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Strain Name:
C57BL/6J-MtgxR4850Btlr/Mmmh
Stock Number:
042462-MU
Citation ID:
RRID:MMRRC_042462-MU
Other Names:
R4850 (G1), C57BL/6J-MtgxR4850Btlr
Major Collection:

Strain Information

Slc32a1
Name: solute carrier family 32 (GABA vesicular transporter), member 1
Synonyms: R75019, VGAT, Viaat
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22348
Homologene: 56451
Prkcd
Name: protein kinase C, delta
Synonyms: PKCdelta, PKC[d], Pkcd, D14Ertd420e
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 18753
HGNC: HGNC:9399
Homologene: 55963
Trp53bp1
Name: transformation related protein 53 binding protein 1
Synonyms: 53BP1, p53BP1
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 27223
Homologene: 4137
Ankrd17
Name: ankyrin repeat domain 17
Synonyms: 4933425K22Rik, Gtar, A130069E23Rik
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 81702
Homologene: 82403
Zfp638
Name: zinc finger protein 638
Synonyms: Np220, Zfml
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 18139
Homologene: 7447
Adgrl2
Name: adhesion G protein-coupled receptor L2
Synonyms: Lphh1, Lec1, Lphn2
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 99633
Homologene: 22712
Nup205
Name: nucleoporin 205
Synonyms: 3830404O05Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 70699
Homologene: 45971
Slc30a7
Name: solute carrier family 30 (zinc transporter), member 7
Synonyms: ZnT7, 1810059J10Rik, ZnT-7, 4833428C12Rik, 2610034N15Rik
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 66500
Homologene: 11332
Urb1
Name: URB1 ribosome biogenesis 1 homolog (S. cerevisiae)
Synonyms: 5730405K23Rik, 4921511H13Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 207932
Homologene: 45941
Xdh
Name: xanthine dehydrogenase
Synonyms: Xox-1, Xox1, Xor, xanthine oxidase, XO
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 22436
VEGA: 17
Homologene: 324
Tenm2
Name: teneurin transmembrane protein 2
Synonyms: Ten-m2, D3Bwg1534e, 9330187F13Rik, 2610040L17Rik, Odz2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 23964
Homologene: 22672
Hmg20b
Name: high mobility group 20B
Synonyms: Smarce1r, Hmgx2, BRCA2-associated factor 35, BRAF35, Hmgxb2
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 15353
HGNC: HGNC:5002
Homologene: 74949
Pex1
Name: peroxisomal biogenesis factor 1
Synonyms: ZWS1, 5430414H02Rik, E330005K07Rik, peroxisome biogenesis factor 1
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 71382
HGNC: HGNC:8850
Homologene: 27006
Ryr2
Name: ryanodine receptor 2, cardiac
Synonyms: 9330127I20Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 20191
VEGA: 13
Homologene: 37423
Sfxn5
Name: sideroflexin 5
Synonyms: C230001H08Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 94282
Homologene: 16936
Zwint
Name: ZW10 interactor
Synonyms: 2600001N01Rik, 2010007E07Rik, D10Ertd749e
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 52696
Homologene: 48496
Cic
Name: capicua transcriptional repressor
Synonyms: 1200010B10Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 71722
Homologene: 87637
Fabp3
Name: fatty acid binding protein 3, muscle and heart
Synonyms: H-FABP, Fabph-4, Fabph-1, Fabph4, Fabph1, Mdgi, Fabp3
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 14077
HGNC: HGNC:3557
Homologene: 68379
Slit2
Name: slit guidance ligand 2
Synonyms: Slil3, Drad-1, E130320P19Rik, E030015M03Rik, b2b1200.1Clo
Type: Gene
Species: Mouse
Chromosome: 5
NCBI: 20563
Homologene: 3516
Dsp
Name: desmoplakin
Synonyms: DP, 2300002E22Rik, 5730453H04Rik, rul
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 109620
HGNC: HGNC:3052
Homologene: 37922
Enpp5
Name: ectonucleotide pyrophosphatase/phosphodiesterase 5
Synonyms: D17Abb1e
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 83965
Homologene: 23313
Dync2h1
Name: dynein cytoplasmic 2 heavy chain 1
