Strain Name:
C57BL/6J-MtgxR4531Btlr/Mmmh
Stock Number:
041771-MU
Citation ID:
RRID:MMRRC_041771-MU
Other Names:
R4531 (G1), C57BL/6J-MtgxR4531Btlr
Major Collection:

Gene Information

Trpc2
Name: transient receptor potential cation channel, subfamily C, member 2
Synonyms: TRPC2b, TRPC2a, mTrp2, trp2, Trrp2, 3010009O07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22064
Homologene: 135989
Poli
Name: polymerase (DNA directed), iota
Synonyms: Rad30b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 26447
HGNC: HGNC:9182
Homologene: 5209
Arhgef18
Name: rho/rac guanine nucleotide exchange factor (GEF) 18
Synonyms: D030053O22Rik, A430078G23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 102098
Homologene: 32254
Ttc3
Name: tetratricopeptide repeat domain 3
Synonyms: TPRD, D16Ium21, 2610202A04Rik, D16Ium21e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 22129
Homologene: 2487
Lrpprc
Name: leucine-rich PPR-motif containing
Synonyms: Lrp130, 3110001K13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 72416
Homologene: 32695
Lin54
Name: lin-54 DREAM MuvB core complex component
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231506
Homologene: 18212
Pgd
Name: phosphogluconate dehydrogenase
Synonyms: 0610042A05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 110208
HGNC: HGNC:8891
Homologene: 68268
Cspp1
Name: centrosome and spindle pole associated protein 1
Synonyms: 4930413O22Rik, 2310020J12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 211660
Homologene: 23487
E4f1
Name: E4F transcription factor 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 13560
VEGA: 17
HGNC: HGNC:3121
Homologene: 3259
Dcaf6
Name: DDB1 and CUL4 associated factor 6
Synonyms: PC326, 1200006M05Rik, Iqwd1, NRIP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 74106
Homologene: 10199
Cnot3
Name: CCR4-NOT transcription complex, subunit 3
Synonyms: A930039N10Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 232791
HGNC: HGNC:7879
Homologene: 133900
Pclo
Name: piccolo (presynaptic cytomatrix protein)
Synonyms: Acz, Pico
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 26875
Homologene: 69111
Slc24a2
Name: solute carrier family 24 (sodium/potassium/calcium exchanger), member 2
Synonyms: 6330417K15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 76376
Homologene: 10669
Cntnap4
Name: contactin associated protein-like 4
Synonyms: Caspr4, E130114F09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 170571
Homologene: 24912
Tdrd6
Name: tudor domain containing 6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 210510
Homologene: 19364
Obscn
Name: obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF
Synonyms: LOC380698, OTTMUSG00000005786
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 380698
Homologene: 70869
Vwce
Name: von Willebrand factor C and EGF domains
Synonyms: 1300015B04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 71768
VEGA: 19
Homologene: 17651
Or14c40
Name: olfactory receptor family 14 subfamily C member 40
Synonyms: GA_x6K02T2NHDJ-9457744-9456734, MOR221-3, Olfr293
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 257906
Homologene: 79385
Ubqlnl
Name: ubiquilin-like
Synonyms: LOC244179, 4922504M18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 244179
Homologene: 17034
Col22a1
Name: collagen, type XXII, alpha 1
Synonyms: C80743, 2310067L16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 69700
Homologene: 43567
Acad12
Name: acyl-Coenzyme A dehydrogenase family, member 12
Synonyms: 9330129D05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 338350
Rufy4
Name: RUN and FYVE domain containing 4
Synonyms: F930048N03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 435626
Homologene: 52359
Cyp2d10
Name: cytochrome P450, family 2, subfamily d, polypeptide 10
Synonyms: P450-2D, Cyp2d
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 13101
VEGA: 15
HGNC: HGNC:2625
Homologene: 86099
Nwd2
Name: NACHT and WD repeat domain containing 2
Synonyms: B830017A01Rik, 3110047P20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 319807
Homologene: 14974
Hmbox1
Name: homeobox containing 1
Synonyms: F830020C16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 219150
Homologene: 11589
Or8k23
Name: olfactory receptor family 8 subfamily K member 23
Synonyms: GA_x6K02T2Q125-47827833-47826892, MOR186-2, Olfr1056
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 259020
Homologene: 27328
Gpr149
Name: G protein-coupled receptor 149
Synonyms: PGR10, 9630018L10Rik, Ieda, R35
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 229357
Homologene: 16359
Kmo
Name: kynurenine 3-monooxygenase (kynurenine 3-hydroxylase)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 98256
HGNC: HGNC:6381
Homologene: 2729
Ppcs
Name: phosphopantothenoylcysteine synthetase
Synonyms: 6330579B17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 106564
Homologene: 6173
Trav6-4
Name: T cell receptor alpha variable 6-4
Synonyms: Gm13945
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 100126463
Gm26483
Name: predicted gene, 26483
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
Eif1ad19
Name: eukaryotic translation initiation factor 1A domain containing 19
Synonyms: Gm21319
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 100861908
HGNC: HGNC:3252
Homologene: 103865
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 10,066,847 bp
  • T to C, chromosome 1 at 74,147,663 bp
  • T to C, chromosome 1 at 165,411,467 bp
  • T to C, chromosome 1 at 175,659,707 bp
  • A to T, chromosome 2 at 86,355,974 bp
  • A to G, chromosome 3 at 62,602,678 bp
  • T to C, chromosome 4 at 86,991,478 bp
  • T to C, chromosome 4 at 119,422,997 bp
  • T to C, chromosome 4 at 149,156,777 bp
  • A to G, chromosome 5 at 14,775,408 bp
  • G to A, chromosome 5 at 63,725,234 bp
  • G to A, chromosome 5 at 100,446,560 bp
  • T to C, chromosome 5 at 121,598,899 bp
  • T to C, chromosome 5 at 121,598,901 bp
  • T to C, chromosome 7 at 3,658,074 bp
  • T to A, chromosome 7 at 86,664,271 bp
  • G to T, chromosome 7 at 102,095,998 bp
  • C to T, chromosome 7 at 104,149,718 bp
  • C to T, chromosome 8 at 3,447,023 bp
  • G to T, chromosome 8 at 12,663,932 bp
  • G to A, chromosome 8 at 112,810,608 bp
  • A to G, chromosome 11 at 59,007,874 bp
  • G to A, chromosome 12 at 87,773,544 bp
  • A to G, chromosome 14 at 53,454,333 bp
  • A to T, chromosome 14 at 64,825,489 bp
  • T to C, chromosome 15 at 72,007,149 bp
  • T to A, chromosome 15 at 82,405,261 bp
  • T to A, chromosome 16 at 94,466,877 bp
  • A to T, chromosome 17 at 24,445,987 bp
  • A to G, chromosome 17 at 43,628,754 bp
  • T to C, chromosome 17 at 84,712,787 bp
  • A to T, chromosome 18 at 70,517,477 bp
  • A to C, chromosome 19 at 10,664,346 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4531 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041771-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.