Strain Name:
B6J.Cg-Sptlc1tm1.1Rlp/Mmucd
Stock Number:
042294-UCD
Citation ID:
RRID:MMRRC_042294-UCD
Other Names:
Sptlc1fl/fl,

Gene Information

Sptlc1tm1.1Rlp
Name: serine palmitoyltransferase, long chain base subunit 1; targeted mutation 1.1, Richard L Proia
Synonyms: Stplc1fl
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Conditional
Sptlc1
Name: serine palmitoyltransferase, long chain base subunit 1
Synonyms: Lcb1, Spt1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
Alteration at locus: Conditional
NCBI: 268656
VEGA: 13
Homologene: 4681
Genetic Alterations
Inserted flox sites flanking exons 4 and 5
ES Cell Line
iTL1 derived from 129S6/SvEvTac
Phenotype

Homozygous: Normal

Hetero/Hemizygous: Normal

Cre-excised Phenotype: Undetermined 

MeSH Terms
  • Adipocytes/cytology
  • Adiposity
  • Animals
  • Cell Differentiation
  • Cell Survival
  • Gene Deletion
  • Homeostasis
  • Inflammation
  • Insulin Resistance
  • Lipid Metabolism
  • Lipogenesis
  • Macrophages/metabolism
  • Mice
  • Mice, Knockout
  • Serine C-Palmitoyltransferase/genetics
  • Serine C-Palmitoyltransferase/metabolism
  • Sphingolipids/biosynthesis
Strain Development
To generate Sptlc1fl mice, an Sptlc1 gene targeting vector was constructed to flank exons 4 and 5, which encode part of the active site, with LoxP sites 973 bp apart. A short homology (∼2 kb) arm extended 5′ to exon 4 and a long homology arm (∼5.7 kb) was located 3′ to exon 5. The neomycin (neo) gene was present between the short homology arm and exon 4 and was flanked by FRT sequences. The targeting vector was linearized and transfected by electroporation into BA1 embryonic stem cells. Following selection with G418, surviving clones were expanded and tested by PCR for identification of the positive clones, which were then confirmed by Southern blotting . Confirmed clones were microinjected into C57BL/6 mouse blastocysts to obtain germ line transmission. The generated chimeras and subsequently their offspring were genotyped to determine the presence of the Sptlc1fl(neo) gene. Subsequently, to avoid interference with endogenous gene transcription, the neomycin gene was eliminated in the germ line by crossing the Sptlc1fl(neo) mice with mice expressing FLP recombinase (The Jackson Laboratory, Bar Harbor, ME). The following primers were used for PCR to distinguish the WT Sptlc1 from the Sptlc1fl allele: 5′-GGG TTC TAT GGC ACA TTT GGT AAG-3′ (forward primer), and 5′-CTG TTA CTT CTT GCC AGT GGA C-3′ (reverse primer), which generate products of 350 bp (WT) and 425 bp (Sptlc1fl).
Suggested Control Mice
Wild-type littermates
  • Apoptosis
  • Cancer
  • Cardiovascular
  • Cell Biology
  • Dermatology
  • Developmental Biology
  • Diabetes
  • Hematology
  • Immunology and Inflammation
  • Internal Organ
  • Metabolism
  • Models for Human Disease
  • Neurobiology
  • Obesity
  • Reproduction
  • Research Tools
  • Sensorineural
Donor
Dr. Richard Proia, NIDDK/NIH
Primary Reference

Alexaki A, Clarke BA, Gavrilova O, Ma Y, Zhu H, Ma X, Xu L, Tuymetova G, Larman BC, Allende ML, Dunn TM, Proia RL. De Novo Sphingolipid Biosynthesis Is Required for Adipocyte Survival and Metabolic Homeostasis. J Biol Chem. 2017 Mar 3;292(9):3929-3939. doi: 10.1074/jbc.M116.756460. Epub 2017 Jan 18. (Medline PMID: 28100772)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .
Coat Color
Black
MMRRC Breeding System
Sib-mating
Generation
7
Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 4 to 6 months 8 to 12 months
Bred to Homozygosity
No
Average litter size
4-6
Recommended wean age
3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042294-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
042294-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.