Synonyms: DHC1b, DHC2, 4432416O06Rik, D330044F14Rik, D030010H02Rik, Dnchc2, b2b414Clo, m407Asp, m152Asp
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 110350
HGNC: HGNC:2962
Homologene: 14468
Pros1
Name: protein S (alpha)
Synonyms: protein S
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 19128
HGNC: HGNC:9456
Homologene: 264
Cand2
Name: cullin associated and neddylation dissociated 2 (putative)
Synonyms: Tp120b, 2210404G23Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 67088
Homologene: 41657
Arap1
Name: ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1
Synonyms: 2410002L19Rik, Centd2
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 69710
Homologene: 12326
Mtus1
Name: mitochondrial tumor suppressor 1
Synonyms: MD44, MTSG1, Atip1, B430305I03Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 102103
Homologene: 100292
Eml5
Name: echinoderm microtubule associated protein like 5
Synonyms: C130068M19Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 319670
VEGA: 12
Homologene: 26807
Atad2b
Name: ATPase family, AAA domain containing 2B
Synonyms: 1110014E10Rik, D530031C13Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 320817
VEGA: 12
Homologene: 86351
Cep295
Name: centrosomal protein 295
Synonyms: LOC382128, 5830418K08Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 319675
Homologene: 27936
Nacc2
Name: nucleus accumbens associated 2, BEN and BTB (POZ) domain containing
Synonyms: 0610020I02Rik, C030048H19Rik, Btbd14a
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 67991
Homologene: 75239
Ttn
Name: titin
Synonyms: connectin, L56, mdm, 1100001C23Rik, D830007G01Rik, 2310036G12Rik, 2310074I15Rik, 2310057K23Rik, D330041I19Rik, shru
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 22138
Homologene: 130650
Trappc11
Name: trafficking protein particle complex 11
Synonyms: D030016E14Rik
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 320714
Homologene: 11076
Cplane1
Name: ciliogenesis and planar polarity effector 1
Synonyms: b2b012Clo, Jbts17, Hug, 2410089E03Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 73692
Homologene: 11315
Rab44
Name: RAB44, member RAS oncogene family
Synonyms: 9830134C10Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 442827
Homologene: 53300
Nfatc1
Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 1
Synonyms: NFAT2, NFATc, NF-ATc, 2210017P03Rik
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 18018
VEGA: 18
HGNC: HGNC:7775
Homologene: 32336
Mtpap
Name: mitochondrial poly(A) polymerase
Synonyms: 0610027A18Rik, Papd1, Tent6
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 67440
VEGA: 18
Homologene: 10008
Rp1
Name: retinitis pigmentosa 1 (human)
Synonyms: oxygen-regulated protein 1, Orp1, mG145, Dcdc3, Rp1h
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 19888
Homologene: 4564
Or13f5
Name: olfactory receptor family 13 subfamily F member 5
Synonyms: GA_x6K02T2N78B-7168533-7167574, MOR262-2, Olfr275
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 258857
Homologene: 17418
Kcnt1
Name: potassium channel, subfamily T, member 1
Synonyms: slo2, Slack, C030030G16Rik
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227632
Homologene: 11055
Vwde
Name: von Willebrand factor D and EGF domains
Synonyms: LOC232585
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 232585
Homologene: 35456
Slco6b1
Name: solute carrier organic anion transporter family, member 6b1
Synonyms: 1700022M03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 67854
Homologene: 12199
Stat6
Name: signal transducer and activator of transcription 6
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 20852
Homologene: 2373
Sncaip
Name: synuclein, alpha interacting protein (synphilin)
Synonyms: 4933427B05Rik, SYPH1, synphilin-1
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 67847
Homologene: 3987
Akr1d1
Name: aldo-keto reductase family 1, member D1
