Strain Name:
B6;129P2-Ptentm1Mak/Mmjax
Stock Number:
042059-JAX
Citation ID:
RRID:MMRRC_042059-JAX
Other Names:
PTEN, MMAC, TEP1

Gene Information

Ptentm1Mak
Name: phosphatase and tensin homolog; targeted mutation 1, Tak W Mak
Synonyms: mPTEN3-5, Pten-, mPTEN-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Knockout
Pten
Name: phosphatase and tensin homolog
Synonyms: TEP1, A130070J02Rik, MMAC1, B430203M17Rik, 2310035O07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Knockout
NCBI: 19211
HGNC: HGNC:9588
Homologene: 265
Genetic Alterations
Exons 3 to 5 deletion with Neo insertion in reverse orientation
Genotype Determination
ES Cell Line
E14 derived from 129P2/OlaHsd
Phenotype

Homozygous: Homozygous mutant mice lacking exons 3-5 of the PTEN gene (mPTEN3-5) had severely expanded and abnormally patterned cephalic and caudal regions at day 8.5 of gestation. Embryonic death occurred by day 9.5 and was associated with defective chorio-allantoic development.

Heterozygous: Heterozygous mPTEN3-5 mice had an increased incidence of tumors, especially T-cell lymphomas; gamma-irradiation reduced the time lapse of tumor formation. DNA analysis of these tumors revealed the deletion of the mPTEN gene due to loss of heterozygosity of the wild-type allele. Tumors associated with loss of heterozygosity in mPTEN showed elevated phosphorylation of protein kinase B (PKB, also known as Akt kinase), thus providing a functional connection between mPTEN and a murine proto-oncogene (c-Akt) involved in the development of lymphomas.

Mammalian Phenotype Terms
Allelic Composition: (Genetic Background: )

MeSH Terms
  • Animals
  • Embryonic and Fetal Development/genetics
  • Exons
  • Female
  • Fetal Death/genetics
  • Gamma Rays
  • Genes, Tumor Suppressor
  • Genetic Predisposition to Disease/genetics
  • Genotype
  • Lymphoma, T-Cell/genetics
  • Mice
  • Mice, Mutant Strains
  • Neoplasms/genetics
  • PTEN Phosphohydrolase
  • Phenotype
  • Phosphoric Monoester Hydrolases/genetics
  • Polymerase Chain Reaction
  • Pregnancy
  • Proto-Oncogenes
  • Recombination, Genetic
  • Sequence Deletion
  • Tumor Suppressor Proteins
Strain Development
129 Ola ES cells were targeted by homologous recombination and then injected into C57BL/6 blastocysts. The resulting chimeras were backcrossed into C57BL/6J mice from Jackson (current stock number 000664)
Suggested Control Mice
Wild-type littermates
  • Cancer
  • Developmental Biology
Donor
Tak Mak, Ph.D., Campbell Family Institute for Breast Cancer Research.
Primary Reference

Suzuki A, de la Pompa JL, Stambolic V, Elia AJ, Sasaki T, del Barco Barrantes I, Ho A, Wakeham A, Itie A, Khoo W, Fukumoto M, Mak TW. High cancer susceptibility and embryonic lethality associated with mutation of the PTEN tumor suppressor gene in mice. Curr Biol. 1998 Oct 22;8(21):1169-78. (Medline PMID: 9799734)

Colony and Husbandry Information

For more information about this colony's health status contact csmmrrc@jax.org
Coat Color
Black
Other
N/A
MMRRC Breeding System
Backcross
Generation
N9+
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: N/A N/A
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 8 to 12 months 8 to 12 months
Average litter size
4-6
Recommended wean age
3-4 weeks

Order Request Information

Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at csmmrrc@jax.org for more details.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042059-JAX-HET-F
042059-JAX-HET-M
042059-JAX-WT-F
042059-JAX-WT-M
Heterozygous / Hemizygous Female
Heterozygous / Hemizygous Male
Wild Type Female
Wild Type Male
$218.00 / Non-Profit Per Mouse The csmmrrc@jax.org may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.