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Strain Name:
C57BL/6J-MtgxR4609Btlr/Mmmh
Stock Number:
041820-MU
Citation ID:
RRID:MMRRC_041820-MU
Other Names:
R4609 (G1), C57BL/6J-MtgxR4609Btlr
Major Collection:

Strain Information

Nap1l1
Name: nucleosome assembly protein 1-like 1
Synonyms: D10Ertd68e
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 53605
VEGA: 10
HGNC: HGNC:7637
Homologene: 129218
Clasp1
Name: CLIP associating protein 1
Synonyms: 5730583A19Rik, 1700030C23Rik, mCLASP1, CLASP1alpha, CLASP1, B130045P17Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 76707
Homologene: 41024
Zbtb43
Name: zinc finger and BTB domain containing 43
Synonyms: 1700010E06Rik, Zfp297b
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 71834
Homologene: 8514
Cilk1
Name: ciliogenesis associated kinase 1
Synonyms: 2210420N10Rik, Ick
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 56542
Homologene: 69218
Morc3
Name: microrchidia 3
Synonyms: 1110051N18Rik, D16Jhu32e, 1110051N18Rik, Zcwcc3
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 338467
Homologene: 32257
Trmt13
Name: tRNA methyltransferase 13
Synonyms: A930028L21Rik, 4631408H19Rik, Ccdc76
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 229780
Homologene: 6875
Zfp462
Name: zinc finger protein 462
Synonyms: Gt4-2, 9430078C22Rik, Zfpip
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 242466
Homologene: 41430
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 21,405,068 bp
  • A to C, chromosome 1 at 53,456,657 bp
  • A to T, chromosome 1 at 88,055,482 bp
  • G to A, chromosome 1 at 93,168,794 bp
  • G to A, chromosome 1 at 99,772,847 bp
  • A to G, chromosome 1 at 118,503,035 bp
  • A to T, chromosome 1 at 175,687,157 bp
  • A to T, chromosome 2 at 33,454,043 bp
  • G to T, chromosome 2 at 38,957,721 bp
  • T to C, chromosome 2 at 76,291,241 bp
  • A to G, chromosome 2 at 78,918,712 bp
  • T to C, chromosome 3 at 11,659,470 bp
  • G to T, chromosome 3 at 51,855,592 bp
  • C to T, chromosome 3 at 100,140,200 bp
  • T to C, chromosome 3 at 116,594,827 bp
  • A to T, chromosome 4 at 11,295,999 bp
  • A to T, chromosome 4 at 43,507,267 bp
  • C to T, chromosome 4 at 55,011,889 bp
  • G to A, chromosome 4 at 107,344,683 bp
  • A to G, chromosome 4 at 141,466,498 bp
  • A to G, chromosome 5 at 65,228,542 bp
  • G to T, chromosome 5 at 93,162,254 bp
  • G to A, chromosome 5 at 107,359,936 bp
  • A to G, chromosome 5 at 110,780,318 bp
  • A to T, chromosome 5 at 114,899,796 bp
  • A to T, chromosome 6 at 23,587,579 bp
  • A to T, chromosome 6 at 53,974,620 bp
  • A to C, chromosome 6 at 113,328,432 bp
  • A to T, chromosome 7 at 23,417,748 bp
  • A to G, chromosome 7 at 80,291,118 bp
  • A to G, chromosome 7 at 105,388,224 bp
  • A to T, chromosome 7 at 108,622,504 bp
  • G to T, chromosome 7 at 122,124,723 bp
  • T to C, chromosome 7 at 139,098,621 bp
  • A to T, chromosome 8 at 84,726,490 bp
  • A to G, chromosome 8 at 123,420,919 bp
  • T to C, chromosome 9 at 40,100,806 bp
  • G to A, chromosome 9 at 51,238,225 bp
  • A to G, chromosome 9 at 54,446,512 bp
  • A to G, chromosome 9 at 54,728,179 bp
  • T to C, chromosome 9 at 59,557,319 bp
  • T to C, chromosome 9 at 70,740,143 bp
  • T to A, chromosome 9 at 78,167,789 bp
  • C to T, chromosome 9 at 103,211,985 bp
  • T to C, chromosome 9 at 120,016,506 bp
  • G to T, chromosome 10 at 62,358,415 bp
  • A to T, chromosome 10 at 77,826,796 bp
  • A to G, chromosome 10 at 111,301,134 bp
  • T to A, chromosome 10 at 111,492,880 bp
  • C to T, chromosome 11 at 8,958,964 bp
  • A to T, chromosome 11 at 69,834,086 bp
  • T to C, chromosome 11 at 70,455,429 bp
  • A to G, chromosome 11 at 105,910,240 bp
  • A to G, chromosome 11 at 119,437,695 bp
  • A to T, chromosome 12 at 75,400,206 bp
  • G to T, chromosome 12 at 110,280,010 bp
  • C to T, chromosome 12 at 112,680,542 bp
  • G to T, chromosome 13 at 19,370,264 bp
  • T to C, chromosome 13 at 119,357,536 bp
  • G to A, chromosome 14 at 30,901,669 bp
  • A to T, chromosome 14 at 51,418,074 bp
  • T to C, chromosome 14 at 59,415,704 bp
  • C to T, chromosome 14 at 70,477,966 bp
  • T to A, chromosome 14 at 73,262,514 bp
  • C to A, chromosome 15 at 80,248,253 bp
  • T to C, chromosome 15 at 85,494,169 bp
  • A to G, chromosome 16 at 93,864,968 bp
  • A to T, chromosome 16 at 95,711,774 bp
  • A to T, chromosome 17 at 20,994,415 bp
  • A to T, chromosome 17 at 25,250,261 bp
  • A to T, chromosome 17 at 35,397,080 bp
  • A to T, chromosome 17 at 45,558,584 bp
  • G to A, chromosome 18 at 37,505,796 bp
  • A to T, chromosome 18 at 46,505,948 bp
  • A to G, chromosome 18 at 51,118,067 bp
  • A to T, chromosome 18 at 58,190,269 bp
  • T to A, chromosome 19 at 6,391,409 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4609 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041820-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.