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 208665
HGNC: HGNC:388
Homologene: 55943
Or8u9
Name: olfactory receptor family 8 subfamily U member 9
Synonyms: GA_x6K02T2Q125-47640742-47639798, MOR185-4, Olfr1044
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 259013
Homologene: 100542
Acsf3
Name: acyl-CoA synthetase family member 3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 257633
Homologene: 14958
Fn3krp
Name: fructosamine 3 kinase related protein
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 238024
Homologene: 11093
Abcb10
Name: ATP-binding cassette, sub-family B member 10
Synonyms: ABC-me
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 56199
HGNC: HGNC:41
Homologene: 6474
Prdm13
Name: PR domain containing 13
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 230025
Homologene: 11000
Vmn2r95
Name: vomeronasal 2, receptor 95
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 328759
Homologene: 115024
Rangrf
Name: RAN guanine nucleotide release factor
Synonyms: Mog1, Rangnrf, 2400006H24Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 57785
Homologene: 41138
Kmo
Name: kynurenine 3-monooxygenase
Synonyms: kynurenine 3-hydroxylase
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 98256
HGNC: HGNC:6381
Homologene: 2729
Garin2
Name: golgi associated RAB2 interactor 2
Synonyms: 4930516C23Rik, 4921509E07Rik, Fam71d
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 70897
Homologene: 49887
Smpd1
Name: sphingomyelin phosphodiesterase 1, acid lysosomal
Synonyms: A-SMase, aSMase, ASM, acid sphingomyelinase, Zn-SMase
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 20597
Homologene: 457
Terb1
Name: telomere repeat binding bouquet formation protein 1
Synonyms: 4930532D21Rik, Ccdc79
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 320022
Homologene: 18330
Cryge
Name: crystallin, gamma E
Synonyms: Cryg-6
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12968
HGNC: HGNC:2411
Homologene: 128417
Slc26a8
Name: solute carrier family 26, member 8
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 224661
Homologene: 27095
Hsd3b6
Name: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 6
Synonyms: 3beta-HSD VI
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 15497
Homologene: 133013
Cnga3
Name: cyclic nucleotide gated channel alpha 3
Synonyms: CNG3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12790
HGNC: HGNC:2150
Homologene: 994
Sbspon
Name: somatomedin B and thrombospondin, type 1 domain containing
Synonyms: LOC226866, Gm106
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 226866
Homologene: 45950
Atp6v0d1
Name: ATPase, H+ transporting, lysosomal V0 subunit D1
Synonyms: Ac39, Atp6d, P39, Vma6, lysosomal 38kDa
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 11972
Homologene: 3444
Maf1
Name: MAF1 homolog, negative regulator of RNA polymerase III
Synonyms: 1110068E11Rik, Maf1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 68877
Homologene: 49867
Haghl
Name: hydroxyacylglutathione hydrolase-like
Synonyms: C330022E15Rik, 1500017E18Rik, 2810014I23Rik
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 68977
Homologene: 57062
Gm26672
Name: predicted gene, 26672
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
Gm10718
Name: predicted gene 10718
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Nphs1os
Name: nephrosis 1 homolog, nephrin, opposite strand
Synonyms: Nphs1as
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 445267
H2bc11
Name: H2B clustered histone 11
Synonyms: Hist1h2bj
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 319183
HGNC: HGNC:4748
Homologene: 136771
RP23-186B18.8
Name:
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
Igkv5-48
Name: immunoglobulin kappa variable 5-48
Synonyms: ENSMUSG00000029975, Gm10881
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 619846
Rpl7a-ps8
Name: ribosomal protein L7A, pseudogene 8
