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Strain Name:
C57BL/6J-MtgxR4749Btlr/Mmmh
Stock Number:
041969-MU
Citation ID:
RRID:MMRRC_041969-MU
Other Names:
R4749 (G1), C57BL/6J-MtgxR4749Btlr
Major Collection:

Strain Information

Dap3
Name: death associated protein 3
Synonyms: 4921514D13Rik, DAP-3
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 65111
HGNC: HGNC:2673
Homologene: 3404
Sort1
Name: sortilin 1
Synonyms: sortilin, neurotensin receptor 3, Ntsr3, Ntr3, 2900053A11Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 20661
Homologene: 136097
Mmp10
Name: matrix metallopeptidase 10
Synonyms: stromelysin 2
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 17384
VEGA: 9
HGNC: HGNC:7156
Homologene: 20546
Topors
Name: topoisomerase I binding, arginine/serine-rich
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 106021
Homologene: 4237
Nsfl1c
Name: NSFL1 (p97) cofactor (p47)
Synonyms: p47
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 386649
Homologene: 41114
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 58,740,272 bp
  • C to A, chromosome 1 at 87,185,583 bp
  • T to A, chromosome 1 at 136,144,749 bp
  • T to C, chromosome 1 at 163,996,785 bp
  • G to A, chromosome 1 at 171,219,562 bp
  • T to A, chromosome 1 at 173,695,614 bp
  • T to A, chromosome 1 at 185,396,130 bp
  • A to G, chromosome 2 at 11,248,157 bp
  • A to G, chromosome 2 at 82,989,285 bp
  • T to A, chromosome 2 at 88,070,612 bp
  • C to T, chromosome 2 at 89,406,255 bp
  • A to C, chromosome 2 at 104,787,304 bp
  • T to C, chromosome 2 at 112,964,405 bp
  • C to T, chromosome 2 at 125,740,737 bp
  • C to A, chromosome 2 at 130,690,867 bp
  • A to G, chromosome 2 at 151,509,606 bp
  • G to A, chromosome 2 at 157,001,740 bp
  • T to C, chromosome 3 at 36,566,242 bp
  • A to G, chromosome 3 at 59,097,466 bp
  • C to T, chromosome 3 at 87,089,151 bp
  • A to G, chromosome 3 at 88,926,310 bp
  • A to T, chromosome 3 at 89,150,063 bp
  • G to A, chromosome 3 at 98,742,615 bp
  • T to G, chromosome 3 at 108,356,323 bp
  • A to T, chromosome 4 at 40,261,015 bp
  • T to C, chromosome 4 at 63,995,639 bp
  • T to C, chromosome 4 at 123,826,901 bp
  • C to A, chromosome 4 at 152,330,057 bp
  • A to G, chromosome 5 at 3,968,737 bp
  • G to A, chromosome 5 at 65,009,066 bp
  • T to C, chromosome 5 at 76,858,834 bp
  • C to T, chromosome 5 at 92,304,977 bp
  • T to C, chromosome 5 at 114,255,567 bp
  • T to A, chromosome 5 at 115,614,402 bp
  • A to G, chromosome 6 at 7,866,379 bp
  • T to C, chromosome 6 at 39,090,344 bp
  • A to G, chromosome 6 at 42,290,197 bp
  • T to C, chromosome 6 at 84,067,008 bp
  • T to C, chromosome 6 at 88,891,991 bp
  • T to A, chromosome 6 at 146,650,101 bp
  • T to C, chromosome 7 at 16,498,626 bp
  • T to C, chromosome 7 at 27,658,361 bp
  • C to T, chromosome 7 at 35,200,762 bp
  • T to G, chromosome 7 at 106,081,907 bp
  • T to G, chromosome 7 at 141,861,448 bp
  • A to G, chromosome 8 at 4,813,143 bp
  • T to A, chromosome 8 at 5,639,918 bp
  • A to G, chromosome 8 at 27,114,197 bp
  • C to T, chromosome 8 at 42,247,353 bp
  • A to T, chromosome 8 at 122,383,643 bp
  • A to T, chromosome 8 at 122,486,939 bp
  • G to T, chromosome 8 at 122,498,206 bp
  • T to C, chromosome 8 at 125,887,588 bp
  • A to T, chromosome 9 at 7,508,168 bp
  • C to T, chromosome 9 at 58,234,652 bp
  • A to G, chromosome 9 at 121,741,693 bp
  • C to A, chromosome 10 at 20,132,052 bp
  • T to A, chromosome 10 at 20,132,053 bp
  • A to T, chromosome 10 at 59,424,082 bp
  • A to G, chromosome 10 at 60,716,326 bp
  • A to G, chromosome 10 at 86,701,808 bp
  • T to C, chromosome 10 at 120,084,849 bp
  • A to G, chromosome 11 at 29,482,720 bp
  • A to G, chromosome 11 at 55,311,468 bp
  • C to A, chromosome 11 at 55,536,384 bp
  • G to A, chromosome 11 at 65,834,115 bp
  • A to T, chromosome 11 at 69,911,068 bp
  • G to A, chromosome 11 at 73,354,496 bp
  • A to G, chromosome 11 at 77,469,148 bp
  • T to A, chromosome 11 at 102,690,242 bp
  • A to G, chromosome 11 at 105,935,882 bp
  • T to C, chromosome 11 at 116,541,908 bp
  • T to C, chromosome 12 at 70,248,273 bp
  • T to A, chromosome 12 at 71,151,397 bp
  • T to A, chromosome 12 at 73,692,960 bp
  • A to T, chromosome 13 at 48,821,709 bp
  • G to A, chromosome 13 at 59,718,358 bp
  • A to T, chromosome 14 at 20,524,062 bp
  • C to T, chromosome 14 at 31,529,515 bp
  • T to A, chromosome 14 at 35,580,687 bp
  • T to A, chromosome 14 at 44,506,012 bp
  • A to G, chromosome 14 at 50,238,733 bp
  • C to T, chromosome 14 at 64,029,800 bp
  • T to A, chromosome 14 at 79,453,287 bp
  • A to T, chromosome 15 at 8,365,829 bp
  • T to A, chromosome 15 at 55,452,336 bp
  • T to A, chromosome 15 at 76,347,567 bp
  • T to C, chromosome 16 at 95,361,170 bp
  • T to C, chromosome 17 at 6,740,945 bp
  • G to A, chromosome 17 at 13,070,945 bp
  • A to G, chromosome 17 at 20,419,751 bp
  • C to A, chromosome 17 at 25,844,274 bp
  • T to C, chromosome 17 at 26,116,783 bp
  • G to C, chromosome 17 at 56,771,274 bp
  • C to T, chromosome 17 at 86,944,707 bp
  • A to G, chromosome 18 at 20,446,831 bp
  • A to G, chromosome 18 at 22,516,769 bp
  • C to T, chromosome 18 at 35,652,816 bp
  • A to T, chromosome 18 at 60,917,461 bp
  • A to T, chromosome 18 at 78,155,581 bp
  • C to T, chromosome 19 at 12,935,217 bp
  • T to C, chromosome 19 at 45,451,171 bp
  • A to G, chromosome 19 at 56,380,237 bp
  • A to T, chromosome 19 at 57,215,721 bp
  • A to G, chromosome X at 147,193,797 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4749 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

The donor or their institution limits the distribution to non-profit institutions only.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
041969-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.