Strain Name:
C57BL/6J-MtgxR4497Btlr/Mmmh
Stock Number:
041750-MU
Citation ID:
RRID:MMRRC_041750-MU
Other Names:
R4497 (G1), C57BL/6J-MtgxR4497Btlr
Major Collection:

Gene Information

Trmo
Name: tRNA methyltransferase O
Synonyms: 5830415F09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 74753
Homologene: 32316
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Birc2
Name: baculoviral IAP repeat-containing 2
Synonyms: mcIAP1, MIAP1, IAP1, MIHB, Api1, cIAP1, cIAP-1, HIAP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 11797
HGNC: HGNC:590
Homologene: 900
Fbxl3
Name: F-box and leucine-rich repeat protein 3
Synonyms: Fbl3a, Fbxl3a, Ovtm, Play68
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 50789
Homologene: 8127
Aco2
Name: aconitase 2, mitochondrial
Synonyms: Aco3, Aco-2, D10Wsu183e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 11429
HGNC: HGNC:118
Homologene: 856
Arhgap12
Name: Rho GTPase activating protein 12
Synonyms: 2810011M08Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 75415
Homologene: 23089
Stk11ip
Name: serine/threonine kinase 11 interacting protein
Synonyms: LKB1IP, LIP1, 1200014D22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 71728
Homologene: 12406
Rorb
Name: RAR-related orphan receptor beta
Synonyms: RZR-beta, Nr1f2, Rorbeta, hstp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 225998
Homologene: 38250
Itprid2
Name: ITPR interacting domain containing 2
Synonyms: SPAG13, CS-1, CS1, KRAP, Ssfa2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 70599
Homologene: 4912
Wdr33
Name: WD repeat domain 33
Synonyms: 2810021O11Rik, 8430413N20Rik, 2310011G05Rik, 1110001N06Rik, WDC146
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 74320
VEGA: 18
Homologene: 56807
Lamb2
Name: laminin, beta 2
Synonyms: Lamb-2, Lams, npht
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 16779
HGNC: HGNC:6487
Homologene: 1723
Ccdc92
Name: coiled-coil domain containing 92
Synonyms: D5Bwg0834e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 215707
Homologene: 11848
Adat1
Name: adenosine deaminase, tRNA-specific 1
Synonyms: mADAT1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 30947
HGNC: HGNC:228
Homologene: 8096
Pgr
Name: progesterone receptor
Synonyms: NR3C3, PR, 9930019P03Rik, PR-B, PR-A, ENSMUSG00000074510
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 18667
HGNC: HGNC:8910
Homologene: 713
Zbtb18
Name: zinc finger and BTB domain containing 18
Synonyms: RP58, Zfp238
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 30928
Homologene: 21276
Reck
Name: reversion-inducing-cysteine-rich protein with kazal motifs
Synonyms: St15
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 53614
Homologene: 9622
Zfp81
Name: zinc finger protein 81
Synonyms: KRAB13, Zfp78, Hszfp36, C330034P10Rik, D330034E10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224694
Homologene: 138633
Eml2
Name: echinoderm microtubule associated protein like 2
Synonyms: 1600029N02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 72205
Homologene: 8125
Zfhx4
Name: zinc finger homeodomain 4
Synonyms: Zfh-4, C130041O22Rik, Zfh4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 80892
Homologene: 23477
4932431P20Rik
Name: RIKEN cDNA 4932431P20 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 114675
Astn2
Name: astrotactin 2
Synonyms: 1d8, Astnl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 56079
Homologene: 77850
Man2b1
Name: mannosidase 2, alpha B1
Synonyms: lysosomal alpha-mannosidase
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 17159
HGNC: HGNC:6826
Homologene: 37322
Rab44
Name: RAB44, member RAS oncogene family
Synonyms: 9830134C10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 442827
Homologene: 53300
Tprgl
Name: transformation related protein 63 regulated like
Synonyms: 1200015A19Rik, mover
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 67808
Homologene: 12192
Slx4
Name: SLX4 structure-specific endonuclease subunit homolog (S. cerevisiae)
Synonyms: D16Bwg1016e, Btbd12
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 52864
Homologene: 23770
Myo7b
Name: myosin VIIB
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 17922
HGNC: HGNC:7607
Homologene: 81947
Gabrb2
Name: gamma-aminobutyric acid (GABA) A receptor, subunit beta 2
Synonyms: Gabrb-2, C030021G16Rik, C030002O17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 14401
HGNC: HGNC:4082
Homologene: 7327
Spag16
Name: sperm associated antigen 16
Synonyms: Pf20, 4930524F24Rik, 4930585K05Rik, Wdr29, 4921511D23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 66722
Homologene: 11572
Ttyh2
Name: tweety family member 2
Synonyms: 1110001A03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 117160
Homologene: 41882
Cps1
Name: carbamoyl-phosphate synthetase 1
Synonyms: CPS, CPSase I, 4732433M03Rik, D1Ucla3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 227231
HGNC: HGNC:2323
Homologene: 68208
Gli3
Name: GLI-Kruppel family member GLI3
Synonyms: Bph, brachyphalangy
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 14634
HGNC: HGNC:4319
Homologene: 139
Lacc1
Name: laccase domain containing 1
