantibiotic synthetase component C)-like 2
Synonyms: 1700003F10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 71835
HGNC: HGNC:6509
Homologene: 23116
Il12a
Name: interleukin 12a
Synonyms: IL-12p35, p35
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 16159
HGNC: HGNC:5969
Homologene: 681
Rad9a
Name: RAD9 checkpoint clamp component A
Synonyms: Rad9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 19367
VEGA: 19
HGNC: HGNC:9827
Homologene: 32118
Aanat
Name: arylalkylamine N-acetyltransferase
Synonyms: Nat-2, Nat4, SNAT
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 11298
HGNC: HGNC:19
Homologene: 31013
Prxl2b
Name: peroxiredoxin like 2B
Synonyms: PM/PGFS, 2810405K02Rik, Fam213b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 66469
Homologene: 11976
Olfr847
Name: olfactory receptor 847
Synonyms: GA_x6K02T2PVTD-13113073-13112135, MOR149-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 258518
Homologene: 17281
Ttll2
Name: tubulin tyrosine ligase-like family, member 2
Synonyms: EG625850
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 100216474
VEGA: 17
Homologene: 12917
Gm17690
Name: predicted gene, 17690
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
Rps6-ps2
Name: ribosomal protein S6, pseudogene 2
Synonyms: Gm16406
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 675985
Igkv6-15
Name: immunoglobulin kappa variable 6-15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 108022
Rps12-ps24
Name: ribosomal protein S12, pseudogene 24
Synonyms: Gm10063, Rps12-ps24, Rps12l2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 671641
Homologene: 138738
Olfr104-ps
Name: olfactory receptor 104, pseudogene
Synonyms: MOR250-5, GA_x6K02T2PSCP-1823235-1824161, Olfr104
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 257948
Pcdha5
Name: protocadherin alpha 5
Synonyms: Cnr6, Crnr6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 12941
HGNC: HGNC:8671
Homologene: 49565
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 63,570,967 bp
  • A to G, chromosome 2 at 25,444,902 bp
  • T to C, chromosome 2 at 133,554,454 bp
  • T to A, chromosome 3 at 68,697,936 bp
  • C to T, chromosome 3 at 87,089,151 bp
  • T to C, chromosome 3 at 96,167,891 bp
  • A to T, chromosome 3 at 100,085,366 bp
  • C to A, chromosome 3 at 159,872,927 bp
  • G to A, chromosome 4 at 43,854,737 bp
  • T to G, chromosome 4 at 154,897,050 bp
  • G to A, chromosome 5 at 24,325,591 bp
  • T to A, chromosome 6 at 57,713,034 bp
  • A to G, chromosome 6 at 70,406,973 bp
  • A to G, chromosome 6 at 83,652,433 bp
  • A to G, chromosome 8 at 36,026,114 bp
  • A to G, chromosome 8 at 88,806,691 bp
  • C to T, chromosome 9 at 19,375,648 bp
  • A to G, chromosome 9 at 58,029,952 bp
  • G to T, chromosome 9 at 58,069,872 bp
  • G to A, chromosome 9 at 119,754,987 bp
  • T to A, chromosome 10 at 52,118,356 bp
  • C to T, chromosome 10 at 80,240,736 bp
  • C to T, chromosome 11 at 52,245,078 bp
  • A to T, chromosome 11 at 99,070,415 bp
  • A to T, chromosome 11 at 116,596,955 bp
  • C to T, chromosome 12 at 55,297,883 bp
  • T to A, chromosome 13 at 81,374,256 bp
  • T to C, chromosome 16 at 11,075,223 bp
  • T to A, chromosome 16 at 11,420,701 bp
  • T to A, chromosome 16 at 59,475,994 bp
  • T to C, chromosome 17 at 7,352,435 bp
  • T to C, chromosome 17 at 14,952,775 bp
  • T to C, chromosome 17 at 15,760,395 bp
  • A to G, chromosome 17 at 37,362,637 bp
  • A to G, chromosome 17 at 80,453,811 bp
  • T to C, chromosome 18 at 36,962,180 bp
  • G to A, chromosome 19 at 4,200,294 bp
  • G to A, chromosome 19 at 34,619,068 bp
  • A to G, chromosome 19 at 54,026,414 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4468 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041725-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.