Synonyms: Gm4970
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 243872
Apela
Name: apelin receptor early endogenous ligand
Synonyms: Ende, Gm10664, Ela, Elabela
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 100038489
Pcdhga8
Name: protocadherin gamma subfamily A, 8
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 93716
HGNC: HGNC:8706
Homologene: 57162
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to A, chromosome 1 at 4,348,675 bp
  • T to A, chromosome 1 at 15,858,968 bp
  • G to T, chromosome 1 at 37,258,006 bp
  • G to T, chromosome 1 at 65,051,052 bp
  • T to C, chromosome 1 at 96,911,833 bp
  • A to T, chromosome 1 at 166,642,339 bp
  • A to G, chromosome 1 at 175,637,577 bp
  • T to C, chromosome 2 at 25,908,100 bp
  • C to T, chromosome 2 at 26,090,231 bp
  • T to A, chromosome 2 at 35,283,900 bp
  • T to G, chromosome 2 at 76,781,555 bp
  • T to A, chromosome 2 at 86,171,671 bp
  • T to A, chromosome 2 at 121,205,113 bp
  • T to C, chromosome 2 at 158,614,192 bp
  • A to G, chromosome 3 at 19,243,117 bp
  • G to A, chromosome 3 at 98,807,905 bp
  • A to G, chromosome 3 at 115,993,008 bp
  • G to A, chromosome 3 at 148,859,020 bp
  • C to A, chromosome 4 at 21,678,243 bp
  • T to C, chromosome 4 at 52,825,450 bp
  • C to T, chromosome 4 at 130,312,387 bp
  • C to T, chromosome 5 at 3,624,426 bp
  • T to C, chromosome 5 at 48,224,774 bp
  • A to T, chromosome 5 at 90,264,786 bp
  • A to T, chromosome 6 at 13,196,048 bp
  • A to G, chromosome 6 at 35,230,530 bp
  • A to G, chromosome 6 at 37,554,587 bp
  • A to G, chromosome 6 at 69,726,796 bp
  • T to C, chromosome 6 at 83,979,475 bp
  • C to A, chromosome 6 at 84,097,715 bp
  • T to C, chromosome 6 at 85,332,376 bp
  • A to T, chromosome 6 at 115,801,948 bp
  • T to C, chromosome 7 at 19,953,934 bp
  • G to A, chromosome 7 at 25,272,902 bp
  • T to G, chromosome 7 at 30,463,232 bp
  • T to C, chromosome 7 at 101,398,791 bp
  • A to G, chromosome 7 at 105,555,985 bp
  • A to C, chromosome 8 at 41,084,470 bp
  • G to A, chromosome 8 at 47,496,983 bp
  • A to T, chromosome 8 at 65,013,418 bp
  • T to A, chromosome 8 at 104,485,425 bp
  • A to G, chromosome 8 at 105,530,840 bp
  • T to C, chromosome 8 at 122,817,436 bp
  • C to T, chromosome 8 at 123,982,690 bp
  • A to T, chromosome 9 at 3,023,716 bp
  • T to C, chromosome 9 at 7,134,364 bp
  • T to C, chromosome 9 at 15,322,704 bp
  • T to A, chromosome 10 at 72,655,956 bp
  • T to A, chromosome 10 at 81,346,927 bp
  • T to C, chromosome 10 at 127,657,150 bp
  • A to C, chromosome 11 at 36,023,488 bp
  • A to G, chromosome 11 at 68,973,640 bp
  • A to T, chromosome 11 at 121,425,053 bp
  • G to A, chromosome 12 at 4,943,251 bp
  • A to G, chromosome 12 at 78,715,153 bp
  • T to C, chromosome 12 at 98,790,619 bp
  • A to T, chromosome 13 at 11,668,820 bp
  • G to A, chromosome 13 at 11,745,752 bp
  • G to T, chromosome 13 at 22,043,251 bp
  • T to A, chromosome 13 at 38,192,469 bp
  • A to G, chromosome 14 at 30,599,743 bp
  • T to C, chromosome 15 at 8,262,938 bp
  • T to C, chromosome 15 at 76,352,962 bp
  • T to A, chromosome 16 at 26,363,163 bp
  • A to T, chromosome 16 at 62,885,524 bp
  • A to T, chromosome 16 at 90,795,414 bp
  • T to C, chromosome 17 at 18,451,653 bp
  • T to C, chromosome 17 at 25,783,006 bp
  • T to A, chromosome 17 at 28,654,883 bp
  • A to G, chromosome 17 at 29,140,089 bp
  • T to C, chromosome 17 at 44,080,954 bp
  • A to G, chromosome 17 at 73,898,335 bp
  • C to T, chromosome 18 at 4,387,044 bp
  • A to T, chromosome 18 at 37,727,709 bp
  • C to A, chromosome 18 at 37,755,973 bp
  • A to T, chromosome 18 at 52,871,384 bp
  • T to A, chromosome 18 at 80,697,865 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4850 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
042462-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.