Strain Name:
C57BL/6J-MtgxR4949Btlr/Mmmh
Stock Number:
042546-MU
Citation ID:
RRID:MMRRC_042546-MU
Other Names:
R4949 (G1), C57BL/6J-MtgxR4949Btlr
Major Collection:

Gene Information

Scn8a
Name: sodium channel, voltage-gated, type VIII, alpha
Synonyms: Nav1.6, mnd2, mnd-2, motor end-plate disease, nur14, ataxia 3, seal, nmf58, NaCh6, nmf335, NMF335, C630029C19Rik, nmf2, med
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 20273
Homologene: 7927
Bcl2l13
Name: BCL2-like 13 (apoptosis facilitator)
Synonyms: E430016C20Rik, BCL-RAMBO, Mil1, Mil-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 94044
Homologene: 9111
Rheb
Name: Ras homolog enriched in brain
Synonyms: Rheb1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 19744
Homologene: 123916
Rps6kc1
Name: ribosomal protein S6 kinase polypeptide 1
Synonyms: B130003F20Rik, RPK118
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 320119
Homologene: 8244
Dync1h1
Name: dynein cytoplasmic 1 heavy chain 1
Synonyms: dynein heavy chain, retrograde transport, MAP1C, Dnec1, Dnchc1, Loa, Swl, 9930018I23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 13424
HGNC: HGNC:2961
Homologene: 1053
Lpin2
Name: lipin 2
Synonyms: 2610511G02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 64898
Homologene: 8769
N4bp2
Name: NEDD4 binding protein 2
Synonyms: B3bp, LOC386488, LOC333789
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 333789
Homologene: 32396
Rph3a
Name: rabphilin 3A
Synonyms: 2900002P20Rik, Doc2 family
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 19894
Homologene: 7921
Cdhr5
Name: cadherin-related family member 5
Synonyms: 1810074H01Rik, Mucdhl, Mupcdh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 72040
HGNC: HGNC:7521
Homologene: 69345
Tes
Name: testis derived transcript
Synonyms: testin2, Tes2, D6Ertd352e, testin, Tes1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 21753
Homologene: 41051
Dsg2
Name: desmoglein 2
Synonyms: D18Ertd293e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 13511
HGNC: HGNC:3049
Homologene: 1464
Klf6
Name: Kruppel-like factor 6
Synonyms: Zf9, Copeb, Ierepo1, CPBP, BCD1, Ierepo3, FM2, FM6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 23849
VEGA: 13
HGNC: HGNC:2235
Homologene: 996
Lsm1
Name: LSM1 homolog, mRNA degradation associated
Synonyms: 2810025O06Rik, U6 small nuclear RNA associated
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 67207
Homologene: 40945
Clp1
Name: CLP1, cleavage and polyadenylation factor I subunit
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 98985
Homologene: 4975
Tmprss4
Name: transmembrane protease, serine 4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 214523
Homologene: 80930
Kifc5b
Name: kinesin family member C5B
Synonyms: kinesin family c-terminal 5B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 16580
VEGA: 17
HGNC: HGNC:6389
Homologene: 83229
Tas2r124
Name: taste receptor, type 2, member 124
Synonyms: mt2r50, mGR24, Tas2r24, T2R24
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 387351
Homologene: 87293
Ttpal
Name: tocopherol (alpha) transfer protein-like
Synonyms: 3110080A02Rik, 5830472M02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 76080
Homologene: 41562
Insr
Name: insulin receptor
Synonyms: CD220, 4932439J01Rik, IR, IR-B, IR-A, D630014A15Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 16337
HGNC: HGNC:6091
Homologene: 20090
Tmbim1
Name: transmembrane BAX inhibitor motif containing 1
Synonyms: 2310061B02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 69660
Homologene: 36404
Ift140
Name: intraflagellar transport 140
Synonyms: Wdtc2, Tce5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 106633
Homologene: 40979
Map7d1
Name: MAP7 domain containing 1
Synonyms: Rprc1, Mtap7d1, Parcc1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 245877
Homologene: 17009
Kcnb1
Name: potassium voltage gated channel, Shab-related subfamily, member 1
Synonyms: Shab, Kcr1-1, Kv2.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 16500
HGNC: HGNC:6231
Homologene: 37988
Jkamp
Name: JNK1/MAPK8-associated membrane protein
Synonyms: 1200003C05Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 104771
Homologene: 9524
Rpain
Name: RPA interacting protein
Synonyms: 2400006N03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 69723
Homologene: 13006
Serpinb9e
Name: serine (or cysteine) peptidase inhibitor, clade B, member 9e
Synonyms: ovalbumin, NK26, Spi14
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 20710
HGNC: HGNC:8955
Homologene: 69093
Cylc2
Name: cylicin, basic protein of sperm head cytoskeleton 2