Synonyms: 9030625A04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 210808
Homologene: 17712
Tmf1
Name: TATA element modulatory factor 1
Synonyms: LOC232286, 7030402D04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 232286
Homologene: 133801
Fhod3
Name: formin homology 2 domain containing 3
Synonyms: A930009H06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225288
VEGA: 18
Homologene: 45323
Bcas1
Name: breast carcinoma amplified sequence 1
Synonyms: NABC1, 2210416M21Rik, 9030223A09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 76960
HGNC: HGNC:974
Homologene: 2714
Slc23a2
Name: solute carrier family 23 (nucleobase transporters), member 2
Synonyms: YSPL3, SVCT2, Slc23a1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 54338
Homologene: 68440
Serinc2
Name: serine incorporator 2
Synonyms: FKSG84, TDE2, 2310004K20Rik, Tde2l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 230779
Homologene: 27797
Ppp1r3g
Name: protein phosphatase 1, regulatory subunit 3G
Synonyms: 1600032L17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 76487
Homologene: 109865
Aoc3
Name: amine oxidase, copper containing 3
Synonyms: SSAO, semicarbazide-sensitive amine oxidase, VAP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 11754
HGNC: HGNC:550
Homologene: 2770
Mmp24
Name: matrix metallopeptidase 24
Synonyms: MT5-MMP, Membrane type 5-MMP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 17391
HGNC: HGNC:7172
Homologene: 21331
Apobr
Name: apolipoprotein B receptor
Synonyms: Apob-48r, Apob48r
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 171504
Homologene: 26695
Olfr497
Name: olfactory receptor 497
Synonyms: GA_x6K02T2PBJ9-10752603-10753547, MOR204-9
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 258733
Homologene: 133602
Spopl
Name: speckle-type BTB/POZ protein-like
Synonyms: E430033K04Rik, 4921517N04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 76857
Homologene: 78016
Cacnb4
Name: calcium channel, voltage-dependent, beta 4 subunit
Synonyms: Cchb4, 3110038O15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12298
HGNC: HGNC:1404
Homologene: 20188
Cbx8
Name: chromobox 8
Synonyms: polycomb 3, Pc3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 30951
Homologene: 32199
Wdr55
Name: WD repeat domain 55
Synonyms: 2410080P20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 67936
VEGA: 18
Homologene: 9789
Tssk5
Name: testis-specific serine kinase 5
Synonyms: 1700091F14Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 73542
VEGA: 15
Homologene: 18832
Olfr156
Name: olfactory receptor 156
Synonyms: mOR37b, OR37B, Olfr37b, MOR262-6, GA_x6K02T2N78B-16125144-16126100
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 29846
Homologene: 133652
Gm10420
Name: predicted gene 10420
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 108169197
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • G to T, chromosome 1 at 67,205,199 bp
  • A to G, chromosome 1 at 70,493,830 bp
  • C to T, chromosome 1 at 75,527,139 bp
  • A to T, chromosome 1 at 177,447,121 bp
  • C to T, chromosome 2 at 23,517,945 bp
  • T to A, chromosome 2 at 52,477,771 bp
  • T to A, chromosome 2 at 79,657,820 bp
  • G to A, chromosome 2 at 132,056,782 bp
  • A to G, chromosome 2 at 155,813,988 bp
  • T to C, chromosome 2 at 170,406,821 bp
  • G to A, chromosome 3 at 5,399,620 bp
  • A to G, chromosome 4 at 43,821,175 bp
  • G to A, chromosome 4 at 43,891,001 bp
  • C to T, chromosome 4 at 46,382,140 bp
  • A to G, chromosome 4 at 66,119,063 bp
  • G to T, chromosome 4 at 130,254,054 bp
  • G to A, chromosome 4 at 154,159,323 bp
  • G to A, chromosome 5 at 124,836,273 bp
  • A to T, chromosome 6 at 97,172,332 bp
  • A to T, chromosome 6 at 122,696,223 bp
  • G to A, chromosome 7 at 19,179,350 bp
  • C to A, chromosome 7 at 29,537,738 bp
  • T to A, chromosome 7 at 108,422,915 bp
  • A to T, chromosome 7 at 126,587,522 bp
  • G to A, chromosome 8 at 85,090,936 bp
  • T to C, chromosome 8 at 111,979,362 bp
  • A to G, chromosome 9 at 7,836,900 bp
  • A to G, chromosome 9 at 8,958,419 bp
  • T to A, chromosome 9 at 108,486,798 bp
  • T to C, chromosome 11 at 42,597,694 bp
  • T to G, chromosome 11 at 101,332,045 bp
  • A to T, chromosome 11 at 114,710,963 bp
  • C to A, chromosome 11 at 119,040,792 bp
  • T to A, chromosome 13 at 15,723,571 bp
  • T to A, chromosome 13 at 35,969,620 bp
  • T to A, chromosome 14 at 77,034,030 bp
  • G to A, chromosome 14 at 103,082,877 bp
  • T to C, chromosome 15 at 76,372,211 bp
  • G to A, chromosome 15 at 81,895,285 bp
  • T to C, chromosome 16 at 3,994,909 bp
  • T to A, chromosome 16 at 15,700,653 bp
  • G to A, chromosome 17 at 29,139,897 bp
  • A to T, chromosome 17 at 33,334,703 bp
  • A to T, chromosome 18 at 6,111,774 bp
  • T to A, chromosome 18 at 25,110,239 bp
  • A to C, chromosome 18 at 31,893,079 bp
  • A to G, chromosome 18 at 32,014,229 bp
  • G to A, chromosome 18 at 36,760,395 bp
  • G to T, chromosome 19 at 18,977,628 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4497 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
041750-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.