Strain Name:
129S;B6-Tg(CAG-H3f3a/EGFP)1Dean/Mmnc
Stock Number:
042299-UNC
Citation ID:
RRID:MMRRC_042299-UNC
Other Names:
H3.3-GFP, H3.3-EGFP

Gene Information

Allele: Tg(CAG-H3f3a/EGFP)1Dean (Drosophila melanogaster (fruit fly))
Name: transgene insertion 1, Jurrien Dean
Chromosome: random insertion
Alteration at locus: Transgenic
Monarch Initiative (Disease and Phenotype Associations):
Promoter: CAG (Multi-species)
Name: CMV early enhancer/chicken beta actin promoter/rabbit beta globin splice acceptor
Alteration at locus: Transgenic
Monarch Initiative (Disease and Phenotype Associations):
Transgene: EGFP (Aequorea victoria (jellyfish))
Name: enhanced green fluorescent protein
Alteration at locus: Transgenic
Monarch Initiative (Disease and Phenotype Associations):
Genetic Alterations
Transgenic mice expressing a fusion gene of Drosophila melanogaster histone H3.3 (NCBI Gene ID:33736) and EGFP, driven by a CAG promoter.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    N/A
    Phenotype

    Homozygous: normal

    Hetero/Hemizygous: normal

    MeSH Terms
      Strain Development
      To establish pCAG-H3.3eGFP transgenic mice, the H3.3eGFP fragment from H3.3-pEGFP-N1 (gift from Dr Philippe Collas; PMID:20375147) was subcloned into pCAGEN. To test for fertility, female mice were co-caged with WT fertile males.
      Suggested Control Mice
      Wild-type littermates
      • Cell Biology
      • Developmental Biology
      • Models for Human Disease
      • Reproduction
      • Research Tools
      Donor
      Jurrien Dean, M.D., National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health.

      Colony and Husbandry Informationon

      Colony Surveillance Program and Current Health Reports

      Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu .
      Coat Color
      dark grey
      MMRRC Breeding System
      Sib-mating
      Generation
      N10F7
      Overall Breeding Performance
      Good
      NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
      Viability and Fertility: Female Male Comments
      Homozygotes are viable: Yes Yes
      Homozygotes are fertile: Yes Yes
      Hetero/Hemizygotes are fertile: Yes Yes
      Age Reproductive Decline: 4 to 6 months 6 to 8 months
      Bred to Homozygosity
      No
      Average litter size
      4-6
      Recommended wean age
      3 weeks

      Order Request Information

      Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

      Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

      The donor or their institution limits the distribution to non-profit institutions only.

      Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

      Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
      MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
      042299-UNC-EMBRYO Cryo-preserved embryos $1,363.00 / Non-Profit Aliquot Approximate quantity3 : 20-40 embryos / aliquot
      042299-UNC-SPERM Cryo-preserved spermatozoa $548.00 / Non-Profit Aliquot Approximate quantity2
      042299-UNC-RESUS Litter recovered from cryo-archive $2,829.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.

      Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

      1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

      2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

      3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

      To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.