Synonyms: 4930488P18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 74914
HGNC: HGNC:2583
Homologene: 136270
Vmn1r230
Name: vomeronasal 1 receptor 230
Synonyms: V1re8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 171231
Homologene: 136306
Itpripl1
Name: inositol 1,4,5-triphosphate receptor interacting protein-like 1
Synonyms: 1700041B20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 73338
Homologene: 77641
Catsperg1
Name: cation channel sperm associated auxiliary subunit gamma 1
Synonyms: A230107C01Rik, Catsperg
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 320225
Homologene: 10915
Ido2
Name: indoleamine 2,3-dioxygenase 2
Synonyms: Ido2, C230043N17Rik, Indol1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 209176
Homologene: 48830
Dnd1
Name: DND microRNA-mediated repression inhibitor 1
Synonyms: Ter, RBMS4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 213236
Homologene: 65262
Sdsl
Name: serine dehydratase-like
Synonyms: 4432411H13Rik, SDS-RS1, SDH1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 257635
Homologene: 44224
Nt5el
Name: 5' nucleotidase, ecto-like
Synonyms: 4933425L06Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 66763
Homologene: 12028
Ttll1
Name: tubulin tyrosine ligase-like 1
Synonyms: 6330444E16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 319953
HGNC: HGNC:1312
Homologene: 8174
Taar8b
Name: trace amine-associated receptor 8B
Synonyms: LOC382348
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 382348
Homologene: 77586
Vmn2r32
Name: vomeronasal 2, receptor 32
Synonyms: V2r5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 22311
Homologene: 113703
Chil4
Name: chitinase-like 4
Synonyms: Ym2, Chi3l4
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 104183
Homologene: 74931
Acox3
Name: acyl-Coenzyme A oxidase 3, pristanoyl
Synonyms: pristanoyl-CoA oxidase, PCOX, EST-s59
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 80911
HGNC: HGNC:121
Homologene: 37792
Gm14295
Name: predicted gene 14295
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 100039123
Homologene: 133105
BC023105
Name: cDNA sequence BC023105
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 667597
VEGA: 18
Sox18
Name: SRY (sex determining region Y)-box 18
Synonyms: Sry-related HMG-box gene 18, Ragl
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 20672
Homologene: 7546
Mir7072
Name: microRNA 7072
Synonyms: mmu-mir-7072
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 102466800
VEGA: 8
AC161246.1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to G, chromosome 1 at 74,295,365 bp
  • A to T, chromosome 1 at 190,800,031 bp
  • A to C, chromosome 2 at 84,723,742 bp
  • A to G, chromosome 2 at 127,141,407 bp
  • G to A, chromosome 2 at 163,613,751 bp
  • G to T, chromosome 2 at 167,105,601 bp
  • A to C, chromosome 2 at 176,809,676 bp
  • G to A, chromosome 2 at 181,671,224 bp
  • A to G, chromosome 3 at 106,206,092 bp
  • A to G, chromosome 4 at 51,229,804 bp
  • C to T, chromosome 4 at 126,235,053 bp
  • A to T, chromosome 5 at 24,803,731 bp
  • T to C, chromosome 5 at 35,612,106 bp
  • T to A, chromosome 5 at 65,821,799 bp
  • T to A, chromosome 5 at 120,459,805 bp
  • T to C, chromosome 5 at 120,963,834 bp
  • C to A, chromosome 6 at 17,100,360 bp
  • G to T, chromosome 6 at 120,887,230 bp
  • A to G, chromosome 6 at 132,754,895 bp
  • A to G, chromosome 7 at 7,464,084 bp
  • A to C, chromosome 7 at 29,198,255 bp
  • T to A, chromosome 7 at 141,272,644 bp
  • T to G, chromosome 8 at 3,185,059 bp
  • T to G, chromosome 8 at 24,533,954 bp
  • T to C, chromosome 8 at 25,802,037 bp
  • C to T, chromosome 8 at 94,505,094 bp
  • T to C, chromosome 9 at 45,175,543 bp
  • T to C, chromosome 9 at 110,040,232 bp
  • C to T, chromosome 10 at 24,091,927 bp
  • C to G, chromosome 11 at 70,970,921 bp
  • T to A, chromosome 12 at 72,089,927 bp
  • C to A, chromosome 12 at 110,658,126 bp
  • A to T, chromosome 13 at 5,864,948 bp
  • A to G, chromosome 13 at 33,251,608 bp
  • T to A, chromosome 13 at 105,109,706 bp
  • A to T, chromosome 15 at 83,502,173 bp
  • A to G, chromosome 15 at 101,029,782 bp
  • T to A, chromosome 17 at 20,847,363 bp
  • T to A, chromosome 17 at 25,094,665 bp
  • C to A, chromosome 17 at 26,925,514 bp
  • C to G, chromosome 17 at 71,231,339 bp
  • T to A, chromosome 18 at 20,590,184 bp
  • T to C, chromosome 18 at 36,765,701 bp
  • T to A, chromosome 18 at 60,442,655 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R4949 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
042